Influence of water-stressed rice on feeding behavior of brown planthopper, Nilaparvata lugens (Stål)

Ye Tan,Mufei Zhu,Wenyan Xu,Wenwu Zhou,Dongdong Lu,Hanwu Shang,Zengrong Zhu 한국응용곤충학회 2017 Journal of Asia-Pacific Entomology Vol.20 No.2

Climate change can impact insects through abnormal weather conditions such as elevated temperatures and droughts. Crops experiencing periods of water stress from droughts may have significant effects on pest populations because of physiology changes, insect behavior and adaptation. Herewe report on the effects ofwater stress on the rice pest, the brown planthopper (BPH), Nilaparvata lugens (Stål). In themultiple choice test, both the fifth instar nymphs and female adults BPH preferred plants under the low stress level over control and high stress plants. The electrical penetration graph (EPG) showed that the fifth instar nymphs spent more time in non-penetration activities while the female adults spent less. Both the fifth instar nymphs and female adults spent more time in salivation on high stress plants than in control and low stress plants indicating difficulties in feeding. In addition there was marked increase in probe numbers made by both the fifth instar nymphs and the female adults in the feeding duration. The fifth instar nymphs made twice as many numbers of probes than those in the control (9.36 vs to 5.25 min) while the female adults made about 4 times more probes (13.13 vs 3.00 min). Both the fifth instar nymphs and the female adults producedmore honeydewon the lowstress plants suggesting that plants in low water stress favored insect feeding. These findings indicate that BPH in low water stress rice plants seem to do better which supports the theory that drought induces pest outbreaks.

Ginsenoside Rg1 promotes neurite growth of retinal ganglion cells through cAMP/PKA/CREB pathways

Ye-ying Jiang,Rong-yun Wei,Kai Tang,Zhen Wang,Ning-hua Tan The Korean Society of Ginseng 2024 Journal of Ginseng Research Vol.48 No.2

Background: Mechanisms of synaptic plasticity in retinal ganglion cells (RGCs) are complex and the current knowledge cannot explain. Growth and regeneration of dendrites together with synaptic formation are the most important parameters for evaluating the cellular protective effects of various molecules. The effect of ginsenoside Rg1 (Rg1) on the growth of retinal ganglion cell processes has been poorly understood. Therefore, we investigated the effect of ginsenoside Rg1 on the neurite growth of RGCs. Methods: Expression of proteins and mRNA were detected by Western blot and qPCR. cAMP levels were determined by ELISA. In vivo effects of Rg1 on RGCs were evaluated by hematoxylin and eosin, and immunohistochemistry staining. Results: This study found that Rg1 promoted the growth and synaptic plasticity of RGCs neurite by activating the cAMP/PKA/CREB pathways. Meanwhile, Rg1 upregulated the expression of GAP43, Rac1 and PAX6, which are closely related to the growth of neurons. Meantime, H89, an antagonist of PKA, could block this effect of Rg1. In addition, we preliminarily explored the effect of Rg1 on enhancing the glycolysis of RGCs, which could be one of the mechanisms for its neuroprotective effects. Conclusion: Rg1 promoted neurite growth of RGCs through cAMP/PKA/CREB pathways. This study may lay a foundation for its clinical use of optic nerve diseases in the future.

Influence of Ribosomal Protein L39-L in the Drug Resistance Mechanisms of Lacrimal Gland Adenoid Cystic Carcinoma Cells

Ye, Qing,Ding, Shao-Feng,Wang, Zhi-An,Feng, Jie,Tan, Wen-Bin Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.12

Background: Cancer constitutes a key pressure on public health regardless of the economy state in different countries. As a kind of highly malignant epithelial tumor, lacrimal gland adenoid cystic carcinoma can occur in any part of the body, such as salivary gland, submandibular gland, trachea, lung, breast, skin and lacrimal gland. Chemotherapy is one of the key treatment techniques, but drug resistance, especially MDR, seriously blunts its effects. As an element of the 60S large ribosomal subunit, the ribosomal protein L39-L gene appears to be documented specifically in the human testis and many human cancer samples of different origins. Materials and Methods: Total RNA of cultured drug-resistant and susceptible lacrimal gland adenoid cystic carcinoma cells was seperated, and real time quantitative RT-PCR were used to reveal transcription differences between amycin resistant and susceptible strains of lacrimal gland adenoid cystic carcinoma cells. Viability assays were used to present the amycin resistance difference in a RPL39-L transfected lacrimal gland adenoid cystic carcinoma cell line as compared to control vector and null-transfected lacrimal gland adenoid cystic carcinoma cell lines. Results: The ribosomal protein L39-L transcription level was 6.5-fold higher in the drug-resistant human lacrimal gland adenoid cystic carcinoma cell line than in the susceptible cell line by quantitative RT-PCR analysis. The ribosomal protein L39-L transfected cells revealed enhanced drug resistance compared to plasmid vector-transfected or null-transfected cells as determined by methyl tritiated thymidine (3H-TdR) incorporation. Conclusions: The ribosomal protein L39-L gene could possibly have influence on the drug resistance mechanism of lacrimal gland adenoid cystic carcinoma cells.

DATCN: Deep Attention fused Temporal Convolution Network for the prediction of monitoring indicators in the tunnel

Junchen Ye,Bowen Du,Zhixin Zhang,Xuyan Tan,Wentao Li,Weizhong Chen 국제구조공학회 2022 Smart Structures and Systems, An International Jou Vol.30 No.6

The prediction of structural mechanical behaviors is vital important to early perceive the abnormal conditions and avoid the occurrence of disasters. Especially for underground engineering, complex geological conditions make the structure more prone to disasters. Aiming at solving the problems existing in previous studies, such as incomplete consideration factors and can only predict the continuous performance, the deep attention fused temporal convolution network (DATCN) is proposed in this paper to predict the spatial mechanical behaviors of structure, which integrates both the temporal effect and spatial effect and realize the cross-time prediction. The temporal convolution network (TCN) and self-attention mechanism are employed to learn the temporal correlation of each monitoring point and the spatial correlation among different points, respectively. Then, the predicted result obtained from DATCN is compared with that obtained from some classical baselines, including SVR, LR, MLP, and RNNs. Also, the parameters involved in DATCN are discussed to optimize the prediction ability. The prediction result demonstrates that the proposed DATCN model outperforms the state-of-the-art baselines. The prediction accuracy of DATCN model after 24 hours reaches 90 percent. Also, the performance in last 14 hours plays a domain role to predict the short-term behaviors of the structure. As a study case, the proposed model is applied in an underwater shield tunnel to predict the stress variation of concrete segments in space.

Bimetal Catalysts for Efficient Electrochemical Carbon Dioxide Conversion

Daniel Tan,Ye Eun Jeon(전예은),Wonhee Lee(이원희),Young Eun Kim(김영은),You Na Ko(고유나),Ki Tae Park(박기태) 한국에너지기후변화학회 2022 한국에너지기후변화학회 학술대회 Vol.2022 No.10

White-Matter Hyperintensities and Lacunar Infarcts Are Associated with an Increased Risk of Alzheimer’s Disease in the Elderly in China

Shuai Ye,Shuyang Dong,Jun Tan,Le Chen,Hai Yang,Yang Chen,Zeyan Peng,Yingchao Huo,Juan Liu,Mingshan Tang,Yafei Li,Huadong Zhou,Yong Tao 대한신경과학회 2019 Journal of Clinical Neurology Vol.15 No.1

Background and Purpose This study investigated the contribution of white-matter hyperintensities (WMH) and lacunar infarcts (LI) to the risk of Alzheimer’s disease (AD) in an elderly cohort in China. Methods Older adults who were initially cognitively normal were examined with MRI at baseline, and followed for 5 years. WMH were classified as mild, moderate, or severe, and LI were classified into a few LI (1 to 3) or many LI (≥4). Cognitive function was assessed using the Mini Mental State Examination and the Activities of Daily Living scale. Results Among the 2,626 subjects, 357 developed AD by the end of the 5-year follow-up period. After adjusting for age and other potential confounders, having only WMH, having only LI, and having both WMH and LI were associated with an increased risk of developing AD compared with having neither WMH nor LI. Moderate and severe WMH were associated with an increased risk of developing AD compared with no WMH. Furthermore, patients with many LI had an increased risk of developing AD compared with no LI. Conclusions Having moderate or severe WMH and many LI were associated with an increased risk of developing AD, with this being particularly striking when both WMH and LI were present.

MED1 induces M2 polarization of tumor-associated macrophages to aggravate breast cancer

Shen Ye,Zhou Lianming,Xu Meiyu,Tan Zhanhai,Yao Kai,Wang Wenjie 한국유전학회 2023 Genes & Genomics Vol.45 No.12

Background Breast cancer is a common malignant tumor in female, and its 5-year survival rate remains low. The correlation between mediator subunit 1 (MED1) gene and macrophage phenotypic transformation may be a key factor affecting the therapeutic effect on cancer. Objective The present study intended to explore the role of MED1 in macrophage polarization and its further influence on the malignant behaviors of breast cancer. Methods Bioinformatics analysis was carried out to predict the expression pattern of MED1 in breast cancer. Flow cytometry was conducted to detect the effect of MED1 overexpression or silencing on macrophage polarization. ELISA was applied to analyze the effect of abnormal MED1 expression on cytokine secretion of macrophages. CCK-8, colony formation, Transwell and scratch healing assays were applied to investigate the effects of macrophage conditioned medium on the malignant behaviors of breast cancer cells. Results MED1 expression was prominently increased in M2 macrophages, and overexpression of MED1 significantly increased M2 polarization of tumor-associated macrophages (TAMs) and IL-10 cytokine level. Meanwhile, M2 macrophages with MED1 overexpression could significantly promote the malignant behaviors of breast cancer cells. Dasatinib rescue experiment further confirmed that MED1-induced M2 macrophage polarization could facilitate the malignant progression of breast cancer cells. Conclusion In summary, MED1 could induce M2 macrophage polarization and thus regulate the malignant behaviors of breast cancer cells.

TRIM29 Overexpression Promotes Proliferation and Survival of Bladder Cancer Cells through NF-κB Signaling

Shu-Tao Tan,Sheng-Ye Liu,Bin Wu 대한암학회 2016 Cancer Research and Treatment Vol.48 No.4

Purpose TRIM29 overexpression has been reported in several human malignancies and showed correlation with cancer cell malignancy. The aim of the current study is to examine its clinical significance and biological roles in human bladder cancer tissues and cell lines. Materials and Methods A total of 102 cases of bladder cancer tissues were examined for TRIM29 expression by immunohistochemistry. siRNA and plasmid transfection were performed in 5637 and BIU- 87 cell lines. Cell Counting Kit-8, flow cytometry, western blot, and real-time polymerase chain reaction were performed to examine its biological roles and mechanism in bladder cancer cells. Results We found that TRIM29 overexpression showed correlation with invading depth (p=0.0087). Knockdown of TRIM29 expression in bladder cancer cell line 5637 inhibited cell growth rate and cell cycle transition while its overexpression in BIU-87 cells accelerated cell proliferation and cell cycle progression. TRIM29 overexpression also inhibited cell apoptosis induced by cisplatin. In addition, we demonstrated that TRIM29 depletion decreased while its overexpression led to upregulated expression of cyclin D1, cyclin E, and Bcl-2. We also showed that TRIM29 knockdown inhibited protein kinase C (PKC) and nuclear factor B (NF-B) signaling while its overexpression stimulated the PKC and NF-B pathways. BAY 11-7082 (NF-B inhibitor) partly attenuated the effect of TRIM29 on expression of cyclin and Bcl-2. Treatment with PKC inhibitor staurosporine resulted in ameliorated TRIM29 induced activation of NF-B. Conclusion The current study demonstrated that TRIM29 upregulates cyclin and Bcl family proteins level to facilitate malignant cell growth and inhibit drug-induced apoptosis in bladder cancer, possibly through PKC–NF-B signaling pathways.

Multifunctional poly(2,5-benzimidazole)/carbon nanotube composite films

Kang, Ji-Ye,Eo, Soo-Mi,Jeon, In-Yup,Choi, Yeong Suk,Tan, Loon-Seng,Baek, Jong-Beom Wiley Subscription Services, Inc., A Wiley Company 2010 Journal of polymer science Part A, Polymer chemist Vol.48 No.5

<P>The AB-monomer, 3,4-diaminobenzoic acid dihydrochloride, was recrystallized from an aqueous hydrochloric acid solution and used to synthesize high-molecular-weight poly(2,5-benzimidazole) (ABPBI). ABPBI/carbon nanotube (CNT) composites were prepared via in situ polymerization of the AB-monomer in the presence of single-walled carbon nanotube (SWCNT) or multiwalled carbon nanotube (MWCNT) in a mildly acidic polyphosphoric acid. The ABPBI/SWCNT and ABPBI/MWCNT composites displayed good solubility in methanesulfonic acid and thus, uniform films could be cast. The morphology of these composite films was studied by X-ray diffraction, scanning electron microscopy, transmission electron microscopy, and atomic force microscopy. The results showed that both types of CNTs were uniformly dispersed into the ABPBI matrix. Tensile properties of the composite films were significantly improved when compared with ABPBI, and their toughness (∼200 MPa) was close to the nature's toughest spider silk (∼215 MPa). The electrical conductivities of ABPBI/SWCNT and ABPBI/MWCNT composite films were 9.10 × 10<SUP>−5</SUP> and 2.53 × 10<SUP>−1</SUP> S/cm, respectively, whereas that of ABPBI film was 4.81 × 10<SUP>−6</SUP> S/cm. These values are ∼19 and 52,700 times enhanced by the presence of SWCNT and MWCNT, respectively. Finally, without acid impregnation, the ABPBI film was nonconducting while the SWCNT- and MWCNT-based composites were proton conducting with maximum conductivities of 0.018 and 0.017 S/cm, respectively. © 2010 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem 48: 1067–1078, 2010</P> <B>Graphic Abstract</B> <P>Multifunctional poly(2,5-benzimidazole) (ABPBI)/carbon nanotube (CNT) composites were prepared via in situ polymerization of 3,4-diaminobenzoic acid dihydrochloride in the presence of single-walled carbon nanotube (SWCNT) or multiwalled carbon nanotube (MWCNT) in a mildly acidic polyphosphoric acid (PPA). The resultant coagulated ABPBI/SWCNT and ABPBI/MWCNT composite films displayed outstanding physical properties with toughness of ∼200 MPa, a value that was close to the nature's toughest spider silk (215 MPa). They showed electrical conductivity of 9.10 × 10<SUP>−5</SUP> and 2.53 × 10<SUP>−1</SUP> S/cm, respectively, 1–4 orders of magnitudes higher than that of ABPBI film. Without acid doping, the composite films were also proton conducting with maximum conductivities of 0.017–0.018 S/cm. <img src='wiley_img/0887624X-2010-48-5-POLA23862-gra001.gif' alt='wiley_img/0887624X-2010-48-5-POLA23862-gra001'> </P>

Increasing Demeclocycline Production in Streptomyces aureofaciens by Manipulating the Expression of a Novel SARP Family Regulator and Its Genes

Yan-Ying Tan,Guang-Yao Zhu,Rui-Fang Ye,Hong-Zhou Zhang,De-Yu Zhu 한국생물공학회 2021 Biotechnology and Bioprocess Engineering Vol.26 No.6

Demeclocycline (DMCTC), a tetracycline derivative antibiotic produced by Streptomyces aureofaciens, has attracted attention owing to its high bioavailability, prolonged maintenance of a therapeutic concentration, and greater efficacy against many infectious microorganisms. However, the productivity of the DMCTC-producing strains has remained low. Thus, it is necessary to identify gene-knockout or amplification targets to increase DMCTC production. Here, we demonstrated that ctcB, which encodes a Streptomyces antibiotic regulatory protein (SARP), and ctcC, which encodes a resistance gene, positively regulate the biosynthesis of DMCTC in S. aureofaciens strain DT1. In particular, overexpression of the ctcB gene in S. aureofaciens DT1 significantly enhanced DMCTC production, resulting in increased expression of ctcG, ctcN, ctcQ, ctcH, ctcV, and ctcC. The deletion of ctcB dramatically reduced the DMCTC level, implying that CtcB is an activator of DMCTC biosynthesis. Although overexpression of the ctcC, which encodes a ribosomal protection protein, enhancing DMCTC biosynthesis in S. aureofaciens DT1, the improvement was limited compared with that achieved by ctcB overexpression. This is the first study to identify the role of ctcB and ctcC in DMCTC accumulation; these genes may also be ideal candidate targets for facilitating DMCTC production by other Streptomyces strains.