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      • KCI등재

        물오리나무와 자작나무의 뿌리 및 줄기에 있어서 이기목부(二期木部)의 비교해부학적 연구

        소웅영,한경식 한국임학회 1985 한국산림과학회지 Vol.68 No.1

        물오리나무와 자작나무의 뿌리 및 줄기에 있어서 二期木部의 解剖學的 特徵과 細胞의 크기에 對하여 硏究하였다. 2種의 뿌리와 줄기에서 나타난 解剖學的 差異는 다음과 같다. 1) 穿孔板上의 橫帶數는 줄기에 比해 뿌리에서 더 많았고 이에 상응하여 穿孔板의 角度는 뿌리에서 더 큰 것으로 나타났다. 2) 導管 및 纖維의 直徑은 줄기에 比해 뿌리에서 훨씬 더 넓었다. 3) 導管要素의 길이는 뿌리에 比해 줄기에서 더 긴 반면 纖維의 길이는 뿌리에서 더 길었다. 4) 放射組織은 줄기에 比해 뿌리에서 훨씬 더 發達된 樣相을 보인다. 5) 뿌리에서 年輪은 比較的 뚜렷하였다.

      • KCI등재

        당근 세포배양으로부터 체세포배 발생에 미치는 아스콜빈산의 효과

        소웅영,김이엽,조덕이 한국식물생명공학회 1999 식물생명공학회지 Vol.26 No.3

        당근의 배양세포로부터 체세포배의 발생과정에 미치는 아스콜빈산 및 dehydroascorbic acid의 영향을 밝히기 위하여 본 실험을 시도하였다. 비배발생세포의 배양에 처리된 아스콜빈산은 세포증식을 촉진시켰을 뿐인데 dehydroascorbic acid는 세포증식을 억제시키면서 배발생세포로 전환시킨 효과가 있었다. 배발생세포의 배양에 처리된 아스콜빈산은 체세포배 발생을 억제시켰지만 dehydroascorbic acid는 체세포배발생을 촉진시켰다. 그러나 이와 같은 발생촉진은 구상배에서 중단되므로 성숙에는 오히려 저해적이었다. 이상의 결과로부터 당근의 캘러스배양에 dehydroascorbic acid를 처리하여 빠른 시일내에 배발생캘러스를 확보한 다음 dehydroascorbic acid 첨가 배발생 배지에서 초기배발생기간 배양 후 MS 기본배지로 옮겨 배양하면 고빈도의 체세포배생산 실험계가 확립될 것으로 판단된다. This study was conducted to elucidate the effects of ascorbic acid and dehydroascorbic acid on somatic embryogenesis from the cultured cells of carrot. Ascorbic acid in culture medium merely stimulated the proliferation of non-embryogenic cells but dehydroascorbic acid in medium induced embryogenic cells from non-embryogenic cells accompanying the inhibition of cell proliferation. Ascorbic acid in medium inhibited somatic embryogenesis from embryogenic cells while dehydroascorbic acid in medium enhanced somatic embryogenesis from the cells as well as non-embryogenic cells. This enhancement was limited to globular embryos and the maturation to cotyledonary embryos was inhibited by dehydroascorbic acid treatment. From the above results it is suggested that carrot callus cultures on medium containing dehydroascorbic acid could quickly induce embryogenic cells. In addition after brief culture of embryogenic cells on development medium containing dehydroascorbic there by acid the subculture of the cells to MS basal medium resulted in the high frequency production of somatic embryos.

      • SCOPUSKCI등재

        韓國産 木本植物에 대한 系統分類學的 硏究 : 녹나무科 植物뿌리의 木部解剖 Anatomy of Lauraceous Root Wood

        蘇雄永,朴相珍 한국식물학회 1984 Journal of Plant Biology Vol.27 No.3

        Anatomical characters of the secondary xylem in roots of Lauraceae, including 6 genera and 13 species grown in Korea, were studied systematically. Lauraceous members studied anatomically possess characters of the secondary xylem that are in the main more specialized than those found in the primitive angiosperm families. The specialized characters are as follows; ⑴ mostly solitary pores with some radially multiples, ⑵ mostly simple perforation plates and partially scalariform perforation plates in all species, ⑶ slightly oblique end wall of vessel element, ⑷ predominantly alternate intervascular pitting, ⑸ paratracheal axial parenchyma in all species, ⑹ heterogeneous Ⅱ or Ⅲ vascular rays. It is considered that the series of specialization in this family from the perforation plates and angle of end wall to the vessel axis is as follows; Machilus→Neolitsea→Lindera→Cinammomum→Iozoste→Litsea.

      • SCOPUSKCI등재

        數種의 韓國産 자작나무科 植物에 있어서 뿌리와 줄기의 二期木部의 比較解剖

        蘇雄永,韓京植 한국식물학회 1985 Journal of Plant Biology Vol.28 No.2

        A comparative anatomy between the secondary xylem in the root and stem of Korean Betulaceae, including 5 genera and 6 species, was carried out in this study. Anatomical characteristics of the secondary xylem in the root and stem are as follows: Diameter of vessel and fiber is wider in the root than the stem, while the number of vessel and fiber per unit area is fewer in the root than the stem. The length of vessel element is longer in the stem than the root, whereas length of the fiber is longer in the root than the stem. Number of bar in the perforation plate is more in the stem than the root, and the angle of perforation plate is broader in the root than the stem. Number of ray per unit area is more in the root than the stem.

      • SCOPUSKCI등재

        韓國産 木本植物에 對한 系統分類學的 硏究 : 목련科, 붓순나무科 및 오미자科의 比較木部解剖 A Comparative Wood Anatomy of Magnoliaceae, Winteraceae and Schizandraceae

        蘇雄永,朴相珍 한국식물학회 1985 Journal of Plant Biology Vol.28 No.4

        Anatomical characters of secondary xylem in the trunk of Magnoliaceae, Winteraceae and Schizandraceae, including six genera and eleven species grown naturally in Korea, were studied to elucidate the relationship between genera or families. It is considered that among these families specialization in the perforation plate, the angle of end wall to the vessel axis, and diameter of vessel element, is in the order of Winteracae, Magnoliaceae, and then Schizandraceae. In Winteraceae, vessel elements have wholly scalariform perforation plates with very numerous bars. Among Magnoliaceae the perforation plates are scalariform with very numerous bars in Michelia, with few bars in Liriodendron, and with few bars or simple in Magnolia. In Schizandraceae, Schizandra shows scalariform perforation plates with few bars or simple perforation plates, and Kadsura shows almost simple ones.

      • SCOPUSKCI등재

        당근의 배양세포로부터 형성된 체세포배의 다자엽 구조

        蘇雄永,趙德以,李銀慶 한국식물학회 1996 Journal of Plant Biology Vol.39 No.1

        배발생 캘러스의 선발은 1.0 ㎎/L 2,4-D를 첨가한 MS 기본배지에서 배양한 당근 유식물의 배축절편으로부터 유도된 캘러스에서 이뤄졌다. 체세포배 발생을 유도하기 위해서 배발생 캘러스로부터 얻어진 세포괴는 2,4-D를 제거한 MS 배지에 옮겼다. 세포괴를 1.0 ㎎/L 2,4-D 첨가배지에서 1주간 그리고 2,4-D 제거 배지에서 2주간 배양한 후에 2자엽배의 발생은 63%에 그쳤고 나머지는 1자엽 5%, 3자엽 21%, 4자엽 6%, 5자엽 5%, 6자엽 0.2% 및 나팔형 자엽 1%의 발생빈도를 나타냈다. 체세포배의 발아율은 다자엽 체세포배에서 보다 2자엽 배에서 높았다. 나팔형 자엽의 체세포배는 경엽부 발생없이 자엽과 뿌리가 다소 신장 확대되는데 그치는 등 정상적으로 발아되지 못했다. 해부학적인 관찰에서 체세포배의 뿌리의 환상 전형성층은 배축의 중간부위에서 분지되기 시작하고 자엽절을 거쳐서 자엽으로 이어지는데 배축에서 전형성층속은 자엽수와 같은 수였다. 1자엽 체세포배는 다자엽 체세포배의 각각의 자엽보다 크고 전형성층과 함께 말굽형 자엽을 가지고 있었다. 따라서 체세포배의 자엽구조는 전형성층 배열상태와 밀접한 관련이 있다는 것을 확인할 수 있었다. Embryogenic callus was selected from callus induced from hypocotyl segment cultures of Daucus carota seedlings on MS basal medium supplemented with 1.0 ㎎/L 2,4-D. Cell clumps prepared from the embryogenic callus were transferred to MS medium without 2,4-D for somatic embryo development. Cotyledonary abnormalities were frequently observed on somatic embryos developed after the incubation of cell clumps in MS basal medium with 1.0 ㎎/L 2,4-D for one week and then subculture in the same medium but without 2,4-D for two weeks. The percentage of abnormalities was as follows: 5% one cotyledon, 21% three cotyledons, 6% four cotyledons, 5% five cotyledons, 0.2% six cotyledons and 1% trumpet-like cotyledons. On the other hand, the normal somatic embryo with two cotyledons appeared at 63%. The germination rate of somatic embryos was higher in two cotyledon somatic embryos than in multicotyledonary embryos. Trumpet-like somatic embryos did not germinate normally showing limited elongation and enlargement of roots and cotyledons without shoot development. From anatomical examination circula procambium in the root of somatic embryo began to branch around the middle regions of the hypocotyl which extended into the cotyledons through the cotyledonary nodes and the number of branched procambial strands in hypocotyl was equal to the number of cotyledons. Monocotyledonous somatic embryo always had larger cotyledon than that of somatic embryos with multicotyledons and had horseshoe-like cotyledons where the procambium was of the same structure.

      • SCOPUSKCI등재

        韓國産 木本植物에 對한 系統分類學的 硏究 : 차나무科의 木材比較解剖 A comparative wood anatomy of Theaceae

        蘇雄永,宣炳崙 한국식물학회 1986 Journal of Plant Biology Vol.29 No.4

        Anatomical characters of the trunk woods of Theaceae, including six genera and seven species collected in Korea, were studied to elucidate the relationship among the genera of the family. Among three genera in Ternstoemieae, Eurya is the least specialized, and Cleyera is less specialized than Ternstroemia in respect to the specialization of the perforation plate and the ray. In Theoieae consisting of three genera, the most primitive one is Thea, and Camellia is more primitive than Stewartia in the trive, from the view of the specialization of the bar arrangement in scalariform perforation plate and the angle of end wall to the vessel axis. The specialization of the perforation plate of vessel element suggests that Theoieae is more advanced group than Ternstroemieae.

      • SCOPUSKCI등재

        고사리삼 지하경의 유관속 분열조직 미세구조

        蘇雄永,金永順 한국식물학회 1993 Journal of Plant Biology Vol.36 No.4

        To elucidate the origin of secondary growth in the rhizome of B. ternatum, the developmental changes of vascular cambium was observed in ultrastructural features. The vascular cambium was gradually differentiated from procambium as in seed plants, but the cambial activity did not persist very long so that the cambial cells became a dormant state like fossil cryptogams. Dense cytoplasm of procambial cells became progressively sparse during the growth, and the tiny vesicles were fused to form numerous small vacuoles and then a few large vacuoles. These gradual changes and the occurrence of storage materials which was associated with the developmental stages might support the progressive differentiation of the cambial cells. In addition, the cessation of cambial activity could be indicated by the facts that late vascular cambial cells accumulate large lipid bodies and show very small peripheral cytoplasm and unlikely thickened cell wall, compared to other meristematic cells. Therefore, the vascular cambium showed the characteristics of both seed plants and fossil cryptogams from the view poiint of cambial ontogeny and activity.

      • SCOPUSKCI등재

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