들잔디 성숙종자로부터 캘러스배양 및 식물체 재분화에 미치는 몇 가지 요인의 영향

이상훈,김범수,원성혜,조진기,김기용,박근제,성병렬,이효신,이병현 Plant molecular biology and biotechnology research 2004 Plant molecular biology and biotechnology research Vol.2004 No.-

In an effort to optimize tissue culture responses of zoysiagrass (Zoysia japonica Steud.) for genetic transformation, factors affecting callus induction and plant regeneration were investigated. MS medium containing 3 ㎎/L 2,4-D was optimal for embryogenic callus induction from mature seed. The plant regeneration frequency of 73.3% was observed when embryogenic calli induced in this medium were transferred to N6 medium supplemented with 0.1 ㎎/L 2,4-D and 5 ㎎/L BA. Among several basic media, MS and N6 medium were optimal for callus induction and plant regeneration, respectively. Regenerated plants were grown normally when shoots transplanted to the soil. A rapid and efficient plant regeneration system established in this study will be useful for molecular breeding of turfgrass through genetic transformation.

메스암페타민 사용 환자의 정신과적 증상에 영향을 미치는 요인

이춘우,권성민,조성남,권도훈,임효덕,이성국 대한생물치료정신의학회 2004 생물치료정신의학 Vol.10 No.1

목 적 : 본 연구는 메스암페타민 남용 또는 의존 환자의 사회인구학적 특성과 이에 따른 동반된 정신장애를 알아보고, 메스암페타민 사용이 정신과적 증상에 미치는 영향을 알아보기 위함이다. 방 법 : 2002년 7월부터 2003년 7월까지 국립부곡병원 약물중독진료소에 입원한 환자 중 DSM-Ⅳ 진단 기준에 의하여 메스암페타민 남용 또는 의존으로 진단된 44명의 남자 환자를 대상으로 사회인구학적 변인과 물질 의존의 병력, SCL-90-R, BPRS, HRSD, YMRS, Impulsive scale 등을 측정하였다. 결 과 : 메스암페타민 사용 환자들의 93.2%가 우울하였으며, 59.1%는 치료가 필요한 정도의 우울증을 가지고 있었다. 20대 연령층에서는 대인예민성, 적대감 및 공포증에서 유의하게 높았고, 중졸 이하의 교육 수준에서는 대인예민성, 충동성에서 유의하게 높았으며, 무직인 경우는 강박증, 불만, 적대감 및 조중 척도에서 유의하게 높았다. 처음 사용한 연령은 사용 횟수, 유병 기간과 유의한 상관 관계를 보였으나, 수감 횟수와는 낮은 상관 관계를 보였다. 사용 횟수와 적대감, 공포증, 편집증은 서로 유의한 정상관을 보였다. 우울증 척도와 정신병 척도 및 충동성 척도는 서로 유의한 정상관을 보였다. 100회 미만보다 500회 이상 사용했을 경우에 불안, 적대감, 공포증 및 편집증에서 유의하게 높았다. 결 론 : 메스암페타민 남용 또는 의존 환자에서 우울증 유병률이 매우 높게 나타났고, 20대 연령층과 중졸 이하의 교육 수준 그리고 무직인 경우 정신과적 증상이 많이 동반되었으며, 사용 횟수에 따라 불안, 적대감, 공포증, 편집증에서 유의한 차이를 보였다. 그러므로 모든 메스암페타민 사용자에 대해 적절한 정신과적 평가가 필요할 것으로 생각한다. Objectives : The purpose of this study was to examine the sociodemographic characteristics and the comorbid psychiatric disorders and the effect of methamphetamine use on psychiatric symptoms in methamphetamine abuse or dependent patients. Methods : The study subjects were 44 hospitalized male patients who met the DSM-Ⅳ criteria for methamphetamine abuse or dependence. The study included items assessing the sociodemographic profiles, history of substance use, and psychiatric symptomatology. Psychiatric assessments were based on SCL-90-R, BPRS, HRSD, YMRS and Impulsive scale. Results : In methamphetamine use patients, 93.2% were depressed and 59% had moderate to severe depression There were significant differences in interpersonal- sensitivity, hostility and phobia in the 3rd decade age group, interpersonal-sensitivity and impulsive scale in .under the middle school education group, and obsession-compulsion, anxiety, hostility and. YMRS in no-job group, respectively compared to the other groups. Age of the first use was correlated with the frequency of use, and also with the duration of illness, but not with the number of incarceration. There were positive correlations among HRSD, BPRS and impulsive scale. In the above 500 times used group there were significant differences in anxiety, hostility, phobia and paranoia compared to the below 100 times used group. Conclusion : In methamphetamine abuse or dependent patients, the incidence rate of comorbid depression was 93.2%. In the 3rd decade age group, under the middle school education group and no-job group showed higher diverse psychiatric symptoms compared to the other groups. Frequency of use was positively correlated with the symptoms such as anxiety, hostility, phobia and paranoia. We suggest that relevant psychiatric evaluations are needed for all methamphetamine users.

다산부에서 자간전증의 임상적 고찰

이주롱,이석민,한효상,이해혁,이임순,이권해 순천향의학연구소 2001 Journal of Soonchunhyang Medical Science Vol.7 No.2

목적 : 전자간증 산모에서 초임신부와 다산부 각각의 임상적 특징을 조사하여 비교 분석하고자 하였다. 연구방법 : 1998년 1월부터 1999년 12월까지 2년간 순천향대학 서울병원에서 입원 분만한 임신성 고혈압성 질환 환자중 초임신부 106명과 다산부 50명, 총 150명을 대상으로 임상통계학적으로 조사 분석하였다. 결과 : 분만까지 임신기간의 비교시 초임신부에서는 33-36주에서 17.9%였고 다산부에서는 34.0%로, 다산부에서 조기분만의 예가 의의있게 많았다. 신생아 체중이 2500g 미만인 군이 초임신부군에서는 36.8%였고 다산부군에서는 62.0%로 다산부군에서 더 많았다. 임신성 고혈압성 질환의 중등도를 보면 중증자간전증의 초임신부에서 35.8%와 다산부에서는 44.0%로, 초임신부에 비해 다산부군의 중증 자간전증의 비율이 많았다. 결론 : 중증 임신성 고혈압성 질환이 다산부군에서 더 많았으며 적은 주수에서 제왕절개술을 시행해 출생아의 체중이 초임신부군에 비해 더 작았고 Apgar 점수도 더 낮았다. Objective : To study the clinical differences of primiparous and multiparous preeclamptic patients. Methods : We statistically analyzed 106 primiparous and 50 multiparous preeclamptic patients who were admitted to the Soonchunhyang University Hospital from January 1998 to December 1999. Results : It was statistically found that multiparae were more likely to give premature birth than primiparae: Thirty four percents of multiparae gave birth in the period between the 33rd and the 36th gestational weeks while 17.9% of primiparae did in the same period. Sixty two percents of the newborn babies of multiparae weighed less than 2500g. Among primiparae, the ratio was 36.8%. In addition, more multiparae(44.0% vs. 35.8%) suffered from severe preeclampsia. Conclusion : severe preeclampsia was found more frequently among multiparae. Since they gave birth earlier by cesarian section than primiparae, their babies weighed less and had lower apgar scores.

요골동맥을 이용한 관동맥 조영술의 유용성

윤정한,이승환,이한요,김장영,김일회,최윤종,이형준,이명옥,김승년,황성오,홍인수,최경훈 대한순환기학회 1998 Korean Circulation Journal Vol.28 No.10

위암과 식도암이 병발된 다발성 원발성 악성종양 1예

박사영,이나영,이효진,이선영,최진혁,이순남,심강섭,성순희,한운섭 梨花女子大學校 醫科大學 醫科學硏究所 1996 EMJ (Ewha medical journal) Vol.19 No.3

Multiple primary cancer means that more that two cancers occur independently in an individual. Recently, the incidence of multiple primary cancer has increased with lengthened survival, of cancer patients, development of new diagnostic technique and increased clinical evaluation. We report a patient who had adenocarcinoma of stomach combined with squamous cell carcinoma of esophagus simultaneously.

Novel Methylation Biomarker for Non-invasive Diagnostics in Lung Cancer

오태정,( Chang Hun Lee2 ),( Min Ki Lee ),( Yeul Hong Kim ),( Sang Yull Lee ),( Hyo Sung Jeon ),( Shin Yup Lee ),( Seung Soo Yoo ),( Jae Yong Park ),( Sung Whan An ) 대한결핵 및 호흡기학회 2012 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.114 No.-

To identify aberrantly hypermethylated DNA in lung cancer cells we established a genome-wide analysis for hypermethylation sites, namely Methyl DNA Isolation and Amplification (MeDIA) coupled-CpG microarray analysis. In the comprehensive methyaltion profiling analysis between human lung cancer, A549 cells and normal NHBE cells, we observed that several clusters of genes show a significant level of aberrancy in CpG island methylation pattern in cancer cells compared to normal cells. We further identified PCDHGA12 gene as a new marker of non-invasive diagnostics for lung cancer based on followings. 1) Transcription of PCDHGA12 gene is reactivated after treatment of A549 cells with demethylating agent. 2) Bisulfide clonal-sequencing reveals that CpG island of PCDHGA12 shows a distinctive differential methylation between two cell lines. 3) Pyrosequencing-based quantitative methylation assay for such region in tumor and non-tumorous tissues from lung cancer patients shows aberrant hypermethylation in 37 (92%) of the 40 tumor tissues. In clinical validation by pyrosequencingin induced-sputum of lung cancer patients (n=87) and healthy controls (n=51), we observed aberrant hypermethylation incident at significantly elevated level in samples derived from lung cancer patients. According to the optimal threshold calculated by ROC curve analysis, sensitivity and specificity of PCDHGA12 was 86.2% and 82.4%, respectively. PCDHGA12 methylation status could be a potential methylation biomarker alone or combined with others for the screen and the detection of relapse of lung cancer.

고령 장애인의 일(WORK) 경험과 욕구에 관한 연구 : 뇌병변 장애를 중심으로

이효성,김근아,김태연,오정은 한국장애인고용촉진공단 고용개발원 2007 장애와 고용 Vol.17 No.2

본 연구의 목적은 고령이면서 장애를 동시에 경험하고 있는 고령 장애인들의 일 경험과 욕구에 관한 특성을 당사자의 관점에서 이해하는 것이다. 이러한 목적을 달성하기 위해 50세 이상 뇌병변장애인 13명을 대상으로 일 경험과 욕구에 관한 개방형식의 질문으로 심층 인터뷰를 실시하여 그 결과를 분석함으로써 고령 장애인 당사자의 일 경험과 욕구에 대한 심층적 이해와 현상의 의미를 깊이 있게 파악하고자 하였다. 분석결과, 고령 뇌병변장애인들은 일을 하고자 하는 높은 욕구를 가지고 있으며 일을 통해 삶의 의미와 보람을 찾고자 하나 현실적으로는 장애와 고령, 그리고 만성질환자라는 삼중고 속에서 “일하기” 에 상당한 제약을 받는 것으로 나타났다. 이러한 연구결과는 지금까지 고용정책에서 소외되었던 고령 장애인의 ‘일’ 에 관한 정책적 개입의 필요성을 강력하게 시사해 주고 있다. The purpose of this study is to understand the characteristics of the experiences and needs for work of aged people with disabilities who experience both old age and disability from their viewpoint. To this end, this study conducted in-depth interviews with 13 people aged 50 or older with encephalopathic disabilities by open-ended questions regarding their experiences and needs for work, and analyzed the results so as to more deeply understand the experiences and needs for work of aged people with disabilities as well as the implications of the present situation. The analysis results showed that aged people with disabilities have high needs for work and try to find the meaning and worth of life through work, but in reality face considerable limitations to ‘working’ in the triple handicaps of disability, old age, and chronic disease. The results of this study strongly suggest the need for policy intervention in the ‘work’ of aged people with disabilities who have been neglected by employment policies until now.

(Bi_2O_3)_0.73(CaO)_0.27 세라믹 산소이온전도체의 저온에서의 전기전도 특성

李晟齊,盧鎭孝,白鉉德 홍익대학교 산업기술연구소 1996 産業技術 Vol.6 No.-

Low temperature characteristics of ionic conduction in (Bi_2O_3)_0.73(CaO)_0.27 ceramics were investigated by ac impedance analysis. In the 200 to 500℃ temperature range grain boundary effect was not separated in the impedance plane. The speciments with larger grain size exhibited higher electrical conductivity and lower activation energy than deternined to be 101 kJ/mol, which was very close to the reported value, 106 kJ/mol. At 300℃ and lower temperatures conductivity curve bent toward higher conductivity, as was reported in the some other ion conducrors. The conductivity showed aging effect at temperatures lower than 350℃, but conductivity decrease was not significant.

Ar 이온에 의해 Sputtering된 Pt(111) 표면의 NO 흡착

이순보,부진효,이성용,박종윤,곽현태 성균관대학교 기초과학연구소 1992 論文集 Vol.43 No.1

Adsorption of nitric oxide on the Pt(111) surface sputtered by Ar-ion has been studied using thermal desorption spectroscopy, Auger electron spectroscopy, and low energy electron diffraction. A thermal desorption spectrum obtained from the perfect Pt(111) saturated with NO at 300K is quite simillar to those reported previously. The main portion desorbs at about 370K(α-state) with a shoulder at about 470K(β-state). The chemisorption of nitric oxide is predominantly molecular on the Pt(111) surface, accompanied by a small amount of dissociation which becomes negligible when the Pt(111) surface is perfect. When the Pt(111) surface is sputtered by Ar-ion with 2KeV, the thermal desorption spectrum becomes quite complex. The shoulder peak, which appears on the perfect surface spectrum, increases with Ar-ion sputtering time. The maximum desorption spectra of N₂ and N₂O are observed simultaneously between 470 and 600K. The desorption mechanisms for N₂O are proposed. The increasing N₂ with the β-state of NO indicates that the β-state is a precursor to the NO dissociation.

역형성 핍지교세포종의 임상 분석에 대한 연구

이현성,최우진,고영초,이채혁,박효일 인제대학교 백병원 2002 仁濟醫學 Vol.23 No.5

Objectives : In this study, the author reviewed the clinical features of the patients with anaplastic oligodendroglioma and evaluated the efficacy of surgery, radiation therapy and chemotherapy as a treatment modality. The study was intended to provide the standardized treatment option for this rare disease. Methods : The author present ten cases of pathologically proven anaplastic oligodendrogliomas from 1988 to 2002. A retrospective analysis of clinical presentation. radiological and pathological features, and the effectiveness of treatment was performed and the factors affecting prognosis were investigated. Fesults : The ten patients comprised of four males and six females. The mean age at diagnosis was 34.4 (8-70). The mean follow-up duration was 40.5 months. and two patents died of tumor progression during the follow-up(70, 86 months, respectively). The most common presenting symptom was seizure(70%). All patients underwent surgery and the tumors were removed as much as possible. Postoperatively, Radiation therapy was applied to seven patients. and PCV(procarbazine CCNU-vincristine) based chemotherapeutic agent was administered to five patients. Total removal was achieved in five cases and subtotal removal was possible in the rest. In the group with PCV therapy, complete remission was observed in two, partial remission, stable disease, and disease progression to death in one, respectively. The patients well tolerated the toxicity of chemotherapy. During the follow-up, tumor recurrences were observed in four patients, and they were treated with additional operations and/or chemotherapy. Mixed anaplastic oligoastrocytoma showed earlier recurrence than anaplastic oligodendrogliomas in this study(P=0.014), but the other clinical factors and treatment modalities were not related to the time of recurrence of the tumor. Conclusions : In this study, the authors confirmed the good response of postoperative radiological treatment and chemotherapy in patients with anaplastic oligodendroglioma. PCV chemotherapy should be considered as primary treatment method for the patients with anaplastic oligodendroglioma.