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Ilavenil Soundharrajan,Palaniselvam Kuppusamy,Hyun Jeong Lee,Ki Choon Choi 한국초지조사료학회 2019 한국초지조사료학회 학술대회논문집 Vol.2019 No.06
Lactic acid bacteria (LAB) were isolated and analysed its fermentation ability in triticale powder at different moisture levels. Furthermore, the antibacterial activity of fermented silage extract against cattle pathogens was also studied. The isolated strains were P. pentosaceus (TC48) and L. brevis (TC50) that confirmed based on biochemical and 16srRNA sequences methods. Extract from LAB fermented silage showed higher antibacterial activity (inhibition zone diameters: 18~24.2 mm) against E. coli P. aeruoginosa, S. aureus and E. Fecalis than the non-inoculated silage extract. TC48 and TC52 strains exhibited high tolerance to artificial gastric, duodenal and intestinal fluids. In summary, lactic acid bacteria mediated fermentation of triticale silage extract showed great antibacterial activity with significant probiotic characteristics might be an effective and safe way to provide new strategies for reducing the incidence of pathogenic bacteria associated diseases in animals.
Ilavenil, Soundharrajan,Kim, Da Hye,Srigopalram, Srisesharam,Kuppusamy, Palaniselvam,Valan Arasu, Mariadhas,Lee, Kyung Dong,Lee, Jung Chae,Song, Yeon Hee,Jeong, Young-Il,Choi, Ki Choon ELSEVIER SCIENCE B.V.; AMSTERDAM 2017 JOURNAL OF FUNCTIONAL FOODS Vol.37 No.-
<P><B>Abstract</B></P> <P>Ferulic acid (FA), a ubiquitous natural phenolic component found in many plants and fruits, has a wide range of biomedical applications. However, action mechanism of FA involved in lipid accumulation remains unclear. In this study, lipid accumulation and changes in expression levels of genes and proteins associated with adipocyte differentiation were investigated. Oil red O staining and glycerol accumulation assay revealed that FA decreased lipid accumulation in cells. FA downregulated expression levels of C/EBP-β, C/EBP-α, PPAR-γ, and SREBP-1, but upregulated expression levels of p38MAPK, p44/42 (Erk 1/2), and AMPK-α phosphorylation in 3T3-L1 cells. FA effects on high fat diet-induced (HFD) obese mice were also investigated. FA lowered HFD-induced body weight gain of obese mice without affecting regular food intake. FA reduced serum levels of total cholesterol and triglycerides in HFD obese mice. Similar to results of <I>in vitro</I> study, FA inhibited adipogenesis and lipid accumulation via downregulating PPAR-γ while upregulating p38MAPK, p44/42 (Erk 1/2), and AMPK-α phosphorylation in Swiss albino mice.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Ferulic acid (FA) extracted from Italian ryegrass and identified by HPLC. </LI> <LI> FA inhibits adipocyte differentiation via downregulation of key factors-<I>invitro</I>. </LI> <LI> FA reduced body weight gain in HFD induced obese mice-<I>in-Vivo</I>. </LI> <LI> FA activates p38MAPK, p44/42 (Erk 1/2) and AMPK-α protein expression. </LI> <LI> FA is a potent dietary source for preventing risk of obesity and their related disorders. </LI> </UL> </P>
Beneficial Effects of Lactic Acid Bacteria Inoculation on Oat Based Silage in South Korea
Ilavenil, Soundharrajan,Srigopalram, Srisesharam,Park, Hyung Soo,Kim, Won Ho,Lee, Kyung Dong,Choi, Ki Choon The Korean Society of Grassland and Forage Science 2015 한국초지조사료학회지 Vol.35 No.3
The objective of the study was to measure the beneficial effects of lactic acid bacteria (LAB) inoculation on the nutritive value of oat silage collected from thirteen regions in the Republic of Korea. The contents of crude protein, acid detergent fiber (ADF), neutral detergent fiber (NDF) and crude ash (CA) were slightly lower in LAB inoculated silage when compared with the control silage, whereas inoculation of LAB resulted in increased total digestible nutrient (TDN). Higher number of LAB, but lower count of yeast and fungi indicated the effectiveness of the LAB inoculation on oat silage fermentation. LAB inoculation resulted in low pH silage, which may prevent undesirable microbial growth. The LAB inoculation promoted lactic acid dominant fermentation with marginal levels of acetic acid and butyric acid in oat silage. These data suggest that the LAB inoculation may preserve oat silage at better quality for ruminant animal production.
Ilavenil, Soundarrajan,Arasu, Mariadhas Valan,Vijayakumar, Mayakrishnan,Jung, Min-Woong,Park, Hyung Soo,Lim, Young Cheol,Choi, Ki Choon The Korean Society of Grassland and Forage Science 2014 한국초지조사료학회지 Vol.34 No.3
The present study was planned to analyze the nutritional quality, microbial counts and fermentative acids in Italian ryegrass (IRG) 80% and alfalfa 20% (IRG-HV) mediated silage inoculated with lactic acid bacteria (LAB) as a probiotic strain for 3 months. Crude protein (CP), acid detergent fiber (ADF), and neutral detergent fiber (NDF), total digestible nutrient (TDN) and In-vitro dry matter digestibility (IVDMD), lactic acid bacteria (LAB), yeast and fungi counts and fermentation metabolites such as lactic acid, acetic acid and butyric acids were analyzed. The result shows that the nutritional quality and metabolite profiles of silage were significantly improved with LAB. For microbial counts, LAB showed dominant followed by yeast as compared with control silage. The pH of the silage also reduced significantly when silage inoculated with LAB. The result confirmed that silage preparation using different crops with L. plantarum inoculation is most beneficial for the farmers.
Trigonelline attenuates the adipocyte differentiation and lipid accumulation in 3T3-L1 cells
Ilavenil, S.,Arasu, M.V.,Lee, J.C.,Kim, D.H.,Roh, S.G.,Park, H.S.,Choi, G.J.,Mayakrishnan, V.,Choi, K.C. G. Fischer 2014 Phytomedicine Vol.21 No.5
Trigonelline is a natural alkaloid mainly found in Trigonella Foenum Graecum (fenugreek) Fabaceae and other edible plants with a variety of medicinal applications. Therefore, we investigated the molecular mechanism of trigonelline (TG) on the inhibition of adipocyte differentiation and lipid accumulation in 3T3-L1 cells. Trigonelline suppressed lipid droplet accumulation in a concentration (75 and 100μM) dependent manner. Treatment of adipocyte with of TG down regulates the peroxisome proliferator-activated receptor (PPARγ) and CCAAT element binding protein (C/EBP-α) mRNA expression, which leads to further down regulation of other gene such as adiponectin, adipogenin, leptin, resistin and adipocyte fatty acid binding protein (aP2) as compared with respective control cells on 5th and 10th day of differentiation. Further, addition of triognelline along with troglitazone to the adipocyte attenuated the troglitazone effects on PPARγ mediated differentiation and lipid accumulation in 3T3-L1 cells. Trigonelline might compete against troglitazone for its binding to the PPARγ. In addition, adipocyte treated with trigonelline and isoproterenol separately. Isoproterenol, a lipolytic agent which inhibits the fatty acid synthase and GLUT-4 transporter expression via cAMP mediated pathway, we found that similar magnitude response of fatty acid synthase and GLUT-4 transporter expression in trigonelline treated adipocyte. These results suggest that the trigonelline inhibits the adipogenesis by its influences on the expression PPARγ, which leads to subsequent down regulation of PPAR-γ mediated pathway during adipogenesis. Our findings provide key approach to the mechanism underlying the anti-adipogenic activity of trigonelline.