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RISS 인기검색어
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- 저자 : Wang Yudi (검색결과 5 건)
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Liu Mengting,Li Meng,Wang Yudi,Wang Jingyi,Miao Hongxia,Wang Zhuo,Xu Biyu,Li Xinguo,Jin Zhiqiang,Liu Juhua 한국식물생명공학회 2021 Plant biotechnology reports Vol.15 No.4
The genetic basis of metabolic pathways that operate during fruit ripening needs to be understood before the nutritional value of the banana can be improved. The banana is a typical starch conversion fruit, and β-amylase is a key enzyme that may play an important role in starch degradation during the ripening process. Musa acuminata β-amylase 9b (MaBAM9b) is closely related to starch degradation. However, its exact function in starch degradation has not been demonstrated in banana. Stable genetic transformation to identify gene function is a time- and energy-consuming process. Thus, an efficient and rapid method is needed for functional identification. Virus-induced gene silencing (VIGS) is a reverse-genetics method based on RNA-mediated antiviral plant defense that has been used to rapidly identify gene functions in plants. The aim of this study was to optimize a transient VIGS system and functionally elucidate MaBAM9b in postharvest banana fruit. Using 2- to 4-mm-thick fruit slices, vacuum infiltration of suspensions of Agrobacterium strains carrying TRV1 and TRV2-MaBAM9b, 0.5% iodine-potassium-iodide (I2-KI) staining for 150 s, and 1:3 TRV1:TRV2-MaBAM9b cultivation at 30 mmHg for 30 s achieved an optical density (OD) of 0.8 at 600 nm; after being incubated on Murashige and Skoog (MS) media for 5 days (d), starch degradation was efficiently suppressed during postharvest banana fruit ripening, as determined by I2-KI staining, total starch content, β-amylase activity, soluble sugar content, and endogenous MaBAM9b expression. The system described here is particularly useful for studying genes and networks involved in starch conversion in fruits, which alone would not produce a visual phenotype. This system will provide a platform for functional genomics and fruit quality improvement in banana.
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Hesperidin Protects Against High-Fat Diet-Induced Lipotoxicity in Rats by Inhibiting Pyroptosis
Ruixi Luo,Weiyi Tian,Yudie Hu,La Wang,Wenjia Wang,Ping Wang,Zunli Ke,Didong Lou 한국식품영양과학회 2024 Journal of medicinal food Vol.27 No.2
It is currently thought that excess fatty acid-induced lipotoxicity in hepatocytes is a critical initiator in thedevelopment of nonalcoholic fatty liver disease (NAFLD). Lipotoxicity can induce hepatocyte death; thus, reducing lipotoxicityis one of the most effective therapeutic methods to combat NAFLD. Abundant evidence has shown that hesperidin(HSP), a type of flavanone mainly found in citrus fruits, is able to ameliorate NAFLD, but the molecular mechanisms areunclear. We previously reported that pyroptosis contributed to NAFLD development and that inhibiting pyroptosis contributedto blunting the progression of NAFLD in rat models. Therefore, we questioned whether HSP could contribute to amelioratingNAFLD by modulating pyroptosis. In this study, a high-fat diet (HFD) induced dyslipidemia and hepatic lipotoxicity in rats,and HSP supplementation ameliorated dyslipidemia and insulin resistance. In addition, the HFD also caused pyroptosis in theliver and pancreas, while HSP supplementation ameliorated pyroptosis. In vitro, we found that HSP ameliorated palmitic acidinducedlipotoxicity and pyroptosis in HepG2 and INS-1E cells. In conclusion, we showed for the first time that HSP has aprotective effect against liver and pancreas damage in terms of pyroptosis and provides a novel mechanism for the protectiveeffects of HSP on NAFLD.
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Preparation of N-TiO2/RGO nanocomposites through sol-gel method
Yudi Ma,Shukun Wang,Wanlan Zheng,Xin Xue,Huie Liu,Shuang Chen,Yiwen Zhu 한국화학공학회 2021 Korean Journal of Chemical Engineering Vol.38 No.9
Nitrogen-doped TiO2 and reduced graphene oxide (RGO) nanocomposites (NTG) were prepared by solgel method followed by annealing treatment process under N2 atmosphere. The as-prepared NTG nanocomposite were characterized by transmission electron microscopy (TEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), Fourier-transform infrared spectroscopy (FT-IR) and ultraviolet-visible diffuse reflectance spectroscopy (DRS). The results indicate that the incorporation of nitrogen onto both RGO and TiO2 was accomplished simultaneously in the facile process. Nitrogen doping makes the light excitation range red shift and can enhance the electron-hole separation effectively. The photocatalytic activity of the as-prepared samples was evaluated through the degradation of methyl orange (MO) under visible light irradiation. The introduction of nitrogen increased the photodegradation activity, which can be indicated by the fitted apparent first-order kinetics rate constant k, increasing about four times from 0- NTG-450 to 15-NTG-450. The annealing treatment further increased the photodegradation activity about 1.5 times of 15NTG-450 for 15NTG-800.
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Xian Jing,Kaixuan Wang,Jieqiong Lin,Peng Liu,Yudi Kan,Xu Zheng,Jie Sun,Yingchun Li 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2019 NANO Vol.14 No.1
Slicing reference data and adaptive slicing algorithm are the key issues to find out appropriate layer thicknesses of three-dimensional (3D) microstructures with free-form surface in two photon polymerization (TPP). In the present work, an adaptive slicing method (ASM) was proposed to improve the fabricating efficiency of 3D microstructures with free-form surface in TPP microfabrication. In the proposed method, slicing reference data were obtained by intersecting a series of vertical planes with the triangular patch format along characteristic directions. Maximum acceptable chordal height played a role as judging criteria to obtain appropriate layer thicknesses. What's more, typical 3D microstructures with free-form surfaces were fabricated with uniform slicing method (USM) and the proposed ASM, respectively. The comparison results showed that the proposed method effectively improved fabricating efficiency, guaranteeing an acceptable surface quality at the same time. This work was expected to provide references for improving fabrication efficiency of complex 3D microstructures in TPP.
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