Chitosan Oligomer as a Hopeful Adjuvant for H5n1 Influenza Vaccine

( Le Van Hiep ),( Mai Thi Thanh ),( Dang Thi Hong Van ),( Vo Thi Phuong Khanh ),( Nguyen Anh Dzung ) 한국키틴키토산학회 2008 한국키틴키토산학회지 Vol.13 No.1

Effect of Chitosan oligomer (dp = 8-16) and other adjuvant such as: Aluminum phosphate (AlPO4) and Freund on immune response of H5N1 influenza vaccine in Swiss mice has been investigated. The Swiss mice were twice vaccinated alternative 20 days with dose 0.1 ml/mouse. After 10, 20 and 30 days, the immune response of Swiss mice with H5N1 vaccine was detected. The result showed that after 20 days of the first vaccination, immune response of chitosan oligomer test and Freund was 100%, but Freund test reduced 80% after 30days of vaccination. Meanwhile, immune response of the control and AlPO4 test was very low, only 40%. The Effect of chitosan oligomer and the others on antibody titer has been also investigated. The result showed that the antibody titer of chitosan oligomer test was 60 HIU (Hemagglutination units), 70 HIUs after 10 and 20 days of the first vaccination, and increased up to 112 HIUs after 10 days of the second vaccination and the antibody titer of the chitosan oligomer test was higher than the others. Therefore, chitosan oligomer is a hopeful adjuvant for H5N1 influenza vaccine.

SCN1A Gene Mutation and Adaptive Functioning in 18 Vietnamese Children with Dravet Syndrome

Thi Thu Hang Do,Diem My Vu,Thi Thuy Kieu Huynh,Thi Khanh Van Le,손은화,Thieu Mai Thao Le,Huu Hao Ha,Chi Bao Bui 대한신경과학회 2017 Journal of Clinical Neurology Vol.13 No.1

Background and Purpose Dravet syndrome is a rare and severe type of epilepsy in infants. The heterogeneity in the overall intellectual disability that these patients suffer from has been attributed to differences in genetic background and epilepsy severity. Methods Eighteen Vietnamese children diagnosed with Dravet syndrome were included in this study. SCN1A variants were screened by direct sequencing and multiplex ligation-dependent probe amplification. Adaptive functioning was assessed in all patients using the Vietnamese version of the Vineland Adaptive Behavior Scales, and the results were analyzed relative to the SCN1A variants and epilepsy severity. Results We identified 13 pathogenic or likely pathogenic variants, including 6 that have not been reported previously. We found no correlations between the presence or type of SCN1A variants and the level of adaptive functioning impairment or severity of epilepsy. Only two of nine patients aged at least 5 years had an adaptive functioning score higher than 50. Both of these patients had a low frequency of convulsive seizures and no history of status epilepticus or prolonged seizures. The remaining seven had very low adaptive functioning scores (39 or less) despite the variability in the severity of their epilepsy confirming the involvement of factors other than the severity of epilepsy in determining the developmental outcome. Conclusions Our study expands the spectrum of known SCN1A variants and confirms the current understanding of the role of the genetic background and epilepsy severity in determining the developmental outcome of Dravet syndrome patients.

Exposure to Tobacco Advertising and Promotion among School Children Aged 13-15 in Vietnam - an Overview from GYTS 2014

Tran, Khanh Long,Phung, Xuan Son,Kim, Bao Giang,Phan, Thi Hai,Doan, Thi Thu Huyen,Luong, Ngoc Khue,Pham, Thi Quynh Nga,Nguyen, Tuan Lam,Hoang, Van Minh,Le, Thi Thanh Huong Asian Pacific Journal of Cancer Prevention 2016 Asian Pacific journal of cancer prevention Vol.17 No.no.sup1

Evidence shows that tobacco advertising and promotion activities may increase tobacco consumption and usage, especially in youth. Despite the regulation on prohibiting advertisement of any tobacco product, tobacco advertisement and promotion activities are still common in Vietnam. This article presents current exposure to tobacco advertising and promotion (TAP) among school children aged 13 to 15 years in Vietnam in 2014 and potential influencing factors. Data from the Global Youth Tobacco Survey 2014 in Vietnam covering 3,430 school aged children were used. Both descriptive and analytical statistics were carried out with Stata 13 statistical software. Binary logistic regression was applied to explain the exposure to TAP among youth and examine relationships with individual factors. A significance level of p<0.05 and sampling weights were used in all of the computations. In the past 30 days, 48.6% of the students experienced exposure to at least 1 type of tobacco advertising or promotion. Wearing or otherwise using products related to tobacco was the most exposure TAP type reported by students (22.3%). The internet (22.1), points of sales (19.2) and social events (11.5) were three places that students aged 13-15 frequently were exposed to TAP. Binary logistic results showed that gender (female vs male) (OR = 0.61, 95%CI: 0.52 - 0.71), susceptibility to smoking (OR = 2.12, 95%CI: 1.53 - 2.92), closest friends' smoked (OR = 1.43, 95%CI: 1.2 - 1.7) and parents smoking status (OR = 2.83, 95%CI: 1.6 - 5.01) were significantly associated with TAP exposure among school-aged children. The research findings should contribute to effective implementation of measures for preventing and controlling tobacco use among students aged 13-15 in Viet Nam.

Proposal for the development of a national open access database in Vietnam and comparison with other Asian countries’ national literature databases

Loc My Thi Nguyen,Tien-Trung Nguyen,Thanh Thi Nghiem,Hien Thu Thi Le,Thao Phuong Thi Trinh,Thuan Van Pham,Thanh Chi Nguyen,Linh Khanh Hoang,Trung Tran 한국과학학술지편집인협의회 2020 Science Editing Vol.7 No.1

In the context of the need to ensure appropriate signalling of the publication of high-quality, international-calibre publications in Vietnam, as well as new policies to improve the quality and effectiveness of scientific research in Vietnam, it is practical to investigate the possibility of developing a national open access database (NOAD). This study aims to answer the question of whether it is necessary to establish a NOAD in Vietnam. We used document analysis to evaluate issues related to NOADs. The results of this study show the complexity, lack of consistency, and difficulty in obtaining practical statistics and assessing research and scientific records in Vietnam today. Furthermore, the findings of this study imply that it is necessary to establish a NOAD of scientific research in Vietnam. The information in this report can be used to develop a NOAD for Vietnam in particular, and for any country that lacks one in general.

Metagenomic analysis of bacterial community structure and diversity of lignocellulolytic bacteria in Vietnamese native goat rumen

Do, Thi Huyen,Dao, Trong Khoa,Nguyen, Khanh Hoang Viet,Le, Ngoc Giang,Nguyen, Thi Mai Phuong,Le, Tung Lam,Phung, Thu Nguyet,Straalen, Nico M. van,Roelofs, Dick,Truong, Nam Hai Asian Australasian Association of Animal Productio 2018 Animal Bioscience Vol.31 No.5

Objective: In a previous study, analysis of Illumina sequenced metagenomic DNA data of bacteria in Vietnamese goats' rumen showed a high diversity of putative lignocellulolytic genes. In this study, taxonomy speculation of microbial community and lignocellulolytic bacteria population in the rumen was conducted to elucidate a role of bacterial structure for effective degradation of plant materials. Methods: The metagenomic data had been subjected into Basic Local Alignment Search Tool (BLASTX) algorithm and the National Center for Biotechnology Information non-redundant sequence database. Here the BLASTX hits were further processed by the Metagenome Analyzer program to statistically analyze the abundance of taxa. Results: Microbial community in the rumen is defined by dominance of Bacteroidetes compared to Firmicutes. The ratio of Firmicutes versus Bacteroidetes was 0.36:1. An abundance of Synergistetes was uniquely identified in the goat microbiome may be formed by host genotype. With regard to bacterial lignocellulose degraders, the ratio of lignocellulolytic genes affiliated with Firmicutes compared to the genes linked to Bacteroidetes was 0.11:1, in which the genes encoding putative hemicellulases, carbohydrate esterases, polysaccharide lyases originated from Bacteroidetes were 14 to 20 times higher than from Firmicutes. Firmicutes seem to possess more cellulose hydrolysis capacity showing a Firmicutes/Bacteroidetes ratio of 0.35:1. Analysis of lignocellulolytic potential degraders shows that four species belonged to Bacteroidetes phylum, while two species belonged to Firmicutes phylum harbouring at least 12 different catalytic domains for all lignocellulose pretreatment, cellulose, as well as hemicellulose saccharification. Conclusion: Based on these findings, we speculate that increasing the members of Bacteroidetes to keep a low ratio of Firmicutes versus Bacteroidetes in goat rumen has resulted most likely in an increased lignocellulose digestion.

Anaplasma marginale and A. platys Characterized from Dairy and Indigenous Cattle and Dogs in Northern Vietnam

Nguyen Thi Hong Chien,Thi Lan Nguyen,Khanh Linh Bui,Tho Van Nguyen,Thanh Hoa Le 대한기생충학ㆍ열대의학회 2019 The Korean Journal of Parasitology Vol.57 No.1

Anaplasma marginale and A. platys were detected and characterized (16S rDNA sequence analysis) from dairy and indigenous cattle, and the latter in domestic dogs in Vietnam. A phylogenetic tree was inferred from 26 representative strains/species of Anaplasma spp. including 10 new sequences from Vietnam. Seven of our Vietnamese sequences fell into the clade of A. marginale and 3 into A. platys, with strong nodal support of 99 and 90%, respectively. Low genetic distances (0.2-0.4%) within each species supported the identification. Anaplasma platys is able to infect humans. Our discovery of this species in cattle and domestic dogs raises considerable concern about zoonotic transmission in Viet- nam. Further systematic investigations are needed to gain data for Anaplasma spp. and members of Anaplasmataceae in animal hosts, vectors and humans across Vietnam.

Dynamic stability of cholesterol and desmosterol in human milk from four asian countries

김지은,My Tuyen Thi Nguyen,김용기,정지아,Dan Li,Mai Xuan Hong To,Huynh Khanh Trang Nguyen,Beenish Israr,Thanh Van Le,안현주,김재한 한국식품과학회 2022 Food Science and Biotechnology Vol.31 No.12

Cholesterol is one of the functional nutrients in human milk, which is indispensable for infant growth. In this study, the concentration of cholesterol and desmosterol in human milk from four Asian countries (n = 578), including Korea, China, Vietnam, and Pakistan, were investigated. The average cholesterol concentrations of Korea and China were similar ranging between 90.2–91.6 mg/L, but those from Vietnam and Pakistan were higher at 113.8 and 175.7 mg/L, respectively. The relative standard deviations were 31–36%, except for Pakistan (51%), showing a broad distribution of 48 to 612 mg/L. Desmosterol concentrations were similar, ranging between 11.2 and 12.8 mg/L except for Pakistan, which was lower than other countries at 9.4 mg/L. In addition, the cholesterol and desmosterol concentrations during the lactation periods were not significantly different in all four Asian countries. Mothers’ BMI did not significantly impact the cholesterol and desmosterol concentration in maternal milk within the same country.

Efficacy of electrochemically activated water solution in gingivitis treatment

Dinh‑Toi Chu,Van‑Huy Pham,Thien Chu‑Dinh,Truong Nhu Ngoc Vo,Duc Long Duong,Van Nhon Bui,Minh Son Tong,Thi Thu Phuong Nguyen,Quynh Anh Le,Khanh‑Hoang Nguyen 한국약제학회 2019 Journal of Pharmaceutical Investigation Vol.49 No.3

Mouthwash is one of the most commonly oral healthcare products for prevention and treatment of gum diseases. Dr. ECA is a disinfectant solution manufactured by a Vietnamese company and has been licensed for oral healthcare, but its effect on gingivitis is still unclear. To evaluate the effectiveness of this product in the treatment of gingivitis, we conducted a clinical study in 60 students diagnosed with gingivitis to compare the results of gingivitis treatment using Dr. ECA and a control mouthwash (a demonstrated anti-gingivitis product). The data showed that in the treated group, the rate of good Gingivitis Index (GI) before treatment was 46.67%, but after 2 and 4 weeks of using Dr. ECA that rate was increased to 96.67% and 100%, respectively. The percentage of patients with bleeding on probing was 63.33% but declined sharply after 2 weeks of treatment to 6.67%, and further reduced to 3.33% after 4 weeks of treatment. The treatment outcomes in were similar in the Dr. ECA treated group and control one with no statistically significant difference. Our results suggest that the electrolyte saline solution Dr. ECA is effective in treating gingivitis. Its effects are similar to other mouthwash products that had been approved and marketed.

Outpatient Satisfaction at Private General Hospitals in Ho Chi Minh City, Vietnam

GIAO, Ha Nam Khanh,THY, Nguyen Thi Anh,VUONG, Bui Nhat,KIET, Truong Van,LIEN, Le Thi Phuong Korea Distribution Science Association 2020 The Journal of Asian Finance, Economics and Busine Vol.7 No.7

The quality of hospital services remains a concern of both the manager and the patient. The study aims to identify factors affecting outpatient satisfaction at private general hospitals in Ho Chi Minh City, establishing a scale for measuring them. Some 450 outpatients who were treated in five top private hospitals in Ho Chi Minh city (HCMC) in 2019 - An Sinh General Hospital, Hoan My General Hospital, Columbia Asia International Hospital, FV Hospital, and Vu Anh International General Hospital - were interviewed directly in the last quarter of 2019 to obtain the information. The SERVPERF model, plus the cost, together with the SPSS software, have been used to process information by Cronbach's alpha analysis, Exploratory Factor analysis, and linear regression analysis. The results show that there are five factors influencing outpatient satisfaction at private general hospitals in HCMC, in which four factors affects positively in the order of decreasing importance: treatment outcome, doctors and nurses' professional capacity, facilities and environment of the hospital, hospital care, and the treatment time factor affects negatively. The results of the study provide private hospital in HCMC managers with a number of suggestions to increase the level of hospital service quality, so that increase outpatients satisfaction.

Citrus diaspidids in Viet Nam: New, and confirmation of previous, records based on morphological and molecular verification of taxa

Hang T. Dao,George A.C. Beattie,Gillian W. Watson,Van Lam Pham,Van Liem Nguyen,Duc Khanh Le,Thi Hoa Nguyen,Viet Nguyen,Paul Holford 한국응용곤충학회 2018 Journal of Asia-Pacific Entomology Vol.21 No.1

Armoured scales (Hemiptera: Diaspididae) belong to the largest scale insect family and are among the mostinvasive insects in the world. Accurate identification of armoured scales is essential for systematic and phylogeneticstudies; biogeography; trade and plant quarantine; and pest management, particularly biological control. Several species are serious pests of citrus. Records of past field surveys conducted in Viet Nam between 1967 and2010 indicated the presence of 28 species on citrus there. Discrepancies in these records, and the retention ofspecimens of only five species in collections, led us to undertake surveys throughout the citrus-growing regionsof the country in 2013 and 2014 to verify previous records and conserve voucher specimens. The presence of 21diaspidid species was confirmed based on morphological and molecular data. The species observed werecommon but rarely abundant. Populations in commercial orchards may have been influenced by use of pesticides,but most species were recorded also in gardens and orchards where pesticide use was uncommon. Naturalenemies were abundant, but were not thoroughly documented for all the diaspidids we observed. An identificationkey to the species collected is provided. Differences between our findings and previous records from VietNam, from Yunnan and Guangxi in China, and from Indochinese countries neighbouring Viet Nam, indicate theneed for extensive surveys to fully document the diaspidid fauna on citrus in the region.