Protein microarray를 이용한 Apin-단백질의 상호작용에 관한 연구

박주철,박선화,김흥중,박종태,윤성호,김지웅,이태연,손호현 대한치과보존학회 2007 Restorative Dentistry & Endodontics Vol.32 No.5

비소세포폐암에서 Microsatellite Instability

전효성,김정란,손지웅,박선하,박태인,김창호,김인산,정태훈,박재용 경북대학교 병원 2001 경북대학교병원의학연구소논문집 Vol.5 No.1

연구베경:MMR 유전자의 불활성화에 의해 야기되는 뮤전적 불안정성은 발암기전의 한 부류로 인정되고 있다.저자들은 비소세포폐암의 발암과정에서의 MSI의 역할을 규명하기 위해 비소세포폐암에서 MSI의 빈도 및 MSI 유무에 따른 임상상의 차이를 조사하였다. 대상 및 방법:근치적 절제술을 받은 비소세포폐암 20예를 대상으로 하였다.동결된 폐암조직과 환자의 림프구에서 DNA를 추출한 후 3P와 9p의 15개의 marker들을 대상으로 PCR을 시행하고 7% polyacrylamide gel에서 전기영동한 후 silver 염색을 시행하였다. 암조직과 림프구 DNA의 PCR product의 band를 비교하여 MSI와 LOH를 판정하였다. 결과:1)대상환자들은 남자 19예,여자 1예였으며 모두 흡연자였고 평균 흠연력은 47 갑년이었다. 폐암의 조직형은 편평상피암 15예, 선암 4예,대세포암 1예였고, 술 후 병리학적 병기는 Ⅰ기 6예,Ⅱ기 5예, ⅢA기 7예,ⅢB기 2예였다. 2)20예 가운데 8예(40%)에서 MSI가 관찰되었으며 3예는 한 개의 marker에서,5예는 2개 이상의 marker에서 MSI가 관찰되었다. 3)LOH는 10예(50%)에서 있었으며,LOH유무에 따른 병가 및 흡연력의 차이가 없었다. 4)분석한 marker의 10% 이상에서 MSI가 관찰된 MSI-L종양은 5예였으며, 대부분의 marker에서 MSI양성인 MSI-H종양은 없었다.MSS종양과 MSI-L종양은 흡연력, 병기, 폐암 조직형 및 LOH 빈도의 유의한 차이가 없었다. 결론:비소세포폐암에서 MSI는 비교적 흔히 관찰되지만 MMR 유전자의 불활성화에 의한 MMP pathway 는 비소세포폐암의 주요 발생기전은 아닐 것으로 생각된다.향 후 비소세포폐암의 발암과정에 있어서 MMP pathway의 역할을 규명하기 위해서는 보다 많은 예를 대상으로 한 연구가 필요할 것으로 생각되며, MSI 발생기전에 관한 추가적인 연구가 필요할 것으로 생각된다. Purpose: Microsatellite instability(MSI) is frequently used as an indicator of microsatellite mutator phenotype (MMP) tumors.MSI has been observed in a percentage of non-small cell lung cancer(NSCLC).However, its role in tumorigenesis of NSCLC remains unknown.The frequency and partten of MSI in NSCLC were evaluted and clinical parameters of MSI-positive tumors with those of MSS(microsatellite stable) tumors were compared. Materials and Methods: Twenty surgically resected NSCLCs were analyzed for 15 microsatellite markers located at chromosomes 3p and 9p.The peripheral blood lymphocytes of patients were used as the source of the normal DNA. Results:1) of 20 cases, 8(40%) demonstrated MSI. 2) Instability was observed more frequently in tri- and tetra-nucleotide repeats that in dinucleotide repeats. In all cases, instability appeared as a shift of individual allelic bands. 3) LOH was observed in 10(50%) of 20 tumors analyzed. 4) Of 20 cases, MSI-H tumor(showing MSI in the majority of markers) was absent. There were 5 MSI-L tumors( showing MSI in a greater than 10% of markers). 5) No significant difference was observed between MSI-L tumors and MSI negative tumors in terms of clinicopathologic features such as pack-year history of smoking, histologic subtype, and (delete) stage of disease.There was also no significant difference in the incidence of LOH in relation to the status of MSI. Conclusion: These data strongly suggest that MSI plays different roles in lung and colon cancer.MMP pathway appears to be far less important in the tumorigenesis of NSCLC, caused mainly by cigarette smoke, with little familial tendency.(tuberculosis and Respiratory Diseases 2000,48:24-32)

천안지역 일부 다중이용시설의 포름알데히드 농도에 관한 연구

전혜리(Hye-li Jeon),조수연(Su-yeon Jo),조혜란(Hye-ran Jo),조태진(Tae-Jjn Cho),박종안(Jong-an Park),손부순(Bu-soon Son) 대한환경위생공학회 2009 대한환경위생공학회지 Vol.24 No.2

This study was conducted at a Terminal, a Parking Lot, an Underground shopping center, a Funeral hall and a Hospital waiting room of Cheon An area in August 2008 to examine the concentration level of HCHO(formaldehyde). The concentration level of the remodeled sites were 39.4 ± 12.0 ㎍/㎥ in the terminal, 32.7 ± 7.3 ㎍/㎥ in the parking, and 97.8 ± 29.4 ㎍/㎥ in the underground shopping center. The underground shopping center had the highest level and followed by the terminal and the parking. On the contrary, the site remodeling had the level of 17.7 ± 12.4 ㎍/㎥ in the funeral hall and 17.9 ± 0.6 ㎍/㎥ in the hospital waiting room. The funeral hall had highest level than that of the hospital waiting room. Both the remodeled sites and the not remodeled site had concentration level of HCHO below the standard level, but it was higher in the remodeled sites. There were no relations among the HCHO, the temperature and the humidity in facilities of Cheon An area.

제주도 연안해역의 해황 및 기상이 어획생산량의 변동에 미치는 영향

손태준,박원배,노홍길,양성기 제주대학교 1992 논문집 Vol.34 No.-

. Long-term time series data of water of water temperature and surface meteorological elements are used to investigate effects on the fishery production in the neghboring seas of Cheju island. Long-term(more then 12 months) and the seasonal variations between the sea surface temperature and the air temperature are highly correlated excepting the phase difference by the unusual cold period. Also the ocean conditions in the shallow water of Cheju island are easily affected by meteorological conditions and have a large variation in comparison with the often ocean. The sea conditions in the near seas of Cheju island are related to the fishery productions are increased. The fishery productions by the set-net are shown the maximum value in the Fall, radiating from the ocean to the air, and it was shown that the optimum temperature was 19℃, salinity 32.0-32.6 and density 22.6-23.2. The maximum catches of fish were appeared to be related to the times before and after the passing of typhoon or of a cyclone, the sea surface temperature decreased, turbidity was increased and fishery procuction was significantly increased.

농산물 소독과정에서 브롬화메틸에 노출된 근로자에게 발생한 신경병 2례

박태혁,김정일,손지언,김종국,김형수,정갑열,김준연 大韓産業醫學會 2000 대한직업환경의학회지 Vol.12 No.4

목적 : 농산물 소독과정에서 브롬화메틸에 노출된 근로자에게 발생한 신경병 2례 보고. 방법 : 피로감, 전신무력감, 어지럼증, 보행장애 등의 증상을 주소로 내원한 브롬화메틸을 사용하여 방역작업에 종사하는 건강한 20세와 18세의 근로자를 대상으로 혈액검사, 신경정도 및 근전도검사, 뇌자기공명영상 등의 임상검사와 작업력조사를 시행하였다. 결과 : 1례에서 뇌자기공명영상에서 연수와 소뇌의 양측 측충부의 고신호강도가 관찰되었고 11일 후의 추적조사에서 동일 부위의 고신호강토가 감소된 소견이 관찰되었으며 뇌병증으로 최종 진단되었다. 다른 1례는 신경전도검사에서 다발성 신경병 소견과 뇌유발전위검사에서 우측 하부 뇌간부위 병소가 관찰되었고 뇌병증 및 말초신경 병으로 최종 진단되었다. 결론 : 근로자들의 증상은 소독과정에서 브롬화메틸 노출로 인한 신경 병으로 판단되며 훈증소독과방역작업에 종사하는 근로자들의 실태조사와 안전에 대한 대책이 시급하다고 하겠다. Objectives : To report two cases of neuropathy due to methyl bromide intoxication. Methods : Workers, engaged in the fumigating process, complained fatigue, general weakness, ataxia, and hypersomnia. We evaluated them with blood tests, neurophysiologic studies and MRI and investigated their occupational history. Results : Increased signal intensities were found in the medulla oblongata and paraver mian of cerebellum in MRI and after 11 days, high signal intensities were reduced in the following MRI. In the other case, polyneuropathy and rlght lower brainstem lesion were observed In the NCV and BAEP studies. Conclusions : We confirmed that worker's symptoms were related to methyl bromide exposure in the fumigation. It is necessary that we should evaluate present condition of fumigating process and prepare appropriate methods to protect workers engaged in the fumigation.

癩患者의 型別에 따른 末梢血液 T림프구 亞型의 分布

孫基滉,白太鉉,朴鼎圭,崔大卿 충남대학교 의과대학 지역사회의학연구소 1988 충남의대잡지 Vol.15 No.1

It is well known that there is an imbalance of T lymphocyte subpopulation in lepromatous leprosy (LL). To determine whether this imbalance can be normalized along with the improvement of clinical symptomes, the status of T lymphocyte subsets was studied in the peripheral blood of the leprosy patients who have been treated for many years. The results were summarized as follows: l. The percentage and the total number of T lymphocytes were increased in both tuberculoid leprosy (TT) and LL patients as compared with controls (p<0.01). 2. The reduction of the percentage of helper T cells were significant in both bacteriologically positive LL (25.8%, p<O.01) and negative LL (31. 1%, p<0.05). 3. The percentage of suppressor T cell was significantly increased in both bacteriologically positive LL (31.5%, p<0.01) and negative LL (30.9%, p<0.01). 4. The reduction of T helper/supressor(H/S) ratio was significant in both bacteriologically positive LL (0.7±0.23, p<0.01) and negative LL (1.0±0.41, p<0.01), in contrast to controls (1.8±0.15). 5. The reduction of percentage of helper T cells was persisted in both bacteriologically positive LL (25.8%) and negative LL who convert to be negative within 5 years(25. 7%), but the increase were significant in negative LL who were convert to be negative in 6 to 10 years (31. 3%) and convert to be before 11 years(36.8%). 6. Bacteriologically negative LL patients still display impairment of CMI, but a few of them return to the normal value of the H/S ratio. 7. The results in patients with TT did not differ significantly from those of controls. From above results, defective CMI of LL was caused by the decrease of helper T cells and the increase of suppressor T cells. In spite of the H/S ratio progressively return to the normal value in a few patients, it is difficult to predict the clinical progression by this results because of the individual variations.

Co/Mn/Br계 촉매상에서 p-자일렌 액상산화반응의 공정변수에 관한 연구

손용배,박경린,류태공,김정희,오인석,김영호,양현수 忠南大學校 産業技術硏究所 2001 산업기술연구논문집 Vol.16 No.2

Liquid phase oxidation of p-xylene using molecular oxygen has been carried out on the Co/Mn/Br-based catalyst system in acetic acid as a solvent. Process parameters(total pressure, partial pressure of oxygen and temperature) and kinetics of the reaction were studied as a basic study in the viewpoint for the development of new catalyst system and process. The total pressure above 20atm and the partial pressure of oxygen above 5atm were required to keep the reactant in the liquid phase and to overcome the mass transfer limitation of oxygen. The oxidation rate conversion were increased with increasing reaction temperature to the 100∼190℃ range. For a given initial concentration of p-xylene, the oxidation rate and the apparent activation energy were found to be of first-order and 4.24 ㎉/㏖, respectively. The effect of the third components added to the Co/Mn/Br catalyst was also studied. It was found that Ni is only a good additive that can promote the catalyst performance.

환경문제에 관한 대학생들의 인식에 관한 연구

손부순,정용택,문정숙,박태술 한양대학교 환경및산업의학연구소 2000 環境과 産業醫學 Vol.9 No.1

Hand Ball 競技에 있어서의 Shoot에 대한 分析

朴淳哲,孫泰烈 成均館大學校 1980 論文集 Vol.27 No.-

Purpose The main purpose of this study is to analysis of the shooting tendency in distance, angle, and form in Hand Ball game. Subjects To this end, the resercher has selected 35 games from the participating teams including 9 teams of male college, 9 teams of Female college……, and 8 teams of male high school, 9 teams of female. high school in the 60th National Sports Festival Hand-Ball Came held in Dae-Jun October 1979. Methods The researcher of this study classify the measurement into the following categories; 1. Distsnction of the Shooting Distance 2. Distinction of the Shooting Angle 3. Distinction of the Shooting Form Results The Results May Summarized as Follows; 1. The times of shooting; Male College 746 Female college 654 Male High School 597 Female High School 429 2. The Point rate of the shooting; Male College 47.31% Female College 45.56% Male High School 46.90% Female High School 42.65% 3. The point rate of the penalty-throw; Victoried Game's M. 72% Defeated Game's M. 55%

토착형 P. vivax에 의한 Malaria 4례

박형렬,이성원,이경미,김태겸,한진영,손춘희,정원태,김성은,김종성 동아대학교의과대학 1997 동아의대학술지 Vol.9 No.2