물리․화학적 처리에 의한 요구르트 오염균의 생육 억제효과

선우찬(Chan Sunwoo),이소영(So-Young Lee),윤소영(So-Young Yoon),정지연(Ji-Yeon Jung),김꽃봉우리(Koth-Bong-Woo-Ri Kim),이청조(Chung-Jo Lee),곽지희(Ji-Hee Kwak),김민지(Min-Ji Kim),김동현(Dong-Hyun Kim),정슬아(Seul-A Jung),김현지(Hyun-Jee 한국식품영양과학회 2011 한국식품영양과학회지 Vol.40 No.12

물리?화학적 처리에 의한 요구르트 오염균의 생육억제 효과를 알아보기 위해, 요구르트의 주요 오염균을 분리?동정하고, 열, pH, 전해수, 오존가스, microwave 처리 및 감마선을 조사하여 오염균주에 대한 사멸효과를 알아보았다. 오염된 요구르트로부터 분리한 효모의 지방산 조성 분석과 API(Analytic Profile Index) kit 분석을 실시한 결과, Hanseniaspora uvarum으로 동정되었으며 잠정적으로 Hanseniaspora uvarum Y1으로 명명하였다. H. uvarum Y1의 열 및 pH 처리에 의한 생육억제 효과를 측정한 결과, 70℃ 및 80℃에서 15분 가열처리로 균이 사멸되었으며, pH 처리 시 pH 2, 3 및 9에서 생육이 다소 억제되었으며, pH 1 및 10에서 완전히 억제되었다. 전해수 처리의 경우, clear zone이 5 mm 이상으로 H. uvarum Y1이 전해수에 높은 감수성을 가지는 것으로 나타났다. 오존가스 처리에 의한 H. uvarum Y1의 사멸효과를 측정한 결과, 102 CFU의 균은 10분, 103 CFU의 균은 20분 처리 시 모두 사멸한 것으로 나타났으며, microwave 처리의 경우, 106 CFU 가량의 균이 1분 처리 시 모두 사멸되었다. 방사선 조사의 경우, 균수를 90% 이상 감소시키는데 필요한 조사선량이 20 kGy 이상으로 H. uvarum Y1은 감마선에 저항성이 있는 균임을 알 수 있었다. 이상의 결과를 종합해볼 때 열, pH, 전해수, 오존가스, microwave 처리를 통해 요구르트 오염균주인 H. uvarum Y1의 생육을 효과적으로 억제할 수 있을 것으로 생각된다. This study was conducted to investigate the cause of microbiological contamination in yogurt and evaluate the effect of physicochemical treatment on the growth inhibition of Hanseniaspora uvarum isolated from yogurt. The yeast strain Hanseniaspora uvarum Y1 was subjected to heat and pH treatments. H. uvarum Y1 was killed at 70oC and 80oC after 15 min and survived in a wide pH range from pH 2 to 9. However, it did not survive under pH 1 and over pH 10. In a disk diffusion susceptibility test on H. uvarum Y1, a clear zone (5 mm) of growth inhibition was observed upon treatment with electrolyzed water. The effect of ozone gas on the growth of H. uvarum Y1 was evaluated by viable cell count. Initial cell numbers of 102 and 103 CFU/mL of H. uvarum Y1 were completely killed by treatment for 10 and 30 min, respectively. H. uvarum Y1 was also sterilized by microwave treatment for 1 min. When treated with gamma-irradiation, the rate of killing of H. uvarum Y1 was proportional to the irradiation dose. and complete killing occurred at a dose of 50 kGy.

물리ㆍ화학적 처리에 의한 멸균 초콜릿 우유 오염균의 생육억제 효과

최문경(Moon-Kyoung Choi),윤소영(So-Young Yoon),이소영(So-Young Lee),김꽃봉우리(Koth-Bong-Woo-Ri Kim),이청조(Chung-Jo Lee),정지연(Ji-Yeon Jung),곽지희(Ji-Hee Kwak),김민지(Min-Ji Kim),김동현(Dong-Hyun Kim),선우찬(Chan Sunwoo),이주운(Ju-W 한국식품영양과학회 2011 한국식품영양과학회지 Vol.40 No.8

멸균 초콜릿 우유로부터 분리한 내열성 균주에 대해 열, pH, 전해수, 오존처리, microwave 및 감마선 처리를 하여 균주의 사멸효과에 대해 알아보았다. 균주의 지방산 분석과 API kit를 통하여 균주를 동정한 결과, Bacillus lentus로 동정되었으며, 잠정적으로 Bacillus lentus M1으로 명명하였다. B. lentus M1에 110℃, 15분간 열처리하였을 경우 생육이 억제되었으며, pH 처리 시 pH 5 이하, 10 이상에서 생육이 억제된 것으로 나타났다. B. lentus M1에 대한 전해수의 항균활성을 paper disc법으로 측정한 결과, 높은 생육억제를 보였으며, 오존 처리의 경우 초기 균수가 10² CFU가량의 균을 10분 동안, 10³ CFU가량의 균을 30분 동안 처리 시 균의 생육이 억제되는 것으로 나타났다. Microwave를 1분간 처리 시 B. lentus M1이 모두 사멸한 것으로 나타났다. 감마선 조사의 경우, 1 kGy 조사 시 생균수가 1.61×10³ CFU로 초기 균수에 비해 4 log cycle 가량 균수가 감소하였으며 7 kGy에서 완전히 사멸하였다. 이상의 결과를 통해 열, pH, 전해수, 오존 처리 및 방사선 처리 방법이 멸균 초콜릿 우유의 생존 오염균인 B. lentus M1을 효과적으로 사멸시킬 수 있을 것으로 사료된다. This study was conducted to investigate the cause of microbiological contaminants in aseptic chocolate milk and evaluate the effect of a physicochemical treatment on the growth inhibition of isolated bacterial strains. The bacterium isolated from aseptic chocolate milk was identified as Bacillus lentus and was named B. lentus M1. In the heat and pH treatment, the growth of B. lentus was inhibited at 110℃ for >15 min and at pH’s <5 and >10. An electrolyzed water treatment against B. lentus M1, revealed 5 ㎜ growth past the inhibition zone. The effect of ozone gas on B. lentus M1 growth was evaluated using viable cell counts. When the initial number of B. lentus M1 was 10² and 10³ CFU, the bacteria were completely suppressed by ozone gas treatment for 10 and 30 min, respectively. In a microwave treatment, B. lentus M1 was sterilized following microwave treatment for 1 min. As the result of γ-irradiation against B. lentus M1, numbers decreased as the γ-irradiation dosage increased. These results show the growth inhibition effects against contaminants in aseptic chocolate milk using physicochemical treatments.

A case of ectopic opening of common bile duct in duodenal bulb

( Ju Ri Kim ),( Hyun Cheol Koo ),( Ji Woong Jang ),( So Hee Chung ),( Min Kyu Kang ),( Jin Young Kim ),( Dong-kyu Lee ),( Jun Hyung Park ) 대한내과학회 2015 대한내과학회 추계학술대회 Vol.2015 No.1

Ectopic opening of common bile duct (CBD) in duodenal bulb is very rare, and it can be missed at endoscopy because of small size of the orifice that lacks typical feature of major duodenal papilla and duodenal deformity that makes endoscopic approach difficult. However, it should not be overlookedbecause of critical complications such as recurrent duodenal ulcer or cholangitis. Here we report one case of ectopic opening of CBD in duodenal bulb which was managed with endoscopic retrograde biliary drainage (ERBD) through endoscopic retrograde cholangiopancreatography (ERCP). The patient visited us for fever and abdominal pain. There were some significant findings in studies, which included stricture of duodenal bulb with ulcer and one loose slit-like opening in duodenoscopy, in addition to the tapered distal CBD with dilated proximal part in abdominal CT and ERCP. He treated with two times of ERBD insertion which improved duodenal ulcer and cholangitis, and discharged without any biliary related symptom. The common feature of ectopic opening of CBD isknown to be the absence of normal sphincteric structure. Since it allows free movement of bile and duodenal contents between CBD and duodenal bulb without sphincteric control, this disease frequently accompanies recurrent duodenal ulcer and cholangitis that can cause irreversible deformity. Another is the tapered distal CBD (so-called “hook shaped CBD” in several reports) with dilated proximal part in imaging studies. This is assumed to contribute to bile stasis that promotes bacterial growth or lithogeniccharacteristics of bile, which can lead to biliary disease. These complications can be managed with conservative way, but sometimes need surgical procedure, then, these situations need further accurate diagnosis because of risk for accidental biliary tract injury during surgery due to abnormal location of bile duct orifice. In conclusion, if we encounter recurrent duodenal ulcer or cholangitis in the field, we have to consider the possibility of ectopic opening of CBD.

5종의 치주질환균에 대한 프로폴리스의 항균효과

이은지,김소민,박소민,박복리,홍성익,김현정 순천향대학교 기초과학연구소 2022 순천향자연과학연구 논문집 Vol.28 No.1,2

Since the oral cavity is directly connected to the outside, it is an important site for microbial invasion, and since it is an environment that is nutritionally and physiologically easy to multiply bacteria, many bacteria always form bacteriological flora in the oral cavity. And propolis is a natural treatment used in many health food stores in various forms for local treatment. This study analyzes the changes in the activity of five oral microorganisms before and after consumption of propolis for various age groups to find out how propolis, which shows the efficacy of anti-inflammatory, antibacterial, and immune sensitization, affects oral microorganisms. Five types of bacteria possessed by periodontal disease patients were specified and digitized targeting the public person, and then before and after propolis intake was compared, and the levels of periodontal disease bacteria in the intake group and the non-consumption group were compared. As a result of comparing the patterns before ingestion (0 week) and after ingestion (3 week) in the oral bacteria analysis, it was confirmed that the bacteria decreased overall except for the five populations in the propolis intake group. In contrast, in the comparative group that did not intake propolis, the bacteria of five periodontal disease increased except for the four populations. What can be seen from this result is that propolis is effective in reducing bacteria in five types of periodontal disease. In order to use propolis as a food for preventing periodontal disease and to get a better effect, further research is needed to find out the mechanism of antibacterial action in propolis compounds.

임부의 연령에 따른 보완대체요법에 관한 인식 태도 경험 비교 연구

김연진,김지연,노경민,신혜리,유예지,이지민,장희조,정아인,최소정,강숙정,이향연,민혜영 이화여자대학교 간호과학대학 2016 이화간호학회지 Vol.- No.50

Purpose: This study aims to test if the age of pregnant women affects the perception, experience, and attitude towards complementary alternative therapy (CAT), by investigating the perception, experience, and attitude of pregnant women under the age of 35, and above 35 (Advanced Maternal Age). The study will 1) find the general characteristics of pregnant women under and over the age of 35 2) compare the perception, behavior, and experience of pregnant women under and over the age of 35, and 3) find the comparison of the perception, behavior, and experience according to the general characteristics of pregnant women under and over the age of 35. Method: The subjects of this study are composed of 44 women under the age of 35 and 44 women above the age of 35, totaling 88 pregnant women visiting midwiferies, obstetrics and gynecology, and baby fairs in Seoul and Gyeong-Gi area. Data for this study was collected from November 28, 2015 to January 6, 2016 for 40 days. The collected data analyzed mean, standard deviation, t test, ANOVA, and chi-square by using SPSS 23.0. Result Results show that there is a significant difference in the perception, attitude, and experience of the two-sample groups in the use of CAT. The question of “what is most necessary when applying CAT in a hospital clinical environment” for the perception criteria came out with the results of t=9.980, p<.05. For the attitude, results showed that the answers of pregnant women under the age of 35 displayed a more positive attitude towards CAT. (t=2.421, p<.05) Lastly, in the experience section, pregnant women under the age of 35 showed more CAT use than the other sample group. Conclusion Results have shown that there is a difference in the perception, behavior, and experience between the two sample groups. Therefore further research on the efficacy and the adverse effects of CAT needs to be done.

A 38-Year-Old Woman With Bilateral Cystic Lesions in Both Lower Lung Lobes

Kim, So Ri,Lee, Min Hee,Lee, Kyung Ae,Park, Seoung Ju,Min, Kyung Hoon,Han, Hyo Jin,Chung, Chi Ryang,Choi, Kyoung Hwa,Lee, Heung Bum,Jin, Gong Yong,Lee, Yong Chul Elsevier 2011 Chest Vol.140 No.2

제주도 구좌지역 해상풍력단지 예정지역의 물새류현황조사

김소리나(So-Ri-Na Kim),김병수(Byoung-Soo Kim),장민호(Min-ho Chang),경남호(Nam-ho Kyong),오홍식(Hong-Shik Oh) 한국조류학회II 2007 한국조류학회지 Vol.14 No.2

2006년 3월부터 2007년 2월까지 제주도 구좌지역의 해상풍력단지 예정지역의 물새류현황을 조사하였다. 조사결과 총 58종 16,786개체가 관찰되었으며, 종 다양도는 1.735로 나타났다. 우점 종은 재갈매기(Larus argentatus) 51.36%, 괭이갈매기(Larus crassirostris) 21.60%, 가마우지(Phalacrocorax carbo) 5.53%, 민물도요(Calidris alpine) 4.46%, 슴새(Calonectris leucomelas) 3.74% 등의 순으로 나타났다. 월별 관찰종수는 4월이 36종으로 가장 많았고 6월이 8종으로 가장 적었으며, 개체수는 2월이 7,972개체로 가장 많았고 6월이 33개체로 가장 적었다. 보호조류는 노량부리백로(Egretta eulophotes), 원앙(Aix galericulata), 물수리(Pandion haliateus), 흰목물떼새(Charadrius placidus) 등 총 4종이 관찰되었다. 물새류 서식의 방해요인으로는 해안도로의 건설 및 차량과 사람들의 출입 등이 관찰되었다. We researched status of waterfowl of Gujwa region where offshore wind farm was planed in Jejudo Island from March 2006 to February 2007. As the result of, a total of 16,786 individuals of 58 species were observed and the species diversity was 1.735. The dominant species were Larus argentatus (51.36%), Larus crassirostris (21.60%), Phalacrocorax capillatus (5.53%), Calidris alpina (4.46%) and Calonectris leucomelas (3.74%). Monthly observed species were the maximum as 36 species in April but the minimum as 8 species in June, and individuals were the maximum as 7,972 birds in February but the minimum as 33 birds in June. Protective birds were observed a total of 4 species including Egretta eulophotes, Aix galericulata, Pandion haliateus and Charadrius placidus. A disturbance factors of inhabitation of waterfowl were observed the construction of a cost road and coming and going of vehicle and person etc.

CASE REPORTS : Agranulocytosis Induced by Ethambutol in a Patient with Pulmonary Tuberculosis

( Kyoung Min Moon ),( Min Soo Han ),( So Hee Chung ),( Ju Ri Kim ),( Jin Young Kim ),( Sun Young Jung ),( Yong Seon Cho ) 대한결핵 및 호흡기학회 2015 Tuberculosis and Respiratory Diseases Vol.78 No.2

We report a case of agranulocytosis caused by ethambutol in a 79-year-old man with pulmonary tuberculosis. He was referred for fever and skin rash developed on 21th day after antituberculosis drugs (isoniazid, rifampicin, ethambutol, and pyrazinamide) intake. Complete blood count at the time of diagnosis of pulmonary tuberculosis was normal. On the seventh admission day, agranulocytosis was developed with absolute neutrophil count of 70/μL. We discontinued all antituberculosis drugs, and then treated with granulocyte colony-stimulating factor. Three days later, the number of white blood cell returned to normal. We administered isoniazid, pyrazinamide, and ethambutol in order with an interval. However, fever and skin rash developed again when adding ethambutol, so we discontinued ethambutol. After these symptoms disappeared, we added rifampicin and ethambutol in order with an interval. However after administering ethambutol, neutropenia developed, so we discontinued ethambutol again. He was cured with isoniazid, rifampicin, and pyrazinamide for 9 months.

Solitary Bronchial Squamous Papilloma Presenting as a Plaque-like Lesion in a Subject with Asthma.

Kim, So Ri,Park, Jin Kyeong,Park, Seoung Ju,Min, Kyung Hoon,Lee, Min Hee,Han, Hyo Jin,Chung, Chi Ryang,Choi, Kyoung Hwa,Chung, Myung Ja,Lee, Yong Chul American Lung Association 2011 American journal of respiratory and critical care Vol.183 No.4

Transfusion Related Acute Lung Injury after Cesarean Section in a Patient with HELLP Syndrome

Kyoung Min Moon,Min Soo Han,Ch`ang Bum Rim,So Ri Kim,Sang Ho Shin,Min Seok Kang,Jun Ho Lee,Jihye Kim,Sang Il Kim 대한가정의학회 2016 Korean Journal of Family Medicine Vol.37 No.1

Transfusion-related acute lung injury (TRALI) is a serious adverse reaction of transfusion, and presents as hypoxemia and non-cardiogenic pulmonary edema within 6 hours of transfusion. A 14-year-old primigravida woman at 34 weeks of gestation presented with upper abdominal pain without dyspnea. Because she showed the syndrome of HELLP (hemolysis, elevated liver enzymes, and low platelet count), an emergency cesarean section delivery was performed, and blood was transfused. In the case of such patients, clinicians should closely observe the patient’s condition at least during the 6 hours while the patient receives blood transfusion, and should suspect TRALI if the patient complains of respiratory symptoms such as dyspnea. Furthermore, echocardiography should be performed to distinguish between the different types of transfusion-related adverse reactions.