플라즈마 표지소자의 제작

이상윤,라병욱,박동수,황인헌,이덕동,신영남,박성배,이동욱,박용석,박형근,손상호,권태근,채경락,정경득 慶北大學校 自然科學大學 1986 自然科學論文集 Vol.4 No.-

An Ac-type Plasma Display Panel (PDP) operating with Ne-Ar(0.1%) Penning mixture gas is fabicated. The characterics of the panel with electrodes covered with thin and thick dielectric layers are studied. The brightness of the Neon-orange light emitted by the panel measured as function of applied voltage and frequency. As an application, a graphic display system equipped with PDP showing still and moving pictures is made.

연작업자들의 신기능 평가에서 요중 δ-aminolevulinic acid 측정의 의의

이병국,이숙,김용배,리갑수,김화성,황규윤,장봉기,이성수,안규동 순천향대학교 산업의학연구소 1999 순천향산업의학 Vol.5 No.1

To clarify the effect of occupational lead exposure on renal function 241 lead workers working and 56 non lead workers were studied in 5 lead acid battery industries. Study variables for renal effect were urinary N-acetyl-β-D-glucosaminidase(NAG), urinary total protein(U-TP), blood urea nitrogen(BUN), serum creatinine(S-Cr), and serum uric acid(S-Ua). On the other hand, blood lead(PbB), zinc protoporphyrin in whole blood(ZPP), and delta-aminolevulinic acid in urine(δ-ALA) were selected for the variables of lead exposure. Information on age, work duration, smoking and drinking history were also obtained. The results obtained were as follows. While the mean values of lead exposure indicators of lead workers were significantly different from non-exposed ones, the mean values of BUN, U-TP and S-Cr of renal function indicators of exposed were also different from non-exposed. BUN, U-TP and NAG showed statistically significant correlation with PbB and ZPP, and NAG revealed also statistically strongly significant correlation with δ-ALA. On multiple regression using renal fuction indicators as a dependent variable and δ-ALA, age, work duration,lead exposure,smoking and drinking as independent variables, only δ-ALA contributed to NAG.

급성골수성 백혈병에서 CD34와 P-당단백의 발현

이제환,김우건,김상위,이정신,이규형,장대영,최종수,김상희,김성배,서철원,지현숙 울산대학교 의과대학 1995 울산의대학술지 Vol.4 No.1

Backgrounds : The expression of the MDR-1(multidrug resistance)encoded P-170 glycoprotein(p-170)and CD34 have been well known to be associated with drug resistance in AML(acute myelogenous leukemia). P-170 and CD34 expression in AML have been reported as unfavorble prognostic parameters separately. Methods : P-170 glycoprotein expression was analyzed in correlation with CD34 expression and clinical response in 15 consecutive patients with de novo acute myelgenous leukemia (AML). They were measured with flow cytometry after direct and indirect immunofluorescence staining simultaneously. Results : 1) The positive rate of P-glycoprotein and CD34 were in two of 15 patients(13%), seven of 15 patients(46%), respectively. 2) One of two P-170 positive patients as compared with 7 of 13 P-170 negative patients achieved a complete remission(CR), which showed no clinical significant difference. 3) There was no significant correlation between P-glycoprotein and CD34 expression(r=0.29, p=0.28). 4) In de novo acute myelogenous leukemia, there was no case which expressed both P-170 and CD34 simultaneously. 5) P-glycoprotein and CD34 were not expressed in acute promyelocytic leukemia group. 6) Cytogenetic abnormalities did not show any significant difference in the rate of P-glycoprotein expression, CD34 expression and complete remission. Conclusion : CD34 and P-glycoprotein in acute myelogenous leukemia were independent parameter in this study. Further investigations are warranted for clinical implication.

납 노출자에 ALAD 및 VDR 유전자의 다형성이 혈중 납과 혈압과의 관련성에 미치는 영향

리갑수,황보영,김용배,김화성,이성수,안규동,이병국 순천향의학연구소 2004 Journal of Soonchunhyang Medical Science Vol.10 No.1

To evaluate the effect of ALAD and VDR polymorphism on the relationship of blood lead with blood pressure in lead exposed workers, 801 lead exposed workers were selected. Blood lead was selected as parameters of lead dose and systolic and diastolic blood pressure using Random Zero Sphygmomanometer were chosen as blood pressure. ALAD and VDR genotype of each subject was assayed. Demographic information such as sex, age, education, past and family history, and personal habit of smoking and drinking were collected. Job duration of lead exposed workers was also obtained. The means of age and work duration of the subjects were 40.4±10.1 years and 8.2±6.5 years. The means of systolic blood pressure and diastolic blood pressure of the subjects were 123.2±16.3 mmHg and 75.8±11.9 mmHg. The overall prevalence of hypertension was 9.7%. ALAD2, the variant allele, in lead exposed workers was 10% and ALAD1 was 90%. For VDR genotype, the Bb or BB genotypes were 11.2%, while the bb genotype was 88.8%. The mean blood lead of subjects was 25.4±10.9 ㎍/㎗ (range 8.0-65.6). In the multiple regression analysis, blood lead was the significant positive predictor of systolic blood pressure and not the significant negative predictor of diastolic blood pressure after adjusting for possible confounders (age, sex, body mass index, use of anti-hypertensive drugs, drinking and smoking habits, VDR and ALAD genotypes. Also the subjects with the VDR Bx had 2.9 mmHg higher than those with the VDR bb (p=0.09). There was no interaction of VDR and ALAD genotypes between blood lead and systolic blood pressure. In multiple logistic regression analysis, the Odd's ratio of VDR genotype(BB or Bb vs. bb) for hypertension was 1.7 (95% confidence interval: 0.8-3.2). This study suggests that blood lead increases systolic blood pressure controlling covariates.

슬러지 재자원화에 관한 연구

이주성,공성호,서승원,배성렬,김영채,이기철 漢陽大學校 環境工學硏究所 1997 環境科學論文集 Vol.18 No.-

이 연구는 1993년도에 우리나라의 연간 슬러지발생량이 약 3500만m³에 이르고 있으며, 이들 대부분이 매립되고 있다. 그러나 매립부지의 확보가 점점 어려워 지고 있으며 아울러 매립처분비용도 상승하고 있기 때문에 oil화, 퇴비화등으로 재자원화 하는 방법과 소각후의 소각회를 유효이용하는 방법 등이 연구 개발되고 실용화되고 있다. 슬러지의 oil화는 현재 연구단계에 있으나 앞으로 슬러지의 유효이용기술로서, 가장 좋은 방법이 되리라 기대되고 있다. 그러나 소각후의 소각회의 이용방법은 현재 일본등에서는 실용화 되어 건설자재 등으로 다양하게 이용하고 있다. 우리나라에서도 서울시 등을 비롯한 대도시에서는 하수처리장에서 발생하는 슬러지를 소각한 후에 소각회를 건설 자제용으로서 지자체의 공사에는 반드시 사용하도록 하는 제도를 만든다면 유효이용방법으로 충분한 타당성이 있을 것이다. 이때 소각방법으로는 용융소각방법이 보다 효과적일 것이다. With increasing sewage and wastewater sludge, it has become difficult to dispose the sludge by landfilling and ocean dumping. To solve this problem, efforts have been made to delelop sewage and wastewater sludge utilization technologies in many advanced countries. Some technologies have already been developed; conversion of sludge to compost and fuel, producing artificial light-weight aggregate from ashes. This paper describes the technical status of sludge utilization technologies.

납 폭로 근로자에서 δ-aminolevulinic acid dehydratase 유전형질이 zinc protoporphyrin 및 δ-aminolevulinic acid에 미치는 영향

이병국,황규윤,장봉기,김용배,리갑수,안현철,김화성,이성수,안규동,박종범 순천향대학교 산업의학연구소 1999 순천향산업의학 Vol.5 No.1

The inhibition of δ-aminolevulinic acid dehydratase (ALAD) in the heme synthetic pathway results in increased protophoryphyrin (ZPP) and δ-aminolevulinic acid (ALA) and is responsible for some of toxicological effects of lead. This enzyme is coded by the ALAD gene containing 2 co-dominant alleles. The polymorphisms of ALAD gene may be related to differential effects of lead on ZPP and ALA, ALAD genotype was measured in 975 Korean male lead workers, of whom 897 were homozygous for ALAD1 (ALAD 1-1 genotype) and 96 were heterozygous for ALAD2 (ALAD 1-2 genotype). Blood lead in subjects with ALAD1 was significantly higher than those with ALAD2 (p = 0.01). No difference between ALAD genotypes was found for age, exposure duration, ZPP, ALA, hemoglobin, hematocrit, body mass index, tobacco and alcohol use. After adjustment for possible confounders, ALA and ZPP became significantly elevated in ALAD1 subjects (p = 0.004 and 0.055, respectively). This result suggests that ALAD1 subjects may be more susceptible to the hematotoxicologic effects of lead than ALAD2 subjects.

동종골수이식 후의 이식편대숙주 질환의 빈도와 임상양상 : 단일 병원 치료 경험 A Single Institution Experience

이규형,이제환,이정신,서철원,김상위,김성배,김정균,김신,김우건,김상희 대한조혈모세포이식학회 1997 대한조혈모세포이식학회지 Vol.2 No.1

배경: 이식편대숙주 질환은 동종골수 이식후 발생할 수 있는 가장 중요한 합병증의 하나이다. 일반적으로 이식편대숙주 질환이 서구에 비하여 한국을 비롯한 아시아 국가에서 드물다고 알려져 있다. 방법: 저자들은 1993년 12월부터 1997년 6월까지 동종골수이식을 받은 전체 44명의 환자를 대상으로 급성 및 만성 이식편대숙주 질환의 빈도와 임상 양상을 조사하여 분석하였다. 대상환자중 남자가 27명 (61%) 이었고, 여자가 17명 (39%)이었다. 중앙연령은 33.5세 (범위, 16-47세)였다. 17명은 급성골수성 백혈병, 6명은 급성림프구성백혈병, 11명은 만성골수성백혈병, 8명은 중증재생불량성빈혈, 1명은 골수이형성증후군, 그리고 나머지 1명은 신경아세포종(neuroblastoma) 환자였다. 이식편대숙주 질환 예방으로 methodtrexate와 cyclosporine복합요법을 시행하였다. 결과: 3명에서 급성 이식편대숙주 질환이 이식후 18일에서 48일 사이에 관찰되었다(빈도, 7%). 급성 이식편대숙주 질환정도는 3도, 2도 그리고 1도가 각 한명씩이었다. 4도 급성 이식편대숙주 질환은 관찰되지 않았다. 13명의 환자가 만성 이식편대숙주 질환을 경험하였다(30%). 이들 중 10예는 제한성 병변이었고 3예는 전신성 병변이었다. 만성 이식편대숙주 질환의 주된 발병 증상 또는 징후는 황달/간기능 이상(8예)과 구강점막병변(4예)이었다. 한 명의 환자가 이식 후 107일에 간 이식편대숙주 질환에 의한 간부전으로 사망하였다. 결론: 한국인 환자에서는 동종골수이식 후 급성 및 만성 이식편대숙주 질환의 빈도가 구미의 환자에 비하여 상대적으로 낮고 그 정도도 덜한 것으로 보인다. 한국인 환자에 가장 적합한 이식편대숙주 질환 예방 방법의 정립을 위한 계속적인 연구가 필요하다고 사료된다. Background: Graft-versus-host (GVHD) disease is one of the most important complications after allogeneic bone marrow transplantation (BMT). Generally, it has been known that graft-versus-host disease is less frequent after allogeneic BMT in Asian countries including Korea when compared to western countries. Methods: We investigated the frequency and clinical pattern of acute and chronic GVHD in 44 consecutive patients who underwent allogeneic BMT between December 1993 and June 1997. There were 27 male (61%) and 17 female (39%) patients with median age of 33.5 years (range, 16-47). Seventeen patients had acute myelocytic leukemia (AML), 6 acute lymphocytic leukemia (ALL), 11 chronic myelocytic leukemia (CML), 8 severe aplastic anemia (SAA), 1 myelodysplastic syndrome, and 1 neuroblastoma. Methotrexate/cyclosporine combination was given for acute GVHD prophylaxis. Results: There patients (7%) developed acute GVHD median 31 days (range, 18-48) after BMT. There were one grade 3 acute GVHD involving the liver. There was no patient with grade 4 acute GVHD. Thirteen patients (30%) developed chronic GVHD median 100 day (range, 53-266) after BMT. Ten cases were limited stage and 3 were extensive stage. Frequent initial manifestations of chronic GVHD were jaundice/liver function abnormality in 8 cases and oral mucosal lesion in 4 cases. One patient died due to liver failure which were felt to be seconday to chronic GVHD 107 days after BMT. Conclusion: Acute and chronic GVHD are less frequent in Korean patients after allogeneic BMT when compared to historical data from patients from western Europe or United States. In cases of chronic GVHD, mild forms of disease predominate. Optimal strategy for GVHD prophylaxis in Korean patients remains to be defined.

새로운 연노출의 효과지표로서의 혈장 δ-aminolevulinic acid 측정의 유용성

이금원,황보영,김용배,리갑수,이성수,장봉기,함정오,안규동,이병국 순천향대학교 산업의학연구소 2001 순천향산업의학 Vol.7 No.1

To evaluate the usefulness of the measurement of plasma δ - aminolevulinic acid (ALAP) as a new effect indicator of lead exposure, the association of ALAP with blood lead, DMSA chelatable lead and bone lead with a special emphasis of genetic interaction of ALAD polymorphism was studied with 419 lead exposed workers and 85 non-lead exposed workers. Lead workers were recruited from storage battery industries, secondary smelting industries and other lead using industries and non-lead workers selected from electronic refrigerator manufacturing industry. Blood lead, DMSA chelatable lead and bone lead were chosen for parameters of lead exposure. Plasma and urinary ALA were selected for the effect parameter of lead intoxication. Information for age, smoking, drinking habit and body mass index were also obtained. Job duration for lead workers was also collected. The overall prevalence of the variant allele, ALAD type 1-2 or 2-2(ALAD2) in lead exposed workers was 10.0% (42 out of 419 lead workers) which was not differed from control workers (9.4% : 8 out of 85). The means blood lead and DMSA chelatable lead of lead workers with ALAD 1(ALAD type 1-1) were lower than those of lead workers with ALAD2, but the differences were not statistically significant. On the other hand, the mean of tibia lead of lead workers with ALADI was higher than that of lead workers with ALAD2, but the difference was not statistically significant. DMSA chelatable lead showed highest correlation with log transformed plasma ALA(LALAP)(r=0.682) and blood lead and tibia lead had r=0.650, r=0,402 correlation coefficient with LALAP respectively On the other hand. log transformed urinary ALA(LALAU) had lower correlation coefficient with all three parameters of lead exposure than LALAP. The equation of robustic simple linear regression of blood lead on LALAP was LALAP=2.0439+0.0165PbB in ALADI lead workers and LALAP=2.031+0.0121PbB in ALAD2 lead workers. ALAD genotype in the multiple regression analysis of blood lead with LALAP after adjusting for possible confounders(sex, ago, BMI, smoking and drinking status and job duration) showed significant main effect on LALAP resulting lower LALAP in ALAD2 lead workers than ALADI lead workers. On the other hand, effect modification of ALAD genotype was observed in the multiple regression analysis of tibia lead with LALAP after adjusting for possible confounders, but there was no significant effect of ALAD genotype in the multiple regression analysis of DMSA chelatable lead with LALAP after covariate adjustment. With above results, plasma ALA was found to be a useful effect parameter of lead exposure compared with urinary ALA. It was also observed that ALAD polymorphism made significant effect on the association of blood and tibia lead with ALAP. Thc lower plasma ALA in ALAD2 lead workers suggested supportive effect of ALAD2 in lead exposure.

韓國産 자작나무科의 系統分類學的 硏究(序論)

李相泰,蘇雄永,田寬培,李愚喆 全北大學校 生物學硏究所 1981 生物學硏究年報 Vol.2 No.-

The present paper is an introduction to the whole project of "The systematic studies on Korean Betulaceae". Three studies under the project were performed this year and presented in this report : a review of taxonomic literatures and the construction of distribution maps by Woo-Tchul Lee, pollen morphology by Sang-Tae Lee and Moo-Yeol Kim, and wood anatomy by Woong-Young Soh and Kwan-Bae Jeon.

업종에 따른 연 취급 사업장의 기중 연 농도 및 연 노출 수준 평가

이병국,김용배,리갑수,안현철,김화성,이용진,황규윤,장봉기,이성수,안규동 순천향대학교 산업의학연구소 1999 순천향산업의학 Vol.5 No.1

In order to obtain an useful information for health management and biological monitoring of lead exposed workers, Authors tried to investigate air lead level and the lead exposed level of workers in lead industry according to occupational category. The subjects in our study were 2074 workers in 7 lead-using industries, and study subjects were divided into 4 occupational categories such as storage battery industry (type 1), primary smelting industry (type 2), secondary smelting industry (type 3) and litharge making industry (type 4). Blood zincprotoporphyrin concentration (ZPP), blood lead concentration (PbB) and urinary δ -aminolevulinic acid (ALAU), hemoglobin (Hb), hematocrit (Hct) were selected as the indices of lead exposure. Personal variables such as age, work duration were also collected. The results were as follows. 1. The geometric mean air lead in 9 lead-using industry was 0.1133±4.3120 ㎎/㎥, and that in type 1, 2, 3 and 4 was 0.1038±3.4952 ㎎/㎥, 0.0429±3.4329 ㎎/㎥, 0.1877±2.5123 ㎎/㎥ and 0.9961±5.2910 ㎎/㎥, respectively. 2. The mean ZPP in 9 lead-using industry was 53.1±28.0 ㎍/㎗, and that in type 1, 2, 3 and 4 was 52.0±24.8 ㎍/㎗, 48.0±16.4 ㎍/㎗, 109.8±85.6 ㎍/㎗ and 74.3±37.8 ㎍/㎗, respectively. There was significant difference in ZPP according to occupational category (P<0.01). The percents of lead workers whose ZPP were above 100 ㎍/㎗ in type 1, 2, 3 and 4 were 4.0%, 1.7%, 34.3% and 21.6%, respectively. 3. The mean PbB in lead-using industry was 26.0±11.2 ㎍/㎗, and that in type 1, 2, 3 and 4 was 25.3±11.1 ㎍/㎗, 26.7±8.8 ㎍/㎗, 50.3±15.2 ㎍/㎗ and 36.4±11.0 ㎍/㎗, respectively. There was significant difference in PbB according to occupational category (P<0.01). The percents of lead workers whose PbB were above 40 ㎍/㎗ in type 1, 2, 3 and 4 were 10.4%, 7.7% 71.9% and 43.2%, respectively. 4. While the correlation of Hb corrected PbB with ZPP was higher than non corrected PbB, the correlation of log-transformed ZPP with PbB was higher than non corrected ZPP. 5. Simple linear regressions of PbB and corrected PbB as independent variable with ZPP, log-transformed ZPP and ALAU as dependent variable were statistically significant (P0.01). Coefficient of determination of corrected PbB with other variables was higher than non corrected PbB. As the result of this study, it was suggested that reconsideration of environmental and biological monitoring program was highly recommended for secondary smelting and litharge making industry.