Streptozotocin-당뇨병 백서에서 당뇨병 이환기간에 따른 대동맥의 이완능 저하 및 Oxygen-Free Radical에 의한 이완능의 손상정도

김정국,김신우,김수동,서예경,하승우,김보완,백운이,권삼,강승완 경북대학교 병원 1997 경북대학교병원의학연구소논문집 Vol.1 No.1

목적: 당뇨병의 이환기간이 혈관의 내피세포 의존성 및 비의존성 이완능의 저하에 미치는 영향과 이환기간에 따라 oxygen-free radical(OFR)에 의한 동맥이완능의 손상정도에 차이가 있는지를 알아보고자 하였다. 방법: Streptozotocin(STZ)으로 당뇨병이 유도된 3 주 및 10주째의 백서군과 연령이 비슷한 정상 대조백서군의 적출 대동맥환을 norepinephrine으로 수축시킨 상태에서 내피세포 의존성 이완제인 acetylcholine(ACh) 및 비의존성 이완제인 nitroglycerine(NTG)에 대한 이완능을 즉정하여 비교 관찬하였다. 또한 xanthine과 xanthine oxidase의 혼합으로 생성된 H₂O₂에 대동맥환을 노출시킨뒤 역시 ACh과 NTG에 대한 이완능의 손상정도를 비교하였다. 결과: 3주및 10주째 당뇨병 백서의 대동맥환은 모두 대조군 백서의 대동맥환에 비해 ACh에 대한 이완능이 유의하게 감소하였으며 10주째 당뇨병 백서군에서의 ACh에대한 이완능의 저하는 3 주째 당뇨병 백서군에 비해 유의하게 심하였다.H₂O₂에 노출시킨 뒤 ACh에대한 이완능은 3주 및 10주 째 당뇨병 백서군에서 모두 대조군에 비해 유의하게 심한 손상을 보였으나 3주와 10주째 당뇨병 백서군 사이의 손상정도의 차이가 통계학적으로 유의하지는 않았다. 한편 3주 및 10주째 당뇨병 백서군 모두 NTH에 대한 대동맥환의 이완능은 대조 백서군과 별다른 차이가 없었으며 또 H₂O₂에 노출시킨 뒤에도 NTG에 대한 이완능의 손상은 관찰되지 않았다. 결론: STZ-당뇨병백서에서 대동맥의 이완능은 당뇨병에 이환된지 3 주째에 이미 ACh에 대한 내피세포 의존성 이완능이 선택적으로 저하되며 당뇨병의 이환기간이 길수록 이완능의 저하가 심하였고, OFR에 의한 동맥 이완능의 손상도 내피세포 의존성 이완능에만 선택적으로 나타나나 이환기간에 따라 OFR에 의한 이완능 손상정도에는 뚜렷한 차이가 없음을 시사하고 있다. Objectives: Diabetes mellitus produces histological evidence of endothelial as well as changes in vascular function in experimental animal. Oxygen free radicals interfere with of destroy endothelium-dependent relaxation in normal and diabetic blood vessels. The present study was investigated whether the duration of diabetic state can affect the severity of impairment in endothelium-dependent and/or independent relaxation and the sensitivity to oxygen free fadical (OFR)-induced damage in diabetic rat vasculature. Methods: The relaxative responses of aortic ring precontracted with norepinephrine in vitro to cumulative concentrations of acetylcholine (ACh) and nitroglycerine (NTG) were conducted in 3- and 10- week streptozotocin (STZ)-diabetic rats and agematched controls. After exposure to H₂O₂generated by mixing xanthine and xanthine oxidase, the degree of impairment in relaxative responses to ACh and NTG were also compared between 3- and 10-week diabetic rats. Results: The relaxative responses of aortic rings produced by cumulative concentrations of ACh were significantly lower in both 3- and 10-week diabetic rats than in the age-matched controls. The impairments of relaxative responses to ACh in 10-week diabetic rats were significantly greater than those in 3-week ones. After H₂O₂ exposure, the impairments of relaxative responses to ACh in 3- and 10-week diabetic aortas was also significantly greater than those in the control in the control ones. However, the severity of H₂O₂-induced damage in 10-week diabetic aortas was not significantly different than that in 3-week ones. In contrast, aortic rings of 3- and 10-week diabetic rats could still be fully relaxed by NTG, even after exposure to H₂O₂. Conclusion: These findings suggest that in SRZ-diabetic rat aortas selective impairment of endothelium-dependent relaxation to Ach can be found early even over a 3-week period after the induction of diabetes and the impairment may be greater in those with the longer duration of the diabetic state and that the impairment of the vascular relaxation by OFR is also selective to endothelium-dependent response but the sensitivity of OFR-induced damage may not be related to the duration of the diabetic state.

Hepatitis C Virus Non-structural Protein NS4B Can Modulate an Unfolded Protein Response

Yi Zheng,Bo Gao,Li Ye,Lingbao Kong,Wei Jing,Xiaojun Yang,Zhenghui Wu,Linbai Ye 한국미생물학회 2005 The journal of microbiology Vol.43 No.6

Viral infection causes stress to the endoplasmic reticulum (ER). The response to endoplasmic reticulum stress, known as the unfolded protein response (UPR), is designed to eliminate misfolded proteins and allow the cell to recover. The role of hepatitis C virus (HCV) non-structural protein NS4B, a component of the HCV replicons that induce UPR, is incompletely understood. We demonstrate that HCV NS4B could induce activating transcription factor (ATF6) and inositol-requiring enzyme 1 (IRE1), to favor the HCV subreplicon and HCV viral replication. HCV NS4B activated the IRE1 pathway, as indicated by splicing of X box-binding protein (Xbp-1) mRNA. However, transcriptional activation of the XBP-1 target gene, EDEM (ER degradation-enhancing α-mannosidase-like protein, a protein degradation factor), was inhibited. These results imply that NS4B might induce UPR through ATF6 and IRE1- XBP1 pathways, but might also modify the outcome to benefit HCV or HCV subreplicon replication.

siRNA-mediated Silencing of Notch-1 Enhances Docetaxel Induced Mitotic Arrest and Apoptosis in PCa Cells

Ye, Qi-Fa,Zhang, Yi-Chuan,Peng, Xiao-Qing,Long, Zhi,Ming, Ying-Zi,He, Le-Ye Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.6

Purpose: Notch is an important signaling pathway that regulates cell fate, stem cell maintenance and the initiation of differentiation in many tissues. It has been reported that activation of Notch-1 contributes to tumorigenesis. However, whether Notch signaling might have a role in chemoresistance of prostate cancer is unclear. This study aimed to investigate the effects of Notch-1 silencing on the sensitivity of prostate cancer cells to docetaxel treatment. Methods: siRNA against Notch-1 was transfected into PC-3 prostate cancer cells. Proliferation, apoptosis and cell cycle distribution were examined in the presence or absence of docetaxel by MTT and flow cytometry. Expression of $p21^{waf1/cip1}$ and Akt as well as activation of Akt in PC-3 cells were detected by Western blot and Real-time PCR. Results: Silencing of Notch-1 promoted docetaxel induced cell growth inhibition, apoptosis and cell cycle arrest in PC-3 cells. In addition, these effects were associated with increased $p21^{waf1/cip1}$ expression and decreased Akt expression and activation in PC-3 cells. Conclusion: Notch-1 promotes chemoresistance of prostate cancer and could be a potential therapeutic target.

The development of an infant gait pattern

Yi, Kyung Ok,Kim, Mi Ye 한국유산소운동과학회 2000 한국유산소운동과학회지 Vol.4 No.1

유아기 자녀에 대한 양육스트레스와 모-자녀관계에서 어머니 취업상태의 조절효과

이예진(Yi, Ye-Jin),윤수정(Yoon, Soo-Jung) 한국영유아보육학회 2018 한국영유아보육학 Vol.0 No.109

본 연구는 유아기 자녀를 둔 어머니 양육스트레스와 모-자녀관계에서 어머니 취업여부의 조절효과가 있는지, 변인들의 관련성과 영향력을 살펴보고자 하였다. 연구대상은 경인지역 유아교육기관을 이용하는 만 3~5세 유아의 어머니 586명을 대상으로 하였다. 연구방법을 살펴보면, 양육스트레스(K-PSI-SF)와 모-자녀관계(K-PRQ-P) 도구를 토대로 한 설문지조사가 이루어졌고, 수집된 자료에 대해 t-test, 상관분석 및 위계적 회귀분석이 시행되어졌다. 연구결과에서 첫째, 취업모는 전업모에 비해 모-자녀관계의 양육효능감은 높고, 관계좌절감은 낮은 것으로 나타났으며, 양육스트레스의 부모고통은 취업모보다 전업모가 더 많이 받는 것으로 조사되었다. 둘째, 취업여부에 따른 양육스트레스와 모-자녀관계는 밀접한 관련성이 있는 것으로 나타났다. 셋째, 양육스트레스와 모-자녀관계에서 어머니 취업여부의 조절효과가 있는 것으로 조사되었다. 이와 같이, 모-자녀관계 증진을 위한 어머니 취업의 상황적 여건과 양육스트레스의 심리적 여건 모두를 고려한 개별화된 지원이 필요함을 시사할 수 있다. The purpose of this study is to investigate the moderating effect of employment status between parenting stress and mother-child relationships. The participants of this study were 568 mothers of 3, 4 and 5-year-old children who attend a kindergarten or daycare center in the Gyungin area of South Korea. The collected data were analyzed by descriptive statistics, t-test, Pearson’s correlation, and hierarchial regression using SPSS 18.0. The results are as follows. First, employed mothers showed higher parental confidence and lower relational frustration in mother-child relationships, and higher parental distress and parenting stress than non-employed mothers. Second, mother’s parenting stress and mother-child relationships showed a significant correlation from both employed and non-employed mothers. Third, the results indicate a moderating effect of employment status between parenting stress and mother-child relationships. This result suggests that the mothers employment status and parenting stress should be considered to promote mother-child relationships.

Mutations of TYR and MITF Genes are Associated with Plumage Colour Phenotypes in Geese

Wang, Ye,Li, Si-Ming,Huang, Jing,Chen, Shi-Yi,Liu, Yi-Ping Asian Australasian Association of Animal Productio 2014 Animal Bioscience Vol.27 No.6

The polymorphism of microphthalmia-associated transcription factor (MITF) and tyrosinase (TYR) genes have been proposed to play a vital role in coat colour genesis in mammals, but their role remains ambiguous in geese at best. Here, we cloned and sequenced 1,397 bp coding region of MITF gene and a 588 bp fragment of TYR exon 1 for polymorphism analysis among 157 domestic geese showing three types of plumage colour. We detected a total of three SNPs (c.280T>C, c.345G>A, and c.369G>A) in TYR and six haplotypes (H1-H6). Among them, haplotypes H1, H2, H3, and H5 were significantly associated with white plumage trait of Zhedong White Geese. However, only diplotype H1H1 and H3H5 were significantly associated with white plumage trait of Zhedong White Geese (p<0.01). We only detected one SNP (c.1109C>T) for MITF gene and found that genotype CT and TT were significantly associated with white plumage trait of Zhedong White Geese. Briefly, our study suggested an association between polymorphisms of TYR and MITF genes and the plumage colour trait in domestic geese.

일반논문 : 선암사(仙巖寺) 소장 극사실주의(極寫實主義) 진영 연구

이예성 ( Ye Seong Yi ) 전남대학교 호남학연구원 2011 호남학 Vol.0 No.50

20세기 전반기에 제작된 극사실주의 진영은 형식화에 빠져 전통양식의 답습에 머물러 있던 진영에 획기적인 변화를 가져왔다. 이러한 변화는 불교가 민족종교로써 위상을 회복하고 대중과 함께 하는 종교로 거듭나기 위해 노력하는 가운데 일반회화에서 일어났던 변화를 적극 수용하였기 때문이다. 진영에 일반 초상화양식을 수용할 수 있었던 것은 진영이 불화 가운데 가장 일반회화와 친연성이 있고 초상 화와는 오래 전부터 서로 영향을 주고받았던 것과 관련이 있다. 극사실주의 진영은 오랜 진영의 역사에서 보면 표현 기법, 형식에 있어 가히 획기적인 변화라고 할 수 있다. 근대기에 그려진 극사실주의 진영 가운데 반신상으로 제작된 선암사 소장 극사실주의 진영은 현재 조사된 바로는 가장 먼저 나타난 양식으로 일반화가에 의해 그려졌다. 선암사 극사실주의 진영은 반신상을 그린 것인데, 반신상 진영은 몇 점 되지 않으며, 1930년 이후에는 그려지지 않았던 것으로 추정된다. 여러 요인이 있겠지만 아마도 오랜 전통과 전혀 다른 양식과 진영의 원래 취지 즉 감계, 추모, 예배, 존숭의 대상이라는 것에서 볼 때 반신상은 널리 받아들여지기 어려웠던 점이 있었던 것으로 보인다. 선암사 극사실주의 진영은 근대적 사실주의에 입각한 새로운 양식이라고 할 수 있다. 즉 이 진영들은 사진을 기반으로 하고 있으며, 면위주의 정밀한 묘사로 객관적 사실성을 추구하는 근대 미술의 보편적 특성을 보이고 있다. 이렇게 전통양식과는 전혀 다른 면을 보이는 선암사 극사실주의 진영은 이전의 형식주의에서 벗어나 근대 진영으로의 새로운 변화를 주도했다는 점에 큰 의의가 있다. 오래 전부터 초상화와 진영은 서로 영향을 주고받으며 발전해왔던 것을 고려하면 근대기의 진영이 형식화에 빠진 안일한 태도에서 벗어나기 위해 새로운 일반초상화의 양식을 수용했던 것은 자연스러운 일이라고 하겠으며, 결국 극사실주의 진영양식은 전통 진영과 일반회화와의 연계 속에서 하나의 시대 양식으로 성립되면서 현대 진영으로 이어져왔다고 하겠다. Hyperrealism(superrealism) Jinyoung(眞影) produced during the first half of 20th century brought epoch-making changes to the Jinyoung which at that time fell into formalization and followed the style of traditional ones. The change was brought along as a result of Buddhism positively accepting the changes of general painting, while redeeming its status as national religion and making efforts to become popular religion. Acceptance of general portrait style to Jinyoung was possible because Jinyoung has the most affinity with general paintings among Buddhist paintings and it has a history of exchanging influence with general portrait. In the history of Jinyoung, hyperrealism Jinyoung itself is an epoch-making change in expression technique and style. Hyperrealism Jinyoung in Seonamsa(仙巖寺) which was made half-length and produced by a general painter is, according to up-to-date investigation, the first hyperrealism style to appear among hyperrealism Jinyoungs produced in modern times. But there are few of half-length Jinyoungs, and it is estimated their style disappeared after 1930. There are many possible factors but the severance may be attributed to difficulty that half-length portrait had in being accepted; its style and purpose was completely different from traditional ones. Hyperrealism Jinyoung in Seonamsa(仙巖寺) is a new style grounded on modern realism. In other words, this Jinyoung shows style completely different from traditional one in that they are based on photograph and show general characteristic of modern paintings which pursue objective realism by detailed description. And it also has great significance in that it broke away formalism and led the epoch-making change to modern style Jinyoung. In regard to the historical relationship between general portrait and Jinyoung, it is natural that modern Jinyoung accepted new style of general portrait so as to get out of complacency of formalization. In short, hyperrealism Jinyoung was established as a style of the time in the process of connection with traditional Jinyoung and general painting and its style continues to contemporary Jinyoung.

전환기 청소년의 개인특성, 또래애착 및 부모양육행동이학교생활 적응에 미치는 영향: 휴대전화 의존도의 매개효과 검증

이예진 ( Ye Jin Yi ) 대한가정학회 2014 Human Ecology Research(HER) Vol.52 No.5

This study explores how the main variables of youth going through a transition period (parents` nurturing behavior, the youth`s self-esteem and ego resilience, the peer group relationships) affect the youth`s school adjustment. It also analyzes the effects of theses variables on the youth`s dependence on cell phones, which are considered a necessity in modern society. This research is verified by using a structure model verification method based on data from the Korean Children Youth Panel Study. According to the research, first, 1st-year students in middle school show better adjustment to their school life and less dependence on cell phones when they have a stronger sense of self-esteem and ego resilience; further, it is revealed that cell phone dependence has an indirect influence on the school life of these children. Second, they tend to show better adjustment to their school life and less dependence on cell phones when their parents` nurturing behaviors are more positive. Third, these children tend to depend more on cell phones when they have a positive peer group relationship, Moreover, cell phone dependence has an indirect influence on their school life. When considering the impact of cell phone to youth in modern society, need to prepare proper training program for correct uses and preventing from addiction.

교사 효능감과 유아의 부정적 또래상호작용 간의 유아-교사 상호작용의 매개효과

이예진(Yi, Ye Jin),윤수정(Yoon, Soo Jung) 한국영유아보육학회 2016 한국영유아보육학 Vol.0 No.101

본 연구는 교사 효능감과 유아의 부정적 또래상호작용 간의 관계에서 유아-교사 상호작용의 매개효과를 알아보는데 그 목적을 두었다. 연구대상은 육아정책연구소의 한국아동패널 6차년도(2013년)에 조사한 어린이집 또는 유치원에 다니는 만 5세아 1,163명과 그들의 담임교사이다. 연구방법은 교사 효능감과 유아의 부정적 또래상호작용 간의 관계에서 유아-교사 상호작용의 매개효과를 알아보기 위하여 AMOS 22.0 프로그램과 SPSS-WIN 18.0프로그램을 사용하여 시행하였다. 연구결과를 살펴보면, 교사 효능감이 높을수록 유아의 부정적 또래상호작용은 감소하며 유아-교사 상호작용은 질적으로 향상되는 것으로 나타났다. 또한 교사 효능감과 유아의 부정적 또래상호작용 간의 관계에서 유아-교사 상호작용은 완전매개효과를 보이는 것으로 조사되었다. The purpose of this study is to investigate the mediating effects of child-teacher interactions on teacher efficacy and children’s negative peer interactions. For this study, the data of the 6th Panel Study of Korean Children (PSKC) in 2013, collected from 1,163 5-year-old attending day care centers and kindergartens, and their teachers were used. The data were analyzed using structural equation modeling, using AMOS 22.0 and SPSS-WIN 18.0. The results indicated that higher teacher efficacy was related to a low level of children’s negative peer interactions and greater child-teacher interactions. In addition, the full spectrum of the child-teacher interactions mediating effects were seen to influence teacher self-efficacy and children’s negative peer interactions.

A Study of the Cultural Policy of the Communist Party of China towards Ethnic Minorities in the New Century

Xing Yi Ye,Bo Wang 한국문화정책학회 2015 문화정책 Vol.2 No.2

China has been a united, multi-ethnic country since ancient times, in addition to the Han, 55 ethnic minorities have created other ethnic cultures with their own rich content, distinctive features, and diverse forms in the long history of development. The Communist Party of China (CPC) has attached great importance to the culture construction of ethnic minority since its foundation. In the new century, China views the prosperity of ethnic culture is an important part of the implementation of the scientific concept of development and the comprehensive construction of a well-off society. This also endows ethnic minority cultures with new contents. This article not only discusses the new contents but also surveys the development process of cultural policy of ethnic minorities and sums up the historical experiences of the development of cultural policy covering ethnic minorities in China.