Complete chloroplast genome sequences of Solanum commersonii and its application to chloroplast genotype in somatic hybrids with Solanum tuberosum

Cho, K. S.,Cheon, K. S.,Hong, S. Y.,Cho, J. H.,Im, J. S.,Mekapogu, M.,Yu, Y. S.,Park, T. H. Springer International 2016 Plant cell reports Vol. No.

<P>Chloroplast genome of Solanum commersonii and S olanum tuberosum were completely sequenced, and Indel markers were successfully applied to distinguish chlorotypes demonstrating the chloroplast genome was randomly distributed during protoplast fusion. Somatic hybridization has been widely employed for the introgression of resistance to several diseases from wild Solanum species to overcome sexual barriers in potato breeding. Solanum commersonii is a major resource used as a parent line in somatic hybridization to improve bacterial wilt resistance in interspecies transfer to cultivated potato (S. tuberosum). Here, we sequenced the complete chloroplast genomes of Lz3.2 (S. commersonii) and S. tuberosum (PT56), which were used to develop fusion products, then compared them with those of five members of the Solanaceae family, S. tuberosum, Capsicum annum, S. lycopersicum, S. bulbocastanum and S. nigrum and Coffea arabica as an out-group. We then developed Indel markers for application in chloroplast genotyping. The complete chloroplast genome of Lz3.2 is composed of 155,525 bp, which is larger than the PT56 genome with 155,296 bp. Gene content, order and orientation of the S. commersonii chloroplast genome were highly conserved with those of other Solanaceae species, and the phylogenetic tree revealed that S. commersonii is located within the same node of S. tuberosum. However, sequence alignment revealed nine Indels between S. commersonii and S. tuberosum in their chloroplast genomes, allowing two Indel markers to be developed. The markers could distinguish the two species and were successfully applied to chloroplast genotyping (chlorotype) in somatic hybrids and their progenies. The results obtained in this study confirmed the random distribution of the chloroplast genome during protoplast fusion and its maternal inheritance and can be applied to select proper plastid genotypes in potato breeding program.</P>

유리系 內의 Ferrite의 Mossbauer Spectra

安振盛,金俊植,李敬行,趙壽烈,董徹,洪致裕 동국대학교 자연과학연구소 1984 자연과학연구 논문집 Vol.4 No.-

本 硏究는 5NiO-5CdO-10Fe₂O₃-30Na₂CO₃-10SiO₂(mole %)유리계에 대한 Mossbauer parameter의 溫度依存性을 조사하였다. Mossbauer spectrum은 한쌍의 Quadrupole doublet의 중첩으로 fitting하였으며 이중 하나는 tetrahedral site,다른 하나는 octahedral site이다. 상대적인 共鳴吸收面積比로부터 octahedral site와 tetrahedral site에 대한 Debye temperature를 계산하여 본 결과 각각 418K, 449K 임을 알았다. 또한 θ?값들을 이용해 least square method로 isomer shiftdral site의 壓力依存系數 α와 2.97×10?mm/sec K, I.S(0)일때의 를 평가해본 결과 octahedral site의 α는 2.97×10?mm/sec K,I.S(0)는 tetrahedral site이고 α의 3.2305×10?mm/sec K, I.S(0)는 0.276mm/sec임을 알았다. 그리고 quadrupole splitting은 측정온도가 증가함에 따라 서서히 감소함을 알았다. The temperature dependence of the Mossbauer parameter for 5NiO-5CdO-10Fe₂O₃-30Na₂CO₃-10SiO₂(mole %) quenched glass system was investigated. The spectra were fitted as superposition of a pair of quadrupole double ; One is for the tetrahedral site and the other is for the octahedral site. From the relative absorption area ratios of the spectra, the Debye erature of two sites were calculated to be 418K and 449K for the octahedral site and the ?edrai site, respectively. From these Debye temperatures, We can see that the isomer shifts and the coefficients αof the pressure dependence of the isomer shift are 0.456mm/sec, 2.97×10?mm/sec K for octahedral site and 0.276mm/sec K, 3.2305×10?mm/sec K for ?edral site. Also, we can see that the quadrupole splittings of two sites are gradually decresed the increase of the measuring temperature.

Comparison of 90‐day case‐fatality after ischemic stroke between two different stroke outcome registries using propensity score matching analysis

Yu, K‐,H.,Hong, K‐,S.,Lee, B‐,C.,Oh, M‐,S.,Cho, Y‐,J.,Koo, J‐,S.,Park, J‐,M.,Bae, H‐,J.,Han, M‐,K.,Ju, Y‐,S.,Kang, D‐,W.,Appelros, P. Blackwell Publishing Ltd 2011 Acta neurologica Scandinavica Vol.123 No.5

<P>Yu K‐H, Hong K‐S, Lee B‐C, Oh M‐S, Cho Y‐J, Koo J‐S, Park J‐M, Bae H‐J, Han M‐K, Ju Y‐S, Kang D‐W, Appelros P, Norrving B, Terent A. Comparison of 90‐day case‐fatality after ischemic stroke between two different stroke outcome registries using propensity score matching analysis. 
Acta Neurol Scand: 2011: 123: 325–331. 
© 2010 John Wiley & Sons A/S.</P><P><B>Background – </B> It has not been clarified whether the disparity in ischemic stroke outcome between populations is caused by ethnic and geographic differences or by variations in case mix. Propensity score matching (PSM) analysis can overcome some analytical problems but is rarely used in stroke outcome research. This study was to compare the ischemic stroke case‐fatality between two PSM cohorts of Sweden and Korea.</P><P><B>Methods – </B> Prognostic variables related to baseline characteristics and stroke care were included in our PSM model. Then, we selected 7675 Swedish and 1220 Korean patients with ischemic stroke from each stroke registers and performed one‐to‐one matching based on propensity scores of each patient.</P><P><B>Results – </B> After PSM, all measured variables were well balanced in 1163 matched subjects, and the 90‐day case‐fatality was identical 6.2% (HR 0.997, 95%CI 0.905–1.099) in Sweden and Korea.</P><P><B>Conclusions – </B> No difference is found in the 90‐day case‐fatality in propensity score‐matched Swedish and Korean patients with ischemic stroke.</P>

Solubility of oxcarbazepine in eight solvents within the temperature range T=(288.15-308.15)K

Nam, K.,Ha, E.S.,Kim, J.S.,Kuk, D.H.,Ha, D.H.,Kim, M.S.,Cho, C.W.,Hwang, S.J. Academic Press 2017 The Journal of chemical thermodynamics Vol.104 No.-

In this study, the solubility of oxcarbazepine in pure methanol, ethanol, 1-propanol, 2-propanol, 1-butanol, acetone, acetonitrile, and tetrahydrofuran was analysed across the temperature range of 288.15-308.15K under atmospheric pressure by using a solid-liquid equilibrium method. The experimental values obtained data were correlated using the modified Apelblat model at each temperature. The mole fraction solubility of oxcarbazepine in all eight pure solvents increased gradually in a temperature-dependent manner. The highest mole fraction solubility of 3.08x10<SUP>-3</SUP> at 308.15K was observed for tetrahydrofuran, followed by acetone (1.82x10<SUP>-3</SUP> at 308.15K), acetonitrile (1.22x10<SUP>-3</SUP> at 308.15K), methanol (1.11x10<SUP>-3</SUP> at 308.15K), ethanol (6.17x10<SUP>-4</SUP> at 308.15K), 1-butanol (6.17x10<SUP>-4</SUP> at 308.15K), 1-propanol (6.16x10<SUP>-4</SUP> at 308.15K), and 2-propanol (4.13x10<SUP>-4</SUP> at 308.15K). The experimental solubility in all solvents correlated well with that calculated using the modified Apelblat equation across the temperature range of (288.15-308.15)K. Therefore, the experimental solubility and correlation equations established in this study could be useful during the crystallization/purification, pre-formulation, and formulation stages of oxcarbazepine production in laboratories and related industries.

한국인 Long Term Non Progressors로부터 분리된 HIV-1 env 및 nef 유전자와 이들의 면역 유전학적 특성 분석에 의한 질병진전 요인 규명

이주실,강춘,김성순,박근용,고운영,기미경,최병선,남정구,서순덕,이성래,구본기,박혜경,도경미,김태규,최희백,조현일,김요숙,김지영,이해정,안수진 국립보건연구원 1999 국립보건원보 Vol.36 No.-

Relationship between Stress Gene Polymorphisms and Litter Size by AI in Pigs

Jin, H.J.,Kim, I.C.,Wee, M.S.,Yeon, S.H.,Kim, C.D.,Lee, S.S.,Cho, C.Y.,Cho, S.R.,Son, D.S.,Park, C.K. 韓國受精卵移植學會 2007 한국동물생명공학회지 Vol.22 No.4

This study was performed to investigate the relationship between PSS-HSP70 gene polymorphism and artificial insemination (AI) reproductivity in the pigs. The RFLP polymorphism of PSS and the SSCP polymorphisms of HSP70 K1, K3 and K4 PCR product were detected different patterns. In the experiment for AI of fresh semen, spring and fall season showed higher litter size born of 10.89 head than 10.47 head of summer season. Landrace was showed higher litter size of 9.96 head than that of Duroc and Yorkshire (p<0.05). Stress relating PSS and HSP70 polymorphism of PSS-Normal, HSP70 K1-BB, K3-AB, K4-AA showd a highest litter size born of 10.97 head and litter size born alive of 10.69 head than that of the other polymorphisms(p<0.05). In the experiment for AI of frozen semen, effects of season and pig breeds were not showed for litter size born. The stress relating polymorphism of PSS-Carrier, HSP70 K1-BB, K3-BB, K4-AB showed highest litter size born of 11.29 head and litter size born alive of 10.82 head and PSS-Normal, HSP70 K1-BB, K3-AB, K4-AA showed the lowest litter size born of 8.48 head and litter size born alive of 7.33 head than that of the other polymorphisms(p<0.05). These results suggest that AI litter size born for the stress of forzen thawed semen may be affected by PSS and HSP70 polymorphism in pigs.

Facile synthesis of an IRMOF-3 membrane on porous Al₂O₃ substrate via a sonochemical route

Lee, Y.R.,Cho, S.M.,Ahn, W.S.,Lee, C.H.,Lee, K.H.,Cho, W.S. Elsevier 2015 Microporous and mesoporous materials Vol.213 No.-

A densely-packed IRMOF-3 membrane (S-IRMOF-3(Mem)), ~55 μm in thickness, on an Al<SUB>2</SUB>O<SUB>3</SUB> disc support was prepared via a sonochemical synthesis route, and the optimal fabrication conditions were established. Bare IRMOF-3 particles (S-IRMOF-3(p)) were synthesized independently to analyze the contribution of the Al<SUB>2</SUB>O<SUB>3</SUB> support. The overall physicochemical properties of the IRMOF-3 products produced via the sonochemical route were superior to those prepared by microwave heating. The IRMOF-3 materials produced were characterized by XRD, SEM, EDS-mapping, and N<SUB>2</SUB> adsorption-desorption measurements. S-IRMOF-3(p) exhibited satisfactory CO<SUB>2</SUB> adsorption capacities (54 mg/g at 298 K/1 bar; 732 mg/g at 298 K/20 bar) and CO<SUB>2</SUB>/N<SUB>2</SUB> selectivity (18 at 298 K/1 bar). Both S-IRMOF-3(Mem) and S-IRMOF-3(p) were assessed as a catalyst for a liquid phase Knoevenagel condensation reaction between benzaldehyde and ethyl cyanoacetate. S-IRMOF-3(Mem) and S-IRMOF-3(p) showed comparable conversions (87-89%) with 100% selectivity after a 4 h reaction at 333 K. The heterogeneous nature of the catalyst was confirmed by a hot filtering experiment, and S-IRMOF-3(Mem) could be recovered easily after the reaction and be recycled several times with little change in product yield.

Relationship between Stress Gene Polymorphisms and Litter Size by AI in Pigs

H. J. Jin,I. C. Kim,M. S. Wee,S. H. Yeon,C. D. Kim,S. S. Lee,C. Y. Cho,S. R. Cho,D. S. Son,C. K. Park,Z. D. Li 사단법인 한국동물생명공학회 2007 한국동물생명공학회지 Vol.22 No.4

This study was performed to investigate the relationship between PSS-HSP70 gene polymorphism and artificial insemination (AI) reproductivity in the pigs. The RFLP polymorphism of PSS and the SSCP polymorphisms of HSP70 K1, K3 and K4 PCR product were detected different patterns. In the experiment for AI of fresh semen, spring and fall season showed higher litter size born of 10.89 head than 10.47 head of summer season. Landrace was showed higher litter size of 9.96 head than that of Duroc and Yorkshire(p<0.05). Stress relating PSS and HSP70 polymorphism of PSS-Normal, HSP70 K1-BB, K3-AB, K4-AA showd a highest litter size born of 10.97 head and litter size born alive of 10.69 head than that of the other polymorphisms(p<0.05). In the experiment for AI of frozen semen, effects of season and pig breeds were not showed for litter size born. The stress relating polymorphism of PSS-Carrier, HSP70 K1-BB, K3-BB, K4-AB showed highest litter size born of 11.29 head and litter size born alive of 10.82 head and PSS-Normal, HSP70 K1-BB, K3-AB, K4-AA showed the lowest litter size born of 8.48 head and litter size born alive of 7.33 head than that of the other polymorphisms(p<0.05). These results suggest that AI litter size born for the stress of forzen thawed semen may be affected by PSS and HSP70 polymorphism in pigs.

세관 양광주 방전에서 플라즈마 확산의 완전 해

김동준,정종문,김정현,황하청,정재윤,조윤희,임현교,구제환,최은하,조광섭,Jin, D.J.,Jeong, J.M.,Kim, J.H.,Hwang, H.C.,Chung, J.Y.,Cho, Y.H.,Lim, H.K.,Koo, J.H.,Choi, E.H.,Cho, G.S. 한국진공학회 2010 Applied Science and Convergence Technology Vol.19 No.1

관경이 수 mm인 세관 램프 내부에서 플라즈마의 확산을 조사하기 위하여 이극성(ambipolar) 확산방정식을 해하였다. 반경 방향의 확산에 의한 유리관 벽에서의 플라즈마 소멸 특성시간은 $\tau_r\;=\;(r_0/2.4)^2/D_a$로 주어진다. 반경 $r_0{\sim}1\;mm$이고 이극성 확산계수 $D_a{\sim}0.01\;m^2/s$ 이면, $\tau_r{\sim}17\;{\mu}s$이다. 이는 램프의 교류전원 구동에서 플라즈마를 유지하기 위한 구동 최소 주파수 ~30 kHz에 해당한다. 고전압이 인가되는 전극부에 발생한 고밀도의 플라즈마가 양광주로 확산되는 특성시간은 $\tau_z{\sim}0.1\;s$이다. 고밀도 플라즈마 경계에서의 시간에 대한 확산속도는 $t{\sim}10^{-6}\;s$일 때 $u_D{\sim}10^2\;m/s$이고, $t{\sim}10^{-3}\;s$이면 그 속도는 $u_D{\sim}1\;m/s$로 느려진다. 따라서 램프 길이 ~1 m에 대하여 전극부에서 생성된 고밀도 플라즈마가 양광주 전체로 확산되는 시간은 수 초가 걸린다. The ambipolar diffusion equation has been solved in a fine-tube lamp of a few mm in diameter. In the diffusion of radial direction, the plasma diffuses and vanishes away at the glass wall by recombination with the characteristic time of plasma loss is given by $\tau_r\;=\;(r_0/2.4)^2/D_a$. With the radius $r_0{\sim}1\;mm$ and the ambipolar diffusion coefficient $D_a{\sim}0.01\;m^2/s$, the vanishing time is calculated $\tau_r{\sim}10\;{\mu}s$ which corresponds to the least value of frequency 30 kHz for the sustaining the plasma in the operation of high voltage AC-power. In the diffusion of longitudinal z-direction, a high density plasma generated at the area of a high voltage electrode, diffuses into the positive column with the characteristic time $\tau_z{\sim}0.1\;s$. The plasma diffusion velocity at the boundary of high density plasma is $u_D{\sim}10^2\;m/s$ at the time $t{\sim}10^{-6}$ s and the diffusion velocity becomes slow as $u_D{\sim}1\;m/s$ at $t{\sim}10^{-3}\;s$. Therefore, for the long lamp of 1 m, it takes about several seconds for the high density plasma at the area of electrode to diffuse through the whole positive column space.

燐酸 및 加里肥料가 葉煙草의 品質에 미치는 影響 : Nicotine의 含量에 對하여 On the Contents of Nicotine in Tobacco leaves

趙成鎭,禹相圭,金才正,卞珠燮 충북대학교 엽연초연구소 1971 煙草硏究 Vol.1 No.-

This experiment was carried out to determine the effect of different levels of applied fertilizer quality of leaf-tobacco. It was done at all varying levels of phosphate and potassium fertilizer; S_0 (non-fertilizer plot) ; N:P:K=0:0:0(Kg/10α) S₁ (half an amount plot) ; N:P:K=10:7.5:10(〃) S₂ (Standard plot) ; N:P:K=10:15:20(〃) S₃ (1.5 times plot) ; N:P:K=10:22.5:30(〃) S₄ (2.0times plot) ; N:P:K=10:30:40(〃) Urea was applied as nitrogen, double susperphosrhate of lime as phosphate, potassium sulfate as potash source. The tonal amount of fertilizer was applied as basal dressing per one plant. This experiment was carried out by using a randomized block design with 3 replication. The results obtained in terms of quality are summarized as follows. (1) The Content of P₂O_5, and K₂O in tobacco leaf increased while that of nicotine in tobacco leaf decreased by increasing the amount of phosphate and potassium fertilizer in soil. Negative correlation between them was observed. (2) It seems that increased phosphate and potassium fertilizer levels made boron absorption in soil increase, thereby Nicotine contents decrease. Negative correlation between them was observed (3) S₁ plot showed potassium deficiency. It could be explained on the basis of N/K₂O ratio. It was considered that sufficient amount of phosphate and potassium fertilizer will be needed to ensure the enhancement quality of tobacco leaf.