생약제제인 이공산(異功散)의 Cisplatin 유도 신장독성 보호 및 항산화 효과

Lee, Kyung-Tae,Ahn, Kyoo-Seok,Chang, Sung-Goo,Oh, Soo-Myung,Jung, Jee-Chang 경희대학교 동서의학연구소 1999 INTERNATIONAL SYMPOSIUM ON EAST-WEST MEDICINE Vol.1999 No.1

Kyung-Tae Lee, Kyoo-Seok Ahn¹, Sung-Goo Chang², Soo-Myung Oh²and Jee-Chang Jung²¹College of Pharmacy, ²Oriental Medicine and ³College of Medicine, and East-West Medical Reserch Institute, Kyung Hee University, Seoul, Korea. Preventive and Antioxidative Effects of Crude Drug Preparation(E-kong-san) on Cisplatin Induced Nephrotoxicity. Proceedings of International Symposium on East-West Medicine, Seoul. 201-211, 1999. -Nephroprotective effects of a crude drug-prearation (E-kong-san) were determined from cisplatin on rabbit kidney proximal tubule and human renal cortical cells by MTT assays and sustained glucose consumption on ciplatin-induced human renal cortical tissue. Levels of creatinine and blood ure nitrogen(BUN) in serum after administration of cisplatin(0.75mg/kg,i.p.) to E-kong-san(0.75g/kg/day,p.o.)-pretreated rats were markedly lower compared to those of cisplatin-treated rats. Moreover, the administration of E-kong-san significantly inhibited the loss of body weight of cisplatin injected rts. These findings suggest that E-kong-san on the cisplatin induced nephrotoxicity led us to investigate whether the effect of this water extract was a result of anti-oxidation. E-kong-san showed strong free radical scavenger activities on 1,1-dipheny1-2picrylhydrazil(DPPH) radical and xanthine/xanthine oxidase(XOD) generated superoxide anion radical(O2-). We further studied the effects of E-kong-san on lipid peroxidation in rat liver microsomes induced by enzymatic and nonenzymatic methods. E-kong-san exhibited significant inhibition on both ascorbic acid/Fe2+and ADP/NADPH/Fe3+ induced lipid peroxidation in rat liver microsomes. Based on these results, we suggest that-E-kong-san attenuate the cisplatin induced cytotoxicity and its mechanism can be eplained by antioxidant.

당뇨병 환자에서 혈장 Thrombin-Antithrombin Ⅲ 및 Plasmin-α_2-Plasmin Inhibitor 복합체의 임상적 의의

김경욱,김은숙,정상수,윤수지,박우일,이준희,남수연,안철우,문병수,김경래,차봉수,송영득,임승길,이현철,허갑범 대한당뇨병학회 2002 Diabetes and Metabolism Journal Vol.25 No.5

연구배경:당뇨병 환자에서 혈액응고 및 섬유소용해 체계의 이상경향이 있어 그 결과로 여러 혈관합병증의 발생위험이 높다는 사실은 널리 알려져 있다. 그 기전은 아직 확실히 밝혀지지 않았으나, 고혈당으로 인한 혈장 단백질들의 비효소성 당화작용이나 산화성 스트레스로 인한 유리 라티칼 작용으로 응고항진이나 섬유소용해 활성의 저하를 유발하는 것으로 생각되고 있다. 최근 응고 및 용해인자와 그 억제자의 복합체들의 증가가 이 상태를 비교적 예민하게 반영한다고 알려져 있다. 방법:본 연구에서는 당뇨병 환자 101명과 정상 대조군 20명에서 혈장내 thrombin­antithrombin complex(TAT)와 plasmin­α₂­plasmin inhibitor complex(PIC)를 측정하여 비교하고, 당뇨병 환자에서 미세혈관 합병증과 대혈관합병증의 유무에 따른 차이와, 이미 혈관 질환의 위험인자로 알려져 있는 인자들간의 상관성을 알아보고자 하였다. 결과:1. 환자의 분포를 살펴보면 혈관합병증이 있는 군은 85명, 혈관합병증이 없는 군은 16명이었고, 평균연령은 각각 57.9±14.1세, 49.9±16.6세로 혈관 합병증이 있는 군에서 더 나이가 많았고, 체질량지수는 23.2±3.4㎏/㎡, 24.1±3.4㎏/㎡로 두 군간 유의한 차이는 없었다. 또 두 군간의 혈압 및 HbA1c, 공복혈당 및 인슐린과 C­peptide, 총 콜레스테롤, 중성지방, HDL­콜레스테롤, Lp⒜는 유의한 차이가 없었고, 미세혈관합병증이 있는 군에서 당뇨병의 유병기간이 길었다. 2. TAT 및 PIC의 농도는 정상 대조군에서는 2.8±1.2 ng/mL, 240.4±69.7 ng/mL이었고, 당뇨병 환자군에서는 9.5±22.6 ng/mL, 472.2±258.7 ng/mL이었다. TAT와 PIC 모두 당뇨병 환자군에서 정상 대조군에 비해 유의하게 증가되어 있었고(p<0.001), TAT/PIC ratio는 두 군간 차이가 없었다. 3. 당뇨병 환자의 혈관합병증에 따른 TAT 및 PIC, fibrinogen 농도는 합병증이 없는 군은 각각 4.1±2.4ng/mL, 362.2±272.0ng/mL, 322.7±102.4mg/mL으로 PIC와 fibrinogen의 증가를 보였으나, 연령을 보정한 후에는 통계학적 유의성은 없었다. 또 대혈관 합병증군에서는 각각 6.0±4.9 ng/mL, 507.4±321.6 ng/mL, 427.1±194.7 mg/dL이었으며 미세·대혈관 합병증군에서는 10.4±6.4 ng/mL, 484.8±269.7 ng/mL, 388.4±132.4 mg/dL으로 TAT의 증가를 보였으나 역시 연령을 보정한 후에는 통계학적 유의성은 없었다. 4. 미세혈관합병증군에서 HbA1c(>8%)가 높은 군의 PIC 농도가 유의하게 높았고(p=0.049), 대혈관합병증군에서 HbA1c(>8%)가 높은 군의 총 콜레스테롤 농도가 유의하게 높았다(p=0.042). 5. 총 당뇨병 환자군에서 PIC는 fibrinogen과 HbA1c와 양의 상관관계를, BMI와 음의 상관관계를 보였으며(r=0.47, 0.31,-0.25), 혈관 합병증이 없는 당뇨병 환자군에서만 TAT는 HbA1c와 양의 상관관계를 보였다(r=0.67). 결론:이상의 결과에서 혈장 TAT 및 PIC 농도는 당뇨병 환자에서 정상 대조군에 비해 의미있게 증가되어 있었고, 당뇨병 환자군에서는 연령의 증가와 유병기간이 혈액응고항진 및 용해의 장애에 큰 역할을 함을 알 수 있었으며, 총 당뇨병 환자군에 PIC와 HbA1c와 양의 상관관계를, BMI와 음의 상관관계를 보였으며 혈관 합병증이 없는 당뇨병 환자군에서만 TAT는 HbA1c와 양의 상관관계를 보였다. 따라서 당뇨병 환자에서 혈액응고 및 용해의 장애가 동반되어 있다고 볼 수 있으며, 혈장 TAT 및 PIC는 혈관합병증으로의 진행을 예측하는 지표로서 유용하리라 생각된다. 또 혈당조절정도와 상관성이 있으므로 혈당조절후에 추적검사를 시행하여 합병증의 예방이 가능한지 추후 연구가 필요하리라 생각된다. Background : Abnormality of coagulation and fibrinolystic system is known as a predisposing factor of vascular complication in diabetes. Although the pathogenesis is not well known, non-enzymatic glycation reaction and the increase in production of free radicals due to an increased oxidative stress may be linked to the hypercoagulibility and hypofibrinolytic activity. As indices of abnormality in coagulation and firinolysis in peripheral blood, plasma thrombin-antithrombin Ⅲ complex (TAT) and plasmin-α_2-plasmin inhibitor complex (PIC) were measured. The purpose of this study was to clarify whether hypercoagulability exists in diabetic patients with or without vascular complication. Methods : In our study, we measured plasma thrombin-antithrombin Ⅲ compelx (TAT) and plasmin-α_2-plasmin inhibit or complex (PIC) in 101 diabetic subjects and 20 controls. Comparing TAT and PIC levels in diabetic microvascular complication group, diabetic macrovascular complication group and controls, we examined correlation between risk factors associated with diabetic vascular complication. Results : 1. The group with diabetic vascular complication was older than group without complication. There was no significant difference in BMI, blood pressure, HbA_ic, blood sugar level, insulin, C-peptide, serum creatinine, total cholesterol, triglyceride, HDL-cholesterol, Lp (a) between two groups. The group with diabetic microvascular complication had longer duration of diabetes. 2. Concentration of TAT and PIC were 2.8±1.2 ng/ mL, 240.4±69.7 ng/ mL in controls and 9.5±22.6 ng/ mL, 472.2±258.7 ng/ mL in diabetic patients, respectively. TAT and PIC were significantly higher in diabetic patients than in control (p<0.001). But TAT/PIC ratio was no significant difference between two groups. 3. In diabetic patients, concentration of TAT and PIC and fibrinogen were respectively 4.1±2.4 ng/ mL, 362.2±272.0 ng/ mL, 322.7±102.4 mg/ dL in group without vascular complication and 5.3±4.1 ng/ mL, 529.5±258.7 ng/ mL, 374.9±106.2 mg/ dL in group with microvascular complication, which group had increase in PIC and Fibrinogen but no significance after correction of age. Concentration of TAT and PIC and Fibrinogen were 60.±4.9 ng/ mL, 507.4±321.6 ng/ mL, 427.1±194.7 mg/ dL in macrovascular complication, and 10.4±6.7 mg/ mL, 484.8±269.7 ng/ mL, 388.4±132.4 mg/ dL in combined vascular complication which group showed increase of TAT but also had no significant increase after correction of age. 4. In diabetic microvascular complication patients, group of high HbA_1c (>8%) (p=0.049) had significant high PIC concentration. In diabetic macrovascular complication patients, group of high HbA_1c (>8%) (p=0.042) had significant high total cholesterol concentration. 5. In all diabetic patients, PIC was positively correlated with fibrinogen and HbA_1c and negatively correlated BMI (r=0.47, 0.31, -0.25). Only in daibetic patients without angiopathy, TAT was positively correlated with HbA_1c (r=0.67). Conclusion : In this study, plasma TAT and PIC concentration significantly increased in diabetic patients compared with controls, and PIC was increased in group with microvascular complication, TAT were increased in group with combined micro macrovascular complication. However, there was no significance relationship existed when correctinf for age. PIC was correlated with HbA_1c. TAT was correlated with HbA_1c only in the group without angiopathy. Abnormality of coagulation and fibrinolysis were combined in diabetes, plasma TAT and PIC can be used as an index of vascular complication. Also we found the correlation with the degree of the blood glucose control. Therefore we need follow up study for the possibility of prevention of vascular complication after controlling the blood glucose to age-matched patients (J Kor Diaabetes Asso 25:354~363, 2001).

세기변조방사선치료의 선량분포 검증에 관한 연구

권수일,신동호,송재영,안진호 京機大學校 2006 論文集 Vol.50 No.-

Film dosimetry as a part of patient specific intensity modulated radiation therapy quality assurance (IMRT QA) has been accomplished to develop the new optimization method of film isocenter offset and to suggest the new quantitative criteria for film doimetry after optimization. Five patients with head and neck cancers were measured in IMRT dosimetry by film method. Optimization method with the local minimum was developed to adjust the film isocenter offset error, which is the biggest part of systemic errors. The mean ratios were 5.33% and 7.09% for 5mm and 1 mm resolution dose data optimization respectively.

시각장애 당뇨병 환자의 보행지도 전략

임안수 한국특수교육문제연구소 2001 특수교육저널 : 이론과 실천 Vol.2 No.1

현대 의학이 발전되고 있음에도 불구하고 당뇨병의 발생률은 증가되고 있으며, 당뇨병의 합병증으로 당뇨병 환자들의 삶의 질이 더욱 악화되고 있다. 특히 새로 실명하는 시각장애인의 12% 가 당뇨병 환자이고, 시각장애인이 된 당뇨병 환자의 가장 큰 문제 중의 하나가 보행이다. 보행교사는 당뇨병 환자에게 지팡이 보행이 적합한지 안내견 보행이 적당한가를 검사하는 것은 물론 당뇨병의 합병증에 따른 보행지도 전략을 세워 보행지도를 해야 한다. This article describes ocular and other complications of diabetes that affect orientation and mobility. Every year many clients with diabetes lose their sights by the causes of diabetic retinopathy, cataracts and glaucoma. Once they lose their sights it is necessary for them to receive independent living skills training as well as orientation and mobility training. Thus orientation and mobility instructor should have good knowledge and rich experiences to teach orientation and mobility skills to visually impaired persons with diabetes. They also should consider several important strategies. The orientation and mobility specialists help visually impaired clients with diabetes to regain psychological comfort when they are depressed with severe vision loss. If an insulin reaction is not properly treated, the visually impaired client can eventually lead to unconsciousness during mobility training. When orientation and mobility instructor trains the visually impaired persons with diabetes, interdisciplinary team should be formed in order to individualize orientation and mobility program. Finally, The orientation and mobility instructor recommends that the visually impaired persons with diabetes will take dog guide traning or long cane training after assessing those clients.

DNA 수준에서의 형별법 확립이 시급한 HLA-Cw

안수진,전태연,김태규,한훈 대한조혈모세포이식학회 1999 대한조혈모세포이식학회지 Vol.4 No.1

조혈모세포 이식시 HLA 대립유전자 형벌이 갖는 중요성은 주지의 사실이고, 최근에는 타인간 조혈모세포이식이 활발히 진행되고 있어 더욱 정확한 HLA 대립유전자 형벌이 요구되고 있는 실정이다. 특히 HLA-Cw 항원은 자연살해세포와 상호 작용할 뿐만 아니라, HLA -A,B 항원과 마찬가지로 항원성 펩타이드를 세포용해성 T 림프구에 제시하여 면역 반응에 관여하고, 한가지 항원만이 검출되는 비율이 약50%나 되므로, HLA-Cw 대립유전자 형별은 필수적이라 하겠다. 그러나 HLA-Cw 항원은 HLA-A,B 항원과 비교하여 10%정도가 세포 표면에 표현되므로, 동종항체 형성률이 낮아 35종류 이상의 대립유전자가 존재하여도, 8종류 (Cw1-8) 의 표현형에 대한 항체만이 사용되고 있는 실정이다. 본 연구에 의하면 한국인에 존재하나 혈청학적인 방법으로 검출할 수 없었던 대립유전자가 약 20%에 달하고, 50%이상의 출현빈도를 보이는 Cw3 대립 유전자는 서로 다른 아형을 갖고 있다. 다시 말해, 현재의 HLA-Cw 대립유전자 형별법은 위음성률이 높고 부정확하므로 면역세포인 조혈모세포의 이식성공률을 놓이기 위해서는 DNA 수준에서의 형별법이 시급히 요청된다. In hemopoietic stem cell transplantation(HPSCT), the identification of HLA allele is very important. Recently, the unrelated hemopoietic stem cell transplantation (UHPSCT) is briskly progressed, and more accurate identification of HLA allele is required. By means of conventional serology, HLA-Cw cannot be phenotyped precisely. And in approximately 50% of the case, only one HLA-Cw molecule can be determined, so the accurate identification is very indispensible in HLA-Cw alleles. Especially, HLA-Cw molecules are interacted with class I receptors expressed on natural kill cells. Like HLA-A and -B, HLA-Cw molecules can present the fragmented antigens to cytotoxic T lymphocytes, which can be participated in immune response Therefore, the typing of HLA-Cw allele is indispensable in immune response. But HLA-Cw molecules are expressed to a low molecules (10%) compared to HLA-A, -B antigens. Although over 35 types of HLA-Cw allele are present, only 8 types (Cw1-Cw8) of HLA-Cw antibody have been used because evaluated rates of alloantibody are low. In this study, with the ARMs-PCR method, we identify 18 HLA-Cw alleles and over 20% of HLA-Cw alleles have not been identified by serology in normal Koreans (n=241). The most frequent HLA-Cw allele is HLA-Cw*03 which consists of different subtypes. And among them two subtypes, Cw*0303 and Cw*0304, comprise more than 50%. In conclusion, the conventional serology method may not detect an additional allele or may determine the allele incorrectly. It is suggested that DNA-based method is an urgently required step to increase the success rate of hemopoietic stem cell transplantation.

게르만어 모음교체와 고대영어 강변화 동사

안수영 東新大學校 1999 論文集 Vol.11 No.-

Ablaut, a traditional feature of Indo-European tense formation, is a morphologically conditioned alternation which can occur in lexical, derivational, or inflectional morphemes. The most frequent alternations are /e, e-, o, o-/ and φ. The place where the Germanic reflex of ablaut is most obvious is in the strong verb paradigm. Old English is seen to have evolved the Germanic principle with such consistency that virtually all its primary verbs came to be arranged in rigid ablaut series. OE strong verbs have been classified into seven classes according to the vocalism of their four principal parts, i. e., the present, the preterit singular, the preterit plural and the past participle. Its criteria, however, are not purely synchronic but are essentially affected by historical consideration. In other words, the 'traditional classification' takes into consideration several sound changes such as breaking, pre-nasal raising, etc. which blurred original about patterns. Even though it is obvious that the criteria for classification which is relevant in pre-Germanic or Indo-European are no longer functional, the 'historicism' of historical linguistics should not be depreciated too much.

교근과 이하선 및 하악골에 발생한 횡문근 육종의 치험례

정수일,박재범,이계영,김도균,안재진 大韓顎顔面成形再建外科學會 1998 Maxillofacial Plastic Reconstructive Surgery Vol.20 No.1

外國語學習에 있어서의 二元論的 立場

安秀雄 釜山工業大學 1982 論文集 Vol.23 No.1

In the last twenty years, language acquisition theories were polarized with behaviorism and cognitivism at each end of the pole. Language teachers were so confused that they lost their sense of direction in the theory and methodology. In this paper the writer attemps to make clear this confusion with dualism. The writer's claim is that both are two intrinsic aspects of language, which are complementary to each other. It is regretful that Korean linguists are reluctant to acknowledge that this dualism solves the confusion. They tend to follow the currently popular theory like a fashion and can't get out of the dogma, failing to see the truth of the other side. Actually we can deduce this dualism from the fact that the development of the two theo-ries were cyclical. The cyclicity of the theories means that each of the theories has its own truth and can be combined to work as a synthesis, as Hegelian dialectic does : thesis-antithesis-synthesis. Another proof that both theories are right comes from the fact that Saussure's and Chom-sky's dichotomies reveal the truth of both. Saussure's Langue is a set of passively acquired habits, while Chomsky's competence is the perfect, abstract knowledge of the ideal speaker-hearer in a homogeneous speech community. So we can formulate a new dichotomy in language acquistion as following : It is not good that Korean linguists are too much inclined toward Chomskyan competence and underestimate or ignor the merits of the stucturalism. The writer asserts that to develop communicative competence, we must acknowledge tghe importance of the habitual aspect of language acquisition and that it is necessary to concentrate our study on habit-formation mechanism more thoroughly.

단일전력단으로 구성된 AC/DC 풀 브리지 컨버터에 관한 연구

안병무,권순도,김용,백수현 동국대학교 산업기술연구원 2000 산업기술논문집 Vol.11 No.1

본 논문에서는 단일단 AC/DC 폴브리지 토포로지에 적합한 고주파 소프트 스위칭 컨버터응용에 근거를 두고 제안한다. 제안된 컨버터는 입력전류를 불연속 모드로 동작시켜 역률을 개선하며 입력전류의 zero-crossing-point에서의 왜곡을 개선함으로써 고조파를 감소시켜 고역률, 영전압 스위칭, 저잡음과 고효율을 구현한다. 펄스폭 변조 제어방식은 각각의 스위칭과 정류손실을 저감하기 위해 사용되었다. 제안한 컨버터는 IGBT를 전력스위치로서 주로 사용되지는 고전압, 고전력 컨버터응용에 적용할 수 있으며, 실험을 통하여 1.5kW, 30kHz로 동작하는 컨버터를 구현하였다. A single stage AC/DC converter based on a full bridge topology suitable for high frequency soft switching converter applications is proposed. The power factor correction can be achieved by the discontinuous conduction mode(DCM) operation of the input current. The proposed converter gives the good power factor, low line current harmonics. The proposed converter has high power factor, zero voltage switching low noise and high efficency. A pulse width modulation control is employed to reduce the switching and rectification losses respectively. The proposed converter is attractive for high-voltage, high-power applications where IGBT's are predominantly used as the power switches. The principle of operation, features, and design are verified on a 1.5kW, 30kHz, IGBT based experimental circuit.

경량 고강도 Mg-Zn계합금의 조직과 내식성에 관한 연구

안효준,남태현,임수근,안인섭,허보영,김기원 慶尙大學校 1995 論文集 Vol.34 No.1

The purpose in this study is to develope of high strength and corrosion reistant Mg-Zn system alloys by controlling microstructures and by adding of Cu elements. To produce optimum tesile strength,it is imperative to obtain effective grain refinment during casting. Optimal condition for grain size refining effect was obtained at the minmum composotion of 2wt% Cu or 1.5wt%So addition to Mg-6wt%Zn alloy.Age hardening behavior was experimented at the optimal compositions of the Mg-6wt%Zn, Mg-6wt%Zn-2wt%Cu and Mg-6wt%Zn-1.5wt%Si. The hardeness increment due to age hardening was at the Mg-Zn-Si alloy system. The improvement of corrosion resistanece by the addition with Cu is related to and increasing Zn/Cu ration in the intermatallic particles in the Mg-Zn system.Further improvements obtained by T6 treatment are related to the properties of the Mg-Zn phase,whih acts as a corrosion barrier by precipitating along the grainboundaries.Filiform corrosion observed soon after pit initiation and cellular form of pit propagation later in chloride media are explained in terms of solid solution matrix structure and the properties of air formed oxide.The information obtained from the fundamental research work is utilized in suggesting ways of improving the corrosion resistance of commercial AZ91.