Production of 3-hydroxypropionic acid by recombinant Klebsiella pneumoniae based on aeration and ORP controlled strategy

Jian-Guo Zhu,Xiao-Jun Ji,Jun Du,Shuang Li,Yue-Yue Ding,He Huang 한국화학공학회 2009 Korean Journal of Chemical Engineering Vol.26 No.6

A biosynthetic pathway for the production of 3-hydroxypropionic acid (3-HP) from glycerol was established in recombinant Klebsiella pneumoniae by introducing the aldehyde dehydrogenase gene from Escherichia coli. The activity of aldehyde dehydrogenase, which oxidized 3-hydroxypropionaldehyde (3-HPA) to 3-HP, was detected and 3-HP was produced by the recombinant strains. Three different oxygen supply strategies, associated with measuring the oxidoreduction potential (ORP) during the fermentation under these conditions, were adopted for higher production of 3-HP by the recombinant cells. About 0.8 g/l 3-HP and more 1,3-propanediol production by the recombinant Klebsiella pneumoniae were obtained under completely aerobic conditions. Under micro-aerobic conditions, 3-HP production could be increased to 2.2 g/l and 1,3-propanediol production was almost the same as in the original strain. Under the anaerobic conditions, 1,3-propanediol was the main product and about 1.3 g/l 3-HP was produced. Finally, 3-HP production of the recombinant strain was increased to 2.8 g/l under micro-aerobic condition with a further two-stage ORP controlled strategy.

Local Field Enhancement Due to Multiple Scattering in Random Sb-SiN Thin Films

Ruo-Jian Zhu,Ding-Rong Ou,Guo-Fan Jin,Jia Wang,Jing Zhu 한국물리학회 2005 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.47 No.1

Random Sb-SiN films consisting of random small Sb particles embedded in a SiN thin film were deposited by radio-frequency magnetron sputtering. Specimens comprising in order a random Sb- SiN film and an optical-phase-change recording layer were exposed to a focused laser beam. It was shown that, with a random Sb-SiN layer deposited above the recording layer, the ablation of the recording layer occurred much faster and under much lower power than that of a single recording layer. Scanning Near-field Optical Microscope (SNOM) images of the transmitted light after passing through the random Sb-SiN film were produced as well as a Finite Difference Time Domain (FDTD) calculation of the field distribution. They both showed a local optical near-field enhancement due to multiple scattering effects of the Sb nanoparticles. This is promising for application in superresolution optical storage to get a higher Carrier to Noise Ratio (CNR).

Elevated Expression of Nuclear Protein Kinase CK2α as a Poor Prognosis Indicator in Lymph Node Cancerous Metastases of Human Thyroid Cancers

Guo, Miao,Liu, Chao,Qi, Feng-Jie,Zhang, Xiu-Mei,Ren, Li-Li,Liu, Yi-Meng,Meng, Zhi-Chao,Zhu, Zhi-Tu,Xiao, Jian-Ying Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.17

Aim: To investigate the expression of protein kinase $CK2{\alpha}$ ($CK2{\alpha}$) in human thyroid disease and its relationship with thyroid cancer metastasis. Materials and Methods: Using immunohistochemistry we measured the expression of $CK2{\alpha}$ in 76 benign and malignant human thyroid cancer tissues, including 10 pairs of papillary carcinoma tissues with or without lymph node cancerous metastasis and similarly 10 pairs of lymph nodes. Results: The expression of $CK2{\alpha}$ was found to be higher in thyroid carcinoma cases (papillary carcinoma, follicular carcinoma, anaplastic carcinoma and medullary carcinoma) than in ones such as chronic lymphocytic thyroiditis, nodular goiter and adenoma. These findings were also confirmed by RT-PCR and Western blotting. More strikingly, elevated expression of $CK2{\alpha}$ in thyroid papillary carcinoma tissues was not only significantly associated with lymph node cancerous metastasis and clinical stage of thyroid cancers; but also correlated with epithelial-mesenchymal transition (EMT) and high tenascin C (TNC) expression. In addition, EMT and high TNC expression in thyroid carcinoma tissues was significantly associated with lymph node cancerous metastasis. Conclusions: Elevated expression of nuclear $CK2{\alpha}$ is a poor prognosis indicator in lymph node cancerous metastasis of human thyroid cancers.

Development of High-specificity Antibodies against Renal Urate Transporters Using Genetic Immunization

( Guo Shuang Xu ),( Xiang Mei Chen ),( Di Wu ),( Suo Zhu Shi ),( Jian Zhong Wang ),( Rui Ding ),( Quan Hong ),( Zhe Feng ),( Shu Peng Lin ),( Yang Lu ) 생화학분자생물학회 2006 BMB Reports Vol.39 No.6

Molecular Phylogenetic Diversity and Spatial Distribution of Bacterial Communities in Cooling Stage during Swine Manure Composting

Guo, Yan,Zhang, Jinliang,Yan, Yongfeng,Wu, Jian,Zhu, Nengwu,Deng, Changyan Asian Australasian Association of Animal Productio 2015 Animal Bioscience Vol.28 No.6

Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and subsequent sub-cloning and sequencing were used in this study to analyze the molecular phylogenetic diversity and spatial distribution of bacterial communities in different spatial locations during the cooling stage of composted swine manure. Total microbial DNA was extracted, and bacterial near full-length 16S rRNA genes were subsequently amplified, cloned, RFLP-screened, and sequenced. A total of 420 positive clones were classified by RFLP and near-full-length 16S rDNA sequences. Approximately 48 operational taxonomic units (OTUs) were found among 139 positive clones from the superstratum sample; 26 among 149 were from the middle-level sample and 35 among 132 were from the substrate sample. Thermobifida fusca was common in the superstratum layer of the pile. Some Bacillus spp. were remarkable in the middle-level layer, and Clostridium sp. was dominant in the substrate layer. Among 109 OTUs, 99 displayed homology with those in the GenBank database. Ten OTUs were not closely related to any known species. The superstratum sample had the highest microbial diversity, and different and distinct bacterial communities were detected in the three different layers. This study demonstrated the spatial characteristics of the microbial community distribution in the cooling stage of swine manure compost.

Bacillus ferrooxidans sp. nov., an iron(II)-oxidizing bacterium isolated from paddy soil

Guo-Wei Zhou,Xiao-Ru Yang,Jian-Qiang Su,Bang-Xiao Zheng,Yong-Guan Zhu 한국미생물학회 2018 The journal of microbiology Vol.56 No.7

An endospore-forming bacterium, designated YT-3T, was isolated from a paddy soil in Yingtan, Jiangxi, China. Cells of strain YT-3T were Gram-positive, rod-shaped, facultative anaerobic, catalase, and oxidase positive. The optimum growth temperature and pH were 30°C (ranged from 15 to 50°C) and 6.5–7.0 (ranged from 3 to 11), respectively. Analysis of the 16S rRNA gene sequence showed that strain YT-3T was affiliated to the genus Bacillus and displayed the highest similarity to that of Bacillus drentensis JCM 21707T (98.3%), followed by B. ginsengisoli JCM 17335T (97.8%) and B. fumarioli JCM 21708T (97.0%). The similarity of rpoB gene sequence between strain YT-3T and B. drentensis JCM 21707T, B. ginsengisoli JCM 17335T and B. fumarioli JCM 21708T was 80.4%, 81.5%, and 82.1%, respectively. The genomic DNA G + C content was 44.9 mol%. The predominant respiratory quinone was Menaquinone-7, and meso-diaminopimelic acid was present in the peptidoglycan layer of cell wall. The major fatty acids were C15:0 anteiso (36.2%), C14:0 iso (19.6%), C15:0 iso (17.4%), and C16:0 iso (9.8%). The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phospholipids, and ammoniac phospholipids. The DNA-DNA hybridization values between isolate YT-3T and B. drentensis (JCM 21707T), B. ginsengisoli (JCM 17335T), and B. fumarioli (JCM 21708T) were 36.3%, 30.3%, and 25.3%, respectively. On the basis of physiological, genetic and biochemical data, strain YT-3T represented a novel species of the genus Bacillus, for which the name Bacillus ferrooxidans sp. nov was proposed. The type strain is YT-3T (= KCTC 33875T = CCTCC AB 2017049T).

The Preparation and the Effects of K/Na Ratio on the Electrical Properties of 0.95(Na0.96−xKxLi0.04)(Nb0.89Sb0.07Ta0.04)O3-0.05KNbO3 Lead-free Ceramics

Jian Ma,Dingquan Xiao,Bo Wu,Jia-gang Wu,Jian-guo Zhu 한국물리학회 2014 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.65 No.2

0.95(Na0.96−xKxLi0.04)(Nb0.89Sb0.07Ta0.04)O3-0.05KNbO3 (abbreviated as KxNLNST-KN, x =0.32 − 0.52) lead-free piezoelectric ceramics were prepared by using the conventional solid-statesintering method, and the intentional addition of a pre-calcined KNbO3 powder was used as asintering aid that might inhibit and compensate for the volatilization of alkali metals during thesintering process. The preparation of the ceramics and the effects of K/Na ratio on the structureand the electrical properties of these ceramics were studied in detail. Compared with the relativelyhigher sintering temperature of Li-, Sb-, and Ta-modified KNN ceramics reported in the literature,the dense ceramic samples were obtained at a reduced temperature of 1060 C, and the ceramicswith x = 0.44 possessed optimal properties: d33 = 298 pC/N, kp = 48%, Pr = 21.9 μC/cm2, andEc = 11.5 kV/cm.

Experimental and numerical investigation of uplift behavior of umbrella-shaped ground anchor

Zhu, Hong-Hu,Mei, Guo-Xiong,Xu, Min,Liu, Yi,Yin, Jian-Hua Techno-Press 2014 Geomechanics & engineering Vol.7 No.2

In the past decade, different types of underreamed ground anchors have been developed for substructures requiring uplift resistance. This article introduces a new type of umbrella-shaped anchor. The uplift behavior of this ground anchor in clay is studied through a series of laboratory and field uplift tests. The test results show that the umbrella-shaped anchor has higher uplift capacity than conventional anchors. The failure mode of the umbrella-shaped anchor in a large embedment depth can be characterized by an arc failure surface and the dimension of the plastic zone depends on the anchor diameter. The anchor diameter and embedment depth have significant influence on the uplift behavior. A finite element model is established to simulate the pullout of the ground anchor. A parametric study using this model is conducted to study the effects of the elastic modulus, cohesion, and friction angle of soils on the load-displacement relationship of the ground anchor. It is found that the larger the elastic modulus and the shear strength parameters, the higher the uplift capacity of the ground anchor. It is suggested that in engineering design, the soil with stiffer modulus and higher shear strength should be selected as the bearing stratum of this type of anchor.

Characterization of the Open Reading Frame 35 of Bombyx mori Nucleopolyhedrovirus

Zhu, Ying Min,Li, Guo Hui,Yao, Qin,Chen, Ke Ping,Guo, Zhong Jian Korean Society of Sericultural Science 2010 International Journal of Industrial Entomology Vol.21 No.2

Open reading frame 35 (bm35) of the Bombyx mori nucleopolyhedrovirus (BmNPV) is a special gene whose homologues are only found in some group-I nucleopolyhedroviruses, suggesting that bm35 plays a specific role in the viral life cycle. This paper described the characterization of BmNPV bm35. Computerassisted sequence analysis shows that a putative RING finger motif is observed in the protein, Bm35 encoded by bm35. The coding sequence of bm35 was amplified and subcloned into the vector pET30a(+) and the $(His)_6$-tagged fusion protein His-Bm35 was expressed in the Escherichia coli BL21 (DE3) LysS cells. The bm35 transcript and Bm35 protein were detected in BmNPV-infected BmN cells at 12~48 h post infection (p.i.) by RT-PCR and Western blot analysis using the polyclonal antibody generated by immunizing a rabbit with purified $(His)_6$-tagged Bm35, suggesting that bm35 is synthesized in the late stage of BmNPV infection cycle. Bm35 was not a structural component associated with budded virus (BV) and occlusion derived virus (ODV). These data indicated that bm35 is a functional gene in the BmNPV life cycle.

Plasma Post-operative miR-21 Expression in the Prognosis of Gastric Cancers

Ma, Guo-Jian,Gu, Rong-Min,Zhu, Ming,Wen, Xu,Li, Jin-Tian,Zhang, Yuan-Ying,Zhang, Xiao-Mei,Chen, Sen-Qing Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.12

Tumor-associated microRNAs have been detected in serum or plasma, but whether plasma microRNA-21 (miR-21) could be a potential circulating biomarker for gastric cancer (GC) prognosis in Chinese is still uncertain. Real-time quantitative reverse transcription PCR (qRT-PCR) was employed in this study to compare the relative expression of miR-21 between pre-operative and post-operative paired plasmas from 42 patients with primary GCs. The results showed that the expression levels of miR-21 in the post-operative plasmas were significantly reduced by an average of 18.2 times in all patients when compared to the pre-operative plasmas, and by 22.1 times in the subgroup of patients without family history, while only 1.76 times in the subgroup of patients with a family history. With respect of clinicopathological characteristics, the plasma miR-21 expression was highly associated with differentiation degree and lymph node metastasis rate. The results suggested plasma miR-21 could be a novel potential biomarker for GC prognosis and evaluation of surgery outcomes, especially in patients without a family history.