Slide Session : OS-IFD-07 ; Infectious Disease : In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus

( Myung Jin Lee ),( Kye Hyung Kim ),( Jong Youn Yi ),( Su Jin Choi ),( Chung Jong Kim ),( Nak Hyun Kim ),( Kyoung Ho Song ),( Pyoeng Gyun Choi ),( Ji Hwan Bang ),( Wan Beom Park ),( Eu Suk Kim ),( San 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus Myung Jin LEE1, Kye-Hyung KIM1, Jongyoun YI2, SuJin CHOI1, Chung-Jong KIM1, Nak- Hyun KIM1, Kyoung-Ho SONG1, Pyoeng Gyun CHOI1, Ji-Hwan BANG1, Wan Beom PARK1, Eu Suk KIM1, Sang-Won PARK1, Hong Bin KIM1, Nam Joong KIM1, Myoung- Don OH1 Seoul National University College of Medicine, Korea1, Pusan National University School of Medicine, Korea2 Background: Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease caused by a novel Bunyavirus, severe fever with thrombocytopenia syndrome virus (SFTSV). No effective antiviral therapy is proven yet, but clinical use of ribavirin (RBV) has been tried. We investigated the antiviral effect of RBV against SFTSV in vitro. Methods: To test for cytotoxicity of RBV, Vero cells were treated with different concentrations of RBV (3.90 to 500 μg/mL, two-fold dilution) and analyzed by cell viability MTS assay 48h post-infection. To determine antiviral activity of RBV against SFTSV, Vero cells were infected with SFTSV strain Gangwon/Korea/2012 at 100 TCID50 (50% tissue culture infective dose) per well in a 96-well plate, and RBV was added at the concentrations showing no or minimal cytotoxicity. Viral RNAs were extracted from the culture supernatants and quantifi ed using one-step real-time reverse transcription- PCR to amplify the partial large segment of SFTSV. Statistical analysis was done by one-way ANOVA with Tukey`s post hoc test. Results: Cytotoxicity due to RBV was not observed at RBV concentration =31.3 μg/ mL. Viral RNAs at 24h post-RBV treatment were reduced with increasing RBV concentrations (1-32 μg/mL), compared with those of mock-treated cells (P <0.01, Figure). Half maximal inhibitory concentration (IC50) of RBV was 3.69 μg/mL at 24h post-RBV treatment. Conclusions: Our study shows that RBV has antiviral effect against SFTSV in a dose-dependent manner. Further studies are required to evaluate the effi cacy of RBV in SFTS.

내시경적 점막절제술로 치험한 식도 과립상 세포종 1례

강혁주,김성욱,최석진,이중현,장재식,서영범,윤병구,박건욱,김성자,김용섭,강승완,이구,양창헌,이창우,김욱년,이광헌,서정일 동국대학교 의학연구소 2000 東國醫學 Vol.7 No.-

과립상 세포종은 Schwann 세포 기원으로 생각되며 인체에 비교적 드물게 발생한다. 과립상 세포종은 전신 어느 곳에서나 발견될 수 있으나 주로 혀, 구강, 피부 혹은 유방 등에서 호발하며 드물게 위장관에서 발견된다. 위장관에서는 식도에서 가장 호발하며 다음으로 위, 대장 순이다. 과립상 세포종은 대부분, 특히 위장관에서는 양성이며 소수의 악성 병변이 보고되었다. 이러한 이유와 함께 수술 전의 진단이 어렵기 때문에 과립상 세포종에 대한 근본적인 치료는 현재까지 외과적 절제술이다. 최근에 시도되는 치료방법들로는 내시경적 레이저 치료, 용종절제술, 내시경적 점막 절제술 등이 있다. 저자들은 상부 소화관 내시경검사를 시행하여 식도 과립상 세포종을 진단하고 내시경적 점막 절제술을 시행하여 합병증 없이 퇴원하여 현재 재발없이 경과 관찰중인 1례를 경험하였기에 보고하는 바이다. Granular cell tumors, which occur infrequently, are probably of Schwann cell origin. They can occur almost anywhere in the body but usually affect the tongue, oral cavity, skin, or breasts and are rarely found in the gastrointestinal tracts. The esophagus is the most frequent gastrointestinal site, followed by the stomach and the colon. Granular cell tumors are generally benign, especially in the gastrointestinal tract, some malignant lesions have been reported. For this reason, and also because preoperative diagnosis is difficult, the standard treatment for granular cell tumor has until now been surgical excision. In recent years, other therapeutic methods is endoscopic laser therapy (ELT), polypectiomy, endoscopic mucosal resection (EMR). We report a case of esophageal granular cell tumor which was diagnosed by an endoscopy and managed using an endoscopic mucosal resection without complication.

급성백혈병환자에서 분리된 Staphylococcus epidermidis의 분자 역학적 특성 및 위험요인

김경미,이동건,김상일,최정현,한성숙,신완식 대한병원감염관리학회 2003 의료관련감염관리 Vol.8 No.2

GRAM 작업 관리용 확장형 실행환경 정보 서비스 시스템 설계

김완규,김현주,정미숙,장경일,박규석 진주산업대학교 산업과학기술연구소 2005 산업과학기술연구소보 Vol.- No.12

Grid computing is an emerging technology that promise to unify resources and computing power in many organizations together. It couples a wide variety of geographically distributed computational resources, and presents them as a unified integrated high-end computer. The Globus Resource Allocation and Management(GRAM) service provides for the management and remote execution of jobs defined using a standard Resource Specification Language(RSL) requested by client in grid environment. However, Currently the GRAM has very limited functionality to support for applications that required special execution environment. Here, we provide a proposal for Execution Environment Information interacted with broker to support the GRAM's special execution environment.

음성신호의 실시간 끝점 검출에 관한 연구

김성석,이완주 용인대학교 자연과학연구소 2000 自然科學硏究所論文誌 Vol.5 No.1

In this paper, we present a speech end-point detection method for real time speech processing. The algorithm uses short time energy and zero crossing rate as a statistical feature of speech. Statistical thresholds of energy and zero crossing rate are periodically recalculated from the background noise if the speech start-point has not been detected. And if speech does not continue for pre-defined period of time, A/D conversion stops. In experiments, the real-time end-point detector on Pentium III PC shows the error rate of 2% in an office environment.

부산 인근 연안해 지역의 퇴적환경 연구

김완수,석동우 한양대학교 이학기술연구소 2002 이학기술연구지 Vol.5 No.-

부산 인근 연안해 지역의 퇴적환경을 알아보기 위하여 천부지층탐사와 퇴적물 입도분석을 실시하였다. 연구지역은 낙동강에서의 미세한 퇴적물의 유입이 상당한 지역으로 유입된 퇴적물은 조류와 대마해류에 의해 직접적인 영향을 받는다. 퇴적물의 분포 상태는 내해에서 니질(mud)퇴적체가 두껍게 발달하고 있으며, 외해쪽으로 수심이 증가하면서 퇴적층의 두께도 얇아진다. 이러한 퇴적체는 홀로세 기간동안 퇴적된 최적물이며, 음향기반 하부의 퇴적물질은 저해수면 시기의 잔류퇴적물로 사료된다. 또한 탄성파 반사 형태로 과거 낙동강 하구의 흔적과 가스 유기물질의 존재 가능성을 확인 할 수 잇었으며, 분석된 천부지층탐사 해석과 입석분석 결과에 의한 퇴적물 분포 양상은 서로 잘 일치하고 있다. In order to investigate the depositional environments in the coastal sea off Pusan area, shallow seismic profiling explorations and grain size analyses were carried out. The study area was supplied by a significant amount of fine grain sediments delivered by the Nakdong River, whose distribution was again influenced by tidal currents and the Tsushima current in the coastal sea. Thick mud deposits are found along the shallow near shore area and the thickness of the deposit decreases in the open sea direction as the depth of water increases. The mud deposit is believed to be of the Holocene Epoch and the acoustic basement is interpreted as the relict deposit formed during the Pleistocene low sea-level period. Based on the characteristic seismic reflections patterns, the paleo-Nakdong River channel and the possible presence of organic gas bubbles are recognized. The characteristics and distribution of the sediment bodr based on the interpretation of the shallow setsmic profiling and the results of gain size analyses are consistent with each other.

한반도 동남 해역에서 채취한 퇴적물 시료에 대한 자기특성 연구

김완수,석동우 한양대학교 이학기술연구소 2003 이학기술연구지 Vol.6 No.-

한반도 남부 해역에서 채취한 퇴적물 시추 코어에 대한 자기특성 측정을 통하여 각 시추코어간의 자기특성 변화 양상과 이를 근거로 한 퇴적환경의 차이를 알아보기 위하여 본 연구를 수행하였다. 진해만에서 2개(Site 7,8), 낙동감 남단에서 2개(Site 9,10), 그리고 거제도 동쪽 해역에서 1개(Site 1) 등 5개의 코어 시료를 채취하였다. 각 코어 시료에 대하여 특성 잔류자화 방향의 편각 과 복각, 대자율, 자화강도, 무자기이력잔류자화 획득실험, 등온잔류자화 획득실험을 실시하였으며, 이들 측정값의 상대값인 자기변수를 계산하였다. 각 코어 시료간의 수평적/수직적 자기특성의 비교를 통하여 코어간의 퇴적환경을 인지할 수 있다는 것을 보여주며, 특히 앞으로의 추가적인 확인과정을 통하면 자기특성에 영향을 미치는 환경을 지시하는 방법으로서의 가능성을 제시한다. In order to decipher the depositional environments in the southeast coastal sea of Korea, magnetic characterizations were carried out. Total of five sediment cores, two cores from the Jinhae Ba.y (Site 7 and 8), two from the southern part of Nakdong River and one core from the eastern sea off the Geoje Island, were collected using a piston corer. For each sediment cores, declination and inclination of characteristic remanent magnetization, intensity, magnetic susceptibility were measured and acquisition of anhysteretic and isothermal remanent magnetization experiments were performed. Based on the inter-core and intra-core comparisons of magnetic characteristics, it is shown that the magnetic characteristics can be used to interpret corresponding depositional environments. However, it is apparent that detailed measurements of sediment samples and careful correlation of data in the future study are necessary to establish the validity of the environmental magnetic study.

대형디젤기관에 미치는 습윤식 에어 필터의 영향

김미수,나완용,오용석 한국공작기계학회 2004 한국생산제조학회지 Vol.13 No.3

Diesel engine offers superior fuel consumption than gasoline engine of equivalent capacity. For this reason, diesel engines are widely used in heavy duty transport applications. Specially, it has been many years that exhaust gases from gasoline automobile rather than from diesel is the major object concerned by Korea and other countries, and it strongly required on the reduction techniques on harmful NOx, Soot, CO, HC. thus, this paper focused on the emission reduction and target for this paper is heavy-duty diesel engine equipped with power filter such as wet type air cleaner. In this paper, the performance, exhaust emissions(CO, THC, Nox, Soot) and noise of heavy-duty diesel engine were measured at maximum load condition and the range of 1,000~2,200pm. The smoke was measured at FAS(Free Accel Smoke) test mode.

이중공진 이중편파 마이크로스트립 안테나

김연정,윤완석,윤영중 延世大學校 電波通信共同硏究所 1999 電波通信論文誌 Vol.4 No.1

본 논문에서는 Ku-band 위성통신 송수신 겸용 안테나를 위해서 500 ㎒이상의 대역폭과 송·수신부가 서로 다른 선형편파를 가지는 이중급전 구조의 이중공진 안테나를 연구하였다. 이때 상대 급전선에 의한 임피던스의 변화를 최소화시켜 최적의 안테나를 설계하였으며, 배열 안테나로 확장시 이중급전 구조의 문제점인 공간상의 문제를 해결하기 위해서 마이크로스트립 선로와 via-hole 혼합급전 방식을 사용하였다. 제안된 안테나를 이차원의 2X2 배열 마이크로스트립 안테나로 설계 및 제작하였고, 방사패턴과 주파수특성을 측정하여 위성통신 송수신 겸용 안테나로 잘 동작할 수 있음을 확인하였다. In this paper, to transmit and receive the differently polarized signals simultaneously with a single antenna system, the dual-frequency microstrip array antenna with dual-polarization is proposed and designed. This antenna operates simultaneously at 14.25 ㎓ and 12.50 ㎓ with the bandwidth above 500㎒. To extend to two dimensional array, microstrip feed line and coaxial probe through via-hole are used.

그람음성 간균의 수술후 상기도 정착에 관한 연구

석민호,김완식,조양자 한양대학교 의과대학 1987 한양의대 학술지 Vol.7 No.1

Upper respiratory colonization and subsequent postoperative pneumonia caused by gram-negative bacilli are increased in patients undergoing surgical procedure. The present study was to investigate the effect of surgical procedure on postoperative respiratory colonization and subsequent infection caused by gram-negative bacilli. Among patients undergoing various major and minor operations at Hanyang university hospital during the months of November and December of 1985, 50 patients were chosen to evaluate the changes in upper respirtory colonization by gram-negative bacilli and receptivity of buccal epithelial cells to Pseudomonas aeruginosa, a common respiratory colonizer and pathogen. Upper respiratory colonization by gram-negative bacilli was significantly increased from preoperative 10% (5/50) to postoperative 29% (13/45). Klebisiella pneumoniae was the most frequent postopertative isolate (4 strains). Viability of buccal epithelial cells was decresed postoperaively (P<0.05). Receptivity of buccal epithelial cell to Pseudomonas aeruginosa was increased postoperatively (P<0.001) in mean numbers of attached bacteria per cell and in numbers of cells with minimal numbers of attached bacteria and these two parameters were extremely correlated (=0.999). Upper respiratory colonization rate of gram-negative bacilli and increase in receptivity of buccal epithelial cells to Pseudomonas aeruginosa was significantly greater in patients whose operation took more than 2 hours than in those whose operation lasted less than 2 hours (P<0.001). Data obtained in the present study suggest that changes in surface binding properties of upper respiratory epithelium may be an important factor in predisposing to postoperative respiratory infection by gram-negative bacilli.