백서 혈관평활근 세포에서 α-Lipoic acid가 PAI-1 발현, 세포의 증식, 주유능 및 신생내막 형성억제에 미치는 효과

신동우,이동욱,이상준,김혜순,강효경,안종덕,이인규 대한당뇨병학회 2002 Diabetes and Metabolism Journal Vol.25 No.6

연구배경:당뇨병의 혈관 합병증의 발생에 있어서 산화스트레스는 중요한 역할을 하는 것으로 알려져 있다. 혈관 평활근세포에서 고혈당은 안지오텐신Ⅱ와 더불어 활성산소족(ROS)을 증가시키며, 산화스트레스에 민감한 전사인자들을 활성화시켜 동맥경화증을 유발하는 것으로 알려져 있다. 이에 저자들은 항산화제로 알려진 알파­리포산이 혈관 평활근세포의 증식속도와 이주에 미치는 영향과 PAI­1발현에 미치는 영향을 조사하였고 이와 동시에 백서의 혈관 손상 모델을 이용하여 알파­리포산이 신생내막 증식을 억제시킬 수 있는지를 알아보았다. 방법:In vitro 실험은 백서의 대동맥 평활근세포를 고농도의 포도당(22mM)과 100nM의 안지오텐신Ⅱ로 4시간 배양하였다. 알파­리포산을 처리 후, PAI­1 mRNA의 발현을 보기 위해 노던 블롯을 시행하였고 평활근세포의 유주능과 증식속도에 미치는 영향을 보기 위해 성장속도의 유주능 분석을 시행하였다. 또한 평활근세포의 유주능에 NF­μB 경로가 미치는 영향을 보기 위해 겔 지연 분석과 NF­μB 보고 유전자 분석을 시행하였다. In vivo 실험으로 백서의 혈관손상 모델을 이용하여 알파­리포산 처리 후 혈관의 신생내막 두께를 비교하였다. 결과:In vitro 실험에서 고농도 포도당과 안지오텐신Ⅱ에 의해 유도된 PAI­1 mRNA 발현증가는 알파­리포산 처리에 의하여 용량에 비례하여 억제되었다(p<0.05). 알파­리포산 처리로 혈관 평활근세포의 유주능은 유의하게 억제되었으나(p<0.01),증식속도는 유의하게 억제되지 않았다. 또한 알파­리포산 처리로 NF­μB 발현도 유의하게 억제되었다(p<0.01). In vivo 실험에서 알파­리포산을 주입한 군에서 혈관손상에 의한 신생내막의 증식이 유의하게 억제되는 것을 보여주었다(p<0.01). 결론:알파­리포산은 백서의 대동맥 평활근 세포의 증식속도는 억제하지 못했으나 유주능은 유의하게 억제하였으며, 혈관 손상 모델에서 신생 내막 증식도 유의하게 억제함을 확인할 수 있었다. 이러한 작용은 알파­리포산에 의한 NF­μB 경로의 억제와 연관이 있는 것으로 사료된다. Background : Exposure to large amounts of glucose causes a characteristic dysfunction and morphologic changes of the endothelium by an increased production of reactive oxygen species(ROS) in diabetes. The plasminogen activator inhibitor-1(PAI-1), which modulates fibrinolysis and cell migration may influence proteolysis and neointimal formation in vascular smooth muscle cells(VSMC). Antioxidants have been proposed to inhibit multiple proatherogenic events. This study investigated the effect of α-lipoic acid on PAI-1 expression and VSMC proliferation and migration both in vivo and in vitro. Methods : In the in vitro study, cultured rat aortic smooth muscle cells(RASMC) were incubated in a medium containing high glucose (22 mM) and 100 nM angiotensin Ⅱ for 4 hour. After α-lipoic acidtreatment, a -migration and growth assay of the RASMC, and a gelmobility shift assay and reportergene analysis for nuclear factor- иB(NF- иB) and northern blot analysis for PAI-1 were performed. In the in vivo study, the effect of α-lipoic acid on neointimal hyperplasia in a rat carotid balloon injury model was evaluated. Results : RASMC migration was inhibited significantly by α-lipoic acid (p<0.01), but their prolife ration was not inhibited. The NF-иB DNA binding activity and NF-иB promoter activity was inhibited by α-lipoic acid significantly (p<0.01). α-lipoic acid inhibited PAI-1 mRNA expression by high glucose and angiotensin Ⅱ in dose dependent manner (p<0.05). In the rat carotid artery balloon injury model, neointimal formation was reduced by α-lipoic acid treatment in a dose dependent manner significantly (p<0.01). Conclusion : α-lipoic acid suppresses migration, but not prolife ration in RASMC. α-lipoic acid also reduce neointima formation in a rat carotid balloon injured model. This effect might be related to the blocking of NF-иB which increase the expression of the genes associated with atherosclerosis including TNF-α, IL-1, IL-6, endothelin-1, MCP-1, VCAM-1, ICAM-1, E-selectin, tissue factor(J Kor Diabetes Asso 25:446~459, 2001).

Sol-Gel Process를 利用한 Y-SrTiO_3 微分體 合成

申孝順,이병교 경북대학교 산업기술연구소 1992 産業技術硏究誌 Vol.20 No.-

Y-SrTiO_3 powders are synthesized by pechini process as a kind of sol-gel process and compared with powders by solid state reaction with respect to powder properties. As a result, calcination temperature is lowered about at 500℃. Furthermore, obtained powders are fine and homogeneous. And these powders are agglomerated and the size of primary particles is about 30㎚.

중학교 남녀학생별 가정교과에 대한 인식 및 학습효과 : 경남지역을 중심으로

신동순,김상희,오화자,정효숙,정혜경 한국 가정과 교육 학회 1997 한국가정과교육학회지 Vol.9 No.1

The aims of this research study is to clear the differences of the learning effects and the cognition of Home Economics by sex in the middle school and to collect the future-oriented educational datas, after implementing the 6th education curriculum. The results of the study are as follows ; 1. Most of students had the cognition that Home Economics is a subject matter of a needful knowledge and skill for the family life, half students a cultural subject matter for the modern life. The former was supported by female students, the latter by male students. 2. Most of students were interested in actual training fields of cooking and hand-sewing, had a strong interest about a field of physical and social-psychological growth. The differences by sex were statistically significant in these fields. 3. Most of students had positive responses in the learning effects of Home Economics. The most useful field was cooking and the worst was the structure and method of sewing machine. 4. The shortage and the dissatisfaction in the learning of Home Economics were lacking of the actual training hours, the old-fashioned teaching methods and the shortage of teaching materials etc.

Fosfomycin의 항균효과 및 capillary electrophoresis에 의한 측정법에 관한 연구

윤효인,박승춘,이규승,권진욱,조준형,신광순,김무열,허강준,조명행 충남대학교 수의과대학 동물의과학연구소 1996 動物醫科學硏究誌 Vol.4 No.-

Fosfomycin, a low molecular antibiotic, is to be easily synthesized, therefore to be possibly used in veterinary sectors due to econimic reasons. In this study, we determined the antibacterial activities of fosfomycin against some domestic animal-origin pathogens, and its combined effects with some important antibacterials available. We also compared detection methods of fosfomycin from various measuring instruments. The results obtained through this experiment were as follows: 1. Antibacterial activities of fosfomycin against the pathogens used in this experiment were appeared fairly low. We found the antobacterial activities depanded on the kinds of media and the addition of some material thereins, which suggests the importance of appropriate medium so as to judge the antibacterial activity of fosfomycin. 2. Fosfomycin showed varying antibacterial effects in combination with other antibacterials, which should be taken into consideration when combining other antobacterials especially in the clinical settings. Of the combined antivacterials, fosfomycin had synergistic effects in combination with amoxicillin but antagonistic effects with oxytetracyline. 3. Conventional detection methods using HPLC, GC and spectrophotometer were not appropriate to measure fosfomycin. Capillary electrophoresis by the way of the indirect ultraviolet detection method was good enough to detect fosfomycin in the range of expected concentration in tissues, with the limit of quantitation (LOQ) of 1.25 ㎍/㎖. We recommend the eletrophoresis method could be used to detect fosfomycin, thereby being able to set the optimal dosage for animals and providing the measures in relation with the residual concentration in the meat.

증발과 응결에 대한 국민 학생들의 개념 조사

최병순,김효남,강순희,신인철 한국교원대학교 과학교육연구소 1993 청람과학교육연구논총 Vol.3 No.1

The purpose of this study was to investigate and analyze the conception of elementary school students on evaporation and condensation, and then to verify the types of the conception. Forty-eight children from six elementary schools were sampled by stratified random sampling in Seoul and other provinces. They responded to the questionaire and the interview. This study was carried out according to the process of the basic study, the preliminary study, and the main study. The materials collected were classified and analyzed according to the types of children's ideas. The findings of this study were as follows: 1. The vocabulary used to describe the evaporation phenomena varied according to the situations, and the scientific term "evaporation" was more frequently used by the older groups. 2. Most children answered that the last location of water were air/sky/cloud. Air/cloud which represents the scientific conception of the location of water were mentioned by children of all ages. The higher the grade of the children were, the more scientific conception the children mentioned, however. 3. Most children referred to the heat as the factor of evaporation. Wind, on the other hand was mentioned by less than 10% of lower graders, and by about 30% of higher graders. 4. The result of asking children whether they thought it was possible to get the evaporated water back showed that about 70% of lower graders denied the possibility of the water being reversible. About 60% of higher graders, however, recognized that the water will be returned as rain or condensation. This increase may be associated with formal teaching of water cycle. 5. In the ideas of evaporation and condensation, some of the children have supernatural ideas and animism, which are most younger chilren's characteristics.

돼지에서 정맥, 근육 그리고 경구 투여시의 enrofloxacin의 약물동태학

윤효인,김무열,박승춘,조준형,박병권,이내경,노상석,장범수,신광순,조명행 충남대학교 수의과대학 동물의과학연구소 1996 動物醫科學硏究誌 Vol.4 No.-

In order to characterize pharmacokinetic profiles according to route of a new enrofloxacin salt form (Enrotil®), it was given to 4 healthy pigs via oral (p.o.), intramuscular (i.m.) or intravenous (i.v.) administrations at a dose rate of 5 ㎎/㎏ body weight. Enrofloxacin (ENFX) in serum was detected by bioassay using E. coli BE1186 as a test organism. The biological elimination half-lives (t_1/2(β)) of ENFX were 6.76±0.99 h (i.v.), 7.16±2.30 h (i.m.) and 11.45±3.90 h (p.o.), Volume of distribution (Vd) of enrofloxacin was 2.20±0.31 L/㎏ (i.v.), 2.52±0.60 L/㎏ (i.m.) and 1.88±0.33 L/㎏ (i.m.). Mean residence time (MRT) was 8.77±1.26 h after i.v. injection and the maximal concentration time (Tmax) following p.o. and i.m. administration was 0.76±0.09 h and 0.60±0.12 h, indicating a rapid absorption from these routes. Bioavailibility (F) was calculated as 64.1% for p.o. administration and 59.71% for i.m. injections. In summary, the newly formulated enrofloxacin salt form has shown a high water solubility, rapid absorption and large tissue distribution, suggesting a potential antibacterials for oral application on a large scale in veterinary sectors.

열환경 개선을 위한 전통민가의 보존적 개수방안 연구 : 외암마을 건재고택 안채를 대상으로

김명신,김준태,박효순 대한건축학회 2004 대한건축학회논문집 Vol.20 No.7

Traditional houses as cultural assets are required to preserve their original forms, which could conflict with residents' demand for higher living standards. These houses were built with sustainable structure and material that can contribute to natural environmental control of building. Nevertheless, their thermal environment seem to be inferior in modern living standards and improvements are certainly necessary. The aim of the study is to evaluate the effects of the building improvements on the energy performances of a traditional house. In this study, the simulation program of Visual DOE 3.1 was used to predict annual heating and cooling energy loads of the house, and sensitivity studies were performed. The subject building in this study was a main building of Genjaegotaek, located at Oeam-maeul, Asan, Chung-Nam. The simulation results for that building indicated that the main determinants of the building energy loads were walls and openings(windows and doors): They represented about 50% and 41% of the annual heating loads, and 49% and 24% to the annual cooling loads, respectively. The improvements of wall and openings turned to be more effective in the house. Of the three measures of energy improvements, wall insulation is the most relatively important, followed by openings, and then ceiling insulation. The energy simulation was further performed with all three improvement measures, and the results indicated that the building heating and cooling loads decreased about 66% and 13%, respectively, and the total annual energy loads decreased by about 46%. This study addressed the issue of thermal improvements of traditional house through building energy simulations. Due to some unknown properties of building elements there are some limitations in modeling the traditional house, and more detailed information from surveys and experiments is needed for more reliable thermal modeling.

토끼에서 enrofloxacin과 enrofloxacin acetate의 比較藥物動態學

윤효인,박승춘,김창식,김민규,최기섭,신광순,박종일,조준형,조명행 충남대학교 수의과대학 동물의과학연구소 1996 動物醫科學硏究誌 Vol.4 No.-

This study was designed to examine the in vitro antibacterial activities and pharmacokinetics of enrofloxacin acetate (ENFXA) and enrofloxacin (ENFX). Two enrofloxacins had wide spectrum antibacterial activities against Gram positive and negative bacteria, and mycoplasma, showing potent antibacterial activities with low MICs (0.05-3.33 ㎍/㎖ for Gram positives, 0.002 ㎍/㎖ for Gram negatives and 0.02 ㎍/㎖ for mycoplasma). In order to investigate the profiles of two formulations of enrofloxacin we studied comparative pharmacokinetics of them in rabbits. Five rabbits were given 5 ㎎/㎏ body weight of each enrofloxacin formulation intravenously (i.v.) and orally (p.o.) in a cross-over study. Pharmacokinetic parameters of both formulations were calculated by the use of PCNONLIN, a computer program. Their bioavailibility in rabbits, the means of the area under the curve (AUC). After oral administration of each formulation in rabbits, the mean elimination half-lives (t_1/2,ke) were 3.25h (ENFX) and 4.32 h (ENFXA), and mean AUC 4.27 ㎍·h/㎖ (ENFX) and 6.21 ㎍·h/㎖ (ENFXA). Both enrofloxacin formulations seemed to have good tissue distribution and penetration as indicated by large volume of distribution: 4.76 1/㎏ for ENFX and 7.23 1/㎏ for ENFXA. With the results obtained in this study, ENFXA could be used in place of ENFX in rabbits.

이온 펌프의 제작과 특성에 관한 연구

박종윤,이순보,부진효,신익조 성균관대학교 기초과학연구소 1990 論文集 Vol.41 No.1

By using theoretical model suggested by H. Hartwig et al., performance of sputter ion pump (SIP) was calculated and a SIP was constructed. Measurement of pumping speed of the SIP has been performed with the differential pumping method. The pumping speed for N_2 gas is 48 l/s, and for Ar gas is 20 l/s. These results agree with the theoreticaly expected values.

16 Bit Microprocessor를 이용한 유도전동기의 최적제어에 관한 연구

徐基永,金兌孝,權純杰,申千雨 慶南大學校 附設 工業技術硏究所 1989 硏究論文集 Vol.7 No.-

本 論文은 16 bit one-chip 마이크로프로세서를 이용하여 正弦波 變調 最適 PWM 制御方式을 채택한 인버어터에 관한 연구이다. 3相 誘導電動機의 可變速 運轉에서 스위칭시에 발생되는 損失의 증가 및 效率 저하의 원인이 되는 高周波成分과 이를 除去하기 위한 알고리즘을 해석하고,이 알고리즘을 실현하기 위해 制御用 16 bit one-chip 마이크로프로세서로 구성되는 hardware시스템을 設計함으로써 종래의 마이크로프로세서로 실현 불가능했던 on-line 계산 및 real-time 실현을 가능하게 하였다.이 시스템을 最適으로 制御하고 計算하기 위한 software를 개발하고,아울러 외부 시스템과 RS-232C로 통신할 수 있는 기능을 첨부하여 host computer에서 remote control이 가능하게 함으로써 MAP(manufacture automation protocol)에 대처할 수 있게 하였다. hardware 및 software의 設計에 따라 제작된 시스템의 高주波 制御效果를 확인하기 위한 實驗에서 現在 産業界에서 사용되고 있는 우수한 성능을 가진 것으로 알려진 特定모델을 선정하여 이와 比較 檢討하였다. 그 結果 인버어터의 基本波成分과 각 高調波成分의 스펙트럼이 出力周波數에 따라 다소의 차이가 있었으나, 測定 周波數의 전체범위에서 比較된 특정모델보다 우수한 特性을 얻을 수 있었다. This paper describes an inverter adopting an optimal sinusoidal waveform modulation PWM control method using 16 bit one-chip microprocessor. In variable speed operation of three-phase induction motor, a large number of harmonic components are included in switching waveform and cause decrement of efficiency. We analyzed some properties of each harmonic component and an algorithm for elimination of harmonics. In order to implement the algorithm, we designed a hardware system composed of special purpose 16 bit one-chip microprocessor. But it has been impossible to get a real-time computation. We compared and examined the effect of harmonics between the designed inverter and the commercial inverter. It was found that the designed inverter had more excellent suppression of harmonics than the selected commercial inverter over a range of all measured frequencies.