Research on the Temperature & Humidity Monitoring System in the Key Areas of the Hospital Based on the Internet of Things

Benzhen Guo,Xiaojing Wang,Xiao Zhang,Jingjing Yang,Zhihui Wang 보안공학연구지원센터 2016 International Journal of Smart Home Vol.10 No.7

This paper designs an internet of things-based temperature & humidity monitoring system in the key areas of the hospital to address the problem that the temperature and humidity monitoring systems are independent from each other in the hospital. The establishment of a hospital-level temperature and humidity monitoring platform is able to realize the integrated monitoring and management on such areas as ICU, pharmacy and operation room etc, where both of the temperature and humidity must be monitored. In every temperature and humidity acquisition node, the collected environmental data about the indoor humidity and temperature will be sent over the 433M wireless sensor network to the internet of things (IoT) gateway, through which data will be uploaded via wifi or the wired access network within the hospital to the background server, where in addition to data collection and analysis, various reports will be generated and control commands will be transferred. Then all of the medical staffs can make a real-time inquiry and control on the environmental data by connecting their intelligent terminals (such as cell phone) to the server. Actually when the humidistat thermostat controller is designed according to the DDC principle and the integral-separation PID control algorithm, the control on the central air-conditioning units is able to realize the real-time control of constant temperature and humidity. Also a prototype system has been established to conduct the functional verification. The experiment shows that this system is able to run steadily with precise data collection and reliable control.

Interface damage mechanism of rubber cord composite material

Xiaohui Guo,Xiaojing Yuan,Guangyong Liu,Hefang Qiu,Fanfan Cui 한양대학교 청정에너지연구소 2023 Journal of Ceramic Processing Research Vol.24 No.1

In order to study the interface failure mechanism of rubber cord composites laid at symmetrical angles, a representativevolume element was established by the finite element method to simulate the stress distribution at the mesoscopic level ofrubber cord composites. The energy-based power law is used to simulate the damage evolution of the cord-rubber interface. The results show that the interface damage is caused by the torque that the cord bears during the stretching process, and theinterface assumes the role of transmitting torque in this process, so the failure occurred.The initial interface stiffness is a vitalbonding interface parameter that affects the interface damage evolution, and the initial stiffness is positively correlated withthe interface damage evolution variable. The increase in the included angle of the cord will cause the torque on the cord toincrease, resulting in aggravated interface failure.

Review : Novel Properties for Endoglucanase Acquired by Cell-Surface Display Technique

( Baosheng Shi ),( Xiaojing Ke ),( Hongwei Yu ),( Jing Xie ),( Yingmin Jia ),( Runfang Guo ) 한국미생물 · 생명공학회 2015 Journal of microbiology and biotechnology Vol.25 No.11

In order to improve the stability of endoglucanase under thermal and acidic conditions, the endoglucanase gene was fused to the N-terminus of the Saccharomyces cerevisiae pir gene, encoding the cell wall protein PIR. The fusion gene was transformed into Pichia pastoris GS115 for expression. A resulting strain with high expression and high activity was identified by examining resistance to Geneticin 418, Congo red staining, and quantitative analysis of enzyme activity. SDS-PAGE analysis revealed that the endoglucanase was successfully displayed on the yeast cell surface. The displayed endoglucanase (DEG) showed maximum activity towards sodium carboxyl methyl cellulose at approximately 275 IU/g cell dry weight. DEG exhibited greater than 60% residual activity in the pH range 2.5-8.5, higher than free endoglucanase (FEG), which had 40% residual activity at the same pH range. The highest tolerated temperature for DEG was 70ºC, much higher than that of FEG, which was approximately 50ºC. Moreover, DEG showed 91.1% activity at 65ºC for 120 min, while FEG only kept 77.8% residual activity over the same period. The half-life of DEG was 270 min at 65ºC, compared with only 150 min for FEG. DEG could be used repeatedly at least three times. These results suggest that the DEG has broad applications as a yeast whole-cell biocatalyst, due to its novel properties of high catalytic efficiency, acid-thermal stabilities, and reusability.

Arginine Supplementation Recovered the IFN-gamma-Mediated Decrease in Milk Protein and Fat Synthesis by Inhibiting the GCN2/eIF2alpha Pathway, Which Induces Autophagy in Primary Bovine Mammary Epithelial Cells

Liancheng Lei,Xiaojing Xia,Yanyi Che,Yuanyuan Gao,Shuang Zhao,Changjin Ao,Hongjian Yang,Juxiong Liu,Guo-wen Liu,Wenyu Han,Yuping Wang 한국분자세포생물학회 2016 Molecules and cells Vol.39 No.5

During the lactation cycle of the bovine mammary gland, autophagy is induced in bovine mammary epithelial cells (BMECs) as a cellular homeostasis and survival mecha-nism. Interferon gamma (IFN-) is an important antiproliferative and apoptogenic factor that has been shown to induce autophagy in multiple cell lines in vitro. However, it remains unclear whether IFN- can induce autophagy and whether autophagy affects milk synthesis in BMECs. To understand whether IFN- affects milk synthesis, we isolated and purified primary BMECs and investigated the effect of IFN- on milk synthesis in primary BMECs in vitro. The results showed that IFN- significantly inhibits milk synthesis and that autophagy was clearly induced in primary BMECs in vitro within 24 h. Interestingly, autophagy was observed following IFN- treatment, and the inhibition of autophagy can improve milk protein and milk fat syn-thesis. Conversely, upregulation of autophagy decreased milk synthesis. Furthermore, mechanistic analysis con-firmed that IFN- mediated autophagy by depleting argi-nine and inhibiting the general control nonderepressible-2 kinase (GCN2)/eukaryotic initiation factor 2 (eIF2) signaling pathway in BMECs. Then, it was found that arginine supplementation could attenuate IFN--induced autophagy and recover milk synthesis to some extent. These findings may not only provide a novel measure for preventing the IFN--induced decrease in milk quality but also a useful therapeutic approach for IFN--associated breast diseases in other animals and humans.

Proteasome subunit beta type 1 interacts directly with Rheb and regulates the cell cycle in Cashmere goat fetal fibroblasts

Yanfeng Wang,Xudong Guo,Zhigang Wang,Zhixin Guo,Mingtao Liu,Keyu Zhao,Xue Feng,Xu Zheng,Xiaojing Wang,Huifang Hao 한국통합생물학회 2017 Animal cells and systems Vol.21 No.5

PSMB1 (proteasome subunit beta type 1) is a core component of the 20S proteasome, and based on its structure, it might have a crucial function in the transcription of certain genes. Rheb (Ras homolog enriched in brain) is a Ras-like small GTPase that acts as an upstream positive regulator of the mechanistic target of rapamycin complex 1 (mTORC1) pathway. We cloned and characterized PSMB1 (KY310590.1) to determine its function in cell cycle progression and proliferation of goat fetal fibroblasts (GFbs). Further, by yeast two-hybrid screen and coimmunoprecipitation, we conformed that PSMB1 interacts directly with Rheb. An siRNA was designed and expressed targeting PSMB1 mRNA in GFbs and inducing cell cycle arrest. Rheb overexpression in GFbs significantly increased the number of S phase cells and growth efficiency compared with control cells. These data indicate that PSMB1 and Rheb have important functions in the cell cycle and proliferation of GFbs, indicating that their interaction governs many processes in GFbs.

Phase structure, electrical properties, and stability of 0.96(K0.48Na0.52)1xLixNbO3e0.04Bi0.5Na0.5ZrO3 lead-free piezoceramics

Xiaopeng Wang,Jia-gang Wu,Ting Zheng,Xiaojing Cheng,Binyu Zhang,Dingquan Xiao,Jian-guo Zhu 한국물리학회 2014 Current Applied Physics Vol.14 No.5

In this work, 0.96(K0.48Na0.52)1xLixNbO3e0.04Bi0.5Na0.5ZrO3 lead-free piezoceramics were prepared using the conventional solid state reaction method in order to attain both a high TC and an enhanced d33. The effect of Li content on their phase structure, electrical properties, and stability was systematically investigated. These results indicate that adding Li could result in the increase of TC and the decrease of TOeT. Here we obtain the ceramics with an orthogonaletetragonal phase boundary in the composition of x ¼ 0.02, and then enhanced piezoelectric properties and a high Curie temperature (i.e., d33 w 255 pC/N, kp w 41%, and TC w 360 C) were observed, showing the realization of our objective. In addition, a good stability of piezoelectric and ferroelectric properties has been shown in such a material system.

Selection of Reference Genes for Gene Expression Studies in Porcine Whole Blood and Peripheral Blood Mononuclear Cells under Polyinosinic:Polycytidylic Acid Stimulation

Wang, Jiying,Wang, Yanping,Wang, Huaizhong,Hao, Xiaojing,Wu, Ying,Guo, Jianfeng Asian Australasian Association of Animal Productio 2014 Animal Bioscience Vol.27 No.4

Investigating gene expression of immune cells of whole blood or peripheral blood mononuclear cells (PBMC) under polyinosinic:polycytidylic acid (poly I:C) stimulation is valuable for understanding the immune response of organism to RNA viruses. Quantitative real-time PCR (qRT-PCR) is a standard method for quantification of gene expression studies. However, the reliability of qRT-PCR data critically depends on proper selection of reference genes. In the study, using two different analysis programs, geNorm and NormFinder, we systematically evaluated the gene expression stability of six candidate reference genes (GAPDH, ACTB, B2M, RPL4, TBP, and PPIA) in samples of whole blood and PBMC with or without poly I:C stimulation. Generally, the six candidate genes performed a similar trend of expression stability in the samples of whole blood and PBMC, but more stably expressed in whole blood than in PBMC. geNorm ranked B2M and PPIA as the best combination for gene expression normalization, while according to NormFinder, TBP was ranked as the most stable reference gene, followed by B2M and PPIA. Comprehensively considering the results from the two programs, we recommended using the geometric mean of the three genes, TBP, PPIA and B2M, to normalize the gene expression of whole blood and PBMC with poly I:C stimulation. Our study is the first detailed survey of the gene expression stability in whole blood and PBMC with or without poly I:C stimulation and should be helpful for investigating the molecular mechanism involved in porcine whole blood and PBMC in response to poly I:C stimulation.

Highly Durable and Robust Superhydrophobic/Superoleophilic Cotton Fabric with Well-designed Roughness for Oil/Water Separation

Jiangtao Hu,Qianhong Gao,Lu Xu,Minglei Wang,Maojiang Zhang,Kuo Zhang,Xiaojing Guo,Weihua Liu,Guozhong Wu 한국섬유공학회 2018 Fibers and polymers Vol.19 No.7

Herein we report a simple and reproducible method for fabricating highly durable and robust superhydrophobic and superoleophilic cotton fabrics via simultaneous radiation-induced graft polymerization of glycidyl methacrylate and subsequent chemical modifications with aminopropyltriethoxysilane and hexamethyldisilazane. The chemical structure and the surface topography of the pristine and the modified cotton fabrics were investigated in detail by ATR-FTIR, XPS, and 29Si NMR, and a grafting layer was successfully immobilized onto the surface of the cotton fabric by forming covalent bonds. Multi-dimensional surface roughness was created by combining micro-sized fibers of the cotton fabric, nanoscaled protuberances of the grafting chain, and molecular level spherical projection points of silicon methyl. The superhydrophobic cotton fabric exhibited long-term stability, ultra-high durability and robustness, and maintained its properties even after 25 wash cycles. The fabric also showed excellent water repellency with a water contact angle of 153 o and a high efficiency of oil/water separation (98 %). The superhydrophobic/superoleophilic cotton fabric developed in the present work exhibits important potential applications in superhydrophobic textiles and oil/water separation.

TsMIP6 enhances the tolerance of transgenic rice to salt stress and interacts with target proteins

Linlin Sun,Guohong Yu,Xiaori Han,Shichao Xin,Xiaojing Qiang,Linlin Jiang,Shuhui Zhang,Xian-guo Cheng 한국식물학회 2015 Journal of Plant Biology Vol.58 No.5

Aquaporins (AQPs), a large family of channel proteins in plants, play an important role in regulating the balance of osmotic potential in cells. We isolated an AQP gene, TsMIP6, from the halophyte Thellungiella salsuginea and functionally characterized it in transgenic rice (Oryza sativa). This gene belongs to a subfamily of tonoplast intrinsic proteins and is localized at the plasma membrane. Real-time PCR showed that expression of TsMIP6 in shoots or roots of T. salsuginea was markedly induced by salinity, whereas its ectopic expression in ‘Kitaake’ lines of rice significantly increased plant tolerance to salt stress. Physiological data suggested that TsMIP6 is involved in regulating ion homeostasis and water channel activity in salt-stressed transgenic rice. Heterologous expression analysis indicated that TsMIP6 specifically interacts with a member of the glycoside hydrolase family 64 protein #617 in yeast cells. This suggests that the relationship between TsMIP6 and #617 has a crucial role in mediating osmotic balance in plant cells. Moreover, TsMIP6 might help to modulate the transport of some neutral molecules and may function through a pathway regulating solute equilibrium to maintain osmotic potential.