배추 시스테인 단백질 분해 효소 억제 유전자 BCPI-1의 발현과 종자 발아 및 유묘 생장과의 연관성

홍준기,이은영,김정률,양경애,최영주,정우식,김호일,윤대진,이상열,조무제,임체오 Plant molecular biology and biotechnology research 2003 Plant molecular biology and biotechnology research Vol.2003 No.-

Phytocystatins are protein inhibitors of cysteine proteinases of the papain family that have been identified in both monocot and dicot plants. A cDNA encoding a phytocystatin, BCPI-1 (Brassica Cysteine Proteinase Inhibitor-1) has been isolated from Chinese cabbage (Brassica campestris L. ssp. pekinensis) flower buds. Here, we tested whether BCPI-1 transcription is regulated by hormones, and could be involved in regulating cysteine proteinases during and after germination. BCPI-1 was sensitive to exogenous GA_(3) and ABA, which are important factors controlling seed germination, suggesting that the expression of BCPI-1 is hormonally regulated. We introduced a recombinant plasmid containing the full-length BCPI-1 cDNA under the control of cauliflower mosaic virus 35S promoter into rice embryogenic calli using the particle delivery method, and regenerated a number of transgenic rice plants. Constitutively over-expressed BCPI-1 caused changes in overall plant growth and development, including reduced germination and seedling growth. These data support the role of the BCPI-1 in the regulation of endogenous proteinases during both seed germination and subsequent seedling development. Phylocystatins은 papain계열의 cysteine 단백질 분해 효소 활성을 특이적으로 억제하는 억제자로 다양한 식물 종으로부터 분리되었다. 본고에서는, 배추 화아 cDNA library로부터 분리된 phytocystatin인 BCPI-1(Brassica Cysteine Proteinase Inhibitor-1)의 생체 내 기능에 대해 연구하였다. 먼저, BCPI-1 전사체는 발아 조절에 중요한 영향을 미치는 GA₃와 ABA에 의해 예민하게 증가, 혹은 감소되는 반응을 보임으로써, BCPI-1이 식물 호르몬의 영향을 받으며, 특히 발아나 유묘의 생장 조절에 관련이 있음을 알 수 있었다. Particle bombardment 방법을 통하여 BCPI-1을 벼의 배 형성 세포 내에 도입, 재분화 시켜 형질 전환 벼를 생성하였다. CaMV 35S promoter의 조절에 의해 지속적인 BCPI-1 발현을 보이는 형질 전환 벼의 경우, 발아와 유묘 생장이 현저히 지연되었다. 위의 결과들을 바탕으로, BCPI-1이 식물체 내에서 생성되는 cysteine 계열의 단밸질 분해 효소 활성을 조절하여 종자의 발아와 유묘의 생장에 영향을 미치는 것으로 추측한다.

고속 주축 상태 모니터링용 광파이버 변위 센서 설계 제작 및 성능평가

홍준희,박찬규,신우철,이동주 한국공작기계학회 2004 한국공작기계학회 추계학술대회논문집 Vol.2004 No.-

This paper is focused on practical applicability of the optical fiber sensor considering the machine center which is going to use them. Optical fibers may be fluctuated because the machine center operates as column moving type. This causes distortion of the sensor output signal. To reduce this problem, we have improved the sensor structure and its bracket. And we evaluated performances of the sensor.

합성섬유를 혼입한 콘크리트의 강도 및 내구특성

홍창우,정영화,이주형,이정호 江原大學校 産業技術硏究所 1998 産業技術硏究 Vol.18 No.-

Concrete structures has been deteriorated by poor environment. This study was conducted to evaluate durability of concrete which are increasingly demanded recently. Therefore, the research of durability must be executed for application of Polypropylene fiber reinforced concrete real structures. Concrete durability properties incorporating Polypropylene fiber was performed with the variable of Fiber contents, Fiber type and Target strength, specimens were made and subjected to durability and strength tests. The results show that strength of concrete is increased the Fiber content increase, Mono-Filament fiber and Polypropylene fiber reinforced concrete makes improved durability properties.

유도형 변위 센서의 전기 노이즈 분석과 센서 성능에 미치는 영향 고찰

신우철,홍준희,이동주 한국공작기계학회 2003 한국공작기계학회 추계학술대회논문집 Vol.2003 No.-

Noise is a problem in many electronic circuits and active control system. Arising from spuriously coupled noise from other circuits, it corrupts the signal of interest and introduces an uncertainty into information it contains. In this paper, we have researched noise characteristics of the inductive displacement sensor which has been designed. we first present basic concept and characteristics of magnetic field-coupled noise in the sensor output signal. Then, we are present relation noise and sensor performances. Finally, we concentrate low noise design of a sensor driver and a signal detection circuit.

구속된 비행 청소년들의 특성 및 석방 후 6개월간 재범여부와 관련된 변인

정선주,김원식,고승희,구영진,김홍창,서동혁 대한소아ㆍ청소년정신의학회 1999 소아청소년정신의학 Vol.10 No.2

식품 알러지 억제를 위한 감마선 조사기술의 적용

변명우,이주운,육홍선 경산대학교 생명자원개발연구소 2001 생명자원과 산업 Vol.5 No.-

This study was carried out to evaluate the application of food irradiation technology as a method for reducing food allergy. Bovine α-casein and β-lactoglobulin, hen's egg albumin(ovalbumin), an egg white protein, and a heat stable protein of shrimp, tropomyosin were used as model allergens and was gamma-irradiated in an aqueous state. The changes in allergenic properties of these allergens resulted from gamma irradiation were monitored by Ci-ELISA with serum from each target food-hypersensitive patients. The results provide a new possibility to use irradiation process for reducing food allergy.

新農村建設(定住生活圈開發)을 위한 理論的 考察 및 開發需要調査

金鴻允,李弘柱,鄭禮杓,全禹亭,趙興洙,李信昊,全榮吉 안성산업대학교 1992 論文集 Vol.24 No.-

Rural of Koreas has rapidly changed through the process of industrialization and urbanization. During past 30 years, development differences between urban and rural, metroplitan area and area derived from a highly economic growth, bring about dual specialization that deepens overpopulation and sparsity. Therefore, with a new concern about rural, necessity of rural development policy that developes a settlement and life area for settlement for ensuing stablized settlement and living activity of rural people, has been drived step by step. For rural welfare establishment that is settled and good hereafter, this study aims at attempting a theoretical approach about the way of development for rural settlement as a new rural development strategy through examination of today's rural reality, under assumption that rural people must be able to obtain stable jobs and to be pleasant without leaving rural area, and rural settlement to be able to enjoy urban advantages must be realized. Therefore, items that must be obtained basically rural area to be a human settlement space are equipment connected with a dwelling house, production, consumption, job relaxation, infrastructure connected with transportation or communication network and environment. By the way, the best function of a human settlement space makes a basic pleasant settlement area to be in the middle of that. Then rationalization of land use must be realized as being a best condition for human life around space of a dewelling house. Besides, spacial accessibility about all equipment of inhabitants must be easy. That is, economic distance, social distance and cultural distance as well as spacial distance must be included. Also, those spacial functions must be realized on a basis of suitable enironment for a human settlement life. At the same tiem, the upper area must be connected with the lower area as the system of spacial settlement in settlement space. But truely, as rural people can't satisfy with services of the surrounding rural areas, they desire better city or metroplitan. The these problems bring about inequality between urban and rural. Therefore, here are urgent reasons that must be done rearing of rural central town and rural integrated development of unit area. So, these dys, to correct maldistribution of land use and inequality of settlement space that Korean society faces on, new strategies of rural integrated development for lift area for settlement than gives the meaning. That rural areas become like a place for human settlement life will be able to be chosen in the view of humanism. The say of thinking of rural residents(Jukans-myun, Anseong-gun, Kyeonggi-do) was surveyed in order to develop new rural settlement planning. From the data collected in this survey, rural life-style, view on traditional rural life, agriculture, farming, agri-policy, social attitude, and degree of satifaction of rural life, etc, were analyzed. Firstly, most of rural residents interviewed in this survey have the opinion of not leaving their home village if living conditions improved to a reasonable level in the near future. In decision-making process of village affairs, they want to participate actively and equally. Secondly, in spite of their acceptance of agriculture as a vital sector destined to national security, most of them think that framing should be little of promise. Thirdly, they have the negative attitude to urban-oriented social valued and also to the agricultural policy, while seeking consolation through human and traditional viewing of rural life style. Therefore, in the development of new rural settlement model, idea-making should be directed to the betterment of communal value of village life, stability of rural economy, rationalization of rural life style and enhancement of rural-type happiness.

직장에 발생한 원발성 악성 흑색종 1예

이석주,김광일,김동호,김홍성,우상민,최덕례,정현용 충남대학교 의과대학 지역사회의학연구소 1997 충남의대잡지 Vol.24 No.1

An extremely rare case of primary melanoma involving only rectum is reported. This 59 year-old male patient was admitted to the hospital because of hematochezia, difficulty of defection. Endoscopic examination and radical excision showed polypoid lesion with ulceration which measured 6x6x3cm in 2cm from the anal verge. Histopathologic, H&E stain and electron microscopic examination(EM) were performed from endoscopic biopsies and excisional mass. We are reporting a case primary rectal melanoma with brief review of literature.

다량의 하부위장관출혈이 병발된 Vibrio vulnificus 패혈증 1예

최주연,유진홍,한석원,이대훈,최민호,박순민,김연식,김선우,신완식,강문원,강성구 대한화학요법학회 1996 대한화학요법학회지 Vol.14 No.2

최근 저자들은 Vibrio vulnificus 감염에 의해 패혈증 및 이차성 피부 병변을 보인 42세의 남자 환자의 치료중 하부 위장관 출혈이 병발된 1예를 경험하였기에 보고하는 바이다. We experienced a case of Vibrop vulnificus septicemia which showed an unusual manifestation such as hematochezia. The patient was a 42-year-old man with chronic liver disease. The blood and wound culture revealed the organism. During intensive care, he suffered from massive hematochezi for several days. Colonoscopy and abdominal CT showed findings suspicious of ischemic colitis. To our knowledge, this is the first unusual case of V.vulnificus infection which showed massive lower gastrointestinal bleeding.

재발성 일과성 허혈 발작을 주소로 내원한 비-안와-대뇌 모균증 1예

강우열,이주헌,성영희,최일수,송홍기,이병철 대한노인병학회 2002 대한노인병학회지 Vol.6 No.3