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Mariadhas Valan Arasu,Vinod Kumar,Somasundar Ashok,Hyohak Song,Chelladurai Rathnasingh,Hee Jong Lee,Doyoung Seung,박성훈 한국생물공학회 2011 Biotechnology and Bioprocess Engineering Vol.16 No.6
Klebsiella pneumoniae is a suitable biocatalyst for the production of 1,3-propanediol (1,3-PDO) and 3-hydroxypropionic acid (3-HP) from glycerol. However, its commercial applications have been impeded due to its poor growth characteristics and the excessive production of lipopolysaccharide (LPS). To overcome these limitations, a new K. pneumoniae J2B (KpJ2B) strain was isolated from municipal waste anaerobic digester samples. The shake flask cultivation of this new strain under aerobic conditions showed a specific growth rate of 0.92/h, which is 1.13times higher than that achieved using the well studied K. pneumoniae DSMZ2026 (KpDSMZ). When the new strain was grown in a bioreactor under aerobic conditions using a fed-batch mode for 36 h, the biomass concentration (4.03g/L CDW) and productivity (0.15 g/L/h) were almost 2.2times higher than the corresponding values with KpDSMZ. Growth was accompanied by the production of 1,3-PDO (186 mM), lactic acid (235 mM), ethanol (170 mM), and acetic acid (92.2 mM) at significant levels, indicating the resistance of the strain to the inhibitory effects of these metabolites. A comparison of the SEM images and 2-keto-3-deoxyoctonate content (KpJ2B, 1.4 μg/g CDW; KpDSMZ,1.9 μg/g CDW) confirmed the lower LPS content in the KpJ2B strain. Furthermore, this new isolate exhibited higher sensitivity towards a range of antibiotics and better sedimentation properties than the KpDSMZ strain. This suggests that KpJ2B is an attractive strain for industrial applications.
Isolation of a Novel Pseudomonas Species SP2 Producing Vitamin B12 under Aerobic Condition
Mariadhas Valan Arasu,Ritam Sarkar,Balaji Sundara Sekar,Vinod Kumar,Chelladurai Rathnasingh,Jin-dal-rae Choi,Hyohak Song,Doyoung Seung,박성훈 한국생물공학회 2013 Biotechnology and Bioprocess Engineering Vol.18 No.1
Vitamin B12 is a complex biomolecule that acts as a cofactor for a variety of enzymes in microbial metabolism. Pseudomonas denitrificans is exclusively used as an industrial strain for the production of vitamin B12under aerobic conditions. However, only a few strains of Pseudomonas have been reported to possess the capability of producing this vitamin and they are strongly patentprotected. To improve the applicability of the vitamin B12-producing microorganisms, a new isolate was obtained from municipal waste samples and characterized for its biological properties. The new isolate, designated as SP2,was identified to be a Pseudomonas species based on the sequence homology of its 16S rDNA. Pseudomonas species SP2 had essential genes for vitamin B12 synthesis such as cobB and cobQ and produced a similar amount of vitamin B12 (10.6 ± 0.05 μg/mL) as P. denitrificans ATCC 13867 in 24 h flask culture. SP2 grew well under aerobic condition with the maximum specific growth rate (μmax) of 0.91 +0.03/h, but showed a poor growth under micro-aerobic conditions. SP2 was resistant to antibiotics like streptomycin,carbenicillin, ampicillin, cefpodoxime, colistin, nalidixic acid and sparfloxacin. The ability of SP2 to grow faster and produce vitamin B12 under aerobic conditions makes it a promising host for the production of some biochemicals requiring a coenzyme B12-dependent enzyme, such as glycerol dehydratase.
Importance of Scientific Writing
( Mariadhas Valan Arasu ) 한국환경농학회 2016 한국환경농학회 학술대회집 Vol.2016 No.-
Scientific manuscripts are one of the common tools in sharing the findings and inventions of students, research scholars, scientists and professors to the readers, investors and the funding agencies. Academic writing is broadly defined as any writing that can be backed up by part of research to explore a particular subject as part of an academic assignments. However, it is the art of exercise in communication and the ability of the writing skills would not be improved without tremendous practices. To the best of knowledge, before writing the manuscript, one should have clear blue print of the work in their mind, confidence and loyal data for further progress; which leads to clear flow in presenting their data. Skills such as subject competence, linguistic competence and organizational competence are essential in writing a good scientific manuscript. It is very important that the manuscripts should cover the important information’s of the finding which attract the readers more interestingly. In addition, the clearly presented information’s leads to scientific collaboration and get quick access to multi-disciplinary knowledge. Whereas, the researchers find difficulties in presenting their finding and publishing in the journal. In this presentation, I would like to cover important information considered for preparing the manuscripts.
클로렐라 첨가가 총체보리-크림슨 클로버 사일리지의 품질에 미치는 영향
최기춘,Mariadhas Valan Arasu,박형수,김원호,Soundarrajan Ilavenil 강원대학교 동물생명과학연구소(구 강원대학교 동물자원공동연구소) 2015 동물자원연구 Vol.26 No.1
The objective of the study was to investigate the effect of addition of chlorella (CA) on the change of nutritive values, organic acids and microbes on mixture of whole crop barley and crimson clover (WCB- CC) silage. This study was conducted at the experimental field of National Institute of Animal Science, Cheonan, Korea and consisted of three groups, such as control without CA, with 0.25% CA and with 0.5% CA. The contents of crude protein, total digestible nutrient, and in vitro dry matter digestibility of WCB-CC silage were increased by CA treatment (p<0.05). However, acid detergent fiber and neutral detergent fiber of WCB-CC silage were low compared to control (p<0.05). The pH of WCB-CC silage in CA treatment showed no significant difference, but the content of lactic acid and the number of lactic acid bacteria in CA treatment significantly increased as compared to control (p<0.05). These results suggest that the addition of CA could improve the quality of WCB-CC silage.
Ilavenil, Soundarrajan,Arasu, Mariadhas Valan,Vijayakumar, Mayakrishnan,Jung, Min-Woong,Park, Hyung Soo,Lim, Young Cheol,Choi, Ki Choon The Korean Society of Grassland and Forage Science 2014 한국초지조사료학회지 Vol.34 No.3
The present study was planned to analyze the nutritional quality, microbial counts and fermentative acids in Italian ryegrass (IRG) 80% and alfalfa 20% (IRG-HV) mediated silage inoculated with lactic acid bacteria (LAB) as a probiotic strain for 3 months. Crude protein (CP), acid detergent fiber (ADF), and neutral detergent fiber (NDF), total digestible nutrient (TDN) and In-vitro dry matter digestibility (IVDMD), lactic acid bacteria (LAB), yeast and fungi counts and fermentation metabolites such as lactic acid, acetic acid and butyric acids were analyzed. The result shows that the nutritional quality and metabolite profiles of silage were significantly improved with LAB. For microbial counts, LAB showed dominant followed by yeast as compared with control silage. The pH of the silage also reduced significantly when silage inoculated with LAB. The result confirmed that silage preparation using different crops with L. plantarum inoculation is most beneficial for the farmers.
Variation of Major Glucosinolates in Different Varieties and Lines of Rocket Salad
천진혁,Mariadhas Valan Arasu,임용표,김선주 한국원예학회 2013 Horticulture, Environment, and Biotechnology Vol.54 No.3
Glucosinolate (GSL) contents in five varieties and eleven lines of rocket salad (Eruca sativa L.) were quantified using HPLC-UV at 227 nm. Eleven GSLs including five aliphatic (glucoraphanin, glucothiobeinin, glucobrassicanapin,glucoerucin, and dimeric 4-mercaptobutyl GSL), two indolyl (glucobrassicin and 4-methoxyglucobrassicin),one aromatic (gluconasturtiin) and three unknown GSLs were identified based on our data base. Aliphatic GSLs were noted as the predominant group with an average 89% of the total content. The highest total GSL amounts were documented in ‘Herb-Fragrant Vegetable’ (31.12 μmol·g-1 dry weight (DW)), whereas the lowest was in line 28612(9.91). Relatively 73% of lines among the lines documented the content more than 8 µmol·g-1 DW of dimeric 4-mercaptobutyl GSL, particularly lines 28615 and 28619 noted more than > 17 µmol·g-1 DW. The sum of three major GSLs (glucoraphanin, glucoerucin, and dimeric 4-mercaptobutyl GSL) attributed > 8 µmol·g-1 DW in all rocket salads,especially ‘Herb-Fragrant Vegetable’ and line 28613 (87 and 93% respectively) in the total GSL accumulation,likewise line 28612 and 28620 attributed > 90%. Indolyl GSLs were ranged 0.27-1.09 µmol·g-1 DW, accounted less than 6% of the total GSLs in varieties and lines. These results provide valuable information regarding the potential beneficial GSL contents individually in different varieties and lines of rocket salads.
A. Samuel Raja,A. Valan Arasu 대한기계학회 2014 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.28 No.4
Two wheeled motor bikes are playing an important role in urban passenger transportation owing to ease of handling and affordablecost. Maximum amount of unburnt hydrocarbons (HC) and carbon monoxide (CO) are emitted during cold start of spark ignition engines. The current work presents the possible reduction of cold start HC emissions of 150 CC motorbike spark ignition (SI) engine with ethanol-gasoline blends and/or with intake air heating by glow plug. Anhydrous ethanol was blended with unleaded gasoline in the range of0% (E0) to 20% (E20) by volume to be used as fuel. The experimented parameters were intake air temperature, exhaust gas temperature,fuel consumption and exhaust gas emissions. Without intake air heating, E10 was found to be the optimum to reduce the cold start HCemissions by 23%. With intake air heating in the range of 40°C to 70°C, maximum HC emissions reduction was 23.8% for neat gasolineat 50°C and 33.6% for E10 blend at 60°C.