위장관계 악성종양에서 CA 125 측정의 의미

장우익 ( Jang U Ig ),안강현 ( An Gang Hyeon ),이종인 ( Lee Jong In ),김현수 ( Kim Hyeon Su ),김효열 ( Kim Hyo Yeol ),김수경 ( Kim Su Gyeong ),이동기 ( Lee Dong Gi ),배선우 ( Bae Seon U ),심영학 ( Sim Yeong Hag ),강명서 ( Kang Myeo 대한내과학회 1993 대한내과학회지 Vol.44 No.4

연구배경 : 종양표지자는 암외 진단, 임상적 병기구분 및 치료효과 판정에 유용하며, 위장관암에서 alphafetoprotein, carcinoembryonic antigen, CA 19-9, 및 CA 72-4 등이 중요한 지표로 사용되고 있으나 위암의 경우, 현재까지 만족할 만한 유용성을 가진 종양표지자는 없는 실정이다. 저자등은 위암에 대한 새로운 종양표지자로 CA 125의 역할을 규명하고자 위암을 비롯한 각종 위장관암에서 CA 125와 기존의 종양표지자와의 유용성을 검토하였다. 방법 : 대상은 위장관 악성종양 181예, 위장관 양성질환자 55예를 대상으로 하였으며 CA 72-4는 CIS ELSA CA 72-4 RIA kit, CA 19-9는 Abbott CA 19-9 RIA kit, CEA는 Abbott CEA kit, CA 125는 Centocor CA 125 RIA kit를 사용하여 측정하였으며, 각각의 정상치 한계는 4 U/ml, 37 U/ml, 5ng/ml그리고 35 U/ml로 하였다. 결과 : 1) CA 125는 양성 소화기 질환에서 93%의 높은 특이도를 보였으나 복수가 있는 간경변증의 경우 거의 전예에서 혈중 CA 125값이 증가하여 악성종양군과 감별이 어려웠다. 2) CA 125는 위암에서 민감도가 36%로 CA 72-4와 차이가 없었다. 3) CA 125는 췌담도암에 대한 민감도가 55%로 CA 19-9의 85%에 비해 낮았으며 두 종양표지자를 병합하여 사용한 경우 민감도의 증가(95%)를 볼 수 있었다. 4) 23예의 조기위암 환자에서 CA 125의 민감도는 9%로 낮았다. 5) Conventional image study 상으로 인지되지 않은 복강내 암종증 환자에 대한 CA 125의 민감도는 36%였다. 결론 : CA 125는 위암에서 기존의 종양표지자인 CA 72-4와 유용성의 차이를 보이지 않았으며 전이성 위암에서 높은 민감도를 나타내었다. 혈중 CA 125의 증가는 위장관의 악성 및 양성질환에 관계없이 복수의 유무와 밀접한 연관관계를 가지는 것으로 사료되며 통상적인 영상 진단법상으로 인지되지 않은 복강내 암종중의 예견에 있어서는 유용하지 않을 것으로 생각된다. Background: Detection of tumor markers could offer an accessible method for screening risk groups in order to achieve an early diagnosis of cansers, to contribute to and adequate staging, and to help evaluate effects of therapy. CA 125 is an antigenic determinant defined by murine monoclonal antibody OC 125, and synthesized by coelomic epithelial derivatives such as Mullerian epithelium and mesothelial tissues. CA 125 is regarded as useful tumor marker for non-musinous epithelial ovarian tumors. So we investigated whether CA 125 is reliable tumor marker for gastrointestinal malignancies and especially for detection of intraperitoneal carcinomatosis unrecognized by conventional imaging studies. And we compared CA 125 with other conventional tumor markers in the serodiagnosis of gastrointestinal malignancies. Methods: Serum CA 72-4, CA 19-9, CA 125 and CEA were determined radioimmunologically with monoclonal antibodies. A cut-off value of 4 U/ml, 37 U/ml, 35 U/ml and 5 mg/ml were used respectively. Results: The results are summarized as follows. 1) CA 125 had high specificity (93%) for benign gastrointestinal disease but was not useful to differetiate malignant ascites and benign ascites. 2) In gastric carcinoma, the sensitivity of CA 125 (36%) was not so different to that of CA 72-4(36%). 3) In pancreatobiliary carcinoma, the sensitivity of CA 125(55%) was inferior than that of CA 19-9 (85%), but combined use of these two tumor markers increases sensitivitu(95%) for the diagnosis. 4) The sensitivities of CA 125 for 23 early gastric cancer was 9%. 5) The sensitivities of CA 125 for the intraperitoneal carcinomatosis that was not recognized by conventional imaging studies was 36%. Conclustion: Our data indicates that CA 125 is not better in the serodiagnosis of gastrointestinal malignancies than other conventional tumor markers, and CA 125 has a little predictive value in the diagnosis of intraperitoneal carcinomatosis unrecognized by the conventional imaging studies. Serum CA 125 elevation in benign or malignant diseases closely correlated with the presence of ascites.

SIRT1 is required for oncogenic transformation of neural stem cells and for the survival of “cancer cells with neural stemness” in a p53-dependent manner

Lee, Ji-Seon,Park, Jeong-Rak,Kwon, Ok-Seon,Lee, Tae-Hee,Nakano, Ichiro,Miyoshi, Hiroyuki,Chun, Kwang-Hoon,Park, Myung-Jin,Lee, Hong Jun,Kim, Seung U.,Cha, Hyuk-Jin Oxford University Press 2015 Neuro-oncology Vol.17 No.1

<P><B>Background</B></P><P>Cancer stemness, observed in several types of glioma stem cells (GSCs), has been demonstrated to be an important barrier for efficient cancer therapy. We have previously reported that cancerous neural stem cells (F3.Ras.CNSCs), derived from immortalized human neural stem cells by a single oncogenic stimulation, form glial tumors in vivo.</P><P><B>Method</B></P><P>We searched for a commonly expressed stress modulator in both F3.Ras.CNSCs and GSCs and identified silent mating type information regulation 2, homolog (SIRT1) as a key factor in maintaining cancer stemness.</P><P><B>Result</B></P><P>We demonstrate that the expression of SIRT1, expressed in “cancer cells with neural stemness,” is critical not only for the maintenance of stem cells, but also for oncogenic transformation. Interestingly, SIRT1 is essential for the survival and tumorigenicity of F3.Ras.CNSCs and GSCs but not for the U87 glioma cell line.</P><P><B>Conclusion</B></P><P>These results indicate that expression of SIRT1 in cancer cells with neural stemness plays an important role in suppressing p53-dependent tumor surveillance, the abrogation of which may be responsible not only for inducing oncogenic transformation but also for retaining the neural cancer stemness of the cells, suggesting that SIRT1 may be a putative therapeutic target in GSCs.</P>

전열관의 굽힘 및 확관접합 잔류응력

장진성,배강국,김우곤,김선재,국일현,김성청,Jang, Jin-Seong,Bae, Gang-Guk,Kim, U-Gon,Kim, Seon-Jae,Guk, Il-Hyeon,Kim, Seong-Cheong 대한기계학회 2000 大韓機械學會論文集A Vol.24 No.2

Residual stress induced in U-bending and tube-to-tubesheet joint processes of PWR's row-1 heat exchanger tube was measured by X-ray method and Hole-Drilling Method(HDM). Compressive residual stresses(-) at the extrados surface were induced in U-bending, and its maximum value reached -319 MPa in axial direction at the position of $\psi$ = $0^{\circ}$. Tensile residual stresses(+) of $\sigma_{zz}$ = 45 MPa and $\sigma_{\theta\theta}$ = 25 MPa were introduced in the intrados surface at the position of $\psi$ = $0^{\circ}$. Maximum tensile residual stress of 170 MPa was measured at the flank side at the position of $\psi$ = $90^{\circ}$, i.e., at apex region. It was observed that higher stress gradient was generated at the irregular transition regions (ITR). The trend of residual stress induced by U bending process of the tubes was found to be related with the change of ovality. The residual stress induced by the explosive joint method was found to be lower than that by the mechanical roll method. The gradient of residual stress along the expanded tube was highest at the transition region (TR), and the residual stress in circumferential direction was found to be higher than the residual stress in axial direction.

HPLC에 의한 柑橘中의 遊離糖 定量

金瑛淑,康順善,高正殷,柳長杰 제주대학교 1985 논문집 Vol.21 No.-

HPLC에 의한 分析에서 가장 많은 經費를 부담케하는 것중의 하나가 容媒이기 때문에 이와같은 점에서 柑橘汁중의 遊離糖 分析을 하는데 관련되는 몇가지 조건을 檢討한 結果는 다음과 같았으며 사용 機器는 Waters 회사 제품의 본체에 μBondapak NH₂column, RI detector (x8)이었고 용매로는 acetonitrile/H₂O system을 사용했다. 1. 75%의 acetonitrile 농도에서 1.0㎖/min의 유속이 容媒를 절약하는데 가장 경제적인 조건이었다. 2. 시료중의 불순물을 제거하기 위해서 Sep-Rak C?? cartridge를 사용할 경우 糖시료를 한번 통과시킨 뒤에 4㎖의 H₂O로 세척하면 재사용이 가능했다. H₂O로 세척된 cartridge에는 다음 시료를 3㎖ 통과 시킨뒤에 나오는 시료액을 injection sample로 사용하면 안전하다. 3. 시료주입량을 보면 fructose, glucos,sucrose 모두 140㎍까지 주입해도 직선상의 결과를 얻을 수 있었다. 4. 본 실험 조건의 HPLC에 의한 糖분석에서 glucose와 mannitol의 분리가 좋지 않으므로 내부표준물질로 mannitol을 사용하는 것은 부적당하다. In order to minimize the cost of sugar analysis of mandarin juice by HPLC, some conditions related were discussed using Waters, M246 HPLC main body with R-401 RI-detector(x8), M510 pumping system, U6K injector, M730 Data Module, μBondapak NH₂column (3.9mm*30cm), Sep-Pak C?? cartridge and acetonitrile/H₂O solvent system. 1) The most suitable conditions to save acetonitrile solvent were obtained at 1.0ml/min of the flow rate of 75% acetonitrile in H₂O. 2) The Sep-Pak C?? cartridge, used once, and be washed out by using 4ml of H₂O under the conditions of the present study. When the washed Sep-Pak cartridges wetted with H₂O were passed through with 3ml of the new sugar sample, there was no dilution effect found and nearly 100% sugar yield in the filtrate. 3) Up to 140㎍ of sugars (fructose,glucose, sucrose) there was good linear relationship bwteen the injected amounts and the analyzed values. 4) It was found that mannitol can not be used as the internal sugar standard because of overlapping with glucose chromatogram in HPLC under the present conditions.

전치부 반대교합을 동반한 환자에서의 수직고경 회복

계기성,최우식,이선아 조선대학교 구강생물학연구소 2002 Oral Biology Research (Oral Biol Res) Vol.26 No.2

In cases of anterior crossbite patients, there are many problems. They are dissatisfied with facial appearance and in difficulties for speaking, and sometimes are related to reduced vertical dimension. Vertical dimension is defined distances between two points which one is on maxilla and the other is on mandible. Loss of vertical dimension may causes temporomandibular disorder, loss of masticatory function, and collapse of occlusion. This is a clinical study of 50 years old male with reduced vertical dimension and anterior-crossbite. We treated patient with fixed bridge and conventional removable partial denture, and then the activity of masseter and temporalis muscle were recorded and analyzed by means of Biopak?? system(Bioresearch Inc., Millwakee Wisconsin). The result was satisfactory functionally and esthetically.

子宮內膜搔爬術 21例의 鍼麻醉效果에 대한 臨床的 硏究

金在圭,金相佑,李宜暻,金建植,金相佑,南相永 경희대학교 1997 慶熙醫學 Vol.13 No.1

크롬계 탄화물의 분포와 형태가 니켈계 합금 600의 염기응력부식에 미치는 영향

김선재,최종호,성진경,김우곤,박순동,이창규,정용환,국일현,Kim, Seon-Jae,Choe, Jong-Ho,Seong, Jin-Gyeong,Kim, U-Gon,Park, Sun-Dong,Lee, Chang-Gyu,Jeong, Yong-Hwan,Guk, Il-Hyeon 한국재료학회 1998 한국재료학회지 Vol.8 No.8

32$0^{\circ}C$, 40%NaOH 용액의 autoclave에서 약 300wppm의 탄소를 함유하고 있는 15Cr-9Fe-balanced Ni 합금 판상시편에 대해 응력부식 저항성을 조사하였다. 부식시편은 $700^{\circ}C$, 100시간 동안의 열처리로 합금내부에 석출될 수 있는 가능한 한 많은 양의 크롬계 탄화물을 석출시킨 후, 다시 재용해에 의해 크롬계 탄화물의 형태를 조절하는 $800^{\circ}C$-$950^{\circ}C$범위의 최종열처리를 시행하고 급냉시킨 다음 U-자형으로 응력을 가하여 준비되었다. 최종열처리 온도가 올라감에 따라 시편들의 입계응력부식균열(IGSCC ) 전파속도는 $900^{\circ}C$까지는 거의 직선적으로 증가하다가 $950^{\circ}C$에서는 $700^{\circ}C$에서 얻은 값보다도 더 낮게 감소하였다. 즉, 크롬계 탄화물이 재용해되어 그 밀도가 감소함에 따라 IGSCC저항성이 감소하다가 완전히 재용해된 $950^{\circ}C$ 열처리 조건에서 오히겨 가장 큰 IGSCC 저항성을 나타내었다. 이와같은 최조열처리 온도에 따른 니켈계 합금 600의 부식거동은 입계에 존재하는 크롬계탄화물의 형태변화 때문이 아니라 입계에서 탄소-크롬계 탄화물-크롬간의 상평형에 의해 이루어지는 탄소의 입계편석량이 크롬계탄화물이 존재할 때에는 열처리 온도에 따라 증가하다가 그것이 완전히 재용해 되었을 때 가장 낮아지기 때문인 것으로 생각된다.

HBV : Retrospective Observational Study to Evaluate the Efficacy of Entecavir in Patients with Chronic Hepatitis B Virus Infection in Korea: Real-Life Clinical Outcomes

( Chung-Hwa Park ),( Sung Won Lee ),( Hee Yeon Kim ),( Do Seon Song ),( Hyun Jin Kim ),( Myeong Jun Song ),( Jung Hyun Kwon ),( Chan Ran You ),( Jeong Won Jang ),( U Im Chang ),( Chang Wook Kim ),( So 대한간학회 2013 춘·추계 학술대회 (KASL) Vol.2013 No.1

Background: Entecavir, has demonstrated superior histologic, virologic, and biochemical benefit. Still, its long-term clinical outcome is not well established in the Korean clinical area, and also in subjects who stopped the treatment. Methods: This is a multicenter, retrospective cohort observational study to evaluate the efficacy of Entecavir long-term treatment in CHB patients at hospitals affiliated to the Catholic University of Korea through analysis of the electronic medical record data from January 1, 2006 to December 31, 2012. Results: Among 2,440 patients prescribed with entecavir 0.5mg qd, 1,337 patients were treatment naive. Excluding 533 patients with concomitant conditions, 578 patients were ontreatment and 226 patient stopped entecavir during the study period. At 6mo, year 1, 2, 3, 4 and 5, cumulative incidences of complete virologic response (HBV-DNA < 300 copies/mL) was 379, 530, 573, 577, 579 and 579, respectively. HBsAg loss rate was 9.86%, and among 440 HBeAg-positive patients, HBeAg loss rate and HBeAg loss with HBeAb positivity rate was 20.00% and 17.43%, respectively, at year 1. During the study period, 226 patients stopped entecavir, and at year 1 after cessation, cumulative virologic relapse (HBV-DNA>10^4 copies/ mL) and biochemical relapse (ALT>40 U/L) were 22.57% and 20.35%, respectively with mean days of 191.06±67.0 and 188.39±90.15, respectively. Prognotsic factor for earlier CVR was HBV-DNA<10^7cpm at initiating entecavir (P<0.005). Among those who stopped the medication, prognostic factor for virological relapse was HBV-DNA ≥10^7cpm at initiating entecavir (P=0.022). Conclusions: Long term use of entecavir may achieve CVR in most patients, and patients with higher viral load should be considered of indefinite duration of treatment regardless of age, sex, biochemical markers or HBeAg status.

Hepatitis B Reactivation among Chronic Hepatitis C Patients Treated with Direct Acting Antivirals

( Hee Yeon Kim ),( Chang Wook Kim ),( Jin Ah Kim ),( Mi Ju Cheon ),( Chan Ran You ),( Sang Wook Choi ),( Se Hyun Cho ),( Joon-Yeol Han ),( Do Seon Song ),( U Im Chang ),( Jin Mo Yang ),( Sung Won Lee 대한간학회 2017 춘·추계 학술대회 (KASL) Vol.2017 No.1

Aims: This study aimed to investigate hepatitis B virus (HBV) reactivation among hepatitis C virus (HCV) infected patients treated with oral direct acting antiviral (DAA) therapy in areas endemic for HBV in Korea. Methods: A total of 821 HCV-infected patients receiving oral DAA therapy from March 2015 to November 2016 were evaluated. Serum HBV DNA and HCV RNA was regularly assessed during and after DAA treatment in patients with HBV-HCV coinfection. HBV virological reactivation was defined as a >1 log increase in HBV DNA from baseline or a reappearance of HBV DNA for those with baseline undetectable HBV DNA. Clinical reactivation was defined as a serum alanine aminotransferase level ≥3-fold over the upper limit of normal or > 100 U/L. Results: Prior to DAA therapy 95.8% (787/821) had HBsAg testing and 1.7% (13/787) were positive. HBV virological reactivation was found in 7 (53.8%) of the 13 patients. HBV-related clinical reactivation was not observed in our cohort. Conclusions: For HBV-HCV co-infected patients, the risk of HBV virological reactivation was present after oral DAA treatment for HCV infection. Monitoring the HBV DNA level during oral DAA therapy for HCV infection is warranted.

원자회선 분광기 광학계의 오차 예산 분석

Seon, K.-I.,Yuk, I. S.,Ryu, K.-S.,Park, J. H.,Jin, H.,Seon, J.-H.,Oh, S.-H,Rhee, J.-G.,Lee, D.-H.,Nam, U.-W.,Han, W.,Min, K.-W.,Lee, W.-B.,Edelstein, J.,Korpela, E. 한국우주과학회 2001 Journal of Astronomy and Space Sciences Vol.18 No.3

원자외선 분광기 (Far ultraviolet IMaging Spectres.mph; FIMS)는 과학위성 1호의 주탑재체로 개발되고 있다. 원자외선 분광기 광학부에 대한 민감도 분석과 오차 예산 분석을 광범위하게 수행하였다. 몬테카를로 시뮬레이션을 통하여 제작 오차 및 조립 오차 등의 모든 오차가 함께 존재할 때 실제 원자외선 분광기가 갖게 되는 성능을 조사하였다. 시뮬레이션 결과 과학 임무를 위해 필요한 모든 요구 조건을 99.9% 이상의 확률로 만족하고 있음을 확인하였다. Far-ultraviolet IMaging Spectrograph (FIMS) is under development as the main pay-load of the first Korean science satellite, KAISTSAT-4. An extensive sensitivity and error budget analyses of FIMS optical system have been performed. As an way of estimating aggregate effects of all tolerances, a Monte Carlo simulation is used. The simulation result shows that the optical performance required from the science objectives is achieved within the probability higher than 99.9%.