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TAZ as a novel regulator of oxidative damage in decidualization via Nrf2/ARE/Foxo1 pathway
Yu Hai-Fan,Zheng Lian-Wen,Yang Zhan-Qing,Wang Yu-Si,Wang Ting-Ting,Yue Zhan-Peng,Guo Bin 생화학분자생물학회 2021 Experimental and molecular medicine Vol.53 No.-
TAZ, as a crucial effector of Hippo pathway, is required for spermatogenesis and fertilization, but little is known regarding its physiological function in uterine decidualization. In this study, we showed that TAZ was localized in the decidua, where it promoted stromal cell proliferation followed by accelerated G1/S phase transition via Ccnd3 and Cdk4 and induced the expression or activity of stromal differentiation markers Prl8a2, Prl3c1 and ALP, indicating the importance of TAZ in decidualization. Knockdown of TAZ impeded HB-EGF induction of stromal cell proliferation and differentiation. Under oxidative stress, TAZ protected stromal differentiation against oxidative damage by reducing intracellular ROS and enhancing cellular antioxidant capacity dependent on the Nrf2/ARE/Foxo1 pathway. TAZ strengthened the transcriptional activity of Nrf2 which directly bound to the antioxidant response element (ARE) of Foxo1 promoter region. Additionally, silencing TAZ caused accumulation of intracellular ROS through heightening NOX activity whose blockade by APO reversed the disruption in stromal differentiation. Further analysis revealed that TAZ might restore mitochondrial function, as indicated by the increase in ATP level, mtDNA copy number and mitochondrial membrane potential with the reduction in mitochondrial superoxide. Additionally, TAZ modulated the activities of mitochondrial respiratory chain complexes I and III whose suppression by ROT and AA resulted in the inability of TAZ to defend against oxidative damage to stromal differentiation. Moreover, TAZ prevented stromal cell apoptosis by upregulating Bcl2 expression and inhibiting Casp3 activity and Bax expression. In summary, TAZ might mediate HB-EGF function in uterine decidualization through Ccnd3 and ameliorate oxidative damage to stromal cell differentiation via Nrf2/ARE/Foxo1 pathway.
Cheng Xia,Rui-Tang Guo,Zhen-rui Zhang,Chen-yuan Fan,Yu-zhe Liu,Yu-cheng Lin,Chu-fan Li,Wei-Guo Pan 한국공업화학회 2023 Journal of Industrial and Engineering Chemistry Vol.128 No.-
Recently, the photocatalytic CO2 reduction technology is an effective solution to remit the energy crisis. Inorder to improve the photocatalytic performance, Z-scheme W18O49/NiAl-LDH composite catalysts wereprepared by hydrothermal method. Fortunately, the prepared catalysts revealed excellent photocatalyticperformance under the simulated sunlight, and CO and CH4 could be detected in the reduction products. WO/LDH-0.5 catalyst possessed the optimal activity, with CO and CH4 yield of 37.09 and 8.01 lmol g-1h1separately, which were 7.9 and 3.6 times that of NiAl-LDH monomer. In addition, W18O49 endowedW18O49/NiAl-LDH catalysts with photothermal effect, which raised the surface temperature andfacilitated the catalytic reaction. Meanwhile, the Z-scheme heterojunction composed of flower-likeNiAl-LDH and urchin-like W18O49 accelerated the separation of photoexcited carriers and enhanced theredox ability. Through a series of characterizations and investigations, this work is promising to breaknew ground for the design of photocatalysts with photothermal effect.
Yu, Wan-Guo,He, Yun,Chen, Yun-Fang,Gao, Xiao-Yao,Ning, Wan-E,Liu, Chun-You,Tang, Ting-Fan,Liu, Quan,Huang, Xiao-Cheng The Korean Society of Pharmacology 2019 The Korean Journal of Physiology & Pharmacology Vol.23 No.3
Fumigaclavine C (FC), an active indole alkaloid, is obtained from endophytic Aspergillus terreus (strain No. FC118) by the root of Rhizophora stylosa (Rhizophoraceae). This study is designed to evaluate whether FC has anti-adipogenic effects in 3T3-L1 adipocytes and whether it ameliorates lipid accumulation in high-fat diet (HFD)-induced obese mice. FC notably increased the levels of glycerol in the culture supernatants and markedly reduced lipid accumulation in 3T3-L1 adipocytes. FC differentially inhibited the expressions of adipogenesis-related genes, including the peroxisome proliferator-activated receptor proteins, CCAAT/enhancer-binding proteins, and sterol regulatory element-binding proteins. FC markedly reduced the expressions of lipid synthesis-related genes, such as the fatty acid binding protein, lipoprotein lipase, and fatty acid synthase. Furthermore, FC significantly increased the expressions of lipolysis-related genes, such as the hormone-sensitive lipase, Aquaporin-7, and adipose triglyceride lipase. In HFD-induced obese mice, intraperitoneal injections of FC decreased both the body weight and visceral adipose tissue weight. FC administration significantly reduced lipid accumulation. Moreover, FC could dose-dependently and differentially regulate the expressions of lipid metabolism-related transcription factors. All these data indicated that FC exhibited anti-obesity effects through modulating adipogenesis and lipolysis.
Guo-Wei Fan,Xue-Ying Lv,Ying Song,Huan-Yu Zhao,Liu Zhang 제어·로봇·시스템학회 2022 International Journal of Control, Automation, and Vol.20 No.8
Nonparallel ground track imaging of optical remote sensing satellite is an efficient imaging mode which could realize one-time transit imaging of complex observation tasks due to its fast and flexible imaging capability, and has attracted more attentions in the earth observation field recently. In this paper, an off-line attitude trajectory planning and on-line moving horizon attitude tracking control scheme based on optimal control idea is designed to satisfy the imaging requirement of high-performance attitude control oriented to nonparallel ground track imaging. Considering the entire satellite attitude adjustment of the drift angle, based on the geometric relationship of sapce vectors and the coordinate transformation principle, the three-axis attitude calculation of the precise pointing of key feature targets in the interested ground curve strip is conducted. Taking the attitude orientation as the constraint, the pseudospectral method is used following the established agile satellite control model to design the optimal trajectory of the attitude manever and the feedforward control torque of actuator for nonparallel ground track imaging. A moving horizon tracking control law based on the nonlinear error control model is designed to realize the highprecision attitude tracking control. The feasibility and effectiveness of the proposed control scheme are illustrated by numerical and imaging simulations.
Fan, Fang-Tian,Shen, Cun-Si,Tao, Li,Tian, Chao,Liu, Zhao-Guo,Zhu, Zhi-Jie,Liu, Yu-Ping,Pei, Chang-Song,Wu, Hong-Yan,Zhang, Lei,Wang, Ai-Yun,Zheng, Shi-Zhong,Huang, Shi-Le,Lu, Yin Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.5
Pyruvate kinase isozyme type M2 (PKM2) was first found in hepatocellular carcinoma (HCC), and its expression has been thought to correlate with prognosis. A large number of studies have demonstrated that epithelial-mesenchymal transition (EMT) is a crucial event in hepatocellular carcinoma (HCC) and associated metastasis, resulting in enhanced malignancy of HCC. However, the roles of PKM2 in HCC EMT and metastasis remain largely unknown. The present study aimed to determine the effects of PKM2 in EGF-induced HCC EMT and elucidate the molecular mechanisms in vitro. Our results showed that EGF promoted EMT in HCC cell lines as evidenced by altered morphology, expression of EMT-associated markers, and enhanced invasion capacity. Furthermore, the present study also revealed that nuclear translocation of PKM2, which is regulated by the ERK pathway, regulated ${\beta}$-catenin-TCF/LEF-1 transcriptional activity and associated EMT in HCC cell lines. These discoveries provide evidence of novel roles of PKM2 in the progression of HCC and potential therapeutic target for advanced cases.
Shear resistance of steel–concrete–steel deep beams with bidirectional webs
Yu-Tao Guo,Xin Nie,Jiansheng Fan,Mu-Xuan Tao 국제구조공학회 2022 Steel and Composite Structures, An International J Vol.42 No.3
Steel–concrete–steel composite structures with bidirectional webs (SCSBWs) are used in large-scale projects and exhibit good mechanical performances and constructional efficiency. The shear behaviors of SCSBW deep beam members in key joints or in locations subjected to concentrated forces are of concern in design. To address this issue, experimental program is investigated to examine the deep-beam shear behaviors of SCSBWs, in which the cracking process and force transfer mechanism are revealed. Compared with the previously proposed truss model, it is found that a strut-and-tie model is more suitable for describing the shear mechanism of SCSBW deep beams with a short span and sparse transverse webs. According to the experimental analyses, a new model is proposed to predict the shear capacities of SCSBW deep beams. This model uses strut-and-tie concept and introduces web shear and dowel action to consider the coupled multi mechanisms. A stress decomposition method is used to distinguish the contributions of different shear-transferring paths. Based on case studies, a simplified model is further developed, and the explicit solution is derived for design efficiency. The proposed models are verified using experimental data, which are proven to have good accuracy and efficiency and to be suitable for practical application.
Fan, M.,Yu, W.,Wang, W.,Guo, X.,Jin, K.,Miao, R.,Hou, W.,Kim, N.,Tao, J. Springer Science + Business Media 2017 JOURNAL OF MATERIALS ENGINEERING AND PERFORMANCE Vol.26 No.1
<P>Thin-multilayer Ti/Al laminates were prepared by one-step explosive bonding method to investigate the interface bonding and mechanical properties owing to their potential application in aerospace and auto industry. It was found that Ti/Al laminates prepared at the detonation velocity of 2100 m/s exhibited superior surface quality without any defects in comparison with other detonation velocities owing to the optimum explosive pressure and impact velocity. The interface morphologies and element distribution of Ti/Al laminates prepared at the detonation velocity of 2100 m/s were characterized by means of SEM and EDS. The results indicated that the linear and wavy bonding interface coexisted and element diffusion occurred in the interfacial zone. Furthermore, thin-multilayer Ti/Al laminates possessed high microhardness, excellent interlaminar shear and tensile properties.</P>
Guo, Hong-Yan,Xing, Yue,Sun, Yu-Qiao,Liu, Can,Xu, Qian,Shang, Fan-Fan,Zhang, Run-Hui,Jin, Xue-Jun,Chen, Fener,Lee, Jung Joon,Kang, Dongzhou,Shen, Qing-Kun,Quan, Zhe-Shan The Korean Society of Ginseng 2022 Journal of Ginseng Research Vol.46 No.6
Background: Ginseng possesses antitumor effects, and ginsenosides are considered to be one of its main active chemical components. Ginsenosides can further be hydrolyzed to generate secondary saponins, and 20(R)-panaxotriol is an important sapogenin of ginsenosides. We aimed to synthesize a new ginsengenin derivative from 20(R)-panaxotriol and investigate its antitumor activity in vivo and in vitro. Methods: Here, 20(R)-panaxotriol was selected as a precursor and was modified into its derivatives. The new products were characterized by <sup>1</sup>H-NMR, <sup>13</sup>C-NMR and HR-MS and evaluated by molecular docking, MTT, luciferase reporter assay, western blotting, immunofluorescent staining, colony formation assay, EdU labeling and immunofluorescence, apoptosis assay, cells migration assay, transwell assay and in vivo antitumor activity assay. Results: The derivative with the best antitumor activity was identified as 6,12-dihydroxy-4,4,8,10,14-pentamethyl-17-(2,6,6-trimethyltetrahydro-2H-pyran-2-yl)hexadecahydro-1H-cyclopenta[a]phenanthren-3-yl(tert-butoxycarbonyl)glycinate (A11). The focus of this research was on the antitumor activity of the derivatives. The efficacy of the derivative A11 (IC<sub>50</sub> < 0.3 µM) was more than 100 times higher than that of 20(R)- panaxotriol (IC<sub>50</sub> > 30 µM). In addition, A11 inhibited the protein expression and nuclear accumulation of the hypoxia-inducible factor HIF-1α in HeLa cells under hypoxic conditions in a dose-dependent manner. Moreover, A11 dose-dependently inhibited the proliferation, migration, and invasion of HeLa cells, while promoting their apoptosis. Notably, the inhibition by A11 was more significant than that by 20(R)-panaxotriol (p < 0.01) in vivo. Conclusion: To our knowledge, this is the first study to report the production of derivative A11 from 20(R)-panaxotriol and its superior antitumor activity compared to its precursor. Moreover, derivative A11 can be used to further study and develop novel antitumor drugs.