Existence and Uniqueness Theorems of Solutions for a Class Fourth-Order Boundary Value Problems

Pei, Ming He ...et al KYUNGPOOK UNIVERSITY 2001 Kyungpook mathematical journal Vol.41 No.2

In this paper, by using the Leray-Schauder degree theory, we establish the existence and uniqueness theorems of two-point boundary value problems for a class of fourth-order nonlinear differential equations. The results generalize and improve the related results in [7].

Gold Immunolocalization of Rubisco and Rubisco Activase in Pyrenoid of Chlamydomonas reinhardtii

He, Pei-Min,Zhang, Da-Bing,Chen, Geng-Yun,Liu, Qi-Gen,Wu, Wei-Ning The Korean Society of Phycology 2003 ALGAE Vol.18 No.2

The pyrenoid ultrastructure and distribution of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) and Rubisco activase in the chloroplasts of Chlamydomonas reinhardtii was studied using the immunogold localization technology with electron microscopy. There were several tubular thylakoids invading in the pyrenoid matrix to form several spokewise channels. The connections between pyrenoid matrix and stroma of chloroplast were the partial of channels. The starch sheath surrounding the pyrenoid was separated into several parts by the connections in transection. Some thylakoids were packed together near the connections in one side of the pyrenoid. Those special structures might be used to transport substance between pyrenoid and stroma of chloroplasts. With the antibody raised against the large subunits of Rubsico from C. protothecoides, the result of the gold immunolocalization of Rubisco in Chlamydomonas reinhardtii showed most of the gold particles heavily labeled the pyrenoid matrix, as well as the starch sheath matrix, and very few in the stroma of chloroplasts. The gold particle density was 880.00 $\pm$ 164.32, 190.00 $\pm$ 152.39 and 9.60 $\pm$ 5.37 ${\mu}m^{-2}$ in pyrenoid matrix, starch sheath and stroma region of chloroplast respectively (background: 5.67 $\pm$ 1.53 ${\mu}m^{-2}$). 99.59% of the total Rubiscos was calculated to be concentrated in the pyrenoid matrix and starch sheath by spatial densities. The gold immunolocalization of Rubisco activase also showed that Rubisco activase was mainly concentrated in the periphery of the pyrenoid and the starch sheath (the density was as high as 229.69 $\pm$ 96.96 ${\mu}m^{-2}$). There were very few gold particles located in the stroma of chloroplasts. These results indicated that pyrenoid surface and starch sheath was the site for Rubisco activation and $CO_2$ fixation, which supported the suggestion that pyrenoids perform photosynthesis function.

Computer simulation of stereochemical structure of biodegradable poly(d,l-lactide-co-l-lactide)

Pei-Hua He,Yan Wu,Ruo-Feng Wu 한국물리학회 2007 Current Applied Physics Vol.7 No.s1

poly(D,L-lactide-co-L-lactide) (PDLLA). The Monte Carlo model is set up according to the reactivity probabilities of two kinds of isomerRR and SS during ring-opening chain propagation. The simulation results for Tetrads and Hexads intensities are in good agreement withexperimental results for13C NMR. Some other important parameters for the stereochemical structure of PDLLA are also obtained fromRun numbers. The inuence of reactivity ratiorand feed ratiop on the stereochemical structure is analyzed and discussed in detail. Thiscomputer simulation method has proved to be a convenient method for establishing structureproperties relationships of certain poly-mers whose stereoregularity can be adjusted, such as PDLLA.

Existence and Uniqueness of Solutions of Two-point and Three-point Boundary Value Problems for nth-order Nonlinear Differential Equation

Ming He Pei ...et al KYUNGPOOK UNIVERSITY 2001 Kyungpook mathematical journal Vol.41 No.2

In this paper, we study the existence and uniqueness of solution of two-point and three-point boundary value problems for nth-order ordinary differential equation (1.1) which possess boundary conditions (1.2), (1.3), (1.4), (1.5) and (1.6) respectively, where the results of the existence and uniqueness of solutions for BVP(1.1)(1.4), BVP(1.1)(1.5) and BVP(1.1)(1.6) generalize corresponding results in [6] and [7].

Research on Injection Rate Allocation Fault Diagnosis in Integrated Separate Layer Water Injection Technology

Deli Jia,Xiaohan Pei,He Liu,Fuchao Sun,Mingxin Zhao 보안공학연구지원센터 2014 International Journal of Control and Automation Vol.7 No.12

Integrated separate layer water injection technology is an effective approach to reduce the workload of injection oilfield. The primary equipment which consists of flowmeter, motor and control value is placed underground for a long time. Injection rate allocation fault diagnosis is a problem with character of multiformity. The fault symptoms and reasons are random, and it is hard to differentiate them. Weighted fuzzy C-means clustering algorithm combining symptoms signals and fuzzy mathematics approach is used on the technology. The algorithm is based on fuzzy C-means clustering algorithm, and increase weight to each fault sample to reduce the impact of samples on fault classifications. Simulation and analysis illustrates that the algorithm is able to reflect the real structure of the sample data. And it improves the accuracy of fault classification.

Wnt/β-Catenin Promotes the Osteoblastic Potential of BMP9 Through Down-Regulating Cyp26b1 in Mesenchymal Stem Cells

Yao Xin-Tong,Li Pei-pei,Liu Jiang,Yang Yuan-Yuan,Luo Zhen-Ling,Jiang Hai-Tao,He Wen-Ge,Luo Hong-Hong,Deng Yi-Xuan,He Bai-Cheng 한국조직공학과 재생의학회 2023 조직공학과 재생의학 Vol.20 No.5

BACKGROUND: All-trans retinoic acid (ATRA) promotes the osteogenic differentiation induced by bone morphogenetic protein 9 (BMP9), but the intrinsic relationship between BMP9 and ATRA keeps unknown. Herein, we investigated the effect of Cyp26b1, a critical enzyme of ATRA degradation, on the BMP9-induced osteogenic differentiation in mesenchymal stem cells (MSCs), and unveiled possible mechanism through which BMP9 regulates the expression of Cyp26b1. METHODS: ATRA content was detected with ELISA and HPLC–MS/MS. PCR, Western blot, and histochemical staining were used to assay the osteogenic markers. Fetal limbs culture, cranial defect repair model, and micro–computed tomographic were used to evaluate the quality of bone formation. IP and ChIP assay were used to explore possible mechanism. RESULTS: We found that the protein level of Cyp26b1 was increased with age, whereas the ATRA content decreased. The osteogenic markers induced by BMP9 were increased by inhibiting or silencing Cyp26b1 but reduced by exogenous Cyp26b1. The BMP9-induced bone formation was enhanced by inhibiting Cyp26b1. The cranial defect repair was promoted by BMP9, which was strengthened by silencing Cyp26b1 and reduced by exogenous Cyp26b1. Mechanically, Cyp26b1 was reduced by BMP9, which was enhanced by activating Wnt/b-catenin, and reduced by inhibiting this pathway. b-catenin interacts with Smad1/5/9, and both were recruited at the promoter of Cyp26b1. CONCLUSIONS: Our findings suggested the BMP9-induced osteoblastic differentiation was mediated by activating retinoic acid signalling, viadown-regulating Cyp26b1. Meanwhile, Cyp26b1 may be a novel potential therapeutic target for the treatment of bone-related diseases or accelerating bone-tissue engineering.

A Novel DC Power Line Carrier Technology for the Technological Process of Water Distributor in Water Injection Well

Deli Jia,Xiaohan Pei,He Liu,Caihao Cui,Yong Yu,Meiqi Liu 보안공학연구지원센터 2014 International Journal of Smart Home Vol.8 No.6

In order to optimize the technological process of the water distributor and do further sturdy about the interference pattern of the injection layers in China, we needs reasonable and reliable methods to obtain the dynamic parameters of water injection layers. In this paper, a novel DC power line carrier technology based on the Orthogonal Frequency Division Multiplexing (OFDM) technology is proposed, aiming to save the difficult communication problem between the underground and the surface. Using the signals on the power line, it includes the modulation and demodulation techniques and filter encoding technology to transmit the data. Experimental results show that this new communication technology has high noise resistance and low error rate, provides reliable technology support and data to optimize the injection process and build the model of the dynamic parameters change among all the layers.

Load of Ag3PO4 Particles on Sulfonated Polyphenylene Sulfide Superfine Fibre with High Visible-light Photocatalytic Activity

Pei Wang,Chenchen He,Lingquan Hu,Shaohua Chen,Xianze Yin,Jing Xu,Luoxin Wang,Hua Wang 한국섬유공학회 2018 Fibers and polymers Vol.19 No.7

Ag3PO4 was loaded on sulfonated polyphenylene sulfide (SPPS) superfine fibre by a facile precipitation method. Both the structure and properties of the as-synthesized Ag3PO4/SPPS composites were characterized via XRD, SEM, EDS, XPS, FTIR, and UV-vis. The photocatalytic performance of Ag3PO4/SPPS composites was investigated via degradation of Methylene blue(MB) solution under visible light irradiation. The degradation results revealed that the photocatalytic activity of Ag3PO4/SPPS composites was greatly enhanced by the incorporation of Ag3PO4 with SPPS superfine fibre. For concentrations of AgNO3 and Na2HPO4 solutions of 0.3 M and 0.06 M in the preparation process, the Ag3PO4/SPPS composite showed higher photocatalytic activity under visible light irradiation.

<i>Yersinia pseudotuberculosis</i> Exploits CD209 Receptors for Promoting Host Dissemination and Infection

He, Ying-Xia,Ye, Cheng-Lin,Zhang, Pei,Li, Qiao,Park, Chae Gyu,Yang, Kun,Jiang, Ling-Yu,Lv, Yin,Ying, Xiao-Ling,Ding, Hong-Hui,Huang, Hong-Ping,Mambwe Tembo, John,Li, An-Yi,Cheng, Bing,Zhang, Shu-Sheng American Society for Microbiology 2019 Infection and immunity Vol.87 No.1

<P><I>Yersinia pseudotuberculosis</I> is a Gram-negative enteropathogen and causes gastrointestinal infections. It disseminates from gut to mesenteric lymph nodes (MLNs), spleen, and liver of infected humans and animals.</P><P><I>Yersinia pseudotuberculosis</I> is a Gram-negative enteropathogen and causes gastrointestinal infections. It disseminates from gut to mesenteric lymph nodes (MLNs), spleen, and liver of infected humans and animals. Although the molecular mechanisms for dissemination and infection are unclear, many Gram-negative enteropathogens presumably invade the small intestine via Peyer’s patches to initiate dissemination. In this study, we demonstrate that <I>Y. pseudotuberculosis</I> utilizes its lipopolysaccharide (LPS) core to interact with CD209 receptors, leading to invasion of human dendritic cells (DCs) and murine macrophages. These <I>Y. pseudotuberculosis</I>-CD209 interactions result in bacterial dissemination to MLNs, spleens, and livers of both wild-type and Peyer’s patch-deficient mice. The blocking of the <I>Y. pseudotuberculosis</I>-CD209 interactions by expression of O-antigen and with oligosaccharides reduces infectivity. Based on the well-documented studies in which HIV-CD209 interaction leads to viral dissemination, we therefore propose an infection route for <I>Y. pseudotuberculosis</I> where this pathogen, after penetrating the intestinal mucosal membrane, hijacks the <I>Y. pseudotuberculosis</I>-CD209 interaction antigen-presenting cells to reach their target destinations, MLNs, spleens, and livers.</P>

Monitoring the photoinduced surface catalytic coupling reaction and environmental exhaust fumes with an Ag/PDA/CuO modified 3D glass microfiber platform

Pei-Ying Lin,Gongchun He,Jyunde Chen,Atul Kumar Dwivedi,Shuchen Hsieh 한국공업화학회 2020 Journal of Industrial and Engineering Chemistry Vol.82 No.-

We demonstrate an efficient plasmon catalytic activity of a newly constructed metal–molecular–semiconductor, SERS substrate Ag/PDA/CuO@GMF. The support of tubular GMF surface and strongadhesion of PDA layer provided a uniform and well-packed distribution of Ag NPs and CuO to derive anefficient surface plasmon catalytic activity of Ag/PDA/CuO@GMF for the conversion of 4-Nitrothiophenol(4-NTP) to 4,40-Dimercaptoazobenzene (4,40-DMAB). Significant surface-enhanced Raman scattering(SERS) signal of 4,40-DMAB and efficient catalytic activities of Ag/PDA/CuO@GMF were related to thecombined cofactors, (a) a long-range electromagnetic effect of Ag NPs and (b) a stimulated chargetransfer due to the presence of CuO and Ag NPs. In addition, charge redistributions among adsorbent 4,40-DMAB and metal segments (Ag and CuO) via PDA played an important role in obtaining enhanced surfaceplasmon induced catalytic activities. Moreover, we utilized the structural properties of the 3D microfiberto enhance the applicability of the substrate. It was further demonstrated that the substrate could be usedto adsorb and detect the gaseous pollutants in the environment, thus providing an effective method forrapid on-site analysis.