SCN1A Gene Mutation and Adaptive Functioning in 18 Vietnamese Children with Dravet Syndrome

Thi Thu Hang Do,Diem My Vu,Thi Thuy Kieu Huynh,Thi Khanh Van Le,손은화,Thieu Mai Thao Le,Huu Hao Ha,Chi Bao Bui 대한신경과학회 2017 Journal of Clinical Neurology Vol.13 No.1

Background and Purpose Dravet syndrome is a rare and severe type of epilepsy in infants. The heterogeneity in the overall intellectual disability that these patients suffer from has been attributed to differences in genetic background and epilepsy severity. Methods Eighteen Vietnamese children diagnosed with Dravet syndrome were included in this study. SCN1A variants were screened by direct sequencing and multiplex ligation-dependent probe amplification. Adaptive functioning was assessed in all patients using the Vietnamese version of the Vineland Adaptive Behavior Scales, and the results were analyzed relative to the SCN1A variants and epilepsy severity. Results We identified 13 pathogenic or likely pathogenic variants, including 6 that have not been reported previously. We found no correlations between the presence or type of SCN1A variants and the level of adaptive functioning impairment or severity of epilepsy. Only two of nine patients aged at least 5 years had an adaptive functioning score higher than 50. Both of these patients had a low frequency of convulsive seizures and no history of status epilepticus or prolonged seizures. The remaining seven had very low adaptive functioning scores (39 or less) despite the variability in the severity of their epilepsy confirming the involvement of factors other than the severity of epilepsy in determining the developmental outcome. Conclusions Our study expands the spectrum of known SCN1A variants and confirms the current understanding of the role of the genetic background and epilepsy severity in determining the developmental outcome of Dravet syndrome patients.

Involvement of NF-κB in changes of IFN-γ-induced CIITA/MHC-II and iNOS expression by influenza virus in macrophages

Hang, Do Thi Thu,Song, Jae-Young,Kim, Min-Young,Park, Jin-Woo,Shin, Yeun-Kyung Elsevier 2011 Molecular immunology Vol.48 No.9

<P><B>Abstract</B></P><P>Type II interferon (IFN-γ) plays an important role in defense against viral infection. Although this cytokine is found during influenza virus infection, it seems to have no protective function against the virus, and the reasons for this are not clear. To determine how the influenza virus overcomes the antiviral effects of IFN-γ, we examined the effect of A/Puerto-Rico/8/34 (H1N1) (PR8) infection on the expression of various IFN-γ inducible genes involved in defense against virus infection. The results showed that PR8 selectively affects IFN-γ induced MHC-II and iNOS expression in both the murine macrophage-like cell line, Raw264.7, and in primary alveolar macrophages. Infection of IFN-γ treated macrophages with PR8 resulted in decreased expression of CIITA/MHC-II and increased production of iNOS/NO. These changes correlate with activation of NF-κB but not with JAK/STAT signaling. The data indicate one possible mechanism underlying the ineffectiveness of IFN-γ against influenza virus, and suggest that NF-κB may be a promising target for anti-influenza drugs.</P>

Numerical Analysis of Dielectrophoresis-Based Microfluidic Chip with a Facing-Electrode Design for Cell Separation

Nguyen Thu Hang,Nguyen Mai Chi,Nguyen Hoang Trung,Thi Y Van Tran,Vu Ngoc Trung,Thu Hang Bui,Duc Trinh Chu,Bui Tung Thanh,Jen Chun-Ping,Quang Loc Do 한국농업기계학회 2024 바이오시스템공학 Vol.49 No.1

Purpose Circulating tumor cell separation and analysis have played a critical role in cancer diagnosis, prognosis, and treatment. In this work, we aim to design and investigate a novel biochip that integrates dielectrophoresis, microfl uidic technology to separate circulating tumor cells from blood cells. To create a dielectrophoresis-induced non-uniform electric fi eld, a facing-electrode design was proposed and utilized, in which a slanted electrode array and a simple rectangular ground electrode are placed parallel on the top and bottom parts of the microfl uidic channel, respectively. This design can reduce the particle position dependence in the microchannel and the complexity of the microfabrication process. Methods The separation process, effi ciency, and optimization of the proposed device were numerically investigated using the fi nite element method. Parametric research was conducted to comprehensively examine the impact of various operating and design factors on the cell movement and trajectories in the microfl uidic device. Results The results indicated the potential of the proposed biochip to ensure cancer cell separation from blood cells with high effi ciency, high purity in a label-free, non-invasive, easy integration, and low-cost manner. Under the optimal conditions, the separation effi ciency reached 92%, 88%, and 96% for human colon cancer cells (HT-29), red blood cells, and white blood cells, respectively. Conclusions In this study, a novel DEP-based microfl uidic chip was proposed to separate HT-29 tumor cells from blood cells and numerically investigated to verify the performance of the biochip design. Our fi ndings could provide a foundation for further theoretical and practical investigations. The proposed system can separate cancer cells from red blood cells and white blood cells as well as off ers numerous advantages, such as compact size, low voltage, high effi ciency, non-invasiveness, and label-free nature. The tumor cell enrichment platform has the potential for application in cancer detection, analysis, and assessment.

The Impact of Vietnam’s New Rural Development Program on Rural Household Income: Cross-sectional Evidence Covering Communes That Hosted Korea’s Saemaul Undong Program

Manh Hung Do,비엣 흥 응웬(Viet Hung Nguyen),투하 응웬 티(Thu Ha Nguyen Thi),한 호앙 티(Hanh Hoang Thi),프엉 투 응웬 티(Phuong Thu Nguyen Thi) 영남대학교 박정희새마을연구원 2018 새마을학연구 Vol.3 No.2

베트남의 1986년도 정치경제 개혁, 즉 도어머이(Doi Moi)는 베트남의 경제를 개방적 사회주의경제로 전환시켰으며, 이를 계기로 베트남 경제는 획기적으로 근대화되었다. 그러나 이 과정에서 급속한 도시화는 도농 간 불균형을 가져왔으며, 이 도농 간 격차를 줄이기 위해 베트남 정부는 2006년에 새농촌개발(NRD) 프로그램을 도입하기에 이르렀다. 이 프로그램은 농촌 지역의 사회기반시설의 확충과 생활환경의 개선, 생산 활동의 종합적 발전, 소득증대, 농민들의 육체적ㆍ정신적 건강 증진 등을 목표로 하고 있었다. 이 논문의 주된 목적은 NRD 프로그램의 시행이 NRD의 궁극적 목적 중 하나인 농가소득 증대에 미치는 영향에 대한 과학적 증거를 제시하는 데 있다. 또한 이와 더불어 새마을운동의 시범사업을 실시한 지역과 그렇지 않은 지역 간에 소득 증대에서 차이가 있는가를 다중회귀분석을 통해 밝혀보고자 한다. 분석 결과는 NRD의 시행이 상당히 긍정적 결과를 가져왔으며 코민 수준에서 농가소득 증대와 생활환경의 개선에 중요한 영향을 미쳤다는 것을 보여주었다. 또한 도로 건설, 전기 도입, 주민 역량 강화, 제도의 발전 등이 농가소득 증대에 큰 영향을 미쳤음을 확인했다. 회귀분석의 결과는 또한 새마을 사업을 시행하고 있는 코뮌들이 그렇지 않은 코뮌에 비해 연소득이 1200만 동(미화530달러) 더 많다(또는 41.8% 더 높다)는 것을 보여주었다. The economic and political reform of Vietnam in 1986 (the Doi Moi) has transformed its economy positively and significantly into an open socialist-oriented market economy. This caused a dramatic change of Vietnam’s economy towards modernization. However, the urbanization process created an unbalanced development between the urban and rural regions. To solve the gaps between these regions, the government of Vietnam firstly introduced the New Rural Development (NRD) program in 2006 to encourage and mobilize a revolutionary and campaign to improve local infrastructure, living condition, comprehensive development of related-production activities in rural regions, as well as increase income, and improve physical and mental health of people living in rural communities. One of the main purposes of this research paper is aimed at providing a scientific evidence for justifying whether the NRD implementation could have an impact on rural households’ income, one of the ultimate goals of the NRD, and examining the difference of incomes between Saemaul Undong (SU)-piloted provinces with the other provinces by employing a multiple regression model. The analysis results show that the NRD implementation has shown positive results and significant impacts on increasing rural households’ income and improving living conditions at the communal levels. Criteria such as road development, access to electricity, capacity building, and institutional development have significantly influenced on the households’ income. The regression model also indicates that the communes currently implementing SU pilots have a better annual income of 12 million VND (~US$ 530) or 41.8% higher than the other communes.

Cellobiose dehydrogenase from the agaricomycete Coprinellus aureogranulatus and its application for the synergistic conversion of rice straw

Nghi Do Huu,Kellner Harald,Büttner Enrico,Huong Le Mai,Duy Le Xuan,Giap Vu Dinh,Quynh Dang Thu,Hang Tran Thi Nhu,Verberckmoes An,Diels Ludo,Liers Christiane,Hofrichter Martin 한국응용생명화학회 2021 Applied Biological Chemistry (Appl Biol Chem) Vol.64 No.5

From the biotechnological viewpoint, the enzymatic disintegration of plant lignocellulosic biomass is a promising goal since it would deliver fermentable sugars for the chemical sector. Cellobiose dehydrogenase (CDH) is a vital component of the extracellular lignocellulose-degrading enzyme system of fungi and has a great potential to improve catalyst efficiency for biomass processing. In the present study, a CDH from a newly isolated strain of the agaricomycete Coprinellus aureogranulatus (CauCDH) was successfully purified with a specific activity of 28.9 U mg− 1. This pure enzyme (MW = 109 kDa, pI = 5.4) displayed the high oxidative activity towards β-1–4-linked oligosaccharides. Not least, CauCDH was used for the enzymatic degradation of rice straw without chemical pretreatment. As main metabolites, glucose (up to 165.18 ± 3.19 mg g− 1), xylose (64.21 ± 1.22 mg g− 1), and gluconic acid (5.17 ± 0.13 mg g− 1) could be identified during the synergistic conversion of this raw material with the fungal hydrolases (e.g., esterase, cellulase, and xylanase) and further optimization by using an RSM statistical approach.

The Impact of Corporate Social Responsibility on Brand Value and Financial Performance: Evidence from Bancassurance Service Providers in Vietnam

NGUYEN, Xuan Hung,DANG, Thuy Quynh,DINH, Thi Thao Quyen,DO, Phuong Thanh,PHAM, Thu Uyen,MAI, Duc Duong Korea Distribution Science Association 2022 The Journal of Asian Finance, Economics and Busine Vol.9 No.6

The objective of this article is to assess the impact of corporate social responsibility (CSR) implementation on Corporate Brand Value (CBV) and Corporate Financial Performance (CFP). At the same time, the article examines the mediated role of CBV on the relationship between CSR implementation and CFP. In-depth interviews and observation techniques were used in this article on 4 experts to collect qualitative information. Quantitative analysis was based on primary data obtained from 454 employees working at enterprises providing Bancassurance services. The article uses various models of Cronbach's Alpha coefficient, Exploratory Factor Analysis (EFA), Confirmatory Factor Analysis (CFA), and Structural Equation Analysis (SEM) using SPSS 22.0 and AMOS 20.0 software. In addition, a 5-point Likert scale is used to measure observed variables. Research results show that CBV plays an intermediate role in the relationship between CSR implementation and CFP. At the same time, the implementation of CSR toward communities, customers, and workers directly impacts CBV and CFP. The article draws a new conclusion; there is no relationship found between the implementation of CSR and CFP. The result proposes implications for the state and Bancassurance service providers to develop and implement CSR-related policies and activities to enhance their brand value and financial performance.

Screening of Vietnamese Medicinal Plants for Cytotoxic Activity

Nguyen Bich Thu,Trinh Nam Trung,Do Thi Ha,Nguyen Minh Khoi,Tran Viet Hung,Tran Thi Hien,Yim Namhui,배기환 한국생약학회 2010 Natural Product Sciences Vol.16 No.1

Thirty-two methanol extracts of thirty-one Vietnamese medicinal plants were evaluated for the cytotoxic activity against five human cancer cell lines, including A549, MCF-7, HT 1080, Huh-7, and HepG2. Of these, the nine extracts of Acanthopanax trifoliatus (4), Acanthopanax gracilistylus (5), Siegesbeckia orientalis (10), Betula alnoides (11), Passiflora edulis (18), Zanthoxylum simulans (leaf, 23), Adenosma caeruleum (26), Solanum verbascifolium (29), and Alpinia malaccensis (31), exhibited high potent cytotoxic activity showing a certain degree of selectivity against the different cell types, with IC50 values ranging from 2.1 to 3.8 mg/mL.

A Study on the Poverty of Mountain People Depending on Forests

NGUYEN, Phuong Thi Minh,NGUYEN, Song Van,DO, Duc Tai,NGUYEN, Quynh Thi Thuy,DINH, Thanh Trung,NGUYEN, Hang Phan Thu Korea Distribution Science Association 2020 The Journal of Asian Finance, Economics and Busine Vol.7 No.7

Livelihood capitals have a clear influence on livelihood development. As for the livelihood results, it has been pointed out in the analysis of the poor households that the ability of people to escape poverty depends especially on the access to livelihood capitals. This study aims to analyze the impacts of livelihood capital on poverty among mountain people who depend on forests through human capital, social capital, natural capital, physical capital and financial capital. This research employs the model of binary regression function. Independent variables x1, x2, …, xn are targets of livelihood strategy, vulnerability context, and livelihood capitals. These variables were selected to be included in the original model with dependent variable Y as poor and non-poor households. This study surveys households living in upland areas, near forests, and households of ethnic minorities. The results show that,out of the poor household rate, nearly 4% are newly-poor households or those falling back into poverty. Therefore, the government needs to pay more attention to this disadvantaged group and implements policies such as education and training policies, credit support policies, policies to support forest development, and payment for forest environmental services in the context of emerging countries like Vietnam.

BMB Reports : Crotamine stimulates phagocytic activity by inducing nitric oxide and TNF-α via p38 and NF?-B signaling in RAW 264.7 macrophages

( Kyung Jin Lee ),( Yun Kyu Kim ),( Martin Krupa ),( Anh Ngoc Nguyen ),( Bich Hang Do ),( Boram Chung ),( Thi Thu Trang Vu ),( Song Cheol Kim ),( Han Choe ) 생화학분자생물학회(구 한국생화학분자생물학회) 2016 BMB Reports Vol.49 No.3

Crotamine is a peptide toxin found in the venom of the rattlesnake Crotalus durissus terrificus and has antiproliferative, antimicrobial, and antifungal activities. Herein, we show that crotamine dose-dependently induced macrophage phagocytic and cytostatic activity by the induction of nitric oxide (NO) and tumor necrosis factor-alpha (TNF-α). Moreover, the crotamineinduced expression of iNOS and TNF-α is mediated through the phosphorylation of p38 and the NF-κB signaling cascade in macrophages. Notably, pretreatment with SB203580 (a p38- specific inhibitor) or BAY 11-7082 (an NF-κB inhibitor) inhibited crotamine-induced NO production and macrophage phagocytic and cytotoxic activity. Our results show for the first time that crotamine stimulates macrophage phagocytic and cytostatic activity by induction of NO and TNF-α via the p38 and NF-κB signaling pathways and suggest that crotamine may be a useful therapeutic agent for the treatment of inflammatory disease. [BMB Reports 2016; 49(3): 185-190]

Soluble expression and purification of secretory proteins in active form in the cytoplasm of E. coli

Han CHOE,Han-Bong RYU,Bon-Kyung KOO,Ji Young BAE,Seon Ha CHONG,Taek Hyun CHUNG,Jung A SONG,Seung-Mi JEONG,Nguyen Minh TAN,Vu Thi Thu TRANG,Hoang Thi PHUONG,Do Bich HANG 한국생물공학회 2011 한국생물공학회 학술대회 Vol.2011 No.10