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Pathological Impact on the Phyllosphere Microbiota of Artemisia argyi by Haze
( Yu-zhu Zhang ),( De-yu Jiang ),( Chi Zhang ),( Kun Yang ),( Huai-fu Wang ),( Xiu-wen Xia ),( Wei-jun Ding ) 한국미생물생명공학회(구 한국산업미생물학회) 2021 Journal of microbiology and biotechnology Vol.31 No.4
The pathological impact of haze upon the phyllosphere microbiota awaits investigation. A moderate degree of haze environment and a clean control were selected in Chengdu, China. Artemisia argyi, a ubiquitously distributed and extensively applied Chinese herb, was also chosen for experiment. Total genome DNA was extracted from leaf samples, and for metagenome sequencing, an Illumina HiSeq 2500 platform was applied. The results showed that the gene numbers of phyllosphere microbiota derived from haze leaves were lower than those of the clean control. The phyllosphere microbiota derived from both haze and clean groups shared the same top ten phyla; the abundances of Proteobacteria, Actinomycetes and Anorthococcuso of the haze group were substantially increased, while Ascomycetes and Basidiomycetes decreased. At the genus level, the abundances of Nocardia, Paracoccus, Marmoricola and Knoelia from haze leaves were markedly increased, while the yeasts were statistically decreased. KEGG retrieval demonstrated that the functional genes were most annotated to metabolism. An interesting find of this work is that the phyllosphere microbiota responsible for the synthesis of primary and secondary metabolites in A. argyi were significantly increased under a haze environment. Relatively enriched genes annotated by eggNOG belong to replication, recombination and repair, and genes classified into the glycoside hydrolase and glycosyltransferase enzymes were significantly increased. In summary, we found that both structure and function of phyllosphere microbiota are globally impacted by haze, while primary and secondary metabolites responsible for haze tolerance were considerably increased. These results suggest an adaptive strategy of plants for tolerating and confronting haze damage.
Damage detection of subway tunnel lining through statistical pattern recognition
Yu, Hong,Zhu, Hong P.,Weng, Shun,Gao, Fei,Luo, Hui,Ai, De M. Techno-Press 2018 Structural monitoring and maintenance Vol.5 No.2
Subway tunnel structure has been rapidly developed in many cities for its strong transport capacity. The model-based damage detection of subway tunnel structure is usually difficult due to the complex modeling of soil-structure interaction, the indetermination of boundary and so on. This paper proposes a new data-based method for the damage detection of subway tunnel structure. The root mean square acceleration and cross correlation function are used to derive a statistical pattern recognition algorithm for damage detection. A damage sensitive feature is proposed based on the root mean square deviations of the cross correlation functions. X-bar control charts are utilized to monitor the variation of the damage sensitive features before and after damage. The proposed algorithm is validated by the experiment of a full-scale two-rings subway tunnel lining, and damages are simulated by loosening the connection bolts of the rings. The results verify that root mean square deviation is sensitive to bolt loosening in the tunnel lining and X-bar control charts are feasible to be used in damage detection. The proposed data-based damage detection method is applicable to the online structural health monitoring system of subway tunnel lining.
Rice Production, Consumption and Trading in China (초)
( De Feng Zhu ),( Yu Ping Zhang ),( Jing Xiang ) 한국식품저장유통학회 ( 구 한국농산물저장유통학회 ) 2010 한국식품저장유통학회 학술대회 Vol.2010 No.-
China is the largest rice production country in the world. Chinese rice planting stands for 18.7 % of world rice planting area and contributes 28.5% of world rice production. Rice is a staple food in China. Its planting area and production stands for 27% and 38% of grain crops in China respectively. About 60% of the Chinese population lives on rice. Due to the decreasing rice area in the south, percentage of rice production in national grain production is decreasing in recent decades. Rice planting method is shifting from hand transplanting to labor saving planting methods, including seedling throwing, direct seeding and machine transplanting. Hybrid rice covers more than 55% of rice area. Average rice consumption per capita is 65.9 kg in the northeast and 120.9 in the south in the period from 2003 to 2009. In recent years, rice consumption per capita is decreasing by 3.3% in the northeast and 2.8% in the south annually. On the whole, the rice production in China will be sufficient to meet the consumption demand of the population in urban and rural areas. But there still exists a certain gap between the current production and domestic potential demand of quality rice in China, which will provide a great potential for rice trade. China imports and exports rice. Annual import rice is about 0.41 million ton and export 1.27million ton during the period from 1980 to 2009. Rice variety of import main is good quality rice, such as aromatic rice, while that of export main is Japonica and normal indica rice.
De-Hai Li,Tian-Jiao Zhu,Hong-Bing Liu,Yu-Chun Fang,Qian-Qun Gu,Wei-Ming Zhu 대한약학회 2006 Archives of Pharmacal Research Vol.29 No.8
Four known butenolides were isolated from the ethyl acetate extracts of the culture broth of the marine-derived bacterium, Streptoverticillium luteoverticillatum, by bioassay-guided fractionation. The structures were identified on the basis of spectral data. The absolute configuration of compound (1) was determined by CD spectrum for the first time. Compounds 1-4 showed in vitro cytotoxicity against the murine lymphoma P388 and human leukemia K562 cell lines. This is the first report on the isolation of butenolides from the marine bacterium, Streptoverticillium luteoverticillatum, and their cytotoxic activities.
Two New Metabolites with Cytotoxicities from Deep-Sea Fungus, Aspergillus sydowi YH11-2
De-Hai Li,Sheng-Xin Cai,Li Tian,Zhen-Jian Lin,Tian-Jiao Zhu,Yu-Chun Fang,Pei-Pei Liu,Qian-Qun Gu,Wei-Ming Zhu 대한약학회 2007 Archives of Pharmacal Research Vol.30 No.9
Two new compounds, 2, 3, 5-trimethyl-6-(3-oxobutan-2-yl)-4H-pyran-4-one (1) and (2R)-2, 3- dihydro-7-hydroxy-6, 8-dimethyl-2-[(E)-prop-1-enyl] chromen-4-one (2), together with six known compounds (3-8), were isolated from a deep-sea fungus, identified as Aspergillus sydowi, by a bioassay-guided method. Their structures were elucidated by spectroscopic methods and the cytotoxicities were evaluated by SRB method.
Two New Metabolites with Cytotoxicities from Deep-Sea Fungus, Aspergillus sydowi YH11-2
Li, De-Hai,Cai, Sheng-Xin,Tian, Li,Lin, Zhen-Jian,Zhu, Tian-Jiao,Fang, Yu-Chun,Liu, Pei-Pei,Zhu, Wei-Ming,Gu, Qian-Qun 대한약학회 2007 Archives of Pharmacal Research Vol.30 No.9
Two new compounds, 2, 3, 5-trimethyl-6-(3-oxobutan-2-yl)-4H-pyran-4-one (1) and (2R)-2, 3-dihydro-7-hydroxy-6, 8-dimethyl-2-[(E)-prop-1-enyl] chromen-4-one (2), together with six known compounds (3-8), were isolated from a deep-sea fungus, identified as Aspergillus sydowi, by a bioassay-guided method. Their structures were elucidated by spectroscopic methods and the cytotoxicities were evaluated by SRB method.
Li, De-Hai,Zhu, Tian-Jiao,Liu, Hong-Bing,Fang, Yu-Chun,Gu, Qian-Qun,Zhu, Wei-Ming The Pharmaceutical Society of Korea 2006 Archives of Pharmacal Research Vol.29 No.8
Four known butenolides were isolated from the ethyl acetate extracts of the culture broth of the marine-derived bacterium, Streptoverticillium luteoverticillatum, by bioassay-guided fractionation. The structures were identified on the basis of spectral data. The absolute configuration of compound (1) was determined by CD spectrum for the first time. Compounds 1-4 showed in vitro cytotoxicity against the murine lymphoma P388 and human leukemia K562 cell lines. This is the first report on the isolation of butenolides from the marine bacterium, Streptoverticillium luteoverticillatum, and their cytotoxic activities.
An Experimental Model for Induction of Lung Cancer in Rats by Chlamydia Pneumoniae
Chu, De-Jie,Guo, Shui-Gen,Pan, Chun-Feng,Wang, Jing,Du, Yong,Lu, Xu-Feng,Yu, Zhu-Yuan Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.6
Objective: To assess induction effects of Chlamydia pneumoniae (Cpn) on lung cancer in rats. Methods: A lung cancer animal model was developed through repeated intratracheal injection of Cpn (TW-183) into the lungs of rats, with or without exposure to benzo(a)pyrene (Bp). Cpn antibodies (Cpn-IgA, -IgG, and -IgM) in serum were measured by microimmunofluorescence. Cpn-DNA or Cpn-Ag of rat lung cancer was detected through polymerase chain reaction or enzyme-linked immunosorbent assay. Results: The prevalence of Cpn infection was 72.9% (35/48) in the Cpn group and 76.7% (33/43) in the Cpn plus benzo(a)pyrene (Bp) group, with incidences of lung carcinomas in the two groups of 14.6% (7/48) and 44.2% (19/43), respectively (P-values 0.001 and <0.000 compared with normal controls). Conclusions: A rat model of lung carcinoma induced by Cpn infection was successfully established in the laboratory for future studies on the treatment, prevention, and mechanisms of the disease.
Formulation and Evaluation of Irinotecan Suppository for Rectal Administration
( Hai Yang Feng ),( Yu Ping Zhu ),( De Chuan Li ) 한국응용약물학회 2014 Biomolecules & Therapeutics(구 응용약물학회지) Vol.22 No.1
Irinotecan suppository was prepared using the moulding method with a homogeneous blend. A sensitive and specific fluorescence method was developed and validated for the determination of irinotecan in plasma using HPLC. The pharmacokinetics of intravenous administered and rectal administered in rabbits was investigated. Following a single intravenous dose of irinotecan (50 mg/kg), the plasma irinotecan concentration demonstrated a bi-exponential decay, with a rapid decline over 15 min. Cmax, t1/2, AUC0-30h and AUC0-∞ were 16.1 ± 2.7 g/ml, 7.6 ± 1.2 h, 71.3 ± 8.8 μg·h/μl and 82.3 ± 9.5 μg·h/ml, respectively. Following rectal administration of 100 mg/kg irinotecan, the plasma irinotecan concentration reached a peak of 5.3 ± 2.5 μg/ml at 4 h. The AUC0-30h and AUC0-∞ were 32.2 ± 6.2 μg·h/ml and 41.6 ± 7.2 μg·h/ml, respectively. It representing ~50.6% of the absolute bioavailability.