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- 저자 : Li Xinguo (검색결과 4 건)
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Ji Fusang,Tang Lu,Yang Zhen,Li Yuanyuan,Wang Wenchang,Xu Ya,Li Shuangmei,Li Xinguo 한국식물생명공학회 2021 Plant biotechnology reports Vol.15 No.3
Salt stress severely impacts plant growth and development. Banana, a salt-sensitive plant, has been studied for decades with respect to its physiological and biochemical performances under salt stress. To understand the salinity tolerance mechanisms of banana, the RNA-seq technique is applsied to analyze the transcriptomic changes on roots treated with salt (60 mM NaCl) for 12 h and 24 h in comparison with those cultured in normal condition. Totally, 1466 and 2089 diferentially expressed genes (DEGs), including 542 up- and 924 down-regulated-; 507 up- and 1582 down-regulated genes are identifed after salt treatment at 12 h and 24 h, respectively. Functional annotation analysis indicates that a large number of genes can be involved in several aspects of salt stress response including stress signal transduction, ROS scavenging, osmoregulation and transcription factors (TFs). This study is the frst report of a comprehensive transcriptome-based gene expression profle to analyze banana response to salt stress. The outcomes of this study could facilitate further dissecting the molecular mechanism underlying salt stress response and provide a valuable platform for further genetic improvement of salt tolerance in banana breeding programs.
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Hypersonic Aero-Heating Ground-Test Simulation Technique
Li, Ruiqu,Yao, Dapeng,Sha, Xinguo,Gong, Jian The Society for Aerospace System Engineering 2015 International Journal of Aerospace System Engineer Vol.2 No.2
It would encounter some complicated flow fields, such as transition, separation, reattachment and disturbances, in the hypersonic flight. Thus, it is difficult to theoretically analyze the hypersonic aerothermodynamics effects, so that the ground-test simulation is thought of as one of the most important methods to improve the understanding level of the hypersonic aerothermodynamics. However, the aero-heating tests could not simulate all aerodynamics and geometry parameters in the real flight due to the differences between the experimental environments supplied by the ground facilities and the flight, so that the feasible technique for the ground-test simulation of the hypersonic aerothermodynamics effects is required to be advanced. The key parameters that are especially required to simulate for aero-heating tests are analyzed and one detailed approach is suggested to perform the experimental investigation on the hypersonic aero-heating effects in the ground facilities in this paper, and the tests are performed in the FD-20 gun tunnel of CAAA (China Academy of Aerospace Aerodynamics) to give out the data which could be used to confirm the equation from the theoretical analysis.
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Liu Mengting,Li Meng,Wang Yudi,Wang Jingyi,Miao Hongxia,Wang Zhuo,Xu Biyu,Li Xinguo,Jin Zhiqiang,Liu Juhua 한국식물생명공학회 2021 Plant biotechnology reports Vol.15 No.4
The genetic basis of metabolic pathways that operate during fruit ripening needs to be understood before the nutritional value of the banana can be improved. The banana is a typical starch conversion fruit, and β-amylase is a key enzyme that may play an important role in starch degradation during the ripening process. Musa acuminata β-amylase 9b (MaBAM9b) is closely related to starch degradation. However, its exact function in starch degradation has not been demonstrated in banana. Stable genetic transformation to identify gene function is a time- and energy-consuming process. Thus, an efficient and rapid method is needed for functional identification. Virus-induced gene silencing (VIGS) is a reverse-genetics method based on RNA-mediated antiviral plant defense that has been used to rapidly identify gene functions in plants. The aim of this study was to optimize a transient VIGS system and functionally elucidate MaBAM9b in postharvest banana fruit. Using 2- to 4-mm-thick fruit slices, vacuum infiltration of suspensions of Agrobacterium strains carrying TRV1 and TRV2-MaBAM9b, 0.5% iodine-potassium-iodide (I2-KI) staining for 150 s, and 1:3 TRV1:TRV2-MaBAM9b cultivation at 30 mmHg for 30 s achieved an optical density (OD) of 0.8 at 600 nm; after being incubated on Murashige and Skoog (MS) media for 5 days (d), starch degradation was efficiently suppressed during postharvest banana fruit ripening, as determined by I2-KI staining, total starch content, β-amylase activity, soluble sugar content, and endogenous MaBAM9b expression. The system described here is particularly useful for studying genes and networks involved in starch conversion in fruits, which alone would not produce a visual phenotype. This system will provide a platform for functional genomics and fruit quality improvement in banana.
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