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Kwon, Jaeyoung,Kim, Nahyun,Lee, DongHyuk,Han, Ah-Reum,Lee, Jae Won,Seo, Eun-Kyoung,Lee, Je-Hyun,Lee, Dongho Elsevier 2014 Journal of pharmaceutical and biomedical analysis Vol.94 No.-
A liquid chromatography quadrupole time-of-flight mass spectrometry-based metabolomics approach was applied to metabolite profiling of Gastrodia elata in order to identify raw and steamed G. elata and explore potential biornarkers for each processing state. A statistical classification method, significant analysis of microarrays, was used to select influential metabolites from the different forms. Through metabolite selection, several potential biomarkers were determined and assigned by matching mass information with that of reference compounds or by comparing it with data in the literature. Furthermore, the developed method was cross-checked using two validation procedures. The first validation was performed simultaneously with the metabolite profiling of G. elata using all detected metabolites, and the second was performed after the metabolite profiling using representative standard compounds of G. elata. Overall, this study can be applied to quality assurance of G. elata. (C) 2014 Elsevier B.V. All rights reserved.
Kwon, Jaeyoung,Hiep, Nguyen Tuan,Kim, Dong-Woo,Hong, Sungeun,Guo, Yuanqiang,Hwang, Bang Yeon,Lee, Hak Ju,Mar, Woongchon,Lee, Dongho American Chemical Society and American Society of 2016 Journal of natural products Vol.79 No.8
<P>Seventy-five compounds, including 21 new compounds (1-21), were isolated from the root bark of Cudrania tricuspidata. The structures of the isolated compounds were elucidated by interpretation of their spectroscopic data. All isolated compounds were evaluated for their neuroprotective effects against 6-hydroxydopamine (6-OHDA)-induced cell death, and nine compounds had activities with EC50 values of 1.9-30.2 mu M. The 75 isolated compounds along with 34 previously reported xanthones were tested also for neuroprotective effects against the 1-methyl-4-phenylpyridinium ion (MPP+) and oxygen glucose deprivation (OGD)-induced cell death. Three compounds were active against MPPtinduced cell death with, EC50 values of 0.2-10.3 mu M, and 23 compounds were active in the OGD model with EC50 values of 2.9-35.5 mu M.</P>
Cytotoxic Drimane Sesquiterpenoids Isolated from <i>Perenniporia maackiae</i>
Kwon, Jaeyoung,Lee, Hyaemin,Seo, Young Hye,Yun, Jieun,Lee, Jun,Kwon, Hak Cheol,Guo, Yuanqiang,Kang, Jong Soon,Kim, Jae-Jin,Lee, Dongho American Chemical Society and American Society of 2018 Journal of natural products Vol.81 No.6
<P>A chemical investigation of a basidiomycetes fungus, <I>Perenniporia maackiae</I>, led to the discovery of 12 drimane sesquiterpenoids, including seven new constituents (<B>1</B>-<B>7</B>). The elucidation of the structures was performed via interpreting extensive spectroscopic methods, including ECD calculations. Among all isolated compounds, <B>1</B>, <B>2</B>, and <B>6</B> exhibited cytotoxicity toward six carcinoma cells, including ACHN, HCT-15, MDA-MB-231, NCI-H23, NUGC-3, and PC-3 cells, with half-maximal inhibition of cell proliferation values of 1.2-6.0 μM.</P> [FIG OMISSION]</BR>
<i>Xylodon flaviporus</i>-Derived Drimane Sesquiterpenoids That Inhibit Osteoclast Differentiation
Kwon, Jaeyoung,Lee, Hyaemin,Ryu, Seung Mok,Jang, Yeongseon,Kwon, Hak Cheol,Guo, Yuanqiang,Kang, Jong Soon,Kim, Jae-Jin,Lee, Dongho American Chemical Society and American Society of 2019 Journal of natural products Vol.82 No.10
<P>The presence of excessive osteoclasts is a major factor in skeletal diseases. The present study aimed to discover osteoclast differentiation inhibitors from the basidiomycete <I>Xylodon flaviporus</I>. Seven new drimane sesquiterpenoids (<B>1</B>-<B>7</B>) and 7-ketoisodrimenin-5-ene (<B>8</B>) were obtained and characterized by various spectroscopic methods. The isolated compounds were evaluated for their inhibitory effects against receptor activator of nuclear factor-kappa-B ligand-induced osteoclastogenesis in mouse bone marrow macrophages. Compounds <B>1</B>, <B>3</B>, and <B>6</B> showed potent activities with IC<SUB>50</SUB> values of 1.6, 0.9, and 2.1 μM, respectively, while <B>4</B>, <B>5</B>, and <B>7</B> exhibited relatively weak activities with IC<SUB>50</SUB> values of 10.7, 10.1, and 8.5 μM, respectively.</P> [FIG OMISSION]</BR>
Kwon, Jaeyoung,Lee, Hyaemin,Yoon, Yeo Dae,Hwang, Bang Yeon,Guo, Yuanqiang,Kang, Jong Soon,Kim, Jae-Jin,Lee, Dongho American Chemical Society and American Society of 2016 Journal of natural products Vol.79 No.6
<P>Two new spiro-lanostane triterpenoids, antrolactones A and B (1 and 2), along with polyporenic acid C (3), were isolated from an EtOAc-soluble extract of Antrodia heteromorpha culture medium, and the chemical structures of the new compounds were elucidated by application of NMR, MS, and ECD spectroscopic techniques. All isolated compounds exhibited inhibitory effects on receptor activator of nuclear factor-kappaB ligand-induced osteoclastogenesis.</P>
Neuroprotective Xanthones from the Root Bark of <i>Cudrania tricuspidata</i>
Kwon, Jaeyoung,Hiep, Nguyen Tuan,Kim, Dong-Woo,Hwang, Bang Yeon,Lee, Hak Ju,Mar, Woongchon,Lee, Dongho American Chemical Society and American Society of 2014 Journal of natural products Vol.77 No.8
<P>Seventeen new prenylated xanthones (<B>1</B>–<B>17</B>) were isolated from an ethyl acetate-soluble extract of root bark of <I>Cudrania tricuspidata</I> together with 17 previously identified xanthones. The structures of the new compounds were elucidated by spectroscopic methods. Six new compounds (<B>3</B>, <B>7</B>, <B>8</B>, <B>9</B>, <B>15</B>, and <B>16</B>) and six known compounds (<B>18</B>–<B>23</B>) showed neuroprotective effects against 6-hydroxydopamine-induced cell death in human neuroblastoma SH-SY5Y cells, with EC<SUB>50</SUB> values of 0.7–16.6 μM.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jnprdf/2014/jnprdf.2014.77.issue-8/np500364x/production/images/medium/np-2014-00364x_0003.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/np500364x'>ACS Electronic Supporting Info</A></P>
( Yujin Kwon ),( Su-yeon Cho ),( Jaeyoung Kwon ),( Min Hwang ),( Hoseong Hwang ),( Yoon Jin Kang ),( Hyeon-seong Lee ),( Jiyoon Kim ),( Won Kyu Kim ) 생화학분자생물학회 2022 BMB Reports Vol.55 No.6
The treatment of atopic dermatitis (AD) is challenging due to its complex etiology. From epidermal disruption to chronic inflammation, various cells and inflammatory pathways contribute to the progression of AD. As with immunosuppressants, general inhibition of inflammatory pathways can be effective, but this approach is not suitable for long-term treatment due to its side effects. This study aimed to identify a plant extract (PE) with anti-inflammatory effects on multiple cell types involved in AD development and provide relevant mechanistic evidence. Degranulation was measured in RBL-2H3 cells to screen 30 PEs native to South Korea. To investigate the anti-inflammatory effects of Parasenecio auriculatus var. matsumurana Nakai extract (PAE) in AD, production of cytokines and nitric oxide, activation status of FcεRI and TLR4 signaling, cell-cell junction, and cell viability were evaluated using qRT-PCR, western blotting, confocal microscopy, Griess system, and an MTT assay in RBL-2H3, HEK293, RAW264.7, and HaCaT cells. For in vivo experiments, a DNCBinduced AD mouse model was constructed, and hematoxylin and eosin, periodic acid-Schiff, toluidine blue, and F4/80-staining were performed. The chemical constituents of PAE were analyzed by HPLC-MS. By measuring the anti-degranulation effects of 30 PEs in RBL-2H3 cells, we found that Paeonia lactiflora Pall., PA, and Rehmannia glutinosa (Gaertn.) Libosch. ex Steud. show an inhibitory activity of more than 50%. Of these, PAE most dramatically and consistently suppressed cytokine expression, including IL-4, IL-9, IL-13, and TNF-α. PAE potently inhibited FcεRI signaling, which mechanistically supports its basophil-stabilizing effects, and PAE downregulated cytokines and NO production in macrophages via perturbation of toll-like receptor signaling. Moreover, PAE suppressed cytokine production in keratinocytes and upregulated the expression of tight junction molecules ZO-1 and occludin. In a DNCB-induced AD mouse model, the topical application of PAE significantly improved atopic index scores, immune cell infiltration, cytokine expression, abnormal activation of signaling molecules in FcεRI and TLR signaling, and damaged skin structure compared with dexamethasone. The anti-inflammatory effect of PAE was mainly due to integerrimine. Our findings suggest that PAE could potently inhibit multi-inflammatory cells involved in AD development, synergistically block the propagation of inflammatory responses, and thus alleviate AD symptoms. [BMB Reports 2022; 55(6): 275-280]
Usefulness of the Cytomegalovirus Antigenemia Assay in Patients With Ulcerative Colitis
( Jaeyoung Chun ),( Changhyun Lee ),( Ji Eun Kwon ),( Sung Wook Hwang ),( Sang Gyun Kim ),( Joo Sung Kim ),( Hyun Chae Jung ),( Jong Pil Im ) 대한장연구학회 2015 Intestinal Research Vol.13 No.1
Background/Aims: Patients with ulcerative colitis (UC) are at high risk for cytomegalovirus (CMV) reactivation. The useful-ness of the CMV antigenemia assay in active UC patients has rarely been studied. We assessed whether the assay detects CMV colitis and predicts clinical outcomes in patients with UC. Methods: We retrospectively reviewed the medical records of pa-tients hospitalized for moderate-to-severe UC from 2003 to 2012. Positive CMV antigenemia was defined as ≥1 pp65-positive cell per 2×105 polymorphonuclear neutrophils. CMV colitis was defined as the presence of inclusion bodies and/or positive im-munohistochemistry in the colonic mucosa. The primary outcome was steroid refractoriness, defined as the absence of clinical improvement after intravenous high-dose steroid administration. Results: A total of 43 patients were enrolled. CMV antigen-emia was detected in 12 (27.9%) patients. Positive CMV antigenemia was significantly associated with CMV colitis (P=0.001). The sensitivity and specificity of positive CMV antigenemia for diagnosing CMV colitis were 66.7% and 87.1%, respectively. Steroid refractoriness was found in 11 of 12 (91.7%) and 12 of 31 (38.7%) patients with positive and negative CMV antigenemia, respectively (P=0.002). The independent predictors for steroid refractoriness were positive CMV antigenemia (adjusted odds ratio [OR], 7.73; 95% confidence interval [CI], 1.22-49.19; P=0.030) and a shorter duration from the diagnosis of UC (adjusted OR, 0.99; 95% CI, 0.98-0.99; P=0.025). Conclusions: The CMV antigenemia assay shows low sensitivity but high specificity for detecting CMV colitis and may predict steroid-refractory UC. Early rescue therapy might be considered in UC patients positive for CMV antigenemia. (Intest Res 2015;13:50-59)