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- 저자 : Lianjie Wang (검색결과 4 건)
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Yunwu Yu,Junhai Wang,Yan Wang,Wenhao Pan,Changwei Liu,Peng Liu,Lianjie Liang,Changwei Xu,Yunxue Liu 한국공업화학회 2020 Journal of Industrial and Engineering Chemistry Vol.83 No.-
Phenolphthalein-based cardo poly(ether ether ketone) (PEEKWC)/polyethyleneimine (PEI) crosslinkingmembranes were prepared by solution casting followed by solution reaction at room temperature. Theeffects of reaction time and PEI content on the membrane structure and gas separation performanceswere investigated. Light transmittance measure and AFM evidenced the relationship between themicrophase separation in PEEKWC-PEI membranes and reaction time. The CO2 permeability increasedwith the PEI content, while the N2 and CH4 permeability remained nearly constant. This result indicatedthat only CO2 was transported by the solution–diffusion mechanism and also that PEI exhibited a positivecontribution as afixed carrier for CO2 facilitated transport. Meanwhile the PEEKWC-PEI crosslinkingmembranes showed higher selectivities than the pure PEEKWC membrane. The highest CO2/N2 and CO2/CH4 selectivities were about 131 and 122 increasing from 33 and 30, respectively. The crosslinkingstructure of membranes improved the CO2 permeability stability.
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DNMT1 mediates chemosensitivity by reducing methylation of miRNA-20a promoter in glioma cells
Daoyang Zhou,Yingfeng Wan,Dajiang Xie,Yirong Wang,Junhua Wei,Qingfeng Yan,Peng Lu,Lianjie Mo,Jixi Xie,Shuxu Yang,Xuchen Qi 생화학분자생물학회 2015 Experimental and molecular medicine Vol.47 No.-
Although methyltransferase has been recognized as a major element that governs the epigenetic regulation of the genome during temozolomide (TMZ) chemotherapy in glioblastoma multiforme (GBM) patients, its regulatory effect on glioblastoma chemoresistance has not been well defined. This study investigated whether DNA methyltransferase (DNMT) expression was associated with TMZ sensitivity in glioma cells and elucidated the underlying mechanism. DNMT expression was analyzed by western blotting. miR-20a promoter methylation was evaluated by methylation-specific PCR. Cell viability and apoptosis were assessed using the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) and TdT-mediated dUTP-biotin nick end labeling assays, respectively. The results showed that compared with parental U251 cells, DNMT1 expression was downregulated, miR-20a promoter methylation was attenuated and miR-20a levels were elevated in TMZ-resistant U251 cells. Methyltransferase inhibition by 5-aza-2′-deoxycytidine treatment reduced TMZ sensitivity in U251 cells. In U251/TM cells, DNMT1 expression was negatively correlated with miR-20a expression and positively correlated with TMZ sensitivity and leucine-rich repeats and immunoglobulin-like domains 1 expression; these effects were reversed by changes in miR-20a expression. DNMT1 overexpression induced an increase in U251/TM cell apoptosis that was inhibited by the miR-20a mimic, whereas DNMT1 silencing attenuated U251/TM cell apoptosis in a manner that was abrogated by miR-20a inhibitor treatment. Tumor growth of the U251/TM xenograft was inhibited by pcDNA-DNMT1 pretreatment and boosted by DNMT1-small hairpin RNA pretreatment. In summary, DNMT1 mediated chemosensitivity by reducing methylation of the microRNA-20a promoter in glioma cells.
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Removal and fate of carbamazepine in the microbial fuel cell coupled constructed wetland system
Jingyi Xie,Haixiao Li,Shiyu Wang,Hao Chen,Wei Jiang,Lin Zhang,Lianjie Wang,Yufeng Wu,Lirong Li,Xueqiang Lu 대한환경공학회 2022 Environmental Engineering Research Vol.27 No.3
Carbamazepine (CBZ), which is difficult to remove in the wastewater treatment system and easily forms toxic transformation products during the treatment process, is one of the priority pollutants of pharmaceuticals and personal care products (PPCPs). Increasing attention has been paid to explore their treatment technology without side effects from the treatment products. This study aims to reveal the removal and transformation of CBZ in the microbial fuel cell coupled constructed wetland (CW-MFC) system. The CW-MFC system was operated continuously at room temperature for nearly 80 days. The results show that CW-MFC system can effectively remove CBZ with an average removal rate of 97%. Three transformation products were identified by liquid chromatography−high-resolution mass spectrometry: 2-(2-oxoquinazolin-1(2H)-yl) benzoic acid (TP267), methyl 2-(2-oxoquinazolin-1(2H)-yl) benzoate (TP281), 2-(2,4-dioxo-3,4-dihydroquinazolin-1(2H)-yl) benzoic acid (TP283). Except TP281 in the influent, the other transformation products were formed in the system, which indicated that TP267 and TP283 were the main transformation products of CBZ. The formation pathway of transformation products could be explained by reactions including oxidation, hydrolysis, bond rupture and intramolecular reaction. The results also indicate that the CW-MFC system might be a promising technology for PPCPs treatment.
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Yugan He,Qi Yan,Xiaoyu Chang,Meiying Zhu,Weiwei Wang,Menglong Dong,Lianjie Song,Junjiao Yang,Yaodong Huang 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2018 NANO Vol.13 No.5
A TiO2 photocatalyst with peony-like microstructures and a large percentage of exposed {001} facets was synthesized using a facile solvethermal method. The peony-like TiO2 was obtained using HF as a capping agent, TiCl4 as the precursor and ethanol as the solvothermal agent. The parameters which influence the mophology and formation mechanism of the products including the HF concentration, the reaction time and temperature and the solvothermal solvent, were investigated. The samples were characterized using field emission scanning electron microscopy, transmission electron microscopy, X-ray diffraction and N2 adsorption and desorption analysis. As the reaction time or reaction temperature increased, the morphology TiO2 changed from hexagonally assembled microspheres to peony-like microflowers which were composed of stacks of ultrathin nanosheets. The other reaction parameters also play a crucial role in the formation of the TiO2 microstuctures. Photocatalytic experiments showed that the synthesized TiO2 out-performed Degussa P25 in the photodegradation of methelene blue under a very weak UV light irradiation (power: 8 W, light intensity: 0.4mW cm -1).
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