복어중독에 의한 가사 상태에서 소생한 1예

송승찬,신진호,강석우,박경남,최호순,박근태,문희식,기춘석,이성희,윤병철,노우균,조균석,이민호 大韓應急醫學會 1998 대한응급의학회지 Vol.9 No.3

Tetrodotoxin is a neurotoxin produced by about 90 species of puffer fish and causes paralysis of central nervous system and peripheral nerves by blocking the movement of all monovalent cation. Ingestion of tetrodotoxin produces clinical manifestations such as paresthesias(within 10-45 min), vomiting, lightheadedness, salivation, muscle twitching, dysphagia, difficulty in speaking, convulsion and death that expressed by cardiopulmonary arrest with loss of brain stem reflex sometimes. Tetrodotoxin prevents or delays ischemia induced neuronal death by way of following 3 mechanisms. Firstly, it reduces the energy demand of the brain tissues. Secondly, it delays or even prevents anoxic depolarization. Finally, it deminishes ischemia induced cell swelling and cerebral edema. We report a case of puffer fish poisoning which presented with cardiopulmonary arrest and loss of brain stem reflex, but completely recovered by aggressive cardiopulmonary resuscitation.

요추 척추관 협착증 환자의 적외선 체열상

남상범,이호동,이윤우 대한체열학회 2003 대한체열진단학회지 Vol.3 No.1

mecA 양성 메티실린 내성 포도구균의 mec 조절유전자에 대한 분자역학적 연구 및 다양성 분석

우희연,이남용,맹성호,한승훈,인경수,김상욱,성승용,김익상,최명식 대한화학요법학회 2000 대한화학요법학회지 Vol.18 No.3

목적 : 병원 감염과 지역 감염의 원인균으로서의 중요성이 증가되고 있는 MRSA와 MRCNS의 메티실린 내성 관련 유전자에 대해 분자역학적인 조사를 시행하고 mec 조절유전자의 다양성을 분석하여 분자유전학적 연구와 감염 관리에 기본적 자료를 제공하고자 연구를 시행하였다. 방법 : 본 연구에서는 국내 동일 대학병원에서 분리된 mecA 양성 MRSA 78균주와 MRCNS 36균주를 대상으로 항균제 감수성 검사와 중합효소연쇄반응을 시행하여 표현형과 유전형의 분포를 알아보고 내성 정도와 유전형의 상관성을 분석하였다. 결론 : 최소억제농도를 측정하여 oxacillin 감수성 양상을 알아본 결과 총 90개의 MRSA 임상검체 중 3균주에서 감수성을 보였고, MRCNS 36균주는 모두 내성을 보였으며, 고도 내성을 보이는 균주일수록 다제내성을 보이고 있었다. 중합효소연쇄반응으로 mecA 유전자 유무를 분석하여 mecA 양성을 보인 MRSA 78균주와 MSCNS 36균주 만을 대상으로 mec 조절유전자에 대한 중합효소연쇄반응을 시행하여 유전형의 분포를 조사하였다. 그 결과 MRSA와 MRCNS 모두에서 mecA-Pr-mecA-mecR1-mecI형, mecA-Pr-mecA-mecR1형과 mecA-Pr-mecA-mecR-1의 5′말단형의 3가지 유전형이 공통적으로 관찰되었는데, 이외에 MRSA에서는 mecA-mecR1형, mecA-mecR1의 5′말단형이, MRCNS에서는 mecA-Pr-mecA형과 mecA형이 추가로 분리되었다. 유전형의 분포를 연도별로 분석한 결과 연도별로 우세한 유전형이 서로 다른 것을 알 수 있었다. 유전형과 내성 정도의 상관성을 분석한 결과, mecA-Pr-mecA-mecR1을 보유한 MRSA의 경우 mecA 억제유전자로 생각되는 mecI 유전자 유무에 관계없이 모두 고도 내성을 나타내어 mecI의 유무는 내성 정도와는 일정한 관련이 없었으나, MRSA와 MRCNS 모두에서 mecI와 동시에 mecR1의 3′말단이 결혼된 유전형이 저도 및 중등도 내성을 보이는 균주에서 유의하게 많이 관찰되어 mecI가 결손된 균주에서는 mecR1의 3′말단부위의 유무가 내성 정도의 결정에 중요한 역할을 할 것으로 생각하였다. 결론 : 국내에서 분리되는 메티실린 내성 포도구균의 유전형의 분포가 국외 분리주와 차이를 보이는 것으로 관찰되었다. 또한 기존의 보고와는 달리 메티실린 내성 정도와 유전형 사이에 유의한 연관성이 있는 것으로 관찰되어 mec조절유전자가 내성 발현 뿐만 아니라 내성 정도의 결정에 중요한 역할을 하는 것으로 관찰되었다. MRSA가 중요한 감염균임에도 불구하고 아직까지 MRSA의 내성기전에 대해 확실하게 규명되지 않아 치료가 더욱 어려워지고 있는 실정이므로 이에 대한 더욱 종합적인 연구와 동시에 발생과 확산을 막기 위한 노력이 필요할 것으로 생각된다. Background : High prevalence of methicillin resistance has been noticed in staphylococci which also have been recognized as important nosocomial and multi-drug resistant pathogens. In this study, we investigated the distribution of mec regulator genes and the presence of the mutation in mecIgene to reveal the resistance mechanism at molecular level. Methods : The isolates included 90 clinical isolates of MRSA, 36 MRCNS of which methicillin resistance were determined by disk diffusion test and isolated in a single hospital during 1996-1999. We performed microdilution MIC test for oxacillin resistance and Kirby-Bauer test for other antibiotics. Genotypes of mecA positive (determined by PCR) isolates (78 MRSA, 36 MRCNS) were investigated by PCR amplification of mec regulator genes such as 3' end and 5' end of mecR1. mecI, and Pr-mecA. Results : In MIC assay, all MRSA strains except 3 strains and all MRCNS strains showed oxacillin resistance. The level of resistance correlated to the multi-drug resistance. Seven genotypes were observed in mecA positive MRSA and MRCNS. Differences of predominant genotypes among years isolated and among species were observed. Deletions of the 3' end of mecR1 and the mecI were more frequently observed in the low- and borderline-level resistant MRSA and MRCNS (P(0.05). So the important role of 3' end of mecRI in determining resistance level was suggested. Conclusion : This study demonstrated that genomic distribution of methicillin-resistant staphylococci isolated in Korea was different from that of strains isolated in other countries. And important role of mec regulator genes for expression of methicillin resistance was suggested.

가스크로마토그래피-이온트랩질량분석법을 이용한 하수슬러지 중 다핵방향족탄화수소(PAHs) 함량 분석

남재작,소규호,박우균,조남준,이상학 한국환경과학회 2002 한국환경과학회지 Vol.11 No.4

The polycyclic aromatic hydrocarbons(PAHs) content in sewage sludge was determined by gas chromatography linked to ion trap mass spectrometry(GC/ITMS) with five deuterated PAHs as internal standards. The minimum detection limit was from 1.66 to 7.14 pg for individual PAH by GC/ITMS. For determining total PAHs(ΣPAH) in sewage sludge 84 samples from 74 waste water treatment plants in whole country were analyzed. The average content of ΣPAH for whole samples was 3,289±3,098 ㎍/kg, and ranged from 142 to maximum 20,102 ㎍/kg. According to the number of population of the city, the areas were classified as five regions, ie. big, large, middle, small, and rural area in which the waste water treated plants were grown. The contents of PAHs were 4,689±5,503, 5,839±6,401, 3,725±2,053, 2,237±2,069, and 2,475±1,489 ㎍/kg, in big, large, middle, small, and rural area, respectively.

페놀베타인 유도체합성(XI)

우성주,박예진,황순호,홍유화,김남재,김인종,김신규 慶熙大學校 1996 論文集 Vol.25 No.-

Tetrahydroberberine-13-one was treated with ethylchloroformate to give the C_8-N bond cleaved product 1. On the treatment with Lawesson's reagent (LAS), chloroketone 2 derived from the hydrolysis of 1 afforded the thioketone 3. On the other hand, spirotype-chloroketone 4 was transformed to diaziridine 8 by a reduction, chlorination and aziridine introduction. Also, spirotype-chlorothioketone 6 was obtained from chloroketone 4.

B_(2)O_(3)첨가에의한 LNT(Li_(1/2)Nd_(1/2)TiO_(3))-CT(CaTiO_(3)) 세라믹의 저온 소결 및 마이크로파 유전특성

이찬우,최명호,김남철 公州大學校 工學硏究院 生産技術硏究所 2004 論文集 Vol.12 No.-

The effect of B_(2)O_(3) addition on the microstructures and the microwave dielectric properties of 0.5LNT(Li_(1/2)Nd_(1/2)TiO_(3)) - 0.5CT(CaTiO_(3)) Ceramics have been investigated. It is found that B_(2)O_(3)(up to 5wt%) can significantly improve the density and dielectric properties of 0.5LNT(Li_(1/2)Nd_(1/2)TiO_(3)) - 0.5CT(CaTiO_(3)) Ceramics. 0.5LNT-0.5CT Ceramics with additives could be sintered to a density higher then 95% at 900℃, due to the liquid phase effect of B_(2)O_(3) addition, as observed by Scanning electron microscopy(SEM). A secondary pahse was not observed at the level of 5 ~ 10wt% B_(2)O_(3) addition. The microwave dielectric properties of 0.5LNT(Li_(1/2)Nd_(1/2)TiO_(3)) - 0.5CT(CaTiO_(3)) with 5wt%B_(2)O_(3) were as follows : ε_(r) = 85, Qxf_(0) = 2,500 and Tcf = -9ppm/℃, sintered at 900℃/2hr.

순무(Brassica campestris ssp. rapa) 뿌리로부터 지질화합물의 분리 및 동정

방면호,오영준,유종수,한민우,정해곤,정태숙,최명숙,이경태,김세영,백남인 경희대학교식량자원개발연구소 2007 硏究論文集 Vol.26 No.1

순무 뿌리로부터 활성 물질을 분리 동정 하기 위하여 80% MeOH 수용액으로 추출하고 이를 여과, 감압 농축하여 MeOH추출물을 얻었다. 이를 EtOAc분획, n-BuOH분획, H_(2)O분획으로 나누었으며, EtOAc분획에 대해 silica gel column chromatography를 실시하여 3종의 지질화합물을 분리 정제하였다. ^(1)H-NMR, ^(13)C-NMR, DEPT spectrum 및 Mass spectrum등을 통하여 palmitic acid methyl ester(compound 1), linolenic acid methyl ester(compound 2), linoleic acid methyl ester(compound 3)으로 구조를 결정하였다. In order to isolate and identify the active compound from the Brassica campestris ssp rapa, the roots and the root skin were extracted with 80% aqueous MeOH, and the concentrated extract was partitioned with EtOAc, n-BuOH and H_(2)O. From the EtOAc fraction, three compounds were isolated through the repeated silica gel column chromatographies. From the result of spectral data including NMR and MS, the chemical structures of the compound were determined as palmitic acid methyl ester (compound 1), linolenic acid methyl ester (compound 2), linoleic acid methyl ester (compound 3).

복합레진의 중합 수축력과 수복물의 결합양상에 관한 연구 제 2 편 결합강도

박남수,우이형,최부병,임호남,최대균 大韓齒科器材學會 1993 대한치과재료학회지 Vol.20 No.2

Failure of bond between resinous restorative materials and tooth can be occured by the polymerization contraction forces larger than bond strengths. This study was planned to confirmed that the bond strengths between composite resin and acid etched enamel will be influenced by the polymerization contraction forces altered with filler contents and diameter of attached resin specimens. Ten kinds of experimental composite resins were prepared and bonded to bovine tooth enamel which were acid-etched. The diameter of resin specimens attached were 4 mm and 6 mm. The following conclusions were drawn : 1. Shear bond strengths were increased and then decreased with rise of filler contents, maximum shear bond strength was obtained by the 40% filled composite resin. 2. Unit shear bond strengths of resin specimens bonded with large area were lower than that of narrow area bonding. It was concluded that shear bond strengths to acid etched enamel can be influenced by the polymerization contraction forces.

복합레진의 중합 수축력과 수복물의 결합양상에 관한 연구 제 1 편 : 중합수축력

박남수,최대균,임호남,최부병,우이형 大韓齒科器材學會 1993 대한치과재료학회지 Vol.20 No.1

The polymerization contraction forces has a relationship with the contents of filler, and if forces surpass the bond strength between restoration and bonded surface, the failure of bond can be occured. This study was designed to evaluate the effects of filler contents on the polymerization contraction forces and to confirm whether the polymerization forces could induce the bond failure of composite resins to enamel or dentin. Low capacity load cell using strain gauge were prepared and used as a measurement system for polymerization contraction forces. Oxidized alloy surfaces, enamel surfaces and dentin surfaces were used as bonded surface. Measurements of the polymerization contraction forces were done for 1 hour, and a stress at 1 hour after was recorded as a polymerization contraction force. It was considered as a bond failure that the polymerization contraction forces increased and then decreased by the time. The following conclusions were drawn : 1. Polymerization contraction forces of composite resins were decreased by the increase of filler contents. 2. Polymerization contraction forces were increased at thickened resin specimens, but it was not significant at over two millimeter thickness. 3. Bond strength of composite resins to the acid etched enamel surfaces exceeded the polymerization contraction forces and the reduced polymerization contraction forces due to the bond failure were not confirmed. 4. Failure of bond and reduced polymerization contraction forces occured on dentin.

SM45C강의 고온피로에 미치는 Notch효과에 관한 실험적 연구

崔壯宇,金楠鎬 弘益大學校 1987 弘大論叢 Vol.19 No.2

This paper the Rotating bending fatigue tests were performed on the SM45C carbon steel, and the effect of the test temperature on the fatigue strength, the notch factor and the shape of fatigue fracture surface were studied. The following results are obtained: 1. In case of test temperature 300°C, the fatigue strength of smooth specimen, U-notched specimen, V-notched specimen were denoted by 10^(7) Cycle increased 16.5%, 14.7%, 10.1% as compared with fatigue strength of room temperature. 2. A difference of the fatigue strength of smooth specimen was great in each temperature of the room temperature and 300°C, 450°C, but that was decreased of notched specimen as increased of the revolution cycles. 3. According as stress concentration factor being increased, the notch factor was increased. It was a greater notch factor than the stress concentration factor, when the range of N>1 x 10^(6) at 300°C_in U-notched specimen, and when the range of N>6 x 10^(6) at 450°C in U-notched specimen. 4. Variation of notch factor by the revolution cycles, according as the revolution cycles being increased, the notch factor was increased when the room temperature, 300°C, but notch factor takes constant value at 450°C. In case of stress concentration factor 1.87, notch factor was almost equal to the stress concentration factor. 5. When the approximate equation of Peterson and Neuber by notch factor applied an experimental results, approximate equation of Peterson approached an experimental results as inside of 25% error. 6. The fracture surface in notched specimen had the difference of the location and the area of brittle fracture surface in compliance with height of stress level.