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Design for Ontology Knowledge Base Based on Structural Members
Qian Li-Ping,Yang Xiao-Ping,Song Yu 보안공학연구지원센터 2015 International Journal of Database Theory and Appli Vol.8 No.5
By studying the existing ontology design method and integrating the feature of domain knowledge which has significant component characteristics, this paper propose a method to build ontology knowledge base basing on structural member. The process includes the following six steps: requirement analysis, framework designing, coding, ontology evaluation, ontology evolution, document filing. Each step of the task is further decomposed into several detailed operation. An ontology evaluation method is also proposed. Through applying the domain ontology into the practices and evaluating the result before and after, the rationality of relationship is reversely inferred out. Ontology language is used to describe the concepts and the relation among them. It is also an ideal selection to describe the ancient buildings knowledge. The result of instant study confirms the approach is feasible and effective.
Ping Qian,Xin Wang,Tao Jianga,Fei Song,Chen Chen,Yangyang Fan,Xing-jia Shen 한국응용곤충학회 2016 Journal of Asia-Pacific Entomology Vol.19 No.2
MicroRNAs (miRNAs) are a class of endogenous, non-coding small RNAs that serve as important posttranscriptional gene expression regulators and play important roles in the silkworm (Bombyx mori) development, growth, and viral immunity. However, information on the diversity of these regulatory RNAs in the middle silk gland (MSG) of naked pupa (Nd) mutant silkworms is limited. In this study, by using Solexa high-throughput sequencing technology, we identified and compared small RNA libraries from the MSG of wild-type silkworm P50(MSG-P50) and the Nd mutant (MSG-Nd), respectively. A total of 272 conserved and 333 novel miRNAs were identified, in which 141 ones showed significantly different expression patterns between MSG-P50 and MSG-Nd, and 10 ones were randomly selected and validated by stem-loop quantitative reverse-transcription polymerase chain reaction (qRT-PCR). In addition, potential targets were predicted for differentially expressed miRNAs based on sequence complementation between miRNAs and their target genes. Gene ontology (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG) annotation revealed miRNAs that actively participate in various life processes and three pathways associated with protein synthesis including endoplasmic reticulum pathway, ribosome pathway, and ribosome biogenesis in eukaryotes, were significantly disrupted in MSG-Nd. This is the first comprehensive description of miRNAs in the silkworm MSG. Overall, the results provide useful information for future studies on miRNAs and suggest that the fibroin synthetic deficiency in the posterior silk gland impairs the sericin secretion process in MSG.
Highly Efficient Shoot Regeneration from Cotyledonary Nodes of Vegetable Soybean
Qian-Qian Liu,Gang Chen,Jun-Yi Gai,Yue-Lin Zhu,Li-Fei Yang,Guo-Ping Wei,Cong Wang 한국원예학회 2010 원예과학기술지 Vol.28 No.2
풋콩(Glycine max (L.) Merrill) 6개 품종의 자엽절 절편체로부터 thidiazuron(TDZ)과 NAA 농도를 달리한 배지에서 신초의 효율적인 재분화를 조사한 후, 가장 효과가 좋은 생장조절물질 조합으로 배지 종류, 접종방법 및 품종별 신초 재분화율을 조사하였다. 또한 절편체를 배지에 수직 또는 수평으로 치상하는 방법과 B5, 1/2 B5, MS, 1/2 MS, MSB(MS salts + B5 organics) 등의 5가지 기본 배지에 관하여 시험하였다. B5 배지에 1㎎?L<SUP>-1</SUP> TDZ, 0.05㎎?L<SUP>-1</SUP> NAA, 5㎎?L<SUP>-1</SUP> AgNO₃를 첨가한 처리에서 6개 품종의 신초 재분화율이 55.3-88.9%로 높았다. ‘L?ling No. 1’의 경우 수직으로 치상하는 것이 수평으로 치상하는 것보다 신초 재분화율이 37.5% 더 높았다. 따라서 풋콩은 TDZ를 처리한 B5 배지에 자엽절을 수직으로 치상할 때 신초 재분화율이 높았다. To establish a highly efficient system for shoot regeneration in vegetable soybean (Glycine max (L.) Merrill), explants were obtained from six genotypes and adventitious shoots were regenerated from cotyledonary nodes cultured on medium supplemented with different concentrations of N-phenyl-N′-1, 2, 3-thiadiazol-5-ylurea (thidiazuron TDZ) and 1-naphthylacetic acid (NAA). The most effective combination of plant growth regulators was selected first and then the effects of medium types, inoculation methods and genotypic differences on shoot regeneration were studied. The explants were inserted either vertically or horizontally into the medium and five basal media, including B5, ½ B5, MS, ½ MS, and MSB (MS salts and B5 organics), were tested. The shoot regeneration frequency of the six genotypes ranged from 53.5% to 88.9% and three of them reached 88.9%, 87.5% and 83.3%, respectively, on B5 medium supplemented with 1 ㎎?L<SUP>-1</SUP> TDZ, 0.05 ㎎?L<SUP>-1</SUP> NAA, and 5 ㎎?L<SUP>-1</SUP> AgNO₃. The shoot regeneration frequency of explants cultured on B5 medium was significantly higher than that of the other four basal media. The vertically inserted explants were found to yield a higher shoot regeneration frequency than that of horizontally inserted ones; the maximum difference of regenerating percentages between the two methods was 37.5%. In summary, TDZ was an efficient plant growth regulator for shoot induction. B5 medium and vertically inserted explants promoted shoot regeneration. We believe this highly efficient shoot regeneration system will provide foundation for the further transgenic studies in vegetable soybean.
Qian-Qian Wang,Li Cheng,Bi-Yu Wu,Hong-Yi Qiu,Ping Xu,Bo Wang,Xiu-Juan Yan,Sheng-Liang Chen 대한소화기 기능성질환∙운동학회 2023 Journal of Neurogastroenterology and Motility (JNM Vol.29 No.3
Background/AimsStigma related with antidepressants is prevalent in patients with functional dyspepsia. It affects medication compliance and efficacy. Herbal medicine acquired a deep-rooted cultural identity in relieving dyspeptic symptoms in Asians. The research was designed to compare the effectiveness of Zhizhu Kuanzhong capsules (ZZKZ) versus doxepin hydrochloride (doxepin) on alleviating stigma and medication nonadherence among patients with refractory FD (rFD). MethodsPatients with rFD from February 2021 to February 2022 were randomly allocated to receive either doxepin (n = 56) or ZZKZ (n = 57) combined with omeprazole for 4 weeks. Medication possession ratio (MPR), the disease- and medication-associated stigma were analyzed. The scales were utilized to assess dyspeptic symptoms (Leeds Dyspepsia Questionnaire) and psychological conditions (Generalized Anxiety Disorder Questionnaire and Patient Health Questionnaire). ResultsThe MPR values for ZZKZ were significantly higher than those for doxepin (P < 0.001). The stigma scores decreased in ZZKZ group while increased in doxepin group compared to baseline after treatment. The proportion of patients showing ZZKZ-associated stigma was significantly lower than doxepin-associated stigma (P < 0.001). The MPR values were negatively correlated with post-treatment stigma scores in both groups (P < 0.001). Dyspeptic symptoms and psychological condition were improved in both groups after treatment, with no significant difference on post-treatment Leeds Dyspepsia Questionnaire, Generalized Anxiety Disorder Questionnaire, or Patient Health Questionnaire scores between 2 groups. ConclusionZZKZ is superior to doxepin in alleviating stigma and medication non-adherence, with comparable efficacy in improving dyspeptic symptoms and psychological condition of patients with rFD.
Qian, Li-Yuan,Li, Ping,Li, Xiao-Rong,Chen, Dao-Jin,Zhu, Shai-Hong Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.8
Aims: To explore the relationship between various molecular makers and liver metastasis of colorectal cancer (CRC). Method: Using immunohistochemistry, protein expression of CEA, nm23, c-met, MMP2, COX-2, VEGF, EGFR, and CD44 was assessed in 80 CRC cases. The Chi-square test and logistic regression were performed to analyze the relationship between these indicators and CRC liver metastasis. Results: There were significant differences in expression of CEA, MMP2, CD44, VEGF and EGFR between the liver metastasis and non metastasis groups (P < 0.05); no significant differences were noted for nm23, c-met, and COX-2 expression. Logistic regression analysis showed that only CEA, VEGF, and EGFR entered into the regression equation, and had significant correlations with CRC liver metastasis (${\alpha}$ inclusion= 0.10, ${\alpha}$ elimination = 0.15, R2 = 0.718). Conclusions: Combination detection of CEA, VEGF, and EGFR may be an effective means to predict CRC liver metastasis. Nm23, c-met, MMP2, COX-2, and CD44, in contrast, are not suitable as prognostic markers.