Rapid Detection of Streptococcus mutans Using an Integrated Microfluidic System with Loop-Mediated Isothermal Amplification

Wang Jingfu,Wang Jingyi,Chang Xin,Shang Jin,Wang Yuehui,Ma Qin,Shen Liangliang 한국미생물·생명공학회 2023 Journal of microbiology and biotechnology Vol.33 No.8

Streptococcus mutans is the primary causative agent of caries, which is one of the most common human diseases. Thus, rapid and early detection of cariogenic bacteria is critical for its prevention. This study investigated the combination of loop-mediated isothermal amplification (LAMP) and microfluid technology to quantitatively detect S. mutans. A low-cost, rapid microfluidic chip using LAMP technology was developed to amplify and detect bacteria at 2.2–2.2 × 106 colony-forming units (CFU)/ml and its detection limits were compared to those of standard polymerase chain reaction. A visualization system was established to quantitatively determine the experimental results, and a functional relationship between the bacterial concentration and quantitative results was established. The detection limit of S. mutans using this microfluidic chip was 2.2 CFU/ml, which was lower than that of the standard approach. After quantification, the experimental results showed a good linear relationship with the concentration of S. mutans, thereby confirming the effectiveness and accuracy of the custom-made integrated LAMP microfluidic system for the detection of S. mutans. The microfluidic system described herein may represent a promising simple detection method for the specific and rapid testing of individuals at risk of caries.

Removal and fate of carbamazepine in the microbial fuel cell coupled constructed wetland system

Jingyi Xie,Haixiao Li,Shiyu Wang,Hao Chen,Wei Jiang,Lin Zhang,Lianjie Wang,Yufeng Wu,Lirong Li,Xueqiang Lu 대한환경공학회 2022 Environmental Engineering Research Vol.27 No.3

Carbamazepine (CBZ), which is difficult to remove in the wastewater treatment system and easily forms toxic transformation products during the treatment process, is one of the priority pollutants of pharmaceuticals and personal care products (PPCPs). Increasing attention has been paid to explore their treatment technology without side effects from the treatment products. This study aims to reveal the removal and transformation of CBZ in the microbial fuel cell coupled constructed wetland (CW-MFC) system. The CW-MFC system was operated continuously at room temperature for nearly 80 days. The results show that CW-MFC system can effectively remove CBZ with an average removal rate of 97%. Three transformation products were identified by liquid chromatography−high-resolution mass spectrometry: 2-(2-oxoquinazolin-1(2H)-yl) benzoic acid (TP267), methyl 2-(2-oxoquinazolin-1(2H)-yl) benzoate (TP281), 2-(2,4-dioxo-3,4-dihydroquinazolin-1(2H)-yl) benzoic acid (TP283). Except TP281 in the influent, the other transformation products were formed in the system, which indicated that TP267 and TP283 were the main transformation products of CBZ. The formation pathway of transformation products could be explained by reactions including oxidation, hydrolysis, bond rupture and intramolecular reaction. The results also indicate that the CW-MFC system might be a promising technology for PPCPs treatment.

음악학과 학부생에 대한 온라인 화상 수업의 학습 고착성에 미치는 영향 요인 연구

왕징지 ( Jingyi Wang ) 인문사회 21 2023 인문사회 21 Vol.14 No.1

연구 목적: 본 연구는 음악학과 학부생에 대한 온라인 화상 수업의 학습 점도에 미치는 영향 요인을 연구하고 이를 통해 수업 방식에 대한 약간의 의견을 제시하는 데 있다. 연구 방법: 본 연구는 인지적 가치 이론과 기대일치이론의 용적 가치, 내용적 가치, 사회적 가치, 만족도를 기반으로 음악학과 학부생에 대한 온라인 화상 수업의 학습 고착성에 미치는 영향 메커니즘 모델을 구축하였다. 연구 내용: 본 연구는 458명의 음악학과 학부생을 대상으로 구조적 방정식을 통해 실증 검증을 진행하였다. 결론 및 제언: 음악학과 학부생의 인지적 가치 중 실용적 가치는 학습 점도에 현저한 정적 영향을 미쳤다. 실용적 가치와 내용적 가치, 사회적 가치는 만족도에 현저한 정적 영향을 미쳤다. 내용적 가치와 사회적 가치는 학습 점성에 현저한 영향을 미치지 않았지만, 만족도는 학습 점도에 현저한 정적 영향을 미쳤다. This study aims to investigate the mechanism influencing the learning stickiness of undergraduate musicology students in online video-based instruction, to suggest improvements for this teaching method. This study Draws on perceived value theory and expectation confirmation theory, and this study constructs a model of the mechanism influencing the learning stickiness of undergraduate musicology students in online video-based instruction in terms of practical value, content value, social value, and satisfaction. This study uses 458 undergraduate musicology students in online video-based instruction as a sample to carry out the empirical test through the structural equation model. Practical value has a direct and significant positive influence on learning stickiness; practical value, content value, and social value significantly influence learning stickiness. However, content value and social value do not significantly and positively influence learning stickiness, but satisfaction significantly and positively influences learning stickiness.

Transient virus-induced gene silencing of MaBAM9b efficiently suppressed starch degradation during postharvest banana fruit ripening

Liu Mengting,Li Meng,Wang Yudi,Wang Jingyi,Miao Hongxia,Wang Zhuo,Xu Biyu,Li Xinguo,Jin Zhiqiang,Liu Juhua 한국식물생명공학회 2021 Plant biotechnology reports Vol.15 No.4

The genetic basis of metabolic pathways that operate during fruit ripening needs to be understood before the nutritional value of the banana can be improved. The banana is a typical starch conversion fruit, and β-amylase is a key enzyme that may play an important role in starch degradation during the ripening process. Musa acuminata β-amylase 9b (MaBAM9b) is closely related to starch degradation. However, its exact function in starch degradation has not been demonstrated in banana. Stable genetic transformation to identify gene function is a time- and energy-consuming process. Thus, an efficient and rapid method is needed for functional identification. Virus-induced gene silencing (VIGS) is a reverse-genetics method based on RNA-mediated antiviral plant defense that has been used to rapidly identify gene functions in plants. The aim of this study was to optimize a transient VIGS system and functionally elucidate MaBAM9b in postharvest banana fruit. Using 2- to 4-mm-thick fruit slices, vacuum infiltration of suspensions of Agrobacterium strains carrying TRV1 and TRV2-MaBAM9b, 0.5% iodine-potassium-iodide (I2-KI) staining for 150 s, and 1:3 TRV1:TRV2-MaBAM9b cultivation at 30 mmHg for 30 s achieved an optical density (OD) of 0.8 at 600 nm; after being incubated on Murashige and Skoog (MS) media for 5 days (d), starch degradation was efficiently suppressed during postharvest banana fruit ripening, as determined by I2-KI staining, total starch content, β-amylase activity, soluble sugar content, and endogenous MaBAM9b expression. The system described here is particularly useful for studying genes and networks involved in starch conversion in fruits, which alone would not produce a visual phenotype. This system will provide a platform for functional genomics and fruit quality improvement in banana.

A Novel Face Recognition Algorithm Based on Improved Retinex and Sparse Representation

Jingyi bo,Yubin Wang,Zhuo Lin 보안공학연구지원센터 2016 International Journal of Signal Processing, Image Vol.9 No.8

In recent years, face recognition technology has been widely used as a kind of important modern biological recognition technology. As one of the main factors that affect the recognition rate, the illumination variation has attracted the attention of many researchers. In order to improve the face recognition under illumination variation condition, a novel face recognition algorithm based on improved Retinex and sparse representation is proposed in this paper. Retinex algorithm can be used to solve the problem of face illumination variation in face recognition, but it is easy to produce ‘halo’ phenomenon. In order to improve the face recognition rate under the change of illumination condition. In this paper, firstly, in order to eliminate the interference of illumination on face recognition, we apply the Retinex that is improved by partial differential equations to face image processing. Then, sparse representation is used to extract face feature vector, and the voting method is used to realize the face recognition. Finally, the performance of the algorithm is tested by 3 standard face databases. The results show that the proposed algorithm can improve the face recognition rate under different illumination conditions, and has good robustness to illumination.

Investigation on the Storage Method of Blurred Art Image with Retrieval and Processing

Jingyi bo,Yubin Wang,Yu Xiang Li 보안공학연구지원센터 2016 International Journal of Database Theory and Appli Vol.9 No.7

For the Chinese ancient art, stone carving experiences the etching of the natural environment and become blurred. How to process and store their images becomes a quite important problem. In this paper, an image process and storage method have been proposed. In this method, the blurred image will be firstly pretreated. Then, relative images which have high similarity with the blurred image will be retrieved in the database. Thirdly, blurred images are processed with template matching method to improve the clarity. Finally, processed images and the original images are stored in the dataset with classification. A blurred art image is used as the example to verify the validity of the method. The blurred image contains a person and a door. The query method is proposed with higher speed and precision, which is due to the pretreatment and mixed subgraph method. The pretreatment can improve the precision of the query content, while the mixed subgraph method can provide the completeness of the query content. Both the mechanism improves the retrieval precision. The blurred images excavated in the soil are often with pitting and rust, which is corresponding to the noise in the image process. In the image process step, the image is fixed up when the blurry points are big enough. The verification confirms the validity of the method.

Impact response of a novel flat steel-concrete-corrugated steel panel

Jingyi Lu,Yonghui Wang,Ximei Zhai,Hongyuan Zhou 국제구조공학회 2022 Steel and Composite Structures, An International J Vol.42 No.2

A novel flat steel plate-concrete-corrugated steel plate (FS-C-CS) sandwich panel was proposed for resisting impact load. The failure mode, impact force and displacement response of the FS-C-CS panel under impact loading were studied via drop-weight impact tests. The combined global flexure and local indentation deformation mode of the FS-C-CS panel was observed, and three stages of impact process were identified. Moreover, the effects of corrugated plate height and steel plate thickness on the impact responses of the FS-C-CS panels were quantitatively analysed, and the impact resistant performance of the FS-C-CS panel was found to be generally improved on increasing corrugated plate height and thickness in terms of smaller deformation as well as larger impact force and post-peak mean force. The Finite Element (FE) model of the FS-C-CS panel under impact loading was established to predict its dynamic response and further reveal its failure mode and impact energy dissipation mechanism. The numerical results indicated that the concrete core and corrugated steel plate dissipated the majority of impact energy. In addition, employing end plates and high strength bolts as shear connectors could prevent the slip between steel plates and concrete core and assure the full composite action of the FS-C-CS panel.

Study on Wireless Power Transfer for Drilling Tools

Jingyi. Yang,Yuelong. Wang,Yi. Gao 제어로봇시스템학회 2018 제어로봇시스템학회 국제학술대회 논문집 Vol.2018 No.10

In practice, loosely coupled transformers play an important role on wireless power transfer system. However, specific sleeve structure, which is designed particularly suitable for the environment of drilling engineering, results in coupling coefficient dropping rapidly that affects efficiency and output voltage. In this paper, a wireless power transfer method for drilling tools is proposed. Firstly, the materials and parameters of mechanical structure are chosen through analyzing reluctance circuit of the system. Secondly, the formulas of efficiency and output voltage are given out by analyzing mutual inductance model in a series-series resonance topology with simplified equations. Finally, optimal frequency is determined since the efficiency and output voltage affected by frequency, which is validated with MATLAB simulation results.

Constructing Digital Library Information Platform Based On Cloud Computing

Yubin Wang,Jingyi Bo,Weili Xu 보안공학연구지원센터 2014 International Journal of Future Generation Communi Vol.7 No.3

Histone demethylase KDM4A plays an oncogenic role in nasopharyngeal carcinoma by promoting cell migration and invasion

Zhao Jingyi,Li Bingyan,Ren Yongxia,Liang Tiansong,Wang Juan,Zhai Suna,Zhang Xiqian,Zhou Pengcheng,Zhang Xiangxian,Pan Yuanyuan,Gao Fangfang,Zhang Sulan,Li Liming,Yang Yongqiang,Deng Xiaoyu,Li Xiaole,C 생화학분자생물학회 2021 Experimental and molecular medicine Vol.53 No.-

Compelling evidence has indicated the vital role of lysine-specific demethylase 4 A (KDM4A), hypoxia-inducible factor-1α (HIF1α) and the mechanistic target of rapamycin (mTOR) signaling pathway in nasopharyngeal carcinoma (NPC). Therefore, we aimed to investigate whether KDM4A affects NPC progression by regulating the HIF1α/DDIT4/mTOR signaling pathway. First, NPC and adjacent tissue samples were collected, and KDM4A protein expression was examined by immunohistochemistry. Then, the interactions among KDM4A, HIF1α and DDIT4 were assessed. Gain- and loss-of-function approaches were used to alter KDM4A, HIF1α and DDIT4 expression in NPC cells. The mechanism of KDM4A in NPC was evaluated both in vivo and in vitro via RT-qPCR, Western blot analysis, MTT assay, Transwell assay, flow cytometry and tumor formation experiments. KDM4A, HIF1α, and DDIT4 were highly expressed in NPC tissues and cells. Mechanistically, KDM4A inhibited the enrichment of histone H3 lysine 9 trimethylation (H3K9me3) in the HIF1α promoter region and thus inhibited the methylation of HIF1α to promote HIF1α expression, thus upregulating DDIT4 and activating the mTOR signaling pathway. Overexpression of KDM4A, HIF1α, or DDIT4 or activation of the mTOR signaling pathway promoted SUNE1 cell proliferation, migration, and invasion but inhibited apoptosis. KDM4A silencing blocked the mTOR signaling pathway by inhibiting the HIF1α/DDIT4 axis to inhibit the growth of SUNE1 cells in vivo. Collectively, KDM4A silencing could inhibit NPC progression by blocking the activation of the HIF1α/DDIT4/mTOR signaling pathway by increasing H3K9me3, highlighting a promising therapeutic target for NPC.