Exosomes derived from miR-214-3p overexpressing mesenchymal stem cells promote myocardial repair

Wenwu Zhu,Qingjie Wang,Jian Zhang,Ling Sun,Xiu Hong,Wei Du,Rui Duan,Jianguang Jiang,Yuan Ji,Haoran Wang,Bing Han 한국생체재료학회 2023 생체재료학회지 Vol.27 No.00

Aims Exosomes are known as nanovesicles that are naturally secreted, playing an essential role in stem-mediated cardioprotection. This study mainly focused on investigating if exosomes derived from miR-214 overexpressing mesenchymal stem cells (MSCs) show more valid cardioprotective ability in a rat model of acute myocardial infarction (AMI) and its potential mechanisms. Methods Exosomes were isolated from control MSCs (Ctrl-Exo) and miR-214 overexpressing MSCs (miR-214OE-Exo) and then they were delivered to cardiomyocytes and endothelial cells in vitro under hypoxia and serum deprivation (H/SD) condition or in vivo in an acutely infarcted Sprague-Dawley rat heart. Regulated genes and signal pathways by miR-214OE-Exo treatment were explored using western blot analysis and luciferase assay. Results in vitro , miR-214OE-Exo enhanced migration, tube-like formation in endothelial cells. In addition, miR-214OE-Exo ameliorated the survival of cardiomyocytes under H/SD. In the rat AMI model, compared to Ctrl-Exo, miR-214OE-Exo reduced myocardial apoptosis, and therefore reduced infarct size and improved cardiac function. Besides, miR-214OE-Exo accelerated angiogenesis in peri-infarct region. Mechanistically, we identified that exosomal miR-214-3p promoted cardiac repair via targeting PTEN and activating p-AKT signal pathway. Conclusion Exosomes derived from miR-214 overexpressing MSCs have greatly strengthened the therapeutic efficacy for treatment of AMI by promoting cardiomyocyte survival and endothelial cell function.

Cloud Computing Environments Parallel Data Mining Policy Research

Wenwu Lian,Xiaoshu Zhu,Jie Zhang,Shangfang Li 보안공학연구지원센터 2015 International Journal of Grid and Distributed Comp Vol.8 No.4

Deep Learning-Based Modulation Detection for NOMA Systems

( Wenwu Xie ),( Jian Xiao ),( Jinxia Yang ),( Ji Wang ),( Xin Peng ),( Chao Yu ),( Peng Zhu ) 한국인터넷정보학회 2021 KSII Transactions on Internet and Information Syst Vol.15 No.2

Since the signal with strong power need be demodulated first for successive interference cancellation (SIC) receiver in non-orthogonal multiple access (NOMA) systems, the base station (BS) need inform the near user terminal (UT), which has allocated higher power, of the far UT’s modulation mode. To avoid unnecessary signaling overhead of control channel, a blind detection algorithm of NOMA signal modulation mode is designed in this paper. Taking the joint constellation density diagrams of NOMA signal as the detection features, the deep residual network is built for classification, so as to detect the modulation mode of NOMA signal. In view of the fact that the joint constellation diagrams are easily polluted by high intensity noise and lose their real distribution pattern, the wavelet denoising method is adopted to improve the quality of constellations. The simulation results represent that the proposed algorithm can achieve satisfactory detection accuracy in NOMA systems. In addition, the factors affecting the recognition performance are also verified and analyzed.

Identification and expression profiling of putative chemosensory protein genes in two rice planthoppers, Laodelphax striatellus (Fallén) and Sogatella furcifera (Horváth)

Wenwu Zhou,Xin Yuan,Ping Qian,Jiaan Cheng,Chuanxi Zhang,Geoff Gurr,Zengrong Zhu 한국응용곤충학회 2015 Journal of Asia-Pacific Entomology Vol.18 No.4

Chemosensory proteins (CSP) afford insects the ability to recognize complicated environmental cues and are involved in diverse physiological processes. The planthoppers Laodelphax striatellus and Sogatella furcifera are serious specialist pests of rice throughout eastern Asia. In this study, we identified 21 putative CSP genes (namely LsCSP1–12 and SfCSP1–9) in the two planthoppers L. striatellus and S. furcifera, and profiled their transcript accumulation in different developmental stages and body parts by real-time quantitative PCR (qPCR). All CSPs had the characteristic typical features of the CSP family, with the exception of LsCSP10 and SfCSP2, which lacked the predicted signal peptide sequence. Among these putative CSP genes, only two (LsCSP9 and SfCSP5)weremore highly expressed in the antennae, indicating that they might be involved in olfaction. On the contrary, eleven genes (LsCSP2, 3, 4, 6, 8, 12; SfCSP1, 4, 6, 7, 8) and one gene (SfCSP9) were more highly expressed in the legs and heads (without antennae), respectively. The remainders of the genes were ubiquitously expressed in the heads (without antennae) and the bodies (without the legs and heads). The broad expression patterns in different body parts indicate that the CSPs of these two delphacid species may be involved in diverse functions besides chemosensation. These findings increase the understanding of the chemosensory protein system of Hemiptera species.

Influence of water-stressed rice on feeding behavior of brown planthopper, Nilaparvata lugens (Stål)

Ye Tan,Mufei Zhu,Wenyan Xu,Wenwu Zhou,Dongdong Lu,Hanwu Shang,Zengrong Zhu 한국응용곤충학회 2017 Journal of Asia-Pacific Entomology Vol.20 No.2

Climate change can impact insects through abnormal weather conditions such as elevated temperatures and droughts. Crops experiencing periods of water stress from droughts may have significant effects on pest populations because of physiology changes, insect behavior and adaptation. Herewe report on the effects ofwater stress on the rice pest, the brown planthopper (BPH), Nilaparvata lugens (Stål). In themultiple choice test, both the fifth instar nymphs and female adults BPH preferred plants under the low stress level over control and high stress plants. The electrical penetration graph (EPG) showed that the fifth instar nymphs spent more time in non-penetration activities while the female adults spent less. Both the fifth instar nymphs and female adults spent more time in salivation on high stress plants than in control and low stress plants indicating difficulties in feeding. In addition there was marked increase in probe numbers made by both the fifth instar nymphs and the female adults in the feeding duration. The fifth instar nymphs made twice as many numbers of probes than those in the control (9.36 vs to 5.25 min) while the female adults made about 4 times more probes (13.13 vs 3.00 min). Both the fifth instar nymphs and the female adults producedmore honeydewon the lowstress plants suggesting that plants in low water stress favored insect feeding. These findings indicate that BPH in low water stress rice plants seem to do better which supports the theory that drought induces pest outbreaks.

Reliable Mobility Evaluation of Organic Field-Effect Transistors With Different Contact Metals

Huang, Fanming,Liu, Ao,Zhu, Huihui,Xu, Yong,Balestra, Francis,Ghibaudo, Gerard,Noh, Yong-Young,Chu, Junhao,Li, Wenwu IEEE 2019 IEEE electron device letters Vol.40 No.4

<P>The reliability of mobility evaluation of organic field-effect transistors (OFETs) is a serious issue because numerous overestimated and underestimated mobilities have been reported recently. A reliable approach to accurately evaluate the OFET mobility is therefore highly desirable. Here, in this letter, two commonly employed methods and the Y function method (YFM) were used to extract the mobilities of indacenodithiophene-co-benzothiadiazole (IDT-BT) OFETs with four different contact metals. The mobilities extracted by the commonly used methods varied greatly with the contact metal and channel length, whereas those extracted by the YFM were very stable and approached the intrinsic mobility of IDT-BT. Our results show that YFM is a precise approach that can be widely used to evaluate the OFET mobility.</P>

Multi-objective optimization design of a centrifugal impeller by positioning splitters using GMDH, NSGA-Ⅲ and entropy weight-TOPSIS

Xing Xie,Zhenlin Li,Baoshan Zhu,Hong Wang,Wenwu Zhang 대한기계학회 2021 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.35 No.5

A centrifugal impeller with splitters was designed by three-dimensional (3D) inverse design method, and its efficiency, velocity non-uniformity at impeller exit and maximum equivalent stress of blades were optimized by providing the suitable blade stacking angle, work ratio and circumferential location of the splitter. First, 80 samples were generated by optimal Latin hypercube technique and the corresponding impellers were designed by 3D inverse design. Using computational fluid dynamics (CFD) and fluid structure interaction (FSI), the optimization objectives were obtained. Then, the group method of data handling (GMDH) artificial neural networks was established to link the design parameters and objectives by the specific formulas. The reference-point-based non-dominated sorting genetic algorithm (NSGA-Ⅲ) was applied to search the Pareto front. Finally, the preferred impeller was selected by adopting entropy weight and the method of technique for order preference by similarity to an ideal solution (TOPSIS). The results showed that the splitter of the preferred impeller had a circumferential location of 0.58, blade stacking angle of 28° and work ratio of 0.48. The nonuniformity at impeller exit and maximum equivalent stress of blades of preferred impeller obtained by NSGA-Ⅲ were decreased, while the efficiency was improved.

Parameter Sensitivity Study on Static and Dynamic Mechanical Properties of the Spatial Y-shaped Tied Arch Bridge

Jianpeng Sun,Zihan Tan,Jiaju Zhang,Wenwu Sun,Li Zhu 한국강구조학회 2023 International Journal of Steel Structures Vol.23 No.2

The spatial Y-shaped tied arch bridge is a rare form of innovative bridge on arch bridges around the world. It has important reference significance for the design and construction of bridge engineering worldwide. This arch bridge is novel in design and adopts single and double arch ribs combined structure. However, as a novel bridge type, its force situation is indetermination, so it is very important to study its mechanical properties and parameter sensitivity. In order to study the mechanical properties of the spatial Y-shaped tied arch bridge and the influence of structural parameters, this paper takes a spatial Y-shaped tied arch bridge under construction in China as the research object. The finite element software MIDAS Civil is used to establish the bridge model. The finite element model is used to analyze the static and dynamic performance of the spatial Y-shaped tied arch bridge under the use stage and the mechanical change trend under the influence of different rise-span ratios and double arch bifurcation angles. The results of single arch rib and double arch rib under constant load and live load are compared under different structural parameters in this paper, and the parameter sensitivity analysis of statics and dynamics is carried out. These analyses provide the adjustment basis and design reference for the design of the special-shaped arch bridge in the future.