Hepatic Cirrhosis Occurring in a Young Woodchuck(Marmota monax) Due to Vertical Transmission of Woodchuck Hepatitis Virus(WHV)

Kyu-shik Jeong,정원일,Jae-yong Chung,Mi-young An,Chae-yong Jung,Gyoung-jae Lee,Jong-soo Kang,Byeong-cheol Kang,Young-heun Jee,Bruce H Williams,Young-oh Kwon,Da-hee Jeong 대한수의학회 2003 Journal of Veterinary Science Vol.4 No.2

Cirrhosis Occurring in a Young Woodchuck (Marmota monax) Due to Vertical Transmission of Woodchuck Hepatitis Virus (WHV)Da-hee Jeong, Won-il Jeong, Jae-yong Chung, Mi-young An, Chae-yong Jung, Gyoung-jae Lee1, Jong-soo Kang1, Byeong-cheol Kang2, Young-heun Jee3, Bruce H Williams4, Young-oh Kwon5 and Kyu-shik Jeong*College of Veterinary Medicine, Kyungpook National University, Daegu 702-701, Korea1Shinwon Scientific Co., LTD, Research Institute, Suwon, Korea2Clinical Research Institute, Seoul National University, Seoul 110-744, Korea3College of Veterinary Medicine, Jeju National University, Jeju 690-756, Korea

병원 도착 전 사망 환자의 사인기재에 관한 고찰

강용선,이경룡,박인철,조광현,김승호,이한식 대한응급의학회 2001 대한응급의학회지 Vol.12 No.4

Background: To the emergency physician, issuing a death certificate is becoming a burden as the DOAs(Deaths on Arrival) have increased in recent years. We analyzed the agreement on the causes of death issued by emergency physicians and attempted to find out whether emergency physicians complied with the guidelines for issuing death certificates. Material & Method: A survey questionnaire containing twelve pre-selected DOA cases which were supplemented with relevant past medical history and physical examination was used. The cases, with varying causes of death, were chosen from the medical records of DOA patients who presented to the emergency department at Severance Hospital, Yonsei University College of Medicine, from January 1997 to December 2000. The questionnaires were sent to 60 emergency physicians(22 specialists and 38 residents) at 22 university-affiliated teaching hospitals and 2 general hospitals across the nation. They were asked to identify the most probable direct cause of death for each of the 12 cases. The same questionnaire was sent to medical examiners at the National Institute of Scientific Investigation and to a Korean emergency physician at Albert Einstein College of Medicine in New York, USA. We also included an open question about the optimal age for the use of 'senility' as a cause of death. Results: All 60 emergency physicians responded to the survey. The average number of causes of death per case was 9.7(7∼ 14). The range of concordance of causes of death was 23.3% to 66.6%. Out of a total of 720 causes of death given by the emergency physicians,35(4.9%) failed to adhere to the death certification guidelines, Also, 210 causes of death were not listed in the Korean classification of standard causes of death. Interestingly, the medical examiner answered 'unknown etiology' and the emergency physician in the USA answered 'cardiopulmonary arrest' or 'respiratory arrest' in most cases. Regarding 'senility' as a cause of death, 22 physicians(36.7%) thought the optimal age was over 80 years. Conclusion: A significant lack of agreement exists in determining the cause of death for the DOA patients arriving at emergency departments, Therefore, an all-out effort is essential to find ways to improve and resolve this situation. As the death certificate is a legal document and a basis for vital statistics, emergency physician should seek a rational consensus to improve and resolve these inconsistencies.

해수와 해산물로부터 Listeria 속의 분리와 세균학적 조사

강치희,이승철,황용일 慶南大學校 附設 基礎科學硏究所 2000 硏究論文集 Vol.14 No.-

본 연구는 경남지역의 해수와 해산물에서 Listeria sp.를 분리하여 증균배양방법에 따른 균의 분리율, 선택배지에 따른 균의 분리율, 검체에 따른 균종별 분리율, 생화학 시험과 분리된 L. monocytogenes에 대하여는 혈청형 시험을 실시하였다. 총 100건의 검체에서 증균배양에 따른 Listeria sp.의 분리율은 1일 증균배양에서 L. monocytogenes가 4%(4건), L. innocua가 35%(35건), L. ivanovii가 4%(4건), L. welshimeri가 1%(1건)였고, 7일간 증균배양에서는 L. monocytogenes가 7%(7건), L. innocua가 38%(38건), L. ivanovii가 5%(5건), L. welshimeri가 1%(1건)로 동정되어 1일 증균배양시키는 것보다 7일간 증균배양시키는것이 Listeria sp.의 분리에 훨씬 효과적이었다. 2가지 선택배지, Oxford 선택배지, LPM 선택배지를 사용하였을 경우 증균배양과 비교하여 분리율이 조금 낮았다. 검체에 따른 Listeria sp.의 분리율은 새우에서 80%(16/20)와 가시발새우에서 80%(16/20)로 분리율이 가장 높았으며, 바지락에서 50%(10/20), 해수에서 25%(5/20), 홍합에서 20%(4/20)의 순으로 나타났다. 그리고 Listeria sp.의 균종별 분리율은 L. innocua가 38%(38건), L. monocytogenes가 7%(7건), L. ivanovii가 5%(5건), L. welshimeri가 1%(1건)순으로 분리되었다. 각 검체에서 Listeria sp.의 균종별 분리율은 새우에서 L. innocua가 65%(13건), L. monocytogenes가 15%(3건), 가시발새우에서 L. innocua가 70%(14건), L. monocytogenes가 10%(2건), 바지락에서 L. innocua가 45%(9건), L. monocytogenes가 5%(1건), 홍합에서 L. innocua가 10%(2건), L. monocytogenes가 5%(1건), L. welshimeri가 5%(1건), 해수에서 L. ivanovii가 25%(5건) 분리되었다. 새우와 가시발새우에서 L. monocytogenes와 L. innocua가 다소 많이 분리되었다. L. monocytogenes로 분리 동정된 7주에 대한 혈청형 분류는 type 1이 2주, type 4가 5주로 나타났다. Four species of the genus of Listeria were isolated from seawater and sea food in Kyungnam area. These strains were systematically counted for different samples using appropriate media and biochemical tests including serological test. In one-day enrichment, the following strains were found out of 100 samples : L. innocua (35%), L. ivanovii (4%), L. monocytogenes (4%), and L. welshimeri (1%), In seven-day enrichment. L. innocua (38%), L. ivanoii (5%), L. monocytogenes (7%), and L. welshimeri (1%) were isolated. Thus, listeria species were more easily isolated in the seven-day enrichment than in the one-day enrichment. However, listerias were less grown in the selective medium than in the enrichment medium. Listeria species were most abundantly isolated in shrimps (80%) and metapenaeus shrimps (80%). Little neck clams (50%), seawater (25%) and hard-shelled mussels (20%) followed. The isolated listerias consisted of L. innocua (38%), L. monocytogenes (7%), L. ivanoii (5%), and L. welshimeri (1%). In the shrimps, L. innocua (65%) was most abundant followed by L. monocytogenes (5%). In the metapenaeus shrimps, 70% of the isolates were for L. innocua and 10% for L. monocytogenes. In the little neck clams, 45% was for L. innocua and 5% for L. monocytogenes. In the hard shelled mussels, 10% was for L. innocua, 5% for L. monocytogenes and 5% for L. welshimeri. In the seawater, 25% was for L. ivanovii. Therefore, L. monocytogenes and L. innocua were more frequently isolated in the shrimps and the metapenaeus shrimps. The serological classes for the seven L. monocytogenes were two strains for type I and five strains for type IV.

표면근전도와 전자각도기를 이용한 자동차 부품 조립작업 위험요인 평가

강동묵,이철호,,신용철,김은아,우지훈,조병만,이수일 大韓産業醫學會 2005 대한직업환경의학회지 Vol.17 No.4

Objectives: As automobile part manufacturing is characterized by high speed and high repetition, observation methods which are usually utilized for static posture are inappropriate to evaluate musculoskeleatal risk factors. This study quantified the risk factors of musculoskeletal disorders on the forearm and suggested exposure limits by estimating the risk factors using surface electromyography (EMG) and electrogoniometer. Methods: Ten percent of the total workers at 3 automobile part manufacturing factories were randomly selected, and 99 male workers were recruited as study subjects. The study was conducted during May 2003 to September 2004. The workers were equipped with electrogoniometers on the wrist and the elbow, surface EMGs on the skin of the flexor digitorum superficialis (FDS) and extensor carpi radialis (ECR) muscles, and the heart beat recorder during work as indicators of joint movement, local muscle tension and physical work load, respectively. Results: After controlling for age, body mass index and job stress, wrist flexion maximum angle, FDS relative activity (RA) and ECR RA were significantly associated with forearm musculoskeletal symptoms. The odds ratios of the forearm were 5.0(95% CI: 1.1-22.7), 14.0(95% CI: 1.5-128.8) and 7.3(95% CI: 1.1-49.4) for wrist flexion maximum angle more than 76˚, FDS RA more than 2.8%, and ECR RA more than 3.5%, respectively. Conclusions: Joint angle and focal muscle activity were associated with forearm musculoskeletal symptoms. To reduce forearm musculoskeletal symptoms among automobile part manufacturers, the wrist flexion angle, and FDS and ECR activity need to be reduced below the guidelines recommended in this study.

해수와 해산물에서 분리된 Listeria 속에 대한 항균제 감수성시험과 Inactivation 시험

강치희,이승철,황용일 慶南大學校 附設 基礎科學硏究所 2000 硏究論文集 Vol.14 No.-

주요 식중독균으로 주목받고 있는 Listeria sp.를 경남지역의 해수와 해산물에서 분리하여 항균제 감수성시험, 온도에 대한 균의 감수성 시험, 시험균주의 증식곡선, 내염성 시험과 pH에 대한 균의 증식관계를 조사하였다. 해수와 해산물에서 분리동정된 18주의 Listeria sp.에 대하여 디스크 확산방법으로 항균제 감수성 시험을 실시하여 ampicillin에 55.6%, cephalothin에 100%, chloramphenicol에 100%, clindamycin에 100%, erythromycin에 100%, gentamicin에 100%, kanamycin에 77.8%, methicillin에 83.3%, streptomycin에 50%, tetracycline에 83.3%, vancomycin에 100%의 감수성을 각각 보였다. 온도에 대한 증식억제효과는 55℃에서 30분간 처리에서는 영향이 없으나 60℃에서는 L.monocytogenes Type 1과 L.monocytogenes Type 4, L. ivanovii, L. welshimeri는 20분 처리로 사멸하였으며 L.monocytogenes ATCC 19111과 L. monocytogenes Scott A, L. innocua는 25분 처리로 사멸하였다. 65℃처리에서는 L. monocytogenes Type 4와 L. innocua, L. ivanovii는 15분에서 사멸하였으며 L. monocytogenes ATCC 19111과 L. monocytogenes Scott A, L. monocytogenes Type 1, L. welshimeri는 20분간 처리로 사멸하였다. 분리된 Listeria sp.의 내염성은 8% 이상에서는 지연된 유도기가 관찰되었으며 6% 염을 첨가한 0.6% TSA-YE배지에서는 정지기에서 흡광도(590nm)가 0.67에서 0.87의 범위에 있었으며, 8% 염에서는 정지기의 흡광도가 0.44에서 0.73이었고, 10% 염에서는 흡광도가 0.18에서 0.63이었으며, 12% 염에서는 OD 값이 0.03에서 0.2로 측정되어 염의 함량이 높을수록 저해 정도도 높았다. pH에 대한 증식영향은 pH 5.0에서보다 pH10에서의 증식이 빠른 것으로 보아 Listeria sp.는 비교적 높은 pH에서 생육가능 함을 보였다. Eighteen of Listeria species, a potent food poisioner, were isolated from sea food and seawater in Kyungnam area. These strains were tested for the sensitivities of antibiotics and temperature as well as for the resistances to high salt concentrations and the cell growth in different pHs and media compositions. The average sensitivities (%) of the eighteen listerias in disc diffusion method were following : ampicillin 55.6, cephalothin 100. chloamphenicol 100, clindamycin 100, erythromycin 100. gentamycin 100, kanamycin 77.8 methicillin 83.3, streptomycin 50, tetracyclin 83.3 and vancomycin 100. The viability of Listeria species was not so affected by heat treatment at 55℃ for 30 min. However, L. monocytogenes Type I and IV, L. ivanovii and L. welshimeri ceased to grow at 60℃ in 20 min. In contrast, the growth of L. monocytogenes ATCC19111, L. monocytogenes Scott A and L. innocua was affected in 25 min. L. monocytogenes type IV, L. innocua and L. ivanovii stopped growing at 65℃ in 15 min. L. monocytogenes ATTCC19111, L. monocytogenes Scott A, L. monocytogenes Type I, and L. welshimeri did not sustain at 65℃ for 20 min. The growth of the listerias were lagged in the presence of over 8% NaCl. In TSA-YE medium containing 6% NaCl, the optical density (OD) at 590mm of the cell culture reached stationary phase from 0.67 to 0.87. At 8% NaCl, the stationary phase OD of the culture was from 0.44 to 0.73. At 10% NaCl, the stationary phase OD of the culture was from 0.18 to 0.63. At 12% NaCl, the OD of the culture from 0.03 to 0.2. Therefore, higher salt concentrations created greater inhibition for the cell growth, Finally, the growth rates of the listereas were higher at pH 10 than at pH 5, which implies that the growth of the listerias was favorable in the alkaline pH.

뇌졸중후 연하곤란 환자의 식이 상태 비교

신용일,양충용,김헌수,강문규,주민철,길은영,권영미 圓光大學校 醫科學硏究所 2004 圓光醫科學 Vol.19 No.2

회맹장 및 대장을 침범한 장 결핵에 있어서 바륨을 이용한 방사선 이중조영촬영 소견에 대한 연구

진용현,전혜정,이용근,강철호,조성범,김윤환,박철민,차상훈,김일영,이혜경 건국대학교 의과학연구소 2002 건국의과학학술지 Vol.12 No.-

To determine the characteristic double contrast barium enema findings of ileo-cecal and colonic tuberculosis and to make differential diagnosis from other bowel inflammatory disease. Sixty-seven patients confirmed as ileo-cecal and/or colonic-tuberculosis in six hospitals were evaluated retrospectively. The diagnosis was proven by endoscopic biopsy, surgery, and clinical follow up. Nine of 67 patients were excluded because radiologic findings were normal though endocopic biopsy examinations resulted in tuberculosis. Histopathological diagnosis were madein 58 cases as follows: endoscopic biopsy(n=29), operation(n=12), follow up examination(n=14), tissue culture(n=1), laparoscopic biopsy(n=2). There were 30 men and 28 females. The mean age were 36 yrs(range:16-67), 34 patients were in the second and third decade. The distribution of lesions, morphological characteristics, mucosal changes, ulcer shape and depth, associated with other tuberculous lesion, multiplicity were retrospectively analyzed in the findngs of double contrast barim enema. The commonest clinical symptoms were abdominal pain(n=35, 60%) and diarrhea(n=18, 31%). The chest radiography revealed association of pulmonary tuberculosis in 39 cases. Tuberculous lesions of other organs except chest were present in five cases. Double contrast barium enema findings showed a thickening and irregularity of intestinal mucosal fold(n=29, 50%), bowel contraction and/or shortening(n=26, 45%). There were small, nodular, superficial ulcers in the case of ulcer depth less than 2 mm in the depth. As many as 39 cases(67%) in this series had two or more lesions in the intestinal tuberculosis. The involved segment revealed symmetrical feature(n=21, 36%). Inflammatory pseudopolypoid lesions were noted in 30 cases(52%). The ileo-cecal region was involved in 38 cases(66%). The ascending colon(n=25, 43%) was the second commonest site with or without contiguous cecal involvement. Lesions in terminal ileum, ileo-cecal valve, transverse colon, descending colon and appendix were seen in 19, 18, 18, 12 and seven cases, respectively. Among 18 cases of ileo-cecal valve lesions, there were incompetency in nine cases(16%) and wide gapping in four cases(7%). In 14 cases, tuberculous enterocolitis was diagnosed by clinical follow-up and double contrast barium enema study. 12 out of 14 cases, the findings of double contrast barium enema of tuberculous enterocolitis were normalized after anti-tuberculous medication. However, two cases showed more aggravated state than before due to inappropriate treatment. Double contrast barium enema plays an important role in the diagnosis of tuberculous enterocolitis, determines the type, site and extent of the involvement and helps in treatment guide by using sequential studies.

Canine juvenile cellulites의 진단과 치료 증례

황철용,유종현,강형석,윤화영,한홍율 한국임상수의학회 2002 한국임상수의학회지 Vol.19 No.4

Canine Juvenile cellulitis was diagnosed in 2 puppies hospitalized in Veterinary Medical Teaching Hospital of Seoul National University. Characterized dermatological problems were presents of scale, crust, purlent exudation and alopecia limited on their face. On cytologic examinations of direct impression smears for the lesions, numerous neutrophils and macrophagies were observed. No bacteria and fungus were noted. Treatments with administration of predinisolone and antibiotic orally combined with topical shampoo treatment had good results for the two puppies.

Fluoroquinolone 항생제에 교차반응을 보인 ofloxacin에 의한 아나필락시양 반응 1례

김철우,김덕영,류성태,김상용,장창수 대한천식 및 알레르기학회 2004 천식 및 알레르기 Vol.24 No.1

Fluoroquinolones are antimicrobial agents that have a broad range of activity against both gram-negative and gram-positive organisms. Anaphylactoid reactions have been sporadically reported with fluoroquinolones. There have been a few reports that describes cross-reactivity between fluoroquinolones. We experienced case of ofloxacin-induced anaphylactoid reaction, and confirmed cross-reactivity between ofloxacin and ciprofloxacin with the oral challenges test. Cross-reactivity between fluoroquinolones may be important, and avoidance of any fluoroquinolones should be mandatocy for patients with hypersensitivity reaction to one of these drugs. (J Asthma Allergy Clin Immunol 24 : 146-51, 2004)

통계 영역 수행평가 문항개발

김래선,강신조,박정희,최용철 전주대학교 교육문제연구소 2000 敎育論叢 Vol.15 No.-