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Promoter Analysis of Bombyx mori Nucleopolyhedrovirus Ubiquitin Gene
Xu’ai Lin,Yin Chen,Yongzhu Yi,Jie Yan,Zhifang Zhang 한국미생물학회 2008 The journal of microbiology Vol.46 No.4
The aim of this study was to analyze the characteristics of Bombyx mori nucleopolyhedrovirus (BmNPV) ubiquitin gene promoter and the effects of conserved motifs, such as TAAG, TATA, and CAAT, along with baculovirus enhancer homologous region 3 (hr3), on promoter activity. Ubiquitin gene of BmNPV was expressed during the late phase of virus infection. In the presence of viral factors, significant reduction of promoter activity was observed by deletion of -382 to -124 bp upstream of ATG. The fragment between -187 and -383 bp upstream of ATG, including distal TAAG, CAAT motif, and TATA box, could also drive expression of the reporter gene. The mutation of cis-elements TATA boxes and TAAG motifs significantly decreased the promoter’s activity, while CAAT mutations enhanced promoter activity by 2- or 3-fold, as compared with the native promoter. In the presence of BmNPV, hr3, both located downstream of the reporter gene of the same vector, and separate vector, could significantly enhance transcription activity of ubiquitin promoter as compared to the control. We concluded that BmNPV ubiquitin gene might be regulated by dual sets of promoter elements, where TAAG and TATA box may positively regulate the expression of ubiquitin, while CAAT motif functions as a negative regulator. Viral factor(s) play an important role in the co-activation of hr3 and promoter.
Preparation of lithium-doped NaV6O15 thin film cathodes with high cycling performance in SIBs
Xu Hai-Yan,Ruan Jun Hai,Liu Fang Lin,Li Dong-Cai,Zhang Feng-Jun,Wang Ai-Guo,Sun Dao-Sheng,오원춘 한국세라믹학회 2022 한국세라믹학회지 Vol.59 No.3
Lithium ions-doped NaV6O15 thin films have been prepared using a simple low temperature liquid phase deposition method and subsequent annealing process. X-ray diffraction (XRD), Fourier transform infrared spectrometer (FTIR), scanning elec- tron microscopy (SEM), and photoelectron spectroscopy (XPS) have been used to study the structural and physicochemical characteristics of the NaV6O15 film. The films were grown on the FTO conductive glass and used directly as an electrode of sodium ion batteries. The prepared lithium ions-doped NaV6O15 thin film electrodes showed an excellent cycling stability and discharge capacity, which may be attributed to the stability of the Li+ embedded into the gap between the V–O layers to maintain the structure and its stable β-phase structure transformed after the first cycle. The cycling stability greatly improved with increasing annealing temperature, while the discharge capacity decreased. The capacities of the film electrodes annealed at 400 °C and 450 °C maintained above 97% after 100 cycles. The lithium-doped NaV6O15 underwent a phase transition dur- ing the first charge/discharge cycle. The new transformed phase has perfect crystal structure stability undergoing insertion and deinsertion of Na+. Therefore, the lithium-doped NaV6O15 thin film possesses good cycling stability and is expected to be a promising thin film cathode for sodium-ion batteries.
Cheng Xu,Shi-Ping Yang,Yuan Zhang,Ling-Long Tang,Guan-Qun Zhou,Xu Liu,Yan-Ping Mao,Rui Guo,Wen-Fei Li,Lei Chen,Ai-Hua Lin,Ying Sun,Jun Ma 대한암학회 2018 Cancer Research and Treatment Vol.50 No.3
Purpose The purpose of this study was to investigate the effect of neutropenia during the first cycle of induction chemotherapy (IC-1) on survival in locoregionally advanced nasopharyngeal carcinoma (LANPC). Materials and Methods Eligible patients (n=545) with LANPC receiving IC+concurrent chemoradiotherapy were included. Based on nadir neutrophil after IC-1, all patients were categorized into three groups: no/grade 1-2/grade 3-4 neutropenia. Five-year overall survival (OS) and disease-free survival (DFS) were compared between groups and subgroups stratified by IC regimen. We also explored the occurrence of IC-1–induced myelosuppression events and the minimal value of post-treatment neutrophil-to-lymphocyte ratio (post-NLRmin). Univariate/multivariate analyses were performed to investigate the effect of IC-1–induced neutropenia, timing of neutropenia, number of myelosuppression events, and high post-NLRmin on OS/DFS. Results Grade 1-2/grade 3-4 neutropenia were associated with poorer OS/DFS than no neutropenia (all p < 0.05); OS/DFS were not significantly different between patients experiencing grade 1-2 vs. 3-4 neutropenia. Neutropenia had no significant effect on OS/DFS in patients receiving docetaxel–cisplatin–5-fluorouracil (TPF). Grade 1-2 (grade 3-4) neutropenia negatively influenced OS/DFS in patients receiving cisplatin–5-fluorouracil (PF) (PF and docetaxel– cisplatin [TP]; all p < 0.05). Neutropenia, two/three myelosuppression events, and high post-NLRmin ( 1.33) was most frequent on days 5-10, second and third week of IC-1, respectively. After adjustment for covariates, IC-1–induced neutropenia, two/three myelosuppression events, and post-NLRmin 1.33 were validated as negative predictors of OS/DFS (all p < 0.05); timing of neutropenia had no significant effect. Conclusion Occurrence of neutropenia, number of myelosuppression events, and high post-NLRmin during PF/TP IC-1 have prognostic value for poor survival in LANPC.
Surface nanocrystallization and its properties of a rare earth magnesium alloy induced by HVOF―SMB
Kai-dong Xu,Ji-na Wang,Ai-hua Wang,Hua Yan,Xiang-lin Zhang,Zao-wen Huang 한국물리학회 2011 Current Applied Physics Vol.11 No.3
The nanocrystalline microstructure in the surface of a rare earth magnesium alloy induced by a new process named HVOF―SMB (high velocity oxygen-fuel flame supersonic microparticles bombarding) has been characterized by means of X-ray diffractometry (XRD) and high-resolution transmission electron microscopy (HRTEM). The effects of HVOF―SMB on mechanical properties and corrosion resistance of the magnesium alloy have been investigated by microhardness distribution and polarization curves, and the cross-sectional observation of the treated Mg alloy sample has been characterized by optical microscopy (OM). Results showed that HVOF―SMB can induce surface nanocrystallization with a grain size level less than 20 nm at topmost surface layer, and the microhardness at the top surface layer increases up to about 170 HV_0.025 which is twice more than that of the substrate. The grain size increases as well as the microhardness decreases gradually with the depth varying from surface to substrate. The potentiodynamic polarization curves indicate that the HVOF―SMB treated surfaces show lower corrosion resistance in comparison with the untreated magnesium alloy in 3.5% NaCl solution. The underlying mechanism of the electrochemical properties on the nanocrystalline Mg-based alloy’s surface induced by severe plastic deformation in corrosion resistance is discussed.