Purification and Characterization of Helicobacter pylori ${\gamma}$-Glutamyltranspeptidase

Song, Jae-Young,Choi, Yeo-Jeong,Kim, Jeong-Min,Kim, Yoo-Ree,Jo, Jin-Seong,Park, Jin-Sik,Park, Hee-Jin,Song, Yun-Gyu,Lee, Kon-Ho,Kang, Hyung-Lyun,Baik, Seung-Chul,Youn, Hee-Shang,Cho, Myung-Je,Rhee, Kw The Korean Society for Microbiology 2011 Journal of Bacteriology and Virology Vol.41 No.4

Gamma-glutamyltranspeptidase (GGT) was purified to electrophoretic homogeneity from the cell extract of H. pylori. The purified enzyme consisted of heavy and light subunits with molecular weights of 38 kDa and 21 kDa, respectively. N-terminal amino acid sequence of heavy and light subunits revealed that H. pylori GGT was processed into 3 parts for a signal peptide of 27 amino acid residues, a heavy subunit of 352 residues, and a light subunit of 188 residues during translation. The reaction rate for hydrolysis of ${\gamma}$-GpNA was 84.4 ${\mu}mol/min$ per milligram of protein, and that for the ${\gamma}$-glutamyl transfer from ${\gamma}$-GpNA to gly-gly was 23.8 ${\mu}mol/min$ per milligram of protein. The apparent Km values of H. pylori GGT for ${\gamma}$-glutamyl compounds were on the order of $10^{-3}$ to $10^{-4}$ M and those for acceptor peptides and amino acids were on the order of $10^{-1}$ to $10^{-2}$ M. The GGT protein kept approximately 80% of the initial enzymatic activity on incubation at $60^{\circ}C$ for 15 min. The optimum temperature and pH for reactions of both hydrolysis and transpeptidation were $40^{\circ}C$ and 9.0, respectively. The transpeptidation and hydrolysis reactions catalyzed by H. pylori GGT were strongly inhibited by L-Gln and moderately inhibited by L-Ala, L-Ser, ${\beta}$-chloro-L-Ala, and L-Glu. These results demonstrated that the biochemical properties of H. pylori GGT are different from those of other bacterial GGTs. Further, H. pylori GGT might degrade glutathione in the gastric mucous layer of humans if the enzyme could be secreted in the bacterial niches.

왜소음경증과 남성 성기능에 관한 연구

송윤섭,김민의,이남규,박영호,전윤수,김응구 순천향의학연구소 1997 Journal of Soonchunhyang Medical Science Vol.3 No.2

Micropenis is defined as a normally formed penis with a size greater than 2 standard deviations below the mean. Twenty three patients with micropenis were managed at Soonchunhyang University Hospital from January 1988 to December 1995 We interviewed and examined them with micropenis to investigate the cause of micropenis, concomitant diseases, the efficacy of treatment and their sexual activity in postpubertal men. The patients were divided into 2 groups: 16 prepubertal and 7 postpubertal men. 9 patients had cryptorchidism, 3 of them were bilateral and 5 patients had hypospadias. Etiology was determined by hormonal assay including HCG stimulation test ; 8 as hypogonadotrophic hypogonadism, 4 as primary testicular dysfunction and 5 as idiopathic. However etiology was undetermined in 6 patients. All patients had endocrine therapy using HCG or depot testosterone. All except 4 cases responded satisfactorily. All postpubertal patients(group 2) were below the 10th percentile. Puberty was delayed in 3 patients in the older group. All patients were heterosexual and they have had erections and orgasms. Five patients have had ejaculations, 3 of them were sexually active and they have experienced vaginal penetration, Nevertheless, their sexual partners have had complaints and the patients themselves have felt ashamed and dissatisfied. Fortunately, they were mentally healthy. None of these patients were married or had a child.

BCR-ABL 융합유전자를 동반한 급성 골수구성 백혈병 1예

최정민,송성은,이규진,이규원,이호섭 고신대학교 의과대학 2009 고신대학교 의과대학 학술지 Vol.24 No.1

BCR-ABL 융합 유전자를 동반한 급성골수구성백혈병은 그 빈도가 매우 낮고, 만성골수구성백혈병의 급성악화기와 임상적으로 유사한 특징을 가진다. 항암화학요법으로 관해유도가 되기 어렵거나, 관해유도 후 재발의 빈도가 높은 특징을 가지고 있어 예후가 나쁘다고 알려져있다. 저자들은 BCR-ABL 융합 유전자를 동반한 급성골 수구성백혈병1례를 경험하였기에 문헌고찰과 함께 보고 하는 바이다. BCR-ABL fusion gene is the hallmark of chronic myelogenous leukemia (CML), but it has been rarely reported in acute myeloid leukemia (AML). AML with BCR-ABL fusion gene has different features from blast crisis of CML. We experienced a rare case of AML with BCR-ABL fusion gene. Here we report this case with a brief review of literatures.

인터넷 게시판 답글의 동시적 특성

송민규 고려대학교 한국학연구소 2004 한국학연구 Vol.21 No.-

인터넷 게시판과 대화방은 동시성의 여부에 따라 서로 다른 언어 사용 양상을 보인다. 게시판은 문어적 특성이 많이 드러나는데 이는 게시판의 비등시적 특성 때문이다. 그러나 게시판의 일부인 답글은 동시적 특성을 갖는 경우가 있는데 이러한 통시적 특성이 게시판 자체의 정보전달력을 크게 하는 원인이 된다. 답글은 크게 댓글과 덧글로 이루어져 있으며 댓글은 게시판 목록에 새로운 게시물을 올리는 형식으로 사용되고 덧글은 특정 게시물의 본문아래에 덧붙이는 형식으로 사용된다. 댓글보다는 덧글에서 동시적 특성이 더 잘 드러나며 덧글에서 구어체의 사용이 빈번하다. 등수놀이와 리플놀이 등 게시판에서 크게 유행하고 있는 놀이들은 덧글이 동시성을 지니고 있다는 것을 보여주는 증거이며 도배와 악플은 등시성에 의해 형성된 이야기의 맥락을 끊는 요소로 작용한다. 인터넷 게시판 답글의 동시성은 인터넷 게시판에게 부족했던 양방향 커뮤니케이션을 가능하게 하여 게시판의 상호작용성을 높이는 결과를 가져온다. Internet BBS(Bulletin Board system) language is different from Chatting language from a synchronicity of view. BBS language has written language property because of it's non-synchronicity. But BBS Response Message has a synchronicity that vest it in BBS. Response Message is made of Reply and Comment. Reply is analogous with BBS language in it's expression. In Comment, spoken language property is observed frequently, it is an evidence of synchronicity. A Comment Ranking Play and Reply Play are popular in Netizen, these are the origin of synchronicity. Copy and Paste repeatedly and Evil Response disturb synchronous communication. A synchronicity of Internet BBS Response Message vests Two-way Directionality in BBS and improves BBS's Interactivity.

농촌지역 노인들의 우울증상에 관련된 요인들의 특성

송미숙,전기홍,송현종,김정은,박승구,신상건,이동훈,정성현,최연주,최완철,최민규,황재철,홍지만 아주대학교 1997 아주의학 Vol.2 No.2

This study was performed to identify risk factors for depression among the elderly in a rural area. A survey was done for the elderly living in Gosam-myenn, Ansung-kun during the period of July 28, 1997 and July 30, 1997. Demographic and sodoeconornic characteristics, and functional and health Status were assessed using a structured questionnaire, and the level of depression was measured by GDS score. The data obtained were analyzed by frequencies, percentage, t-test, ANOVA, and multiple regression, and the results were as follows: 1. The total sample consisted of 133 elderly. Of them, 47 elderly (35.4%) showed significant depressive Symptoms. 2. The GDS score was the highest for the group over 85 years of age (p < 0.05). Those living alone or widowed showed lower GDS score than those living with someone or married (p < 0.05). 3. The GDS score was the highest in the absolutely dependent group, followed by the partially dependent group, and the independent group (p < 0.01). The elderly who perceived their health Status as excellent showed the lowest GDS score. In contrast, those who had more than five chronic diseases showed the highest GDS score. 4. The elderly receiving financial and emotional support from their family or friends showed lower GDS score compared to those without any support. 5. The economically dependent group showed higher GDS score than that of independent group. 6. The elderly who involved in leisure activities showed lower GDS score than those without any involvement. 7. In terms of risk factors for depression, we found that perceived health Status, subjective economic Status, leisure activities, living arrangement and financial support were inversely associated with GDS score, whereas labor support was positively associated with GDS score. Based upon the above findings, this study suggests that these risk factors for depression in the elderly should be taken into consideration for a comprehensive mental health program for the elderly living in the rural area.

글루코오스의 분해반응에 미치는 금속이온의 영향

이민규,허목,이송우,송승구 濟州大學校工科大學附屬産業技術硏究所 1991 尖端技術硏究所論文集 Vol.2 No.-

The objective of this research was to study the chemical kinetics of the decomposition of glucose in the presence of acid catalyst and metal ion. In this experiment, the range of variables investigated was covered temperature from 160℃ to 180℃ and concentration of 4% to 11%(W/V) sulfuric acid and metal sulfate concentrations, ?? to ??g㏖/l. It is shown that glucose disappearance follows a first-order reaction. mechanism and the glucose decomposition reaction is dependent on both the reaction temperature and the catalyst concentration. And the presence of chromic ion accelerates the decomposition reaction of glucose, but presence of ferric and nickel ion rarely influence the reaction.

목재셀룰로오스의 산촉매 가수분해 반응속도론

이민규,송승구 제주대학교 1991 논문집 Vol.33 No.-

목재셀룰로오스의 가수분해에서 세가지 반응속도모델을 검토한 결과, 가수분해 반응은 A(셀룰로오스)→B(글루코오스)→C(분해물질)의 비가역 연속 1차반응으로 근사됨을 보여주었으며, 황산농도 4∼12%, 반응온도 170∼190℃에서 구한 반응속도 상수는 다음과 같았다. 셀룰로오스의 가수분해(A→B): k₁=6.6x10¹??(S)¹??exp(-39,500/RT) 글루코오스의 분해(B→A): k₂=6.4x10¹??(S)¹??¹exp(-30,800/RT) 반응속도식 결과에 의하면 당생성 수율 및 당농도는 반응온도 및 산농도가 증가함에 따라 증가하였으나. 그에 따른 반응시간의 감축으로 공정 취급상의 어려움을 안고 있었다. The experimental analysis of batch hydrolysis of wood cellulose at moderate sulfuric acid concentration showed that the hydorlysis of wood cellulose could be modeled by consecutive irreversible first order reactions. The kinetic parameters were evaluated covering the conditions of 170 to 190℃ and 4.4∼12.2% sulfuric acid. Hydrolysis of cellulose: k₁=6.6X10¹??(S)¹??exp(-39,500/RT) Decomposition of glucose: k₂=6.4X10¹??(S)¹??¹exp(-30,800/RT) Results showed that the sugar yield and concentration would increase with reaction temperature and acid concentration.

글루코오스의 분해반응에 미치는 금속이온의 영향

이민규,이송우,허목,송승구 濟州大學校 工科大學 産業技術硏究所 1991 産業技術硏究報告 Vol.2 No.-

The objective of this research was to study the chemical kinetics of the decompo-sition of glucose in the presence of acid catalyst and metal ion. In this experiment. the range of variables investigated was covered temperature from 160℃ to 180℃ and concentration of 4% to 11% (W/V) sulfuric acid and metal sulfate concentrations, 1.0×10^(-3) to 2.5×10^(-2) gmol/l. It is shown that glucose disappearance follows a first-order reaction mechanism and the glucose decomposition reaction is dependent on both the reaction temperature and the catalyst concentration. And the presence of chromic ion accelerates the decompo-sition reaction of glucose, but presence of ferric and nickel ion rarely influence the reaction.

치매환자를 위한 어플리케이션에 대한 연구

이철민(Lee Chul?min),임병규(Lim Boung-gyu),송은지(Song Eun-jee) 한국정보기술학회 2018 Proceedings of KIIT Conference Vol.2018 No.11

One-Chip uP에 의한 CO₂가스메탈 아크용접기의 디지털 제어기 설계에 관한 연구

최규하,채영민,송성학,우동학,한광수,안기은 건국대학교 1997 學術誌 Vol.41 No.2

With the development of semiconductor devices, adaptation of microcontroller prevails in industrial area. But in arc welding industry, there has not been much progress in adoption microcontroller technology. Therefore, this paper shows the application of digital control technology to welding machine. This paper presents the design of microcontroller that is appropriate for three phase CO2 gas metal arc welding machine. By using microcontroller in CO2 gas metal arc welding machine, the overact cost can be reduced compared to analog type one. Also TMS370C850 microprocessor is used as overall microcontroller for the arc welding machine.