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Guo, Zhixin,Wang, Yanfeng,Feng, Xue,Bao, Chaogetu,He, Qiburi,Bao, Lili,Hao, Huifang,Wang, Zhigang Asian Australasian Association of Animal Productio 2016 Animal Bioscience Vol.29 No.11
Mammalian target of rapamycin complex 1 (mTORC1) is a central regulator of cell growth and metabolism and is sufficient to induce specific metabolic processes, including de novo lipid biosynthesis. Elongation of very-long-chain fatty acids 1 (ELOVL1) is a ubiquitously expressed gene and the product of which was thought to be associated with elongation of carbon (C) chain in fatty acids. In the present study, we examined the effects of rapamycin, a specific inhibitor of mTORC1, on ELOVL1 expression and docosahexaenoic acid (DHA, C22:6 n-3) synthesis in bovine mammary epithelial cells (BMECs). We found that rapamycin decreased the relative abundance of ELOVL1 mRNA, ELOVL1 expression and the level of DHA in a time-dependent manner. These data indicate that ELOVL1 expression and DHA synthesis are regulated by mTORC1 in BMECs.
Xiaodan Ma,Zhixin Guo,Wenhua Zhao,Li Chen 대한약리학회 2023 The Korean Journal of Physiology & Pharmacology Vol.27 No.6
Sweroside is a natural monoterpene derived from Swertia pseudochinensis Hara. Recently, studies have shown that sweroside exhibits a variety of biological activities, such as anti-inflammatory, antioxidant, and hypoglycemic effects. However, its role and mechanisms in high glucose (HG)-induced renal injury remain unclear. Herein, we established a renal injury model in vitro by inducing human renal tubular epithelial cell (HK-2 cells) injury by HG. Then, the effects of sweroside on HK-2 cell activity, inflammation, reactive oxygen species (ROS) production, and epithelial mesenchymal transition (EMT) were observed. As a result, sweroside treatment ameliorated the viability, inhibited the secretion of inflammatory cytokines (TNF-α, IL-1β, and VCAM-1), reduced the generation of ROS, and inhibited EMT in HK-2 cells. Moreover, the protein expression of SIRT1 was increased and the acetylation of p65 NFkB was decreased in HK-2 cells with sweroside treatment. More importantly, EX527, an inhibitor of SIRT1, that inactivated SIRT1, abolished the improvement effects of sweroside on HK-2 cells. Our findings suggested that sweroside may mitigate HGcaused injury in HK-2 cells by promoting SIRT1-mediated deacetylation of p65 NF-kB.
Yanfeng Wang,Xudong Guo,Zhigang Wang,Zhixin Guo,Mingtao Liu,Keyu Zhao,Xue Feng,Xu Zheng,Xiaojing Wang,Huifang Hao 한국통합생물학회 2017 Animal cells and systems Vol.21 No.5
PSMB1 (proteasome subunit beta type 1) is a core component of the 20S proteasome, and based on its structure, it might have a crucial function in the transcription of certain genes. Rheb (Ras homolog enriched in brain) is a Ras-like small GTPase that acts as an upstream positive regulator of the mechanistic target of rapamycin complex 1 (mTORC1) pathway. We cloned and characterized PSMB1 (KY310590.1) to determine its function in cell cycle progression and proliferation of goat fetal fibroblasts (GFbs). Further, by yeast two-hybrid screen and coimmunoprecipitation, we conformed that PSMB1 interacts directly with Rheb. An siRNA was designed and expressed targeting PSMB1 mRNA in GFbs and inducing cell cycle arrest. Rheb overexpression in GFbs significantly increased the number of S phase cells and growth efficiency compared with control cells. These data indicate that PSMB1 and Rheb have important functions in the cell cycle and proliferation of GFbs, indicating that their interaction governs many processes in GFbs.
RETURN CONTROL OF ELECTRONIC POWER STEERING UNEQUIPPED WITH AN ANGLE SEN
Shaosong Li,Luping Guo,Gaojian Cui,Zhixin Yu,Xiaohui Lu,Guodong Wang 한국자동차공학회 2019 International journal of automotive technology Vol.20 No.2
A steering wheel may not be able to return to the center position due to friction torque in its mechanism. Thus, in this study, a return controller is developed to improve the returnability of an electric power steering vehicle without an angle sensor. The aligning moment is well estimated on the basis of a Kalman filter estimator. A trigger rule is also proposed to switch between assist and return controls. Finally, the compensation current for the return control is regulated in accordance with the aligning moment and vehicle speeds. Simulation and real vehicle results show that the proposed control system can significantly improve the returnability of the steering system.
Recombinant Goat VEGF164 Increases Hair Growth by Painting Process on the Skin of Shaved Mouse
Bao, Wenlei,Yin, Jianxin,Liang, Yan,Guo, Zhixin,Wang, Yanfeng,Liu, Dongjun,Wang, Xiao,Wang, Zhigang Asian Australasian Association of Animal Productio 2014 Animal Bioscience Vol.27 No.9
To detect goat vascular endothelial growth factor (VEGF)-mediated regrowth of hair, full-length VEGF164 cDNA was cloned from Inner Mongolia cashmere goat (Capra hircus) into the pET-his prokaryotic expression vector, and the recombinant plasmid was transferred into E. coli BL21 cells. The expression of recombinant $6{\times}his-gVEGF164$ protein was induced by 0.5 mM isopropyl thio-${\beta}$-D-galactoside at $32^{\circ}C$. Recombinant goat VEGF164 (rgVEGF164) was purified and identified by western blot using monoclonal anti-his and anti-VEGF antibodies. The rgVEGF164 was smeared onto the dorsal area of a shaved mouse, and we noted that hair regrowth in this area was faster than in the control group. Thus, rgVEGF164 increases hair growth in mice.
Mahfuz, Shad,Song, Hui,Liu, Zhongjun,Liu, Xinyu,Diao, Zipeng,Ren, Guihong,Guo, Zhixin,Cui, Yan Asian Australasian Association of Animal Productio 2018 Animal Bioscience Vol.31 No.5
Objective: This experiment was conducted to evaluate the effects of golden needle mushroom (Flammulina velutipes) stem waste (FVW), on organic eggs production, calcium utilization, antibody response, serum immunoglobulin, and serum cytokine concentration at early phase of production in laying hens. Methods: A total 210, 19 weeks old aged ISA Brown layers were randomly assigned into 5 equal treatment groups, with 7 replications of 6 hens each. Dietary treatment included a standard basal diet as control; antibiotic (0.05% flavomycin); 2% FVW; 4% FVW; and 6% FVW. The experimental duration was 10 weeks. Results: There was no significant differences (p>0.05) on hen day egg production, egg weight, egg mass, feed intake, and feed conversion ratio (FCR) among experimental groups. Unmarketable eggs were significantly lower (p<0.05) both in 4% FVW and 6% FVW fed groups than control group. The calcium retention and calcium in egg shell deposition were significantly higher (p<0.05) in FVW inclusion groups than control and antibiotic groups. Antibody titers against Newcastle diseases were significantly higher (p<0.05) in 6% FVW fed group (except combined with 4% FVW at day 147) and infectious bronchitis were significantly higher (p<0.05) in FVW fed groups (except 2% FVW and 4% FVW at day 161) than control and antibiotic groups. Serum immunoglobulin sIgA was significantly higher (p<0.05) in all levels of FVW and IgG was significantly higher (p<0.05) in 4% FVW than control and antibiotic groups. Serum cytokine concentration interleukin-2 (IL-2) was significantly higher (p<0.05) in 6% FVW; IL-6 and tumor necrotic $factor-{\alpha}$ were significantly higher (p<0.05) both in 4% FVW and 6% FVW than control and antibiotic groups; IL-4 was significantly higher (p<0.05) in antibiotic, 2% FVW and 4% FVW fed groups than control. Conclusion: F. velutipes mushroom waste can be used as a novel substitute for antibiotic for organic egg production and sound health status in laying hens.
Li, Yao,Zhu, Shenmin,Liu, Qinglei,Gu, Jiajun,Guo, Zaiping,Chen, Zhixin,Feng, Chuanliang,Zhang, Di,Moon, Won-Jin The Royal Society of Chemistry 2012 Journal of materials chemistry Vol.22 No.6
<P>A high-performance anode material was prepared from a hierarchically structured activated carbon which contains <I>in situ</I> graphene and nano-graphite. The activated carbon was immersed in a solution of SnCl<SUB>2</SUB>·2H<SUB>2</SUB>O and subjected to ultrasound. As a result, nanoparticles of SnO<SUB>2</SUB> were uniformly deposited on the surface of the activated carbon. The composite material was then coated with a thin layer of carbon by soaking it in a sucrose solution, followed by carbonization of the adsorbed sucrose at 500 °C. The resulting composite showed an outstanding high-rate cycling performance that can deliver an initial discharge capacity of 1417 mAh g<SUP>−1</SUP> and maintain a discharge capacity of more than 400 mAh g<SUP>−1</SUP> after 100 cycles at a high current density of 1000 mA g<SUP>−1</SUP>. This outstanding electrochemical performance is likely to be related to a unique combination of the excellent electrical conductivity of the activated carbon with graphite layers formed inside, its hierarchical pore structure which enhances lithium-ion transportation, and the carbon coating which alleviates the effects of volume changes, shortens the distance for Li<SUP>+</SUP> diffusion, facilitates the transmission of electrons, and keeps the structure stable.</P> <P>Graphic Abstract</P><P>Carbon-coated SnO<SUB>2</SUB>@C nanocomposite with hierarchically porous structures and graphite layers inside was prepared by ultrasound and hydrothermal treatment, which showed an outstanding high-rate cycling performance for lithium-ion battery. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c1jm14290a'> </P>