A Fruit Extract of Paeonia anomala Attenuates Chronic Alcohol-induced Liver Damage in Rats

Sarangerel Oidovsambuu,윤지호,강경수,Batsuren Dulamjav,Jigjidsuren Tunsag,남의정,노주원 한국생약학회 2016 Natural Product Sciences Vol.22 No.4

Prolonged alcohol consumption causes alcoholic liver damage due to the generation of reactive oxygen species, the accumulation of fatty acids, and an increase in inflammatory cytokines in the liver. In this study, the protective effect of a fruit extract of Paeonia anomala (FEPA) against chronic alcohol-induced liver damage was evaluated in Sprague-Dawley rats fed an ethanol or a control Lieber-DeCarli diet for 5 weeks to induce alcoholic liver damage. FEPA (50, 25, and 10 mg/kg body weight/day) as well as the reference control silymarin (25 mg/kg body weight/day) were administered along with the ethanol diet. FEPA protected against increases in alanine aminotransferase and aspartate aminotransferase in serum and attenuated alcohol-induced increases in triglycerides, tumor necrosis factor alpha, thiobarbituric acid-reactive substances, and cytochrome P450 2E1 enzyme activity in the liver compared with the group treated with ethanol only. Anti-oxidative defenses such as the total glutathione level and glutathione peroxidase activity were increased by FEPA treatment. These results suggest that FEPA exerts protective effects against chronic alcohol-induced liver damage by attenuating hepatosteatosis and pro-inflammatory cytokine production and enhancing anti-oxidative defense mechanisms in the liver.

Hepatoprotective Effect of Handaeri-gomchi (Ligularia fischeri var. spiciformis Nakai) Extract against Chronic Alcohol-induced Liver Damage in Rats

유지혜,Sarangerel Oidovsambuu,김상민,전나라,윤지호,강경수,조은혜,이샛별,노주원 한국식품과학회 2011 Food Science and Biotechnology Vol.20 No.6

Handaeri-gomchi (Ligularia fischeri var. spiciformis Nakai) (LF) is well known as a medicinal plant in Korea, especially to treat a variety of human disease including hepatic function failure. In this study, we examined the activity of LF extract against the liver injuries and oxidative stress by chronic alcohol in Spargue-Dawley rats. Severe liver damage caused by alcohol intake with increasing activity of hepatic markers was decreased in the group of rats fed LF extract. The results were confirmed through hematoxylin and eosin staining. Antioxidative capacity was decreased by alcohol but it was recovered by LF extract. Elevated indicators of oxidative stress by chronic alcohol were diminished in the group of LF extract. Furthermore, LF extracts increased antioxidative capacity and the activity of antioxidant enzymes. In conclusion, LF extract has a protective effect against chronic alcohol hepatotoxicity, suggesting it could be developed as a functional food or medicine for protection of liver disease.

Youngia denticulata Protects Against Oxidative Damage Induced by tert-Butylhydroperoxide in HepG2 Cells

강경수,조은혜,이희주,Sarangerel Oidovsambuu,윤지호,김철영,유지혜,김영진,김종환,안수영,노주원 한국식품영양과학회 2011 Journal of medicinal food Vol.14 No.10

Improvement of liver function is one of the most popular commercial health claims of functional foods in Asian countries, including Korea. After examining the potential of several traditional Korean wild vegetables for enhancing liver function, we found that Youngia denticulata Kitam. has strong hepatoprotective effects against oxidative stress induced by tert-butylhydroperoxide (t-BHP). We are the first to report that the extract and ethyl acetate fractions of Y. denticulata have radical scavenging activities and inhibit oxidative stress–induced cell death and DNA damage in HepG2 cells. The extract and ethyl acetate fractions significantly decreased cellular reactive oxygen species production and apoptosis induced by t-BHP in HepG2 cells. In addition, they prevented the depletion of cellular glutathione, which is an important defense molecule against oxidizing xenobiotics. Chlorogenic acid and 3,5-dicaffeoylquinic acid were found to be major active components responsible for the activity of Y. denticulata and could serve as marker compounds for standardization. These data suggest that Y. denticulata could be promoted as a potential antioxidative functional food candidate, particularly for hepatoprotection against oxidative stress.

Saposhnikovia divaricata root and its major components ameliorate inflammation and altered gut microbial diversity and compositions in DSS-induced colitis

Saruul Erdenebileg,Yang-Ju Son,Myungsuk Kim,Sarangerel Oidovsambuu,Kwang Hyun Cha,Jaeyoung Kwon,Da Seul Jung,Chu Won Nho 한국한의학연구원 2023 Integrative Medicine Research Vol.12 No.4

Background: The root of Saposhnikovia divaricata (Turcz.) Schischk is a well-known traditional medicinal plant, containing various bioactive compounds with anti-inflammatory, antioxidant, and analgesic properties. However, no scientific studies have validated its clinical use as an anti-inflammatory agent against inflammatory bowel disease (IBD). This study aimed to investigate whether the root extract of S. divaricata ameliorates IBD and induces gut microbial alteration, using a RAW 264.7 cell line and a DSS-induced colitis mouse model. Methods: To investigate the anti-inflammatory effects and alleviation of IBD, using a methanol extract of Saposhnikovia divaricata (Turcz.) Schischk. root (MESD), RAW 264.7, murine macrophages and a dextran sodium sulfate (DSS)-induced colitis mouse model were employed. 16S rRNA gene sequencing was conducted to determine the alterations in the gut microbiota of mice with DSS-induced colitis. Results: MESD significantly decreased nitric oxide (NO) and inflammatory cytokine levels in lipopolysaccharide (LPS)-induced RAW 264.7 cells in vitro. Oral administration of MESD reduced the expression of inflammatory cytokines in the colons of mice with DSS-induced colitis. Additionally, MESD inhibited the abundance of Clostridium sensu stricto 1 and enhanced the predicted functional pathways, including l-glutamate degradation VIII (to propanoic acid). Seven compounds with anti-inflammatory properties were isolated from the MESD. Among them, 3′-O-acetylhamaudol and 3′-O-angeloylhamaudol exhibited strong anti-inflammatory effects in vitro. Conclusion: Overall, MESD may be a potential natural product for the treatment of IBD by lowering inflammatory cytokine levels and altering gut microbiota composition.

Gymnaster koraiensis and its major components, 3,5-di-O-caffeoylquinic acid and gymnasterkoreayne B, reduce oxidative damage induced by tert-butyl hydroperoxide or acetaminophen in HepG2 cells

( Eun Hye Jho ),( Kyung Su Kang ),( Sarangerel Oidovsambuu ),( Eun Ha Lee ),( Sang Hoon Jung ),( Il Shik Shin ),( Chu Won Nho ) 생화학분자생물학회(구 한국생화학분자생물학회) 2013 BMB Reports Vol.46 No.10

We investigated the protective effects of Gymnaster koraiensis against oxidative stress-induced hepatic cell damage. We used two different cytotoxicity models, i.e., the administration of tert-butyl hydroperoxide (t-BHP) and acetaminophen, in HepG2 cells to evaluate the protective effects of G. koraiensis. The ethyl acetate (EA) fraction of G. koraiensis and its major compound, 3,5-di-O-caffeoylquinic acid (DCQA), exerted protective effects in the t-BHP-induced liver cytotoxicity model. The EA fraction and DCQA ameliorated t-BHP-induced reductions in GSH levels and exhibited free radical scavenging activity. The EA fraction and DCQA also significantly reduced t-BHP-induced DNA damage in HepG2 cells. Furthermore, the hexane fraction of G. koraiensis and its major compound, gymnasterkoreayne B (GKB), exerted strong hepatoprotection in the acetaminopheninduced cytotoxicity model. CYP 3A4 enzyme activity was strongly inhibited by the extract, hexane fraction, and GKB. The hexane fraction and GKB ameliorated acetaminophen-induced reductions in GSH levels and protected against cell death. [BMB Reports 2013; 46(10): 513-518]

Antioxidant Activity of Phenolics in Leaves of Three Red Pepper (Capsicum annuum) Cultivars

Kim, Woo-Ri,Kim, Eun Ok,Kang, Kyungsu,Oidovsambuu, Sarangerel,Jung, Sang Hoon,Kim, Byung Sup,Nho, Chu Won,Um, Byung-Hun American Chemical Society 2014 Journal of agricultural and food chemistry Vol.62 No.4

<P>The antioxidant properties and phenolic profiles were first investigated in this paper on the leaves of three red pepper cultivars, Blackcuban (BCPL), Hongjinju (HPL), and Yeokgang-hongjanggun (YHPL). Of the ethanol extract of the three cultivars, BCPL showed potent antioxidant activities against the 1,1-diphenyl-2-picrylhydrazyl radical (DPPH) and the 2,2-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical. Nine antioxidative compounds from the red pepper leaves were isolated and identified as one polyamine phenolic conjugate, <I>N</I>-caffeoylputrescine (<B>1</B>); three chlorogenic acid derivatives, 5-<I>O</I>-caffeoylquinic acid (<B>2</B>), 5-<I>O</I>-caffeoylquinic acid methyl ester (<B>4</B>), and 5-<I>O</I>-caffeoylquinic acid butyl ester (<B>9</B>); one anthocyanin, delphinidin-3-[4-<I>trans</I>-coumaroyl-<SMALL>l</SMALL>-rhamnosyl(1→6)glucopyranoside]-5-<I>O</I>-glucopyranoside (<B>3</B>); and four flavone glycosides, luteolin-7-<I>O</I>-apiofuranosyl(1→2)glucopyranoside (<B>5</B>), luteolin-7-<I>O</I>-glucopyranoside (<B>6</B>), apigenin 7-<I>O</I>-apiofuranosyl(1→2)glucopyranoside (<B>7</B>), apigenin-7-<I>O</I>-glucopyranoside (<B>8</B>). <B>1</B> and <B>3</B> had the greatest potential for radical-scavenging activity and HepG2 cells protecting effect against oxidative stress. BCPL exhibited the highest content of <B>1</B> and <B>3</B>. Of the three cultivars BCPL may be considered a good source of antioxidants.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jafcau/2014/jafcau.2014.62.issue-4/jf403006c/production/images/medium/jf-2013-03006c_0005.gif'></P>

The Antimicrobial Activity of Essential Oil from Dracocephalum foetidum against Pathogenic Microorganisms

Saet Byoul Lee,Kwang Hyun Cha,김수남,Shataryn Altantsetseg,Sanduin Shatar,Oidovsambuu Sarangerel,Chu Won Nho 한국미생물학회 2007 The journal of microbiology Vol.45 No.1

A number of essential oils from Mongolian aromatic plants are claimed to have antimicrobial activities. The essential oil of Dracocephalum foetidum, a popular essential oil used in Mongolian traditional medicine, was examined for its antimicrobial activity. Eight human pathogenic microorganisms including B. subtilis, S. aureus, M. lutens, E. hirae, S. mutans, E. coli, C. albicans, and S. cerevisiae were examined. The essential oil of Dracocephalum foetidum exhibited strong antimicrobial activity against most of the pathogenic bacteria and yeast strains that were tested; by both the agar diffusion method and the minimum inhibitory concentration (MIC) assay (MIC range was 26-2592 μg/ml). Interestingly, Dracocephalum foetidum even showed antimicrobial activity against methicilin-resistant Staphylococcus aureus(MRSA) strains. We also analyzed the chemical composition of the oil by GC-MS and identified several major components, including n-Mentha-1,8-dien-10-al, limonene, geranial, and neral.

The Antimicrobial Activity of Essential Oil from Dracocephalum foetidum against Pathogenic Microorganisms

Lee, Saet-Byoul,Cha, Kwang-Hyun,Kim, Su-Nam,Altantsetseg, Shataryn,Shatar, Sanduin,Sarangerel, Oidovsambuu,Nho, Chu-Won The Microbiological Society of Korea 2007 The journal of microbiology Vol.45 No.1

A number of essential oils from Mongolian aromatic plants are claimed to have antimicrobial activities. The essential oil of Dracocephalum foetidum, a popular essential oil used in Mongolian traditional medicine, was examined for its antimicrobial activity. Eight human pathogenic microorganisms including B. subtilis, S. aureus, M. lutens, E. hirae, S. mutans, E. coli, C. albicans, and S. cerevisiae were examined. The essential oil of Dracocephalum foetidum exhibited strong antimicrobial activity against most of the pathogenic bacteria and yeast strains that were tested; by both the agar diffusion method and the minimum inhibitory concentration (MIC) assay ($MIC\;range\;was\;26-2592{\mu}g/ml$). Interestingly, Dracocephalum foetidum even showed antimicrobial activity against methicilin-resistant Staphylococcus aureus (MRSA) strains. We also analyzed the chemical composition of the oil by GC-MS and identified several major components, including n-Mentha-1,8-dien-10-al, limonene, geranial, and neral.