RISS 학술연구정보서비스

다국어 입력

http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.

변환된 중국어를 복사하여 사용하시면 됩니다.

  • 中文 을 입력하시려면 zhongwen을 입력하시고 space를누르시면됩니다.
  • 北京 을 입력하시려면 beijing을 입력하시고 space를 누르시면 됩니다.
    인기검색어 순위 펼치기

    RISS 인기검색어

      검색결과 좁혀 보기

      • 좁혀본 항목

      • 좁혀본 항목 보기순서

        • 원문유무
        • 음성지원유무
          • 원문제공처
          • 등재정보
          • 학술지명
          • 주제분류
          • 발행연도
          • 작성언어
          • 저자

        오늘 본 자료

        • 오늘 본 자료가 없습니다.
        • 무료
        • 기관 내 무료
        • 유료

          Species identity of Phellinus linteus (sanghuang) extensively used as a medicinal mushroom in Korea

          Han, J. G.,Hyun, M. W.,Kim, C. S.,Jo, J. W.,Cho, J. H.,Lee, K. H.,Kong, W. S.,Han, S. K.,Oh, J.,Sung, G. H. MICROBIOLOGICAL SOCIETY OF KOREA 2016 The journal of microbiology Vol.54 No.4

          <P>Sanghuang is a medicinal mushroom that has gained particular attention in Korea. It has been extensively studied for the past few decades as a natural immune booster and cancer suppressor. Although the scientific name, Phellinus linteus, has been commonly used to refer to the sanghuang mushroom, the species identity of sanghuang has been called into question due to the ambiguity of its circumscription and the inadequacy of morphological distinctions within allied species. Because the species concept of sanghuang has been elucidated by recent molecular phylogenetic studies, it has become necessary to clarify the taxonomic positions of sanghuang strains extensively utilized in Korea. We conducted a phylogenetic analysis of 74 strains belonging to the P. linteus-baumii complex based on ITS nrDNA sequences. Parental stains of sanghuang varieties formally registered in the Korea Seed & Variety Service, including ASI 26046 (Corea sanghuang), 26114 (Boolro), and 26115 (HK 1-ho) were grouped with Sanghuangporus sanghuang instead of P. linteus in the inferred phylogeny.</P>


          New record and enzyme activity of four species in Penicillium section Citrina from marine environments in Korea.

          Park, Myung Soo,Eom, Ji Eun,Fong, Jonathan J,Lim, Young Woon Microbiological Society of Korea 2015 The journal of microbiology Vol.53 No.4

          <P>Several strains of Penicillium section Citrina were isolated during a survey of fungi from marine environments along the southern coast of Korea. Based on multigene phylogenetic analyses (β-tubulin and calmodulin) and morphological characteristics, the 11 strains were identified as P. citrinum, P. hetheringtonii, P. paxilli, P. sumatrense, P. terrigenum, and P. westlingii. To understand the ecological role of these species, we tested all strains for extracellular enzyme activity; six strains representing four species showed β-glucosidase activity. Four of the identified species - P. hetheringtonii, P. paxilli, P. terrigenum, and P. westlingii - are new records for Korea. For these new species records, we describe morphological characteristics of the strains and compare results to published data of type strains.</P>


          Serotyping of Cryptococcus neoformans Strains Isolated in Korea

          Hwang, Soo-Myung The Microbiological Society of Korea 2002 The journal of microbiology Vol.40 No.2

          Twenty strains of Cryptococcus neoformams isolated from environmental and clinical sources in Korea were examined for their serotypes. Two environmental isolates from pigeon excreta belonged to C. neoformans var. neoformans serotypes A. Of the 18 isolates from clinical specimens, 17 belonged to C. neoformans vats, neoiomans (serotype A : 16, serotype D : 1) and one belonged to C. neoformans vats, gattii serotype B, which was culturally unusual, producing mucous colonies. This is the first report of the identification of C. neoformans roar, gattii serotype B from a patient in Korea.


          Characteristics of ustilago maydis virus of SH14 killer strain isolated in Korea

          Hwang, Seon-Hee,Jung, Cheong-Hwan,Yie, Se-Won The Microbiological Society of Korea 1995 The journal of microbiology Vol.33 No.2

          SH-14, a novel killer strain of Ustilago maydis was isolated in Korea. It has been reported in other papers that the toxin specificity and double-stranded RNA pattern of SH-14 strain were different from other laboratory strains. In this paper, we analyzed the biochemical characteristics of U. maydis SH-14 virus. Three distinctive peaks were isolated from CsCl density gradient, designated as top (T), intermediate (I) and bottom (B) components. We found that the densities of each components, 1.285, 1.408 g/cm$\^$3/, respectively, are very similar to those of other strains. As previously reported by the analysis of dsRNA in each component, the dsRNA segments are separately encapsidated. Capsid protein of SH-14 virus consists of two proteins about 70 Kd shown by SDS-PAGE analysis. Electron microscopic examination of the virus particles revealed that UmV particles are very similar in size and morphology to all isolates as well as all lab-strains. In order to test immunological cross reactivity of UmV, werstern bolt analysis was carriedout with antiserum against A8 virus. All capsid protein had positive reaction against A8 antibody which indicated that UmV are immunologically cross-reactive with all isolates from Korea. The results presented in this paper may show that UmV isolated from SH-14 strain has very similar biochemical characteristics to those of other UmV. However, the difference in the toxin specificity and the molecular weight of toxin protein from the SH-14 strain has us to conclude that U. maydis SH-14 strain is a new killer type.

        • SCOPUSKCI등재

          Variation in Trichothecene and Zearalenone Production by Fusarium graminearum Isolates form Corn and Barley in Korea

          Kim, Jin-Cheol,Park, Ae-Ran,Lee, Yin-Won,Youn, Hee-Ju,Cha, Seung-Hee The Microbiological Society of Korea 1993 미생물학회지 Vol.31 No.4

          강원도와 남부지방에서 각각 옥수수와 보리를 채집하여 두 곡류로 부터 총 110 균주의 Fusatium graminearum을 분리한 후 쌀배지에서의 trichothecene과 zearalenone(ZEA)의 생성능을 측정하였다. 옥수수로 부터 분리한 51개의 F. graminearum 균주중 trichothecene 생성빈도는 deoxynivalenol(Don)이 64.5%, 3-acetyldeoxynivalenol (3-ADON)이 7.8%, 15acetyldeoxynivalenol (15-ADON)이 33.3%, nivalenol(NIV)이 21.6%, 4-acetylnivalenol (4-ANIV)이 13.7%였다. DON을 생성하는 균주들은 3-ADON보다는 15-ADON을 동시에 생성하였다. 한편, 보리로부터 분리한 59개의 F. graminearum 균주중 trichothecene 생성빈도는 NIV가 71.2%, 그리고 4-ANIV가 61.0%였으며, 한 균주만이 DON과 3-ADON을 동시에 생성하였다. 옥수수로부터 분리한 균주들의 ZEA의 생성빈도와 생성량은 각각 32.0%, 71㎍/g였으며, 보리로부터 분리한 균주는 각각 29%, 745㎍이었다. 이와같이 우리나라 강원도지방과 남부지방의 F. graminearum 균주들의 trichothecene 생성양상은 지역적인 큰 차이를 나타내었다. A total of 110 Fusarium graminearum isolates were obtained from corn and barley samples which were collected from Kangwon province and the southern part of Korea, respectively. The isolates were tested for trichothecene and zearalenone (ZEA) production in rice culture. The incidences of trichothecene production by 51 isolates of F. graminearum from corn were 64.7% for deoxynivalenol (DON), 7.8% for 3-acetyldeoxynivalenol (3-ADON),33.3% for 15-acetylde-oxynivalenol (15-ADON), 21.6% for invalenol (NIV), and 13.7% for 4-acetylnivalenol (4-ANIV). DON producers frequently co-produced 15-ADON rather than 3-ADON. On the other hand, the incidences of trichothecene production by 59 isolates of F. graminearum from barley were 71.2% for NIV, 61.0% for 4-ANIV, and only one isolate produced DON and 3-ADON. The incidences and mean levels of ZEA producers were 32.0% and 71.$\mu$g/g for the isolates from corn, and 29.0% and 74 .$\mu$g/g for the isolates from barley. There was a great regional difference in trichothecene production of F. graminearum isolates between Kangwon province and the southern part of Korea.


          High Prevalence of the China 1 Strain of Epstein-Barr Virus in Korea as Determined by Sequence Polymorphisms in the Carboxy-Terminal Tail of LMP1

          Cho, Sung-Gyu,Lee, Won-Keun The Microbiological Society of Korea 2003 The journal of microbiology Vol.41 No.2

          The Epstein-Barr virus (EBV)-encoded latent membrane protein 1 (LMPI) exhibits considerable sequence heterogeneity among EBV isolates. Seven distinct EBV strains have been defined based on sequence polymorphisms in the LMPI gene, which are designated China 1, China 2, China 3, Alaskan, Mediterranean, NC, and the B95-8 strains. In this study, we analyzed a 30-bp deletion and sequence variations in the carboxy-terminal region of the LMPl gene in 12 EBV isolates from spontaneous lym-phoblastoid cell lines derived from individuals with non-EBV associated cancers in Korea. Eleven of the 12 isolates showed a 30-bp deletion spanning LMPI amino acids 342 to 353, suggesting a high prevalence of the LMPI 30-bp deletion variant among EBV isolates in Korea. In addition, all 12 isolates had a 15-bp common deletion in the 33-bp repeat region and multiple base-pair changes relative to the prototype B95-8 EBV strain along with variations in the number of the 33-bp repeats. The bp changes at positions 168746, 168694, 168687, 168395, 168357, 168355, 168631, 168320, 168308, 168295, and 168225 were highly conserved among the isolates. Comparative analysis of sequence change patterns in the LMPI carboxy-terminal coding region identified nine 30-bp deletion variants as China 1, two deletion variants as a possible interstrain between the Alaskan and China 1 strains, and a single undeleted variant as a possible variant of the Alaskan strain. These results suggest the predominance of the China 1 EBV strain in the Korean population.

        • KCI등재

          2010년도 국내 임상에서 분리한 다제내성 녹농균의 유전자형 조사

          김민지,차민경,이도경,강주연,박재은,김영희,박일호,신혜순,하남주,Kim, Min Ji,Cha, Min Kyeong,Lee, Do Kyung,Kang, Ju Yeon,Park, Jae Eun,Kim, Young Hee,Park, Il Ho,Shin, Hea Soon,Ha, Nam Joo The Microbiological Society of Korea 2012 미생물학회지 Vol.48 No.4

          녹농균은 특히 면역이 저하된 환자에게서 심각한 감염을 일으키는 그람음성의 기회감염 균주이다. 또한 carbapenem 내성 metallo-${\beta}$-lactamases (MBL)를 가진 녹농균이 한국에서 증가되는 추세로 보고되고 있다. 따라서 본 실험에서는 2차 병원인 삼육 서울 병원에서 수집된 총 92종의 임상 녹농균의 다재내성 수준을 분석하였다. 항생제에 대한 감수성은 최소억제농도(MIC) 분석에 의해 결정되었고, inhibitor-potentiated disk diffusion(IPD) 분석은 MBL 검출을 위해 수행되었다. RAPD-PCR은 임상환자에서 분리한 녹농균 계통의 유전적 유형의 특징을 밝히기 위해 사용되었다. 그 결과 임상에서 분리된 녹농균의 40.2%는 ceftazidime에 내성을, 58.7%는 meropenem에 내성을, 56.5%는 gentamicin에 내성을, 46.7%는 tobramycin에 내성을, 62.0%는 ciprofloxacin에 내성을 그리고 97.8%는 chloramphenicol에 내성을 보였다. IPD 분석에 의해 29종의 다재내성 균주로 관찰 되었고, RAPD 분석에 의해 19종은 IPM-1 유전자형을, 2종은 VIM-2 유전자형을 만들었다. MBL 유전자 검출 시험을 통해 19종의 IMP-1 생성 녹농균 중에서 16종이 유사한 유전자형을 보였고, 3종은 다른 유전자형이 관찰되었다. 임상에서 분리한 IMP-1 생성 다재내성 녹농균의 비율은 꾸준히 증가하고 있다. 이번 연구는 2010년 국내 임상에서 분리한 녹농균의 항생제 다제내성 패턴과 유전자형에 대한 정보를 제공한다. Pseudomonas aeruginosa is an opportunistic Gram-negative bacterium that causes serious infection, particularly in immunocompromised patients. Also, P. aeruginosa possessing carbapenem-resistant metallo-${\beta}$-lactamases (MBL) has been reported with increasing frequency in Korea. We therefore analyzed the level of multidrug-resistant clinical P. aeruginosa isolated from a secondary hospital in Korea in 2010. A total of 92 isolates of P. aeruginosa were collected from Sahmyook Medical Center in 2010. Susceptibility to antimicrobial agents was determined by analysis of the minimum inhibitory concentration test; the inhibitor-potentiated disk diffusion (IPD) test was performed for MBL detection. RAPD-PCR was used for genotyping to rapidly characterize P. aeruginosa strains isolated from clinical patients. The percentages of non-susceptible isolates were as follows: 40.2% to ceftazidime, 58.7% to meropenem, 56.5% to gentamicin, 46.7% to tobramycin, 62.0% to ciprofloxacin and 97.8% to chloramphenicol. The 29 multidrug-resistant strains were screened by the IPD test: of the 21 PCR-positive isolates, 19 were IPM-1 producers and 2 were VIM-2 producers. Among the 19 IMP-1-producing P. aeruginosa isolates, 16 isolates showed similar patterns, and three different banding patterns were observed. The proportion of IMP-1-producing multidrug-resistant P. aeruginosa from clinical isolates steadily increased in this secondary hospital in Korea in 2010. This study provides information about the antimicrobial-resistant patterns and genotype of multidrug-resistant P. aeruginosa isolated from clinical isolates in Korea, 2010.

        • KCI등재

          Cellulose degrading basidiomycetes yeast isolated from the gut of grasshopper in Korea

          김주영,장준휘,박지현,정희영,박종석,조성진,이훈복,성기호,김명겸,Kim, Ju-Young,Jang, Jun Hwee,Park, Ji-Hyun,Jung, Hee-Young,Park, Jong-Seok,Cho, Sung-Jin,Lee, Hoon Bok,Limtong, Savitree,Subramani, Gayathri,Sung, Gi-Ho,Kim, Myung The Microbiological Society of Korea 2018 미생물학회지 Vol.54 No.4

          메뚜기는 광합성으로 고정된 탄소의 소화에서 중요한 역할을 한다. 장내 미생물 군의 도움으로, 메뚜기는 셀룰로오스 및 헤미셀룰로오스와 같은 잎의 성분을 분해할 수 있다. 본 연구는 한국의 기도에서 수집한 메뚜기 껍질에서 추출한 셀룰로오스 분해 효모 균주를 조사하기 위해 이루어졌다. 효모 균주 중 ON2와 ON17 (두 균주)과 ON6 (한 균주)는 CMC-플레이트 분석에서 셀룰로오스 활성을 보였다. Large subunit rDNA의 D1/D2영역의 서열과 internal transcribed spacer (ITS) 영역의 분석 결과, ON2와 ON17 균주가 Papiliotrema aspenensis CBS $13867^T$와 가장 밀접하게 관련되어 있었고(D1/D2 영역의 서열 유사성은 100% ITS에서 99.4%의 서열 유사성) ON6 균주는 Saitozyma flava와 관련된(D1/D2영역에서 100%, ITS에서 99.0%) 밀접하게 관련이 있었다. 이 세 가지 효모 균주는 모두 셀룰로오스를 분해할 수 있으므로 공생하는 효모들은 탄수화물 분해를 위한 효소를 자체적으로 생산하고 당 단당체를 휘발성 지방산으로 전환시킬 수 있다. Tremellomycetes에 속하는 공생 효모 균주인 ON2, ON17 (Papilioterma 속)과 ON6 (Saitozyma속)은 한국에는 보고되지 않은 균주이다. Grasshoppers play vital role in the digestion of photosynthetically fixed carbons. With the aid of intestinal microflora, the grasshopper can degrade leaves constituents such as cellulose and hemicellulose. The purpose of this study was to examine cellulolytic yeast isolates from the gut of grasshoppers collected in Gyeonggi Province, South Korea. Among the yeast isolates, ON2, ON17 (two strains), and ON6 (one strain) showed positive cellulolytic activity in the CMC-plate assay. The sequence analyses of D1/D2 domains of the large subunit rDNA gene and the internal transcribed spacer (ITS) regions revealed that the strains ON2 and ON17 were most closely related to Papiliotrema aspenensis CBS $13867^T$ (100%, sequence similarity in D1/D2 domains; 99.4% sequence similarity in ITS) and strain ON6 related to Saitozyma flava (100% in D1/D2 domains; 99.0% in ITS). All these three yeast strains are capable of degrading cellulose; therefore, the members of endosymbiotic yeasts may produce their own enzymes for carbohydrate degradation and convert mobilized sugar monomers to volatile fatty acids. Thus, the endosymbiotic yeast strains ON2, ON17 (represents the genus Papilioterma) and ON6 (Saitozyma) belonging to the family Tremellomycetes, are unreported strains in Korea.


          Outbreaks of Imipenem-Resistant Acinetobacter baumannii Producing Carbapenemases in Korea

          Jeong Seok-Hoon,Bae Il-Kwon,Park Kwang-Ok,An Young-Jun,Sohn Seung-Ghyu,Jang Seon-Ju,Sung Kwang-Hoon,Yang Ki-Suk,Lee Kyung-Won,Young Dong-Eun,Lee Sang-Hee The Microbiological Society of Korea 2006 The journal of microbiology Vol.44 No.4

          Among 53 Acinetobacter baumannii isolates collected in 2004, nine imipenem-resistant isolates were obtained from clinical specimens taken from patients hospitalized in Busan, Korea. Nine carbapenemase-producing isolates were further investigated in order to determine the mechanisms underlying resistance. These isolates were then analyzed via antibiotic susceptibility testing, microbiological tests of carbapenemase activity, pI determination, transconjugation test, enterobacterial repetitive consensus (ERIC)-PCR, and DNA sequencing. One outbreak involved seven cases of infection by A. baumannii producing OXA-23 ${\beta}-lactamase$, and was found to have been caused by a single ERIC-PCR clone. During the study period, the other outbreak involved two cases of infection by A. baumannii producing IMP-1 ${\beta}-lactamase$. The two clones, one from each of the outbreaks, were characterized via a modified cloverleaf synergy test and an EDTA-disk synergy test. The isoelectric focusing of the crude bacterial extracts detected nitrocefin-positive bands with pI values of 6.65 (OXA-23) and 9.0 (IMP-1). The PCR amplification and characterization of the amplicons via direct sequencing showed that the clonal isolates harbored $bla_{IMP-1}$ or $bla_{oxA-23}$ determinants. The two clones were characterized by a multidrug resistance phenotype that remained unaltered throughout the outbreak. This resistance encompassed penicillins, extended-spectrum cephalosporins, carbapenems, monobactams, and aminoglycosides. These results appear to show that the imipenem resistance observed among nine Korean A. baumannii isolates could be attributed to the spread of an IMP-lor OXA-23-producing clone. Our microbiological test of carbapenemase activity is a simple method for the screening of clinical isolates producing class D carbapenemase and/or class B $metallo-{\beta}-lactamase$, in order both to determine their clinical impact and to prevent further spread.

        • KCI등재

          Colistin resistance in Enterobacter spp. isolates in Korea

          Hong, Yoon-Kyoung,Lee, Ji-Young,Ko, Kwan Soo MICROBIOLOGICAL SOCIETY OF KOREA 2018 JOURNAL OF MICROBIOLOGY -SEOUL- Vol.56 No.6

          We investigated the colistin resistance rate among 356 Enterobacter spp. clinical isolates from eight hospitals in Korea. Antibiotic susceptibility testing was performed by broth microdilution. While 51 of 213 (23.9%) Enterobacter cloacae isolates were colistin-resistant, only six of 143 (4.2%) E. aerogenes isolates showed resistance. We also identified the skip well phenotype in eight E. cloacae and three E. aerogenes isolates. Multilocus sequence typing for E. cloacae and randomly amplified polymorphic DNA analysis and enterobacterial repetitive intergenic consensus PCR for E. aerogenes revealed that clonal spreading of colistin-resistant and skip well Enterobacter spp. isolates had not occurred. In vitro time-kill assays were performed with three colistin-resistant, three skip well, and two colistin-susceptible isolates of E. cloacae and E. aerogenes. Inconsistent results were observed among isolates with skip well phenotypes; while some were eradicated by 2 mg/L colistin, others were not. This suggests that skip well isolates have differentiated into different categories. As the high rates of colistin resistance in E. cloacae detected are of clinical concern, continuous monitoring is warranted. In addition, the clinical implications and mechanisms of the skip well phenotype should be investigated to ensure the appropriate use of colistin against Enterobacter infections.

        맨 위로 스크롤 이동