http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Rathnasingh, Chelladurai,Raj, Subramanian Mohan,Jo, Ji-Eun,Park, Sunghoon Wiley Subscription Services, Inc., A Wiley Company 2009 Biotechnology and bioengineering Vol.104 No.4
<P>3-Hydroxypropionic acid (3-HP) is a commercially valuable chemical with the potential to be a key building block for deriving many industrially important chemicals. However, its biological production has not been well documented. Our previous study demonstrated the feasibility of producing 3-HP from glycerol using the recombinant Escherichia coli SH254 expressing glycerol dehydratase (DhaB) and aldehyde dehydrogenase (AldH), and reported that an “imbalance between the two enzymes” and the “instability of the first enzyme DhaB” were the major factors limiting 3-HP production. In this study, the efficiency of the recombinant strain(s) was improved by expressing DhaB and AldH in two compatible isopropyl-thio-β-galactoside (IPTG) inducible plasmids along with glycerol dehydratase reactivase (GDR). The expression levels of the two proteins were measured. It was found that the changes in protein expression were associated with their enzymatic activity and balance. While cloning an alternate aldehyde dehydrogenase (ALDH), α-ketoglutaric semialdehyde dehydrogenase (KGSADH), instead of AldH, the recombinant E. coli SH-BGK1 showed the highest level of 3-HP production (2.8 g/L) under shake-flask conditions. When an aerobic fed-batch process was carried out under bioreactor conditions at pH 7.0, the recombinant SH-BGK1 produced 38.7 g 3-HP/L with an average yield of 35%. This article reports the highest level of 3-HP production from glycerol thus far. Biotechnol. Bioeng. 2009; 104: 729–739 © 2009 Wiley Periodicals, Inc.</P>
2P-352 Isolation of Bacillus licheniformis from soil samples for the production of 2,3-butanediol
송찬우,박종명,( Chelladurai Rathnasingh ),송효학 한국공업화학회 2017 한국공업화학회 연구논문 초록집 Vol.2017 No.1
In this study, isolation of risk group 1 Bacillus spp. from soil and root samples was carried out to find safe and efficient 2,3-BDO producers. Several candidate strains were obtained via schematic isolation and screening process and 2,3-BDO producing performance was further validated by flask-level cultivation. Also, feasibility test on economic utilization of most popular carbon (glucose, xylose, fructose, sucrose) and organic nitrogen (yeast extract, CSL) sources was performed to develop industrial medium. Next, batch and fed-batch fermentations based on aeration rate and agitation speed control strategies, respectively, to optimize dissolved oxygen level for efficient production of 2,3-BDO were carried out. <sup>**</sup>This work was supported by the Industrial Strategic Technology Development Program (No. 10050407) funded by the Ministry of Trade, Industry & Energy (MOTIE, Korea).