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      • MMA계 폴리머 시멘트 모르타르의 중성화에 대한 저항성

        조영철,형원길,김완기,소양섭 대한건축학회 2004 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.24 No.1(구조계)

        At present, the polymer-modified mortars are used as high-performance as well as multi-functional materials in the construction industry. The purpose of this study is to synthesize polymer to modify in cement mortars and make test samples to understand the resistance of carbonation. This paper deals with the effect of monomer ratio and polymer-cement ratios on the typical properties of polymer-modified mortars using Methylmethacrylate-Butyl Acrylate(MMA/BA) latexes synthesized through emulsion polymerization. From the results, we knew that the resistance for carbonation of polymer-modified mortars using Methylmethacrylate- Butyl Acrylate latexes is increased with an increase in the monomer ratio of MMA/BA and the polymer-cement ratio. the resistance for carbbaonation of polymer-modified mortars using Methylmethacrylate-Etyl Acrylate latexes is increased with an increase in the polymer-cement ratio. The most resistance for carbonation is MB8 and ME6 polymer-modified mortar.

      • 유화제 첨가량이 MMA/BA 합성 라텍스 폴리머 시멘트 모르타르의 특성에 미치는 영향

        형원길,조영철,장성주,김완기,소양섭 대한건축학회 2003 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.23 No.2

        The purpose of this study is to clarify the effect of the emulsifier ratio on properties of the polymer modified mortar using methylmethacrylate-buthyl acrylate(MMA/BA) latexes, and to obtain basic data necessary to develope appropriate latexes for cement modifiers. Polymer modified mortars using MMA/BA latexes are prepared with various monomer ratios, and tested for air content, flexural and compressive strengths. From the test results. we knew that the water-cement ratio is decreased with an increase in the amount of emulsifier. And the air content is increase with an increase in the polymer cement ratios. In general, the superior compressive and flexural strength of polymer-modified mortars using MMIA/GA latexes is obtained ant a bound MMA content of 80 percent and a emulsifier ratio of 6 percent.

      • 폴리에스테르 모르타르의 경화수축

        최낙운,김완기,조영국,소양섭 대한건축학회 2003 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.23 No.2

        The setting shrinkage behavior in polyester mortars with two-phase shrinkage reducing agent(SRA) was observed at the ambient temperature. Polyester mortars were prepared with various styrene contents and two-phase shrinkage reducing agent(SRA) contents. The length change and exotherm temperature of polyester mortars were continuously monitored by a noncontact-type automatic length change measuring apparatus and a thermocouple embedded respectively. Low-shrinkage or non-shrinkage of polyester mortars should be accomplished by adjusting styrene content and SRA content.

      • 백혈병 마우스 모델의 동종골수이식에서 활성화된 자연살해세포들의 보충이 이식편대백혈병효과와 이식편대숙주반응에 미치는 영향

        엄현석,한치화,박수정,김소연,정낙균,정대철,진종률,최일봉,양형모,서영훈,송현근,최인표,민우성,김춘추 대한조혈모세포이식학회 2001 대한조혈모세포이식학회지 Vol.6 No.1

        배경: 백혈병에서 동종골수이식 (allogeneic bone marrow transplantation)의 성공적 치료 효과를 얻기 위해서는 이식편대숙주반응 (graft-versus-host disease, GVHD) 발생의 극복과 재발의 방지가 중요한 과제이다. 골수를 역류원심성 세포분리 (counterflow centrifugal elutriation, CCE) 방법으로 분리하여 얻은 rotor off (R/O) 세포분획은 T 세포의 수는 적지만 조혈모세포들을 다량 포함하고 있어 동종골수이식에서 주조직적합복합체 (major histocompatibility complex, MHC) 차이를 극복할 수 있고, 이식편의 생착 성공과 GVHD 발생 예방에 효과적이다. 그러나 골수로부터 T 세포를 제거하면 백혈병세포를 공격하는 이식편대백혈병 (graft-versus-leukemia, GVL) 효과가 감소되기 때문에 백혈병 재발의 빈도가 높다. 자연살해세포 (natural killer cell, NK cell)의 보충 첨가는 동종골수이식 후 GVHD 발생을 줄이면서 충분한 GVL 효과를 얻을 수가 있다. 따라서 저자는 분리 후 IL-2로 활성화시킨 NK 세포들을 골수 R/O 세포분획과 함께 백혈병 마우스 모델에 동종이식함으로써 GVHD와 GVL에 미치는 효과를 관찰하였다. 방법: Balb/c (H-2^(d)) 마우스에서 유래된 A20 (murine B-lymphoma/leukemia cell line, H-2^(d)) 백혈병 세포를 이식 2 일 전에 Balb/c 마우스에 주입하고, 치사량의 전신 방사선을 조사한 직후에 Balb/c 또는 C57BL/6 (H-2^(b)) 마우스의 골수 R/O 세포분획을 꼬리정맥을 통하여 주입하였다. 이들은 모두 이식 후 6-8 주 이내에 사망하였다. 동종이식의 대조군 (n=9)에는 1 × 10^(7)의 R/O 세포분획만을 주입하였고, 실험군 (n=9)에는 C57BL/6 마우스의 비장세포들로부터 단클론항체들을 이용한 negative selection방법으로 분리한 후 IL-2로 활성화된 5 × 10^(5)의 NK 세포분획을 1 × 10^(7)의 R/O 세포분획과 함께 주입하였다. GVL 효과의 판정은 이식 후 14 일과 28 일 째 되는 날 마우스에서 골수, 비장, 간 등을 얻어 백혈병 세포들의 침윤을 조직학적으로 관찰하였으며, GVHD의 정도는 육안적 관찰법으로 평가하였다. 결과: R/O 세포분획만을 이식한 대조군의 골수, 비장, 그리고 간 조직에서는 A20 백혈병 세포의 침윤이 각각 89% (8/9), 78% (7/9)와 22% (2/9)에서 관찰되었고, R/O 세포분획과 NK 세포분획을 함께 이식한 실험군에서는 비장과 간을 제외한 골수에서만 89% (8/9)에서 A20 백혈병 세포의 침윤이 관찰되어 두 군 사이에 장기별 분포의 차이를 볼 수 있었다 (P= 0.0001). 한편 GVHD는 두 군 모두에서 경하게 나타나서 유의한 차이는 없었다. 또한 생착 부전으로 사망한 마우스는 없었다. 결론: CCE를 이용하여 T 세포를 제거한 동종골수이식에서 NK 세포의 보충은 GVHD의 악화는 일으키지 않으면서, 백혈병의 진행을 억제하는 GVL 효과를 얻을 수 있었다. Background: Allogeneic bone marrow transplantation (BMT) with T cell-depleted marrow accompanies engraftment failure and relapse of leukemia by a loss of the graft-versus-leukemia (GVL) effect frequently, while it can prevent GVHD. Supplement of NK cells could prevent GVHD and enhance GVL effect in several murine allogeneis BMT models Roter off (R/O) cell fraction obtained by counterflow centriation elutriatio (CCO) contains small number of T cells and many hematopoietic stem cells. The aim of this study was to determine the effect of R/O cell fraction supplemented with IL-2 activated NK cells on GVL and GVHD within the leukemic mouse BMT model. Methods: Inoculation of A20 (H-2d, murine B-lymphoma/leukemia, Balb/c origin) cells into Balb/c mice via the tail vein 2 days prior to lethal total body irradiation (TBI) and infusion of the Balb/c BM or C57BL/6 (H-2b) R/O fraction were performed. It resulted in 100% mortality within 6 to 8 weeks. The irradiated mice in the control group were injected with 1 × 107 R/O cell fraction alone (n=9) and in the experimental group mice were injected with 1 × 107 R/O cell fraction plus 5 × 105 negatively selected IL-2 activated NK cell fractions of the spleens via the tail vein (n=9). On day 14 and 28 after BMT, the bone marrows, spleens, and livers of mice were harvested for histopathologic analysis of the infiltrations of leukemic cells. We then evaluated the GVHD within the mice. Results: A histopathologic study of the recipients receiving R/O fraction alone showed infiltration of leukemic cells, 89% (8/9) in bone marrows, 78% (7/8) in spleens, and 22% (2/9) in livers. The experimental group of mice showed only the infiltration of leukemic cells 89% (8/9) in bone marrows, not in spleens and livers. There were the organ differences of the leukemic cells infiltrations between the two groups (P=0.0001). There were no obvious differences in the GVHD scores between these two groups, and severe GVHD was not observed. There was no engraftment failure among groups. Conclusion: Thus, our findings suggest that R/O cell fraction obtained by CCE and supplemented with NK cells can promote GVL effect without mediating clinically overt GVHD in allogeneic BMT of mouse leukemia.

      • SCIESCOPUSKCI등재

        Preparations of Carbon Fibers from Precursor Pitches Synthesized with Coal Tar or Petroleum Residue Oil

        Yang, Kap-Seung,Park, Young-Ok,Kim, Yong-Min,Park, Sang-Hee,Yang, Cheol-Min,Kim, Yong-Joong,Soh, Soon-Young The Korean Fiber Society 2000 Fibers and polymers Vol.1 No.2

        Pitch precursors were synthesized from coal tar(CT) and pyrolysis fuel oil(PFO, petroleum residue oil) at relatively low temperature of $250^{\circ}$, in the presence of horontrifluorideidiethyletherate complex(BFDE) as a catalyst and nitrobenzene(NB) as a co-catalyst. The softening point, nitrogen content and carbon yield increased with an increase of concentration of NB. The pitch precursors with good spinnability were prepared by removing the volatile components through $N_2$ blowing. The precursor pitches were spun through a circular nozzle, stabilized at $310^{\circ}$ and finally carbonized at $1000^{\circ}$. The optically anisotropic structure formed at the absence of NB was changed into isotropic structure, showing a decrease in size of the flow domain. The hollow carbon fiber could be prepared in the process of stabilization. The results proposed that the morphology of carbon materials could be controlled by changing the concentration of catalyst and/or co-catalyst and/or stabilization condition that affect on the mobility of molecules during carbonization.

      • KCI등재후보

        후복막강에 발생한 고립성 악성 신경초종 1 예

        김영자,김영배,홍영규,양윤모,최규식,최인준,이경식,소의영,황영남,조승운 대한내과학회 1988 대한내과학회지 Vol.35 No.5

        Solitary malignant schwannoma is a rare primary nerve sheath tumor and has been reported to occur in almost any anatomic location, most commonly in the extremities, but rarely in the retroperitoneum. The typical clinical presentation is that of a painless, slowly enlarging soft tissue mass, occasionally associated with a peripheral neuropathy. A high percentage of the patients experience local recurrence even after radical surgical therapy and the results of treatment with radiation and chemotherapy have also been disappointing. This paper presents a case of solitary malignant schwannoma occuring in the retroperitoneal cavity with a brief review of the pertinent literature. A 71 year-old male visited our medical hospital with the symptom of a progressive growing painless mass in the left upper abdomen of 2 months duration. Abdominal ultrasonography and CT scan highly suggested a pancreatic tail carcinoma or primary retroperitoneal tumor. Radical surgical resection was performed successfully and the histological findings were compatible with malignant schwannoma.

      • The Diagnostic Value of a Digital Rectal Examination Compared With High-Resolution Anorectal Manometry in Patients With Chronic Constipation and Fecal Incontinence :

        Soh, Jae Seung,Lee, Hyo Jeong,Jung, Kee Wook,Yoon, In Ja,Koo, Hyun Sook,Seo, So Young,Lee, Seohyun,Bae, Jung Ho,Lee, Ho-Su,Park, Sang Hyoung,Yang, Dong-Hoon,Kim, Kyung-Jo,Ye, Byong Duk,Byeon, Jeong-Si American College of Gastroenterology 2015 The American journal of gastroenterology Vol.110 No.8

        <P>Digital rectal examination (DRE) is a simple clinical method to diagnose anorectal disorders. High-resolution antorectal manometry (HRAM) based on a spatiotemporal plot is expected to promote improved diagnostic accuracy. However, there are no reports comparing the effectiveness of DRE and HRAM. The aim of our study was therefore to evaluate the diagnostic value of DRE compared with HRAM.</P>

      • 식물 생장물질이 담배(Nicotiana glauca GRAH) 배양세포의 염색체 이상에 미치는 영향

        양우영,소웅영 全北大學校 基礎科學硏究所 1994 基礎科學 Vol.16 No.-

        식물 생장물질이 염색체 이상에 미치는 영향을 밝히고자 담배 캘러스를 여러 식물 생장물질이 다양한 농도로 첨가된 MS 배지에서 4주 간격으로 4-5회 계대배양하면서 염색체 이상을 조사한 결과, 염색체 이상은 NAA첨가 조건에서 가장 많았으며, 2,4-D 단독 첨가조건에서 농도가 증가할수록 염색체 이상도 증가하였다. 이와 같은 염색체 이상의 증가 원인이 식물 생장물질에 의해 직접적 혹은 간접적으로 영향을 받는지를 규명하고자 캘러스 생장률과 염색체 이상률을 비교한 결과, 2,4-D 단독 첨가 조건을 제외한 여러 식물 생장물질에 있어서 염색체의 이상의 증가는 캘러스 생장율이 증가함에 따라 염색체 이상도 증가하는 경향을 나타내었다.

      • A Simple and Rapid Gene Amplification from Arabidopsis Leaves Using AnyDirect System

        Yang, Young-Geun,Kim, Jong-Yeol,Soh, Moon-Soo,Kim, Doo-Sik Korean Society for Biochemistry and Molecular Biol 2007 Journal of biochemistry and molecular biology Vol.40 No.3

        Polymerase chain reaction (PCR) is a powerful technique in molecular biology and is widely used in various fields. By amplifying DNA fragments, PCR has facilitated gene cloning procedures, as well as molecular genotyping. However, the extraction of DNA from samples often acts as a limiting step of these reactions. In particular, the extraction of PCR-compatible genomic DNA from higher plants requires complicated processes and tedious work because plant cells have rigid cell walls and contain various endogenous PCR inhibitors, including polyphenolic compounds. We recently developed a novel solution, referred to as AnyDirect, which can amplify target DNA fragments directly from whole blood without the need for DNA extraction. Here, we developed a simple lysis system that could produce an appropriate template for direct PCR with AnyDirect PCR buffer, making possible the direct amplification of DNA fragments from plant leaves. Thus, our experimental procedure provides a simple, convenient, non-hazardous, inexpensive, and rapid process for the amplification of DNA from plant tissue.

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