In Vivo Evaluation of Curcumin-loaded Nanoparticles in a A549 Xenograft Mice Model

Yin, Hai-Tao,Zhang, De-Geng,Wu, Xiao-Li,Huang, Xin-En,Chen, Gang Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.1

Curcumin (Cum) has been reported to have potential chemo-preventive and chemotherapeutic activity through influencing various processes, inducing cell cycle arrest, differentiation and apoptosis in a series of cancers. However, the poor solubility of Cum limits its further applications in the treatment of cancer. We have previously reported Cum-loaded nanoparticles (Cum-NPs) prepared with amphilic methoxy poly(ethylene glycol)-polycaprolactone (mPEG-PCL) block copolymers. The current study demonstrated superior antitumor efficacy of Cum-NPs over free Cum in the treatment of lung cancer. In vivo evaluation further demonstrated superior anticancer effects of Cum-NPs by delaying tumor growth compared to free Cum in an established A549 transplanted mice model. Moreover, Cum-NPs showed little toxicity to normal tissues including bone marrow, liver and kidney at a therapeutic dose. These results suggest that Cum-NPs are effective to inhibit the growth of human lung cancer with little toxicity to normal tissues, and could provide a clinically useful therapeutic regimen. They thus merit more research to evaluate the feasibility of clinical application.

Adsorption of Pb(II) from aqueous solution to Ni-doped bamboo charcoal

Yin Wang,Xuejiang Wang,Xin Wang,Mian Liu,Zhen Wu,Lianzhen Yang,Siqing Xia,Jianfu Zhao 한국공업화학회 2013 Journal of Industrial and Engineering Chemistry Vol.19 No.1

Bamboo charcoal (BC) obtained by pyrolysis of Makino bamboo in the absence of oxygen was used as support for the preparation of Ni-doped adsorbent (Ni-BC). The low-cost composite was characterized and used as an adsorbent for Pb(II) removal from water. The results showed that both BET surface area and total pore volume of Ni-BC increased. The adsorption of Pb(II) strongly depended on solution pH,temperature and ionic strength. The adsorption isotherms followed Langmuir isotherm model well, and the maximum adsorption capacities of Pb(II) at 298 K were 25.0 and 142.7 mg/g for BC and Ni-BC,respectively. The adsorption processes were well fitted by pseudo-second-order kinetic model. Thermodynamic parameters showed that the adsorptions of Pb(II) onto both adsorbents were feasible,spontaneous, and exothermic under the studied conditions. The spent Ni-BC could be readily regenerated for reuse.

Carbon Nanotubes-Supported Well-Dispersed Pd Nanoparticles for the Efficiently Selective Hydrogenation of Benzoic Acid to Synthesize Cyclohexane Carboxylic Acid

Yin Hu,Wei Chen,Qi Wu,Xin Xie,Weiguo Song 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2019 NANO Vol.14 No.1

Pd and carbon nanotube (CNT) composites were prepared by well-dispersed deposition of Pd nanoparticles on commercial CNT, and applied to the selective hydrogenation of benzoic acid (BA) to synthesize cyclohexane carboxylic acid (CCA). The catalysts and the hydrogenation products were analyzed by XRD, TEM, TG, FTIR, UV-Vis absorption, GC and GC-MS, respectively. Hydrogenation process was also optimized through varying the reaction parameters. The results demonstrate that Pd/CNT catalysts possess the highest hydrogenation efficiency, give the full conversion of BA and 100.0% selectivity towards CCA at the optimal hydrogenation conditions, by comparing with some commercial hydrogenation catalysts and Pd/C catalysts with commercial carbonaceous supports. The excellent hydrogenation performance of Pd/CNT is attributed to the stable crystalline CNT support and the high dispersion of Pd nanoparticles. In addition, the protic solvent is also beneficial to lower the activation energy barrier of BA hydrogenation, and further to improve the hydrogenation rate. This work implies that CNT can be potentially chosen as an effective carbonaceous support to prepare Pd/C catalyst with an outstanding performance of BA selective hydrogenation.

Human Glutathione S-Transferase P1 Suppresses MEKK1-mediated Apoptosis by Regulating MEKK1 Kinase Activity in HEK293 Cells

Zhimin Yin,Xin Zhao,Yumei Fan,Jiayin Shen,Yifan Wu 한국분자세포생물학회 2006 Molecules and cells Vol.21 No.3

Experimental and Modeling Analysis of Hydrogen Motion Behavior in Welding Zone of 2.25Cr–1Mo–0.25 V Steel Welded Joint with Hydrogen Corrosion

Changdong Yin,Dongdong Ye,Zhou Xu,Jianjun Chen,Yiwen Wu,Houli Liu,Xin Ge,Qiang Liu,Guiling Zeng 대한금속·재료학회 2023 METALS AND MATERIALS International Vol.29 No.8

Understanding and solving the problem of hydrogen damage due to the degradation of service performance of high-strengthsteel caused by hydrogen penetration is a milestone for the reliability improvement of high-end hydrogen energy equipment. From the perspective of microscopic hydrogen movement behavior, this study investigated the permeability of hydrogenin base metal (BM) and welded metal (WM) of 2.25Cr–1Mo–0.25 V steel welded joint through electrochemical hydrogenpermeation test, and the analytical models of hydrogen penetration and hydrogen diffusion in the welding zone were established,and the effect of adding hydrogen-resistant coatings (HRCs) on hydrogen movement behavior in steel substrate wasstudied. The results showed that the diffusivity of hydrogen in WM of the welded joint was significantly lower than thatin BM, and the hydrogen concentration in BM was higher than that in WM at the beginning of hydrogenation without theaddition of HRCs protection, while it tended to be consistent at the later stage. However, the hydrogen concentration in WMwas lower than that in BM after adding HRCs and would be always higher than that in BM after a certain period of time,and the difference was directly related to the hydrogen resistance of the coatings. Additionally, the difference between theintermediate and outer layers for the welding zone steel substrate with and without HRCs presented a trend of rapid increasefollowed by a slow decrease. These findings could provide an important reference value for hydrogen damage protectionmeasures and related researches on HRCs.

Echinacoside Prevents Sepsis-Induced Myocardial Damage via Targeting SOD2

Jingxiang Wu,Xingji You,Xin Li,Zuojing Zhang,Xiaoxuan Zhang,Yibo Yin,Xinru Yuan 한국식품영양과학회 2024 Journal of medicinal food Vol.27 No.2

Echinacoside (ECH) is a prominent naturally occurring bioactive compound with effects of alleviatingmyocardial damage. We aimed to explore the beneficial effects of ECH against sepsis-induced myocardial damage andelucidate the potential mechanism. Echocardiography and Masson staining demonstrated that ECH alleviates cardiac functionand fibrosis in the cecal ligation and puncture (CLP) model. Transcriptome profiling and network pharmacology analysisshowed that there are 51 overlapping targets between sepsis-induced myocardial damage and ECH. Subsequently, chemicalcarcinogenesis-reactive oxygen species (ROS) were enriched in multiple targets. Wherein, SOD2 may be the potential targetof ECH on sepsis-induced myocardial damage. Polymerase chain reaction results showed that ECH administration couldmarkedly increase the expression of SOD2 and reduce the release of ROS. Combined with injecting the inhibitor of SOD2, thebeneficial effect of ECH on mortality, cardiac function, and fibrosis was eliminated, and release of ROS was increased afterinhibiting SOD2. ECH significantly alleviated myocardial damage in septic mice, and the therapeutic mechanism of ECH isachieved by upregulating SOD2 which decreased the release of ROS.

Realgar transforming solution-induced differentiation of NB4 cell by the degradation of PML/RARa partially through the ubiquitin–proteasome pathway

Yang Hai,Xin Wang,Peng Song,Jian-yin Li,Longhe Zhao,Fei Xie,Xiang-min Tan,Qin-Jian Xie,Lan Yu,Yang Li,Zhengrong Wu,Hong Yu Li 대한약학회 2019 Archives of Pharmacal Research Vol.42 No.8

PML/retinoic acid receptor alpha (RARa), as ahallmark of acute promyeloid leukemia (APL), is directlyrelated to the outcome of clinical APL remedy. It isreported that arsenicals can effectively degrade PML/RARa, such as arsenic trioxide and realgar. However, thehigh toxicity or insolubility have hampered their clinicalapplications. Realgar transforming solution (RTS) wasproduced from realgar by bioleaching process in our lab. Previous studies demonstrated that RTS had a significantanti-cancer ability on chronic myeloid leukemia throughoncoprotein degradation. The capacity of RTS on treatingAPL is what is focused on in this study. The results showedthat RTS had a noticeable sensitivity in NB4 cell, and RTSremarkably down-regulated PML/RARa expression andinduced cell differentiation. Further, RTS could accumulatePML/RARa into the nuclear bodies and then executedegradation, which could be reversed by proteasomeinhibitor MG132. The results also exhibited that thereduction of RTS-induced PML/RARa expression accompaniedby the elevation of ubiquitin and SUMO-1 proteinexpression. Finally, PML and SUMO-1 had been demonstratedto be co-localized after RTS treatment byimmunofluorescence co-localization assay and immunoprecipitationassay. In conclusion, these results suggestedthat RTS-induced cell differentiation may attribute to thePML/RARa degradation partially through the ubiquitin–proteasome pathway.

Overview of systematic reviews with meta-analyses on acupuncture in post-stroke cognitive impairment and depression management

Caroline Yik-fong Hung,Xin-yin Wu,Vincent Chi-ho Chung,Endy Chun-hung Tang,Justin Che-yuen Wu,Alexander Yuk-lun Lau 한국한의학연구원 2019 Integrative Medicine Research Vol.8 No.3

Background: Acupuncture has been using as an alternative non-pharmacological therapy in the management of post stroke depression and cognitive impairment but its effectiveness and safety remain controversial. We conducted an overview of systematic reviews with meta-analyses to evaluate the evidence on the effect of acupuncture in the treatment of stroke with conventional medicine intervention. Methods: Systematic reviews summarized the treatment effects of acupuncture for post stroke cognitive impairment and post stroke depression were considered eligible. Methodological quality of included systematic reviews was assessed using A MeaSurement Tool to Assess systematic Reviews 2 (AMSTAR 2). Results: Four systematic reviews on post stroke cognitive impairment and ten systematic reviews on post stroke depression with good methodological quality were included. Meta-analyses revealed that acupuncture plus cognitive rehabilitation; and acupuncture or moxibustion plus cognitive rehabilitation, versus cognitive rehabilitation demonstrated statistically significant increase in Mini-Mental State Examination scores in compared to cognitive rehabilitation after 4 weeks treatment [Pooled weighted mean difference (WMD) = 3.14, 95% confidence interval (CI) = 2.06 to 4.21, I2 = 36%]; and (Pooled WMD = 3.22, 95% CI = 2.09 to 4.34, I2 = 0%). Furthermore, acupuncture versus antidepressant demonstrated statistically significant improve depression measured by increasing in 17-item Hamilton Depression Rating Scale in comparing to cognitive rehabilitation after 2 weeks treatment (Pooled WMD= -2.34, 95% CI= -3.46 to -1.22, I2 = 5%). Acupuncture usage was not associated with increased risk of adverse events. Conclusions: Acupuncture is safe and improves cognitive function and depressive disorder without obvious serious adverse events for post stroke patients.

The conceptual design of grid-connected wind turbine based on speed regulating differential mechanism

Xiaoming Rui,Rui Su,Xin Wu,Qidong Yin 대한기계학회 2014 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.28 No.6

In view of problems arising from the grid-connected wind turbine with the frequency converter, we propose a conceptual design ofgrid-connected wind turbine based on speed regulating differential mechanism (SRDM). The SRDM regulates its output speed andtorque self-adaptively referring to wind rotor and makes the synchronous generator (SG) keep a constant speed to output electricity insteady frequency without the normal frequency converter and it has excellent low voltage ride through (LVRT). This paper offers thekinematics and dynamics analysis of the designed wind turbine and builds the SIMULINK model based on FAST (fatigue, aerodynamics,structures and turbulence) software. The simulation of speed, torque and power of wind rotor under average wind speed of 12 m/s andturbulence intensity of 20% can verify the feasibility of the designed wind turbine.

Serum Zinc Status and Helicobacter Pylori Infection in Gastric Disease Patients

Zhang, Wen-Hua,Wu, Xiao-Jing,Niu, Jing-Xiu,Yan, Hao,Wang, Xin-Zhuo,Yin, Xiao-Dong,Pang, Yan Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.10

The role of Helicobacter pylori status and serum zinc value in gastric disease patients and healthy controls were investigated. Cases used in this work were 45 gastric cancer patients, 44 with peptic ulcers, 52 suffering gastritis and 64 healthy controls, all diagnosed histologically with the controls undergoing medical checkups. Helicobacter pylori status and serum levels of Zn were determined by 13C-urea breath test and flame atomic absorption spectrophotometer, respectively. Our study showed that Helicobacter pylori infection has no change in gastritis, peptic ulcer and gastric cancer group, on the contrast, serum levels of Zn were significantly reduced in gastritis, peptic ulcer and gastric cancer group, compared with healthy controls, and the higher the Zn levels are, the more increased risk of gastric cancer. Helicobacter pylori infection is a cause of gastritis, peptic ulcers and even gastric cancer, while serum zinc level is an indicator of protection of gastric membranes against damage.