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Proteomic Analysis of Stage-II Breast Cancer from Formalin-Fixed Paraffin-Embedded Tissues
Abdullah Al-Dhabi, Naif,Srigopalram, Srisesharam,Ilavenil, Soundharrajan,Kim, Young Ock,Agastian, Paul,Baaru, Rajasekhar,Balamurugan, Kannan,Choi, Ki Choon,Valan Arasu, Mariadhas Hindawi Publishing Corporation 2016 BioMed research international Vol.2016 No.-
<P>Breast cancer is the most frequently occurring disease among women worldwide. The early stage of breast cancer identification is the key challenge in cancer control and prevention procedures. Although gene expression profiling helps to understand the molecular mechanism of diseases or disorder in the living system, gene expression pattern alone is not sufficient to predict the exact mechanisms. Current proteomics tools hold great application for analysis of cancerous conditions. Hence, the generation of differential protein expression profiles has been optimized for breast cancer and normal tissue samples in our organization. Normal and tumor tissues were collected from 20 people from a local hospital. Proteins from the diseased and normal tissues have been investigated by 2D gel electrophoresis and MALDI-TOF-MS. The peptide mass fingerprint data were fed into various public domains like Mascot, MS-Fit, and Pept-ident against Swiss-Prot protein database and the proteins of interest were identified. Some of the differentially expressed proteins identified were human annexin, glutathione S-transferase, vimentin, enolase-1, dihydrolipoamide dehydrogenase, glutamate dehydrogenase, Cyclin A1, hormone sensitive lipase, beta catenin, and so forth. Many types of proteins were identified as fundamental steps for developing molecular markers for diagnosis of human breast cancer as well as making a new proteomic database for future research. </P>
Antonisamy, P.,Duraipandiyan, V.,Aravinthan, A.,Al-Dhabi, N.A.,Ignacimuthu, S.,Choi, K.C.,Kim, J.H. North-Holland ; Elsevier Science Ltd 2015 european journal of pharmacology Vol.750 No.-
The current study was aimed to investigate the gastroprotective effects of friedelin isolated from the hexane extract of leaves of Azima tetracantha. Ethanol-induced gastric ulcer model was used to investigate the gastroprotective effects of friedelin. Antioxidant enzymes, lipid peroxidation, nitric oxide, gastric vascular permeability, pro and anti-inflammatory cytokines and apoptosis level have been investigated. Ethanol caused severe gastric damage and friedelin pretreatment protected against its deleterious role. Antioxidant enzyme activities, anti-inflammatory cytokines, prostaglandin E<SUB>2</SUB> (PGE<SUB>2</SUB>), constitutive nitric oxide synthase (cNOS) and mucus weight have been increased significantly. However, the vascular permeability, pro-inflammatory cytokines, inducible nitric oxide synthase (iNOS), caspase-3 and apoptosis level have significantly been decreased after friedelin ingestion. The present study has clearly demonstrated the anti-ulcer potential of friedelin, these findings suggested that friedelin could be a new useful natural gastroprotective tool against gastric ulcer.
Metabolic profiling of pale green and purple kohlrabi (Brassica oleracea var. gongylodes)
박창하,여현지,김남수,박예은,김선주,Mariadhas Valan Arasu,Naif Abdullah Al-Dhabi,박수윤,김재광,박상언 한국응용생명화학회 2017 Applied Biological Chemistry (Appl Biol Chem) Vol.60 No.3
Kohlrabi (Brassica oleracea var. gongylodes) is a dietary Brassica vegetable with noted health-beneficial properties associated with its numerous metabolites. The aim of this study was to elucidate phenotypic variation between the two cultivars through comprehensive analysis of the relationship of their primary and secondary metabolites. High-performance liquid chromatography (HPLC) and gas chromatography time-of-flight mass spectrometry (GC-TOFMS) are considered useful tools for profiling primary and secondary metabolites. A total of 45 metabolites, including organic acids, amino acids, sugars, and an amine, were identified in pale green and purple kohlrabies using GC-TOFMS-based metabolic profiling. The resulting data sets were analyzed by principal component analysis to determine the overall variation, and the purple and pale green vegetables were separated by the score plots generated. Additionally, HPLC analysis of anthocyanins in both cultivars revealed that green kohlrabies did not contain any anthocyanidins, while 11 anthocyanins were quantified in the purple ones. Cyanidin was the dominant anthocyanin found in the purple cultivar, with cyanidin-3-(feruloyl)-diglucoside-5-glucoside being the major one. This study suggests that GC-TOFMS and HPLC are suitable tools to determine metabolic connection among various metabolites and describe phenotypic variation between green and purple kohlrabies.
Metabolomics of differently colored Gladiolus cultivars
김연복,박수연,박창하,박우태,김선주,하선화,Mariadhas Valan Arasu,Naif Abdullah Al-Dhabi,김재광,박상언 한국응용생명화학회 2016 Applied Biological Chemistry (Appl Biol Chem) Vol.59 No.4
Gladiolus (Gladiolus grandiflora Hort.) plants are a perennial favorite, known for their beautiful flowers of many different colors. In this study, we determined metabolic differences between seven Gladiolus cultivars with differently colored flowers by monitoring anthocyanin, carotenoid, and hydrophilic metabolites. We detected nine anthocyanins in the seven cultivars. Only the ‘Violetta’ cultivar contained all nine anthocyanins and it exhibited the highest anthocyanin content, whereas no anthocyanins were detected in the ‘New Wave’ (white color) or ‘Limoncello’ (yellow color) cultivars. In addition, we detected seven carotenoids, the contents of which varied significantly among the cultivars depending on the flower color. ‘Limoncello’ exhibited the highest levels of carotenoids. Of the seven carotenoids, b-carotene and lutein accumulated in the most cultivars. In addition, we identified 43 metabolites using gas chromatography–timeof- flight mass spectrometry. The levels of organic acids and sugars in the ‘New Wave’ cultivar differed significantly from those in the ‘Violetta’ and ‘Limoncello’ cultivars with a P value <0.01. Thus, our results may help in understanding the metabolic differences between differently colored Gladiolus cultivars and should be useful in future databases.
Subramanian Mutheeswaran,Antony Mariappan,Kamaraj Ragavendran,Vedapuri Porchezhiyan,Poovan Elankani,Naif Abdullah Al-Dhabi,Mariadhas Valan Arasu,Savarimuthu Ignacimuthu 경희대학교 융합한의과학연구소 2023 Oriental Pharmacy and Experimental Medicine Vol.23 No.2
An ethnobotanical survey was conducted in Sathuragiri hills in the Virudhunagar district of the state of Tamil Nadu, India. A total of 139 species belonging to 124 genera under 52 families was recorded as medicinally useful; they were mainly used by the local people to treat various diseases. The families like Fabaceae (18 species), Euphorbiaceae (9 Species) and Apocynaceae (7 Species) contained many important medicinal plants. Some of the important illnesses treated by these species were dermatological ailments, gastrointestinal problems, joint pain, fever and poisonous bites. The data are quantified and important species are highlighted. Conservation of these medicinal plants and local knowledge is the need of the hour.
Park, Chang Ha,Zhao, Shicheng,Yeo, Hyeon Ji,Park, Ye Eun,Baskar, Thanislas Bastin,Arasu, Mariadhas Valan,Al-Dhabi, Naif Abdullah,Park, Sang Un NATURAL PRODUCT COMMUNICATIONS 2017 Natural product communications Vol.12 No.4
<P>Three Agrobacterium rhizogenes strains were tested for their ability to transform the plant Morus alba L. and to induce production of the secondary metabolites betulin and betulinic acid. All the tested strains of A. rhizogenes (R1601, LBA9402 and R1000) were able to induce hairy root formation in leaf tissue explants. Strain LBA9402 had the highest rate of infection (92.7% +/- 8.8%), whereas strain R1601 had the lowest rate (87.4% +/- 9.3%). The highest number of hairy roots per explant (5.6 +/- 0.5) and the greatest root length (2.4 +/- 0.2 mm) were obtained with strain LBA9402. We also evaluated dry weight (a measure of growth) and betulin and betulinic acid production in hairy roots and found that the highest growth (167.8 +/- 14.5 mg/flask) occurred after infection with strain LBA9402. Furthermore the highest production of betulin (5.4 +/- 0.4 mg/g dry weight) and betulinic acid (2.3 +/- 0.2 mg/g dry weight) was noted using strain LBA9402. Among three elicitors, yeast extract showed the highest induction of betulin production (8.7 +/- 0.4 mg/g) and silver nitrate induced the highest yield of betulinic acid (4.1 +/- 0.2 mg/g). Our study showed that A. rhizogenes strain LBA9402 was the most effective of the three tested strains for production of transformed root cultures and betulin and betulinic acid.</P>
Park, Chang Ha,Kim, Young Seon,Li, Xiaohua,Kim, Haeng Hoon,Arasu, Mariadhas Valan,Al-Dhabi, Naif Abdullah,Lee, Sook Young,Park, Sang Un NATURAL PRODUCT COMMUNICATIONS 2016 Natural product communications Vol.11 No.6
<P>Carbohydrate sources play important roles in energy and growth of plants. Therefore, in this study, we investigated the optimal carbohydrate source in hairy root cultures (HRCs) of Scutellaria baicalensis infected with Agrobacterium rhizogenes strain R1000. The hairy roots were cultured in half-strength B5 liquid medium supplemented with seven different carbohydrates sources (sucrose, fructose, glucose, galactose, sorbitol, mannitol and maltose), each at a concentration of 100 mM, in order to identify the best carbon sources for the production of major flavones, such as wogonin, baicalin and baicalein. Sucrose, galactose and fructose markedly influenced the production of major flavones and were therefore chosen for subsequent experiments. HRC growth and flavone accumulation were examined following culture with 30, 100 and 150 mM sucrose, galactose and fructose, respectively. From these data, 150 mM sucrose was found to be the optimal carbon source for the enhancement of baicalein production and growth of S. baicalensis HRCs. Fructose caused the greatest increase in baicalin accumulation. Additionally, galactose was the optimal carbon source for wogonin production. These results provide important insights into the optimal growth conditions, particularly the appropriate carbohydrate source, for S. baicalensis.</P>
Antonisamy, Paulrayer,Kannan, Ponnusamy,Aravinthan, Adithan,Duraipandiyan, Veeramuthu,Valan Arasu, Mariadhas,Ignacimuthu, Savarimuthu,Abdullah Al-Dhabi, Naif,Kim, Jong-Hoon Hindawi Publishing Corporation 2014 The Scientific World Journal Vol.2014 No.-
<P><I>Chromobacterium violaceum</I>, Gram-negative bacteria species found in tropical regions of the world, produces a distinct deep violet-colored pigment called violacein. In the present study, we investigated whether violacein can promote a gastroprotective effect and verified the possible mechanisms involved in this action. For this study, an indomethacin-induced gastric ulcer rat model was used. The roles of biomolecules such as MPO, PGE<SUB>2</SUB>, pro- and anti-inflammatory cytokines, growth factors, caspase-3, NO, K<SUP>+</SUP>ATP channels, and <I>α</I><SUB>2</SUB>-receptors were investigated. Violacein exhibited significant gastroprotective effect against indomethacin-induced lesions, while pretreatment with L-NAME and glibenclamide (but not with NEM or yohimbine) was able to reverse this action. Pretreatment with violacein also restored cNOS level to normal and led to attenuation of enhanced apoptosis and gastric microvascular permeability. Our results suggest that violacein provides a significant gastroprotective effect in an indomethacin-induced ulcer model through the maintenance of some vital protein molecules, and this effect appears to be mediated, at least in part, by endogenous prostaglandins, NOS, K<SUP>+</SUP>ATP channel opening, and inhibition of apoptosis and gastric microvascular permeability.</P>