Flavobacterium dongtanense sp. nov., isolated from the rhizosphere of a wetland reed.

Xiao, Yi-Ping,Hui, Wei,Lee, Jung-Sook,Lee, Keun Chul,Quan, Zhe-Xue Society for General Microbiology 2011 International journal of systematic and evolutiona Vol.61 No.2

<P>Two strains of Gram-reaction-negative, rod-shaped, non-spore-forming, non-motile, aerobic bacteria, designated LW30(T) and LW29, were isolated from the rhizosphere of a wetland reed in Dongtan, Chongming Island, China. The strains formed pale-yellow colonies on R2A plates. Growth occurred at 4-37 C (optimum 30 C), at pH 6-9 (optimum pH 7-8) and in the presence of 0-3?% (w/v) NaCl (optimum 0-1?%). Oxidase and catalase activities and flexirubin-type pigments were absent. MK-6 was the major respiratory quinone. The major fatty acids were iso-C(15?:?0), C(15?:?0), iso-C(15?:?1) G and iso-C(17?:?1)ω9c. Strains LW30(T) and LW29 could be differentiated from related species by several phenotypic characteristics. Phylogenetic analyses based on 16S rRNA gene sequences placed strains LW30(T) and LW29 in the genus Flavobacterium with high sequence similarity to Flavobacterium cheniae NJ-26(T) (94.0?%) and Flavobacterium indicium GPTSA 100-9(T) (93.9?%). Together with F. indicium GPTSA 100-9(T), strains LW30(T) and LW29 formed a distinct group in the phylogenetic tree. The DNA G+C content was 30 mol%. On the basis of the phylogenetic and phenotypic evidence, strains LW30(T) and LW29 represent a novel species of the genus Flavobacterium, for which the name Flavobacterium dongtanense sp. nov. is proposed. The type strain is LW30(T) (=KCTC 22671(T) =CCTCC AB 209201(T)).</P>

Magnetic Properties in Iron Film System Deposited on Liquid Surfaces

Jian-Ping Xie,Quan-Lin Ye,Xiao-Jun Xu,Gao-Xiang Ye 한국자기학회 2008 한국자기학회 학술연구발표회 논문개요집 Vol.- No.-

Temporal and spatial impact of Spartina alterniflora invasion on methanogens community in Chongming Island, China

Xue Ping Chen,Jing Sun,Yi Wang,Heng Yang Zhang,Chi Quan He,Xiao Yan Liu,Nai Shun Bu,Xi-En Long 한국미생물학회 2018 The journal of microbiology Vol.56 No.7

Methane production by methanogens in wetland is recognized as a significant contributor to global warming. Spartina alterniflora (S. alterniflora), which is an invasion plant in China’s wetland, was reported to have enormous effects on methane production. But studies on shifts in the methanogen community in response to S. alterniflora invasion at temporal and spatial scales in the initial invasion years are rare. Sediments derived from the invasive species S. alterniflora and the native species Phragmites australis (P. australis) in pairwise sites and an invasion chronosequence patch (4 years) were analyzed to investigate the abundance and community structure of methanogens using quantitative real-time PCR (qPCR) and Denaturing gradient gel electrophoresis (DGGE) cloning of the methyl-coenzyme M reductase A (mcrA) gene. For the pairwise sites, the abundance of methanogens in S. alterniflora soils was lower than that of P. australis soils. For the chronosequence patch, the abundance and diversity of methanogens was highest in the soil subjected to two years invasion, in which we detected some rare groups including Methanocellales and Methanococcales. These results indicated a priming effect at the initial invasion stages of S. alterniflora for microorganisms in the soil, which was also supported by the diverse root exudates. The shifts of methanogen communities after S. alterniflora invasion were due to changes in pH, salinity and sulfate. The results indicate that root exudates from S. alterniflora have a priming effect on methanogens in the initial years after invasion, and the predominate methylotrophic groups (Methanosarcinales) may adapt to the availability of diverse substrates and reflects the potential for high methane production after invasion by S. alterniflora.

Deployment Dynamics of Large-Scale Flexible Solar Arrays with Deployable Mast

Hai-Quan LI,Xiao-Feng LIU,Shao-Jing GUO,Guo-Ping CAI 한국항공우주학회 2017 International Journal of Aeronautical and Space Sc Vol.18 No.2

In this paper, deployment dynamics of large-scale flexible solar arrays with deployable mast is investigated. The adopted solar array system is introduced firstly, then kinematic description and kinematic constraint equations are deduced, and finally, dynamics equation of the system is established by the Jourdain velocity variation principle and a new method to deal with topology changes of the deployable mast is introduced. The dynamic behavior of the system is studied in detail. Simulation results indicate that the proposed model is effective to describe the deployment dynamics of the solar arrays and that the introduced method is applicable for topology changes.

Potential industrial application of Actinobacillus succinogenes NJ113 for pyruvic acid production by microaerobic fermentation

Ke-quan Chen,Zhen Wang,Wen Xiao,Alie Zhang,Hanxiao Ying,Ping-Kai Ouyang 한국화학공학회 2016 Korean Journal of Chemical Engineering Vol.33 No.10

Actinobacillus succinogenes NJ113 is capable of microaerobic fermentation, which offers the possibility of a novel type of pyruvic acid production. A dissolved oxygen environment with stirring at 300 rpm was a key factor in the fermentative production of a maximum concentration of pyruvic acid. Potassium carbonate (K2CO3) was found to have a role in promoting pyruvic acid production, influencing the concentration of pyruvic acid and production of the by-product succinic acid. The final titer of pyruvic acid production was 36.8±0.1 g L−1 with an overall yield of 0.639±0.056 g g−1 glucose and 3.12±0.03mmol g−1 dry cell weight h−1. Significance and impact of the study: This study is the first to illustrate the advantage of using Actinobacillus succinogenes NJ113 with no genetic modification under microaerobic conditions for the production of pyruvic acid.

Henriciella marina gen. nov., sp. nov., a Novel Member of the Family Hyphomonadaceae Isolated from the East Sea

Zhe-Xue Quan,Dan-Ning Zeng,Yi-Ping Xiao,노성운,Young-Do Nam,Ho-Won Chang,윤정훈,오희목,배진우 한국미생물학회 2009 The journal of microbiology Vol.47 No.2

A bacterial strain, designated Iso4T, was isolated from the East Sea of Korea and was subjected to a polyphasictaxonomy study including phenotypic and chemotaxonomic characteristics as well as 16S rRNAgene sequence analysis. Cells of the strain were Gram-negative, motile, non-budding, non-stalked, andstrictly aerobic. Strain Iso4T grew optimally at 20°C in the presence of 1~2% (w/v) NaCl and at pH 6.9~7.6. The major respiratory quinone was Q-10 and the major cellular fatty acids were C18:1 ω7c (53.5%),C17:1 ω5c (11.7%), C17:1 ω6c (8.1%), C16:0 (7.8%), C17:0 (4.8%), C15:0 (2.9%), and C16:1 ω5c (2.2%). The DNAG+C content of strain Iso4T was 56.2 mol%. Phylogenetic analysis based on 16S rRNA gene sequencesshowed that strain Iso4T formed a monophyletic clade in the family Hyphomonadaceae, supported by highbootstrap value and was most closely related to the genus Hyphomonas (92~94%), a member of marinebacteria in the family. The phenotypic, genotypic, and chemotaxonomic evidences also suggest strain Iso4Trepresents a novel genus and species in the family Hyphomonadaceae, for which the name Henriciella gen. nov., sp. nov. is proposed. The type strain is Iso4T (=KCTC 12513T =DSM 19595T =JCM 15116T).

Deployment Dynamics of Large-Scale Flexible Solar Arrays with Deployable Mast

LI, Hai-Quan,LIU, Xiao-Feng,GUO, Shao-Jing,CAI, Guo-Ping The Korean Society for Aeronautical and Space Scie 2017 International Journal of Aeronautical and Space Sc Vol.18 No.2

In this paper, deployment dynamics of large-scale flexible solar arrays with deployable mast is investigated. The adopted solar array system is introduced firstly, then kinematic description and kinematic constraint equations are deduced, and finally, dynamics equation of the system is established by the Jourdain velocity variation principle and a new method to deal with topology changes of the deployable mast is introduced. The dynamic behavior of the system is studied in detail. Simulation results indicate that the proposed model is effective to describe the deployment dynamics of the solar arrays and that the introduced method is applicable for topology changes.

Numerical Study of the Effect of Grout Material Properties on Ground Deformation during Shallow TBM Tunneling

Qi Zhang,Xiao-Ping Zhang,Hao-Jie Wang,Quan-Sheng Liu,Dan Xu,Shao-Hui Tang 대한토목학회 2022 KSCE JOURNAL OF CIVIL ENGINEERING Vol.26 No.8

The full-face shield tunnel boring machine (TBM) has been widely used to build tunnels in urban area. Ground deformations usually occur during tunnelling processes, which is closely related to engineering geological property. A large ground deformation may lead to large-scale surface settlement or ground collapse, especially for shallow buried tunnels. In general, synchronous grouting is adopted to reduce the ground deformation. The temporal and spatial distribution of the grout pressure and consolidation processes of grouting have a significant influence on the deformation. For a better understanding the relationship between the grout material type and the ground deformation, the present study models a complex numerical model in FLAC3D based on the Guangzhou Metro line 18. A numerical method is proposed to reproduce the tunnelling process and the consolidation process of grout material. The effect of grouting material on ground deformation is discussed, i.e., immediately solidified type, quick hardening type, and good mobility type. The ground deformation of the numerical simulation are compared to those monitored in the field. The results indicate that the immediately solidified type grout can reduce the ground settlement effectively and is mainly applicable to secondary grouting. The quick hardening type can make the ground upheaval, which is smaller than that induced by immediately solidified type. The good mobility type can reduce the ground settlement and well control the ground deformation. The good mobility type recommends using in the numerical study for simulating the synchronous grouting.

Functional Roles of Long Non-coding RNA in Human Breast Cancer

Ye, Ni,Wang, Bin,Quan, Zi-Fang,Cao, San-Jie,Wen, Xin-Tian,Huang, Yong,Huang, Xiao-Bo,Wu, Rui,Ma, Xiao-Ping,Yan, Qi-Gui Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.15

The discovery of long noncoding RNA (LncRNA) changes our view of transcriptional and posttranscriptional regulation of gene expression. With application of new research techniques such as high-throughput sequencing, the biological functions of LncRNAs are gradually becoming to be understood. Multiple studies have shown that LncRNAs serve as carcinogenic factors or tumor suppressors in breast cancer with abnormal expression, prompts the question of whether they have potential value in predicting the stages and survival rate of breast cancer patients, and also as therapeutic targets. Focusing on the latest research data, this review mainly summarizes the tumorigenic mechanisms of certain LncRNAs in breast cancer, in order to provide a theoretical basis for finding safer, more effective treatment of breast cancer at the LncRNA molecular level.

Meta-analysis of Circulating Tumor Cells as a Prognostic Marker in Lung Cancer

Ma, Xue-Lei,Xiao, Zhi-Lan,Liu, Lei,Liu, Xiao-Xiao,Nie, Wen,Li, Ping,Chen, Nian-Yong,Wei, Yu-Quan Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.4

Introduction: Recent studies have shown that circulating tumor cells (CTCs) play potential roles as diagnostic and prognostic biomarkers with various cancer types. The aim of this study was to comprehensively and quantitatively summarize the evidence for the use of CTCs to predict the survival outcome of lung cancer patients. Materials and Methods: Relevant literature was identified using Medline and EMBASE. Patients' clinical characteristics, overall survival (OS) and progression-free survival (PFS) together with CTC positive rates at different time points (before, during and after treatment) were extracted. A meta-analysis was performed to clarify the prognostic role of CTCs and the correlation between the CTC appearance and clinical characteristics. Results: A total of 12 articles containing survival outcomes and clinical characteristics and 15 articles containing only clinical characteristics were included for the global meta-analysis. The hazard ratio (HR) for OS predicted by pro-treatment CTCs was 2.61 [1.82, 3.74], while the HR for PFS was 2.37 [1.41, 3.99]. The HR for OS predicted by post-treatment CTCs was 4.19 [2.92, 6.00], while the HR for PFS was 4.97 [3.05, 8.11]. Subgroup analyses were conducted according to histological classification and detection method. Odds ratio (OR) showed the appearance of pro-treatment CTCs correlated with the lymph node status, distant metastasis, and TNM staging, while post-treatment CTCs correlated with TNM staging only. Conclusion: Detection of CTCs in the peripheral blood indicates a poor prognosis in patients with lung cancer.