MiR-34b/c rs4938723 Polymorphism Significantly Decreases the Risk of Digestive Tract Cancer: Meta-analysis

Ji, Tian-Xing,Zhi, Cheng,Guo, Xue-Guang,Zhou, Qiang,Wang, Guo-Qiang,Chen, Bo,Ma, Fei-Fei Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.14

Background: Previous studies investigating the association between miR-34b/c rs4938723 polymorphism and cancer risk showed inconclusive. Here, we performed meta-analysis to investigate the association between miR- 34b/c rs4938723 polymorphism and digestive cancer risk. Materials and Methods: Literature database including PubMed, OVID, Chinese National Knowledge Infrastructure (CNKI) were searched for publications concerning the association between the miR-34b/c rs4938723 polymorphism and digestive cancer risk. Results: A total of 6 studies consisting of 3246 cases and 3568 controls were included in this meta-analysis. The combined analysis suggested the miR-34b/c rs4938723 polymorphism significantly reduced digestive cancer risk under allelic model, homogeneous co-dominant model and recessive model (C vs T: OR=0.88, 95%CI=0.82-0.95, p-value=0.001; CC vs TT: OR =0.67, 95%CI=0.57-0.80, p-value=0.000; CC vs TT/TC: OR=0.68, 95%CI=0.58-0.80, p-value=0.000). Q-test and I2 test revealed no significant heterogeneity in all genotype comparisons. The Begger's funnel plot and Egger's test did not show significant publication bias. Conclusions: The current evidence supports the conclusion that the miR-34b/c rs4938723 polymorphism decreases an individual's susceptibility to digestive cancers.

Identification of novel rheumatoid arthritis-associated MiRNA-204-5p from plasma exosomes

Wu Long-Fei,Zhang Qin,Mo Xing-Bo,Lin Jun,Wu Yang-Lin,Lu Xin,He Pei,Wu Jian,Guo Yu-Fan,Wang Ming-Jun,Ren Wen-Yan,Deng Hong-Wen,Lei Shu-Feng,Deng Fei-Yan 생화학분자생물학회 2022 Experimental and molecular medicine Vol.54 No.-

Rheumatoid arthritis (RA) is an autoimmune disease characterized by infiltration of immune cells in the synovium. However, the crosstalk of immune cells and synovial fibroblasts is still largely unknown. Here, global miRNA screening in plasma exosomes was carried out with a custom microarray (RA patients vs. healthy controls = 9:9). A total of 14 exosomal miRNAs were abnormally expressed in the RA patients. Then, downregulated expression of exosomal miR-204-5p was confirmed in both the replication (RA patients vs. healthy controls = 30:30) and validation groups (RA patients vs. healthy controls = 56:60). Similar to the findings obtained in humans, a decreased abundance of exosomal miR-204-5p was observed in mice with collagen-induced arthritis (CIA). Furthermore, Spearman correlation analysis indicated that plasma exosomal miR-204-5p expression was inversely correlated with disease parameters of RA patients, such as rheumatoid factor, erythrocyte sedimentation rate, and C-reactive protein. In vitro, our data showed that human T lymphocytes released exosomes containing large amounts of miR-204-5p, which can be transferred into synovial fibroblasts, inhibiting cell proliferation. Overexpression of miR-204-5p in synovial fibroblasts suppressed synovial fibroblast activation by targeting genes related to cell proliferation and invasion. In vivo assays found that administration of lentiviruses expressing miR-204-5p markedly alleviated the disease progression of the mice with CIA. Collectively, this study identified a novel RA-associated plasma exosomal miRNA-204-5p that mediates the communication between immune cells and synovial fibroblasts and can be used as a potential biomarker for RA diagnosis and treatment.

The Study of Pilot Process of Separation and Purification of Catechin Using Wood Fiber Resin

Xing-hai Zhang,Jin-wei Xu,Yue-fei Wang,Yin Gao,You-ying Tu 한국차학회 2015 한국차학회지 Vol.- No.S

This paper studies the pilot production of tea polyphenol (TP) from membrane filtered tea extract using wood fiber resin. We extract, isolate and purify TP using ultrasonic wave extraction, ultrafiltration membrane and wood fiber resin column chromatography techniques. We then use high performance liquid chromatography (HPLC) to filter optimal parameters and get the final product using spray drying method. The results show that after above process the content of caffeine, EGCg and catechin in 40% eluent is 0.607%, 60.7%, 86.95% separately and the low caffeine high catechin procuct yield rate is 7.0%. While in 10% eluent the content of caffeine, EGCg and catechin is 26.6%, 16.5%, 23.7% separately, and the high caffeine low catechin product yield rate is 7.3%. So the total summed yield rate is 14.3%. The pilot process may provide reference for future green TP production.

ZNF435, a Novel Human SCAN-containing Zinc Finger Protein, Inhibits AP-1-mediated Transcriptional Activation

Xing Gu,Mei Zheng,Xiangwei Fei,Zhenxing Yang,Fan Li,Chaoneng Ji,Yi Xie,Yumin Mao 한국분자세포생물학회 2007 Molecules and cells Vol.23 No.3

Identification and Characterization of Protein Encoded by orf382 as L-Threonine Dehydrogenase

( Fei Ma ),( Tian Wen Wang ),( Xing Yuan Ma ),( Ping Wang ) 한국미생물 · 생명공학회 2014 Journal of microbiology and biotechnology Vol.24 No.6

In the genome annotation of Escherichia coli MG1655, the orf382 (1,149 bp) is designated as a gene encoding an alcohol dehydrogenase that may be Fe-dependent. In this study, the gene was amplified from the genome by PCR and overexpressed in Escherichia coli BL21(DE3). The recombinant 6×His-tag protein was then purified and characterized. In an enzymatic assay using different hydroxyl-containing substrates (n-butanol, L-threonine, ethanol, isopropanol, glucose, glycerol, L-serine, lactic acid, citric acid, methanol, or D-threonine), the enzyme showed the highest activity on L-threonine. Characterization of the mutant constructed using gene knockout of the orf382 also implied the function of the enzyme in the metabolism of L-threonine into glycine. Considering the presence of tested substrates in living E. coli cel ls and previous literature, we believed that the suitable nomenclature for the enzyme should be an L-threonine dehydrogenase (LTDH). When using L-threonine as the substrate, the enzyme exhibited the best catalytic performance at 39°C and pH 9.8 with NAD+ as the cofactor. The determination of the Km values towards L-threonine (Km = 11.29 μM), ethanol (222.5 μM), and n-butanol (8.02 μM) also confirmed the enzyme as an LTDH. Furthermore, the LTDH was shown to be an ion-containing protein based on inductively coupled plasma-atomic emission spectrometry with an isoelectronic point of pH 5.4. Moreover, a circular dichroism analysis revealed that the metal ion was structurally and enzymatically essential, as its deprivation remarkably changed the α-helix percentage (from 12.6% to 6.3%).

Effect of O2 Plasma Treatment on Density-of-States in a-IGZO Thin Film Transistors

Xing-Wei Ding,Fei Huang,Sheng Li,Jianhua Zhang,Xue-Yin Jiang,Zhi-Lin Zhang 대한금속·재료학회 2017 ELECTRONIC MATERIALS LETTERS Vol.13 No.1

This work reports an efficient route for enhancing the performanceof amorphous InGaZnO (a-IGZO) thin film transistors (TFT). Themobility was greatly improved by about 38% by means of O2plasma treatment. Temperature-stress was carried out to investigatethe stability and extract the parameters related to activation energy(Ea) and density-of-states (DOS). The DOS was calculated on thebasis of the experimentally obtained Ea, which can explain theexperimental observation. A lower activation energy (Ea, ~0.72 eV)and a smaller DOS were obtained in the O2 plasma treatment TFTbased on the temperature-dependent transfer curves. The resultsshowed that temperature stability and electrical propertiesenhancements in a-IGZO thin film transistors were attributed to thesmaller DOS.

Law and Early Warning of Vertical Sluice Cluster Displacements in Soft Coastal Soil

Xing Yang,Cong Yuan,Miao Hou,Chengyang Zhou,Yan Ju,Fei Qi 대한토목학회 2023 KSCE Journal of Civil Engineering Vol.27 No.2

The vertical displacement, which is the product of natural sources and human activities, is the key factor affecting the sluice safety. This study provides a systematic approach used for analyzing the law and early warning of sluice cluster vertical displacements in coastal soft soil locations. Two important methods, including probability analysis and principal component analysis (PCA), are used to obtain the necessary information in this study. Among them, PCA is mainly used to identify the risk indices during vertical deformations of sluice cluster. As case studies, 27 sluices in a cluster in Northern Jiangsu Province's coastal area in China are chosen and 14 variables related to sluice uplift, settlement and differential settlement deformations are used. The PCA and additional evidence from the sluice deformation law are used to identify three variables as risk indices, including maximum differential settlement (MMDS), maximum cumulative vertical settlement (MCVS) and maximum cumulative vertical uplift (MCVU). This study divides the risk levels into five grades (i.e., Level 1 to Level 5) based on the selected risk indices and determines their risk thresholds based on the in-situ deformation data from 2010 to 2020. In general, the results demonstrate that the newly proposed approach exhibits an acceptable performance. However, the influence of epistemic and aleatory uncertainties on this study is worthy of further discussion in the future.

Characterization of the TAK1 gene in Apis cerana cerana (AccTAK1) and its involvement in the regulation of tissue-specific development

( Fei Meng ),( Ming Jiang Kang ),( Li Liu ),( Lu Luo ),( Bao Hua Xu ),( Xing Qi Guo ) 생화학분자생물학회(구 한국생화학분자생물학회) 2011 BMB Reports Vol.44 No.3

TGF-β activated kinase-1 (TAK1) plays a pivotal role in developmental processes in many species. Previous research has mainly focused on the function of TAK1 in model organisms, and little is known about the function of TAK1 in hymenoptera insects. Here, we isolated and characterized the TAK1 gene from Apis cerana cerana. Promoter analysis of AccTAK1 revealed the presence of transcription factor binding sites related to early development. Real-time quantitative PCR and immunohistochemistry experiments revealed that AccTAK1 was expressed at high levels in fourth instar larvae, primarily in the abdomen, in the intestinal wall cells of the midgut and in the secretory cells of the salivary glands. In addition, AccTAK1 expression in fourth instar larvae could be dramatically induced by treatment with pesticides and organic solvents. These observations suggest that AccTAK1 may be involved in the regulation of early development in the larval salivary gland and midgut. [BMB reports 2011; 44(3): 187-192]

Risk Factors, Patterns, and Outcomes of Late Recurrence after Liver Resection for Patients with Hepatocellular Carcinoma (Analysis of a Multicenter Cohort over 15 Years)

( Xin-fei Xu ),( Jiong-jie Yu ),( Ju-dong Li ),( Hao Xing ),( Jun Han ),( Zhen-li Li ),( Han Wu ),( Han Zhang ),( Jian-hong Zhong ),( Yi- Sheng Huang ),( Ya-hao Zhou ),( Ting-hao Chen ),( Hong Wang ) 대한간학회 2018 춘·추계 학술대회 (KASL) Vol.2018 No.1

Aims: Late recurrence (> 2 years) after liver resection of hepatocellular carcinoma (HCC) is usually considered as multi-centric tumors or de novo cancer formation. We aimed to investigate risk factors, patterns and outcomes of late recurrence after HCC resection. Methods: From a multicenter database from 2001 to 2015, 734 patients who were alive and recurrence-free at 2 years after curative resection of initial HCC were enrolled into this retrospective study. Univariate and multivariate Cox-regression analysis were used to identify independent risk factors of late recurrence. Patterns, treatments and outcomes of late recurrence were investigated and analyzed. Results: During a median follow-up of 78.0 months after surgery, 303 patients (41.3%) developed late recurrence. Multivariate analysis revealed that cirrhosis, macroscopic vascular invasion, satellites, and tumor size > 5cm were independent risk factors of late recurrence. Among them, 273 (90.1%) were sole intrahepatic recurrence, 30 (9.9%) were concurrent intrahepatic and extrahepatic recurrence, and none of them was sole extrahepatic recurrence; 165 (54.4%) patients received curative treatments for recurrent HCC, including re-resection, transplantation and local ablation. Multivariate analysis showed regular postoperative surveillance and receiving curative treatments were two independent protective factors of prolonging survival for those patients with late recurrence. Conclusions: Late recurrence is correlated with cirrhosis and certain tumor-related characteristics of initial HCC. The patterns of late recurrence suggest that postoperative surveillance after 2 years of surgery could be adjusted and more targeted. Regular postoperative surveillance improves the probability to receive curative treatments again, yielding to better outcomes for patients with late recurrence.

New Delay-range-dependent Exponential Estimates for Singular Systems with Time-varying Delay

Jin-Xing Lin,Xian-Lin Zhao,Shu-Min Fei 제어·로봇·시스템학회 2011 International Journal of Control, Automation, and Vol.9 No.2

This paper considers the problem of exponential stability for continuous-time singular systems with in-terval time-varying delay. By defining a novel Lyapunov-Krasovskii function and giving a tighter up-per bound of its derivative, a new delay-range-dependent exponential admissibility criterion, which not only guarantees the regularity, absence of impulses and exponential stability of the system but also gives the estimates of decay rate and decay coefficient, is established in terms of linear matrix inequality (LMI). The resulting criterion has advantages over the result previously reported by Haidar et al. [17] in that it involves fewer matrix variables but has less conservatism, which is established theoretically. Examples are provided to demonstrate the advantage of the proposed criterion.