혈관 생성 억제 제를 투여한 신생마우스 폐 조직에서 Retinoic acid의 세포자멸사의 억제

주선영 ( Sun Young Ju ),조경아 ( Kyoung Ah Cho ),유경하 ( Kyung Ha Ryu ),우소연 ( So Youn Woo ),박은애 ( Eun Ae Park ),조수진 ( Su Jin Cho ) 대한주산의학회 2008 Perinatology Vol.19 No.1

목적: Retinoic acid는 폐포 재생에 중요한 역할을 하는 것으로 알려져 있어서 미숙아의 기관지폐이형성중의 예방에 사용되고 있다. 기관지폐이형성중의 병태생리는 미숙한 폐의 내피세포와 외피세포의 세포자멸사를 일으킴과 관련이 있다. 이에 본 연구에서 VEGFR2 억제가 신생 폐에서 세포자멸사를 증가시키는지, 또 retinoic acid의 투여가 본 폐 발달 억제 동물모형에서 세포자멸사를 억제하는지 알아보고자 하였다. 방법: VEGFR2 억제제인 SU1498을 생후3일된 마우스에 주사하고, 폐포발달이 이루어지는 그 후 10일간 retinoic acid나 위약을 주사하였다. 형태학적인 분석을 하였고, 세표자멸사를 비교분석하기 위해서 TUNEL 염색과 Annexin V을 표지자로 FACS출 시행하였다. 자멸사한 세포를 확인하기 위해서 동일초점 현미경으로 분석하였다. 결과: SU1498를 주사한 마우스에서 폐포면적과 평균폐포용적이 의미 있게 감소하였다. 세포자멸사도 약 3배 의미 있게 증가하였다. 자멸사한 세포는 내피세포와 상피세포에 분포하였다. Retinoic acid를 주사한 약 50%의 세포자멸사가 감소하였고, 형태학적으로 정상에 가까운 폐포 발달이 유지되었다. 결론: VEGFR2 억제를 하면 내피세포와 상피세포의 세포자멸사가 증가되면서 폐 발달이 저해되었다. 이어서 retinoic acid를 주사하면 세포자멸사가 억제되어 폐 발달유지에 도움이 되었다. 이러한 retinoic acid에 의한 세포자멸사의 억제가 기관지폐이형성증 예방에 기여할 것으로 추정된다. Background: Retinoic acid is known to play a role in alveolar regeneration and is used in the prevention of bronchopulmonary dysplasia (BPD) in premature infants. Many factors involved in the pathogenesis of BPD induce apoptosis of the endothelium and epithelium of the premature lung. We hypothesized that VEGFR2 inhibition would increase apoptosis in the newborn lung and retinoic acid would decrease apoptosis in our model of inhibited lung growth. Material and Methods: SU1498, a VEGFR2 inhibitor or vehicle was given to three-day-old mice. Subsequent retinoic acid or vehicle injection was given for ten days for the duration of alveolarization. Morphometric analyses were performed. Apoptosis was assessed with TUNEL staining and Annexin V staining. Co-localization of apoptotic cells with endothelial and epithelial cells was performed. Results: SUI498 injection reduced alveolar surface area and mean alveolar volume in newborn mice. Apoptosis was increased by three-fold in SU1498 injected mice. Apoptotic cells co-localized to endothelial and epithelial cells. Retinoic acid significantly reduced the degree of apoptosis by 50% in SU1498 injected mice and maintained lung development. Conclusion: VEGFR2 inhibition caused an arrest in lung development accompanied by an increase in apoptosis of endothelial and epithelial cells of the neonatal lung in mice. Subsequent retinoic acid treatment reduced apoptosis and we speculate that retinoic acid may preserve lung growth in bronchopulmonary dysplasia by inhibiting apoptosis in the neonatal lung.

서울의 Penicillinase Producing Neisseria Gonorrhoeae 발생빈도(1994)

김재홍,안진균,정성재,김영태,김중환,김시영,이석종,이홍렬,서호석,김경훈,권혁진,정우권,고우석,이용석,안필수,오준규,오용섭 대한화학요법학회 1996 대한화학요법학회지 Vol.14 No.1

Background : In recent years gonorrhoea has been panendemic and remains one of the most common Sexually Transmitted Diseases in the world, especially in developing countries. Objective & Method : For the detection of a more effective therapeutic regimen and assessing the prevalence of Penicillinase Producing Neisseria Gonorrhoeae(PPNG), Ive have been trying to study the patients who have visited the VD Clinic of Choong-Ku Public Health Center in Seoul since 1980 by means of the chromogenic cephalosporin method. Results : In 1994, 168 strains of N. gonorrhoeae were isolated, among which 109 (64,9% ) were PPNG. Conclustion : Our results suggests that the prevalence of PPNG in Seoul is still increasing.

인공 피음처리에 따른 전나무의 생장과 엽록소 함량변화에 관한 연구

우수영,이동섭,권오규 한국농림기상학회 1999 한국농림기상학회지 Vol.1 No.2

This study was conducted to compare seasonal changes of height, diameter at root collar, biomass and chlorophyll contents of Abies holophylla seedlings grown in Sangju National University Nursery under different light intensities. Four light intensities were adopted using nylon net such as 100% sunlight (1800 μmol m^-2 s^-1), 75% of sunlight (1350 μmol m^-2 s^-1), 50% of sunlight (900 μmol m^-2 s^-1) and 25% of sunlight (450 μmol m^-2 s^-1). Planting density in four light intensities was 30 cm × 30 cm. In each treatment, 100 trees were planted and height, diameter at root collar, biomass and chlorophyll contents were measured. The highest height, diameter at root collar and biomass in Abies holophylla seedlings were observed at 100% full sunlight. Lowest chlorophyll contents (Chlorophyll a, b and total) were shown at 25% of sunlight, lowest light intensity treatment in this study. Growths and chlorophyll contents of Abies holophylla seedlings were strongly associated with light intensity. Photosynthesis has to be investigated in near future because photosynthesis and chlorophyll contents were strongly related to tree growth with long periods.

Cilostazol의 항혈소판 응집작용과 sodium citrate의 농도에 따른 혈소판 응집율에 대한 연구

우수경,김영리,권광일 충남대학교 약학대학 의약품개발연구소 2000 藥學論文集 Vol.16 No.-

Cilostazol, 6- 〔4- (1-cyclohexyl- 1H-tetrazol -5-yl) butoxy〕 - 3, 4-dihydro- 2(1H)- quinolinone, is an antiplatelet/antithrombotic agent that has been used for treatment of chronic peripheral arterial occlusion. Cilostazol was studied for its inhibitory effects on platelet aggregation in vitro in rabbit plasma and in human plasma, and also for its antiplatelet effects ex vivo in rabbit after single oral administration. At time t=0, the rabbits were given a single oral dose of 14 ㎎/㎏ cilostazol. At 1, 2, 4, 8, 12, and 24 hours after drug administration, blood samples were taken for measurements of antiplatelet aggregation effect. Before the study of antiplatelet effects of cilostazol in rabbits, the effect of sodium citrate on platelet aggregation in various concentrations was studied to determine the optimal treatment. Cilostazol produced a potent inhibitory effect on platelet aggregation induced by adenosine diphosphate (ADP) in vitro. The maximum effect on antiplatelet aggregation was 14.4% and was appeared at 1 hour after drug administration. The optimum blood/anticoagulant (3.8% sodium citrate) ratio was 9 to 1, and 16 to 1 in human plasma and in rabbit plasma, respectively.

담도에 국한되어 증상을 유발한 석회쓸개즙 2 예

윤성우,이지영,하경호,이수경,한나,배효근,박선자 고신대학교 의과대학 2010 고신대학교 의과대학 학술지 Vol.25 No.2

Limy bile is a rare condition characterized by excessive precipitation of calcium carbonate in the gallbladder or bile duct. Recently we experienced two cases with obstructive jaundice in which limy bile in the common bile duct but not in the gallbladder for one case, and in the common bile duct and intra hepatic duct but not in the gallbladder for another case. The patients were treated by endoscopic sphincterotomy. Here, we report the cases and review the literature.

임상별 林間人蔘 의 生育과 最適環境에 관한 硏究 (Ⅰ)

우수영,이동섭 한국농림기상학회 2002 한국농림기상학회지 Vol.4 No.2

The best environments such as crown density, temperature, light intensity and humidity have to be identified because these factors are strongly related to the growth and several physiological characteristics. The purposes of this study are ① to collect basic data for growth, ② to identify the best growth environments. To achieve these purposes, oak, pine and mixed forest stands have been selected in this study. Forest ginseng seeds were sown in these forest four years ago. Several environmental and growth factors have been surveyed. In general, mean tree age, DBH and average height are 20-25 years old, 14-17cm and 7-9m, respectively. The growths of forest ginseng grown in oak stand are better than those of pine and mixed stands.

가중나무, 튜립나무, 양버즘나무 苗木을 오존에 露出 시켰을 때 葉綠素含量과 Glutathione Reductase 活性變化

禹秀泳,李成漢,權琦遠,李在千 한국임학회 2004 한국산림과학회지 Vol.93 No.7

이 연구의 목적은 대기오염 가운데 오존이 식물에 미치는 영향과 오존 스트레스의 매독 메카니즘에 중요한 기능을 하는 호소의 역할을 구명하기 위래서 도심에 가로수로 많이 사용하는 가중나무, 튜립나무 그리리고 양버즘나무 묘목을 150ppb의 농도로 오존에 하루 8시간, 34일 동안 노출 시켰다. 그리고 가시적 피해현상, 엽록소함량, glutathione reductase 활성을 측정하였다. 가시적인 피해현상은 야버즘나무가 가장 늦게 나타났고, 튜립나무가 가장 먼저 나타났다. 그러므로 아마도 튜립나무는 오존에 대해서 bioindicator 역할을 할 수 있을 것으로 판단된다. 엽록소 함량은 모든 수종에서 감소하는 경향을 보였다. 또한 glutathione reductase 효소 활성은 세 수종 모두 오존에 노출된 묘목의 경우 활성이 높게 나타났다. The purpose of this study was to identify the effects of ozone pollution on the 3 tree species in controlled environment. Ailanthus altissima, Liriodendron tulipifera and Platanus occidentalis seedlings were exposed in both control and ozone chambers to investigate visible damage, chlorophyll contents, and glutathione reductase (GR) activity. Ozone was fumigated 8 hours for a day with 150 ppb concentration. Liriodendron tulipifera seedlings showed early visible damage on the leaf during ozone exposure. It seemed to be one of the bioindicators of ozone pollution. Ozone exposure generally reduced chlorophyll contents on the leaf of three species. In addition, GR activities of 3 tree species exposed in ozone chamber were higher than those of control in every measurement. It is a typical compensatory strategy of stressed trees.

"Dice와 fill" 방식을 이용한 1-3 압전복합재의 제조와 횡방향 단위 크기에 따른 압전특성 평가

김영덕,김광일,정우철,김흥락,김동수 한국비파괴검사학회 2002 한국비파괴검사학회지 Vol.22 No.4

NDT나 의료용 영상장치에 응용되는 압전복합체는 일반적인 세라믹이나 고분자 압전재료에 비하여 많은 장점을 가진다. 이들 응용분야에서는 전기기계결합게수가 높아야 하고, 음향임피던스가 낮아야 한다. 그러나, 압전복합제의 횡방향 단위 크기가 조밀하지 못한 경우 횡방향으로 전행하는 판파에 의한 불필요한 진동이 표면에 발생하게 된다. 횡방향 단위 크기와 세라믹 체적비에 따른 압전 특성을 조사하기 위하여 PMN-PZT 세라믹과 Epofix 에폭시로 에폭시의 폭을 달리하면서 1-3 형 압전복합체를 제작하였다. 제작된 1-3형 압전복합체의 두께방향 진동모드의 전기기계결합계수, 음향임피던스는 각각 0.36~0.64, 9.8~22.7 MRayl로 나타났으며, 횡방향 단위크기가 줄어들수록 횡방향 모드 공진 주파수가 증가하였다. The piezoelectric composites had many adventages in comparison qith conventional piezoelectric ceramics and piezopolymers for ultrasonic transducers used in NDT and in medical ultrasonic imaging. The electromechanical coupling coeffucient should be high and the acoustic impedance should be low in these applications. However, the cross-coupling with spurious oscillations caused lateral spatial scale. The thickness mode electomechanical coupling coefficient of 1-3 type of piezoelectric composites were 0.36 to 0.64, and the acoustic impedance of them were 9.8 to 22.7 MRayl. The lateral resonance frequency of 1-3 type piezoelectric composites shifted to high frequency region with decreasing lateral spatial scale.

기계시각을 이용한 강구 자동 검사장치

김윤수,박수우,임병훈,김태균,최병재,박철영,이문락,도용태 대구대학교 정보통신연구소 2008 情報通信硏究 Vol.5 No.1

Metal balls are commonly used in mechanical elements and systems including bearings. If there is a defect in a metal ball, it can cause a serious machine failure. By this reason every balls produced are required to be inspected, and the inspection is usually done by human eyes. However, the inspection by human eyes is always with the risk of mistakes, and a need to automate the inspection process is arisen. This paper describes a design of an automatic inspection system for detecting defects on ball surface. A machine vision system is used, and design process and techniques are described in detail. 강구는 베어링을 비롯한 기계 요소 및 장치에서 널리 사용되는 부품이다. 만약 강구에 결함이 있을 시, 이를 사용하는 기계에서 치명적인 결함이 초래될 수 있다. 따라서 제조된 강구에 대해서는 전량검사가 요구되고 있고, 검사과정은 사람의 육안에 의한 검사가 주를 이루고 있다. 그런데 사람에 의한 검사에는 항상 실수의 가능성이 있으므로, 이를 자동화하는 것이 필요하다. 본 논문에서는 강구의 표면 결함을 자동적으로 검사할 수 있는 장치를 설계한다. 기계시각 장치가 사용되었고, 설계에 따른 구체적 절차와 기술들이 서술된다.

앞작물 재배와 질소 시비량의 차이에 따른 이탈리언라이그라스의 청예 수량

강영길,조남기,고영우,강민수,김용찬 濟州大學校 亞熱帶農業硏究所 1998 亞熱帶農業硏究 Vol.15 No.-

앞작물 재배와 질소시비량이 이탈리언라이그라스의 건물수량에 미치는 영향을 구명하기 위해 1997년 제주에서 콩(백운콩, 남해콩, 소백나물콩), 녹두(금성녹두, 남평녹두), 팥(충주팥), 동부(제주재래), 수수류(Pioneer 931, Pioneer 988). 옥수수(Pioneer 3525)를 기준시비하에서 재배한 후, 질소 0, 40, 80kg/ha 시용하여 1997-1998년에 재배했던 이탈리언라이그라스의 포장 건물수량, 건물율 등을 조사한 결과를 요약하면 다음과 같다. 초장, 건물수량, 건물율은 모두 앞작물재배에 의하여 유의한 영향을 받지 않았고 앞작물과 질소시비량간의 상호작용도 유의하지 않았다. 초장과 건물중은 질소시비량의 차이에 따른 큰 차이가 있었으나 건물율은 질소시비량의 차이에 빠른 차이가 거의 없었다. 초장과 건물중은 질소시비량이 0, 40, 80kg/ha에서 각각 99.5. 118.1, 120.1cm와 5.77, 7.23, 7.70ton/ha이었다. 질소이용효율은 질소 40kg/ha 시용구에서 180.8kg이었던 것이 80kg/ha 사용구에서 96.3kg로 줄어들었다. 건물수량과 질소이용효율을 고려할 때 늦가을에 파종한 이탈리언라이그라스의 적정 질소시비량은 약 80kg/ha으로 판단된다. After soybean (3 cu1tivars), mungbean (2 cultivars), cowpea, adzuki bean, maize, sorghum, sorghum × sudangrass hybrid, and Japanese millet were harvested for forage in 1997. Italian ryegrass was grown at three N rates (0, 40, and 80kg/ha) at Cheju in a 1997-1998 crop season to evaluate the effects of previous cropping and N rate on the dry matter yield of Italian ryegrass. Plant height, dry matter yield and dry matter content were not influenced by the previous cropping. There was no significant interaction between previous cropping and N rate for the three traits. Plant height and dry matter yield were significantly affected by N rate but dry matter content was not. Averaged across the previous croppings, plant heights and dry matter yields at 0, 40, and 80kg N/ha were 99.5, 118.1, and 120.1 cm and 5.77, 7 23, and 7.70 t/ha, respectively. Nitrogen use efficiency decreased from 180.8 to 96.3 kg dry matter per kg N applied as N was increased from 40 to 80 kg/ha. The optimum N rate for Italian ryegrass planted in late fall at Cheju appeared about 80kg/ha in terms of dry matter yield and N use efficiency.