Isolation and enzymatic characterization of the first reported hyaluronidase from Yak (Bos grunniens) testis

Ru-ren Li,Qun-li Yu,Ling Han,Liang-yan Rong,Meng-meng Yang,Mai-rui An 한국화학공학회 2014 Korean Journal of Chemical Engineering Vol.31 No.11

A novel hyaluronidase (BgHya1) from Yak testis was isolated and shown to have compara-tively high activity on sodium hyaluronate. However, surveys on BgHya1 are still limited. The enzyme was purifiedthrough gel filtration on Sephacryl S-100 and cation-exchange on SP Sepharose fast flow; the purity was confirmedby a reverse phase FPLC Shodex C4 column. The specific activity of the purified BgHya1 was 20.4 U/mg assayed bythe colorimetric method against 0.85 U/mg for the crude enzyme, representing a 24-fold purification. It was a monomericprotein of 55 kDa estimated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and SephacrylS-200. It exhibited maximum activity in the presence of 0.15 M NaCl at 37 oC, pH 3.8, and a specificity to sodiumhyaluronate higher than that of chondroitin-4-sulfate, chondroitin-6-sulfate, and dermatan. The Km value for BgHya1,using sodium hyaluronate as substrate, was 0.106 mg/mL. Activity of BgHya1 was inhibited mildly by Ca2+and Fe2+,and significantly by Fe3+, Mg2+, EDTA, urea, heparin, and 0.5 M NaCl. It was not affected by Cu2+,Zn2+,Co2+, ascorbicacid, PMSF, DTT, glutathione (reduced), or L-cysteine. BgHya1 was shown to be heat unstable in the range of 4-45 oC. In terms of storage stability, 92% of the activity was retained after four weeks at 4 oC, and 58% at room temperature. In addition, adding BSA (1.0 mg/mL) to the enzyme sample prior to freezing resulted in complete retention of enzymeactivity. This work yielded a high purity hyaluronidase, the first one isolated from by-product.

Nutritional Characteristics and Active Components in Liver from Wagyu×Qinchuan Cattle

Ru-Ren Li,Ling Han,Hui Cao 한국축산식품학회 2014 한국축산식품학회지 Vol.34 No.2

We investigated nutritional characteristics and active components in the liver of Wagyu×Qinchuan cattle and Qinchuancattle produced in Shaanxi (China). We observed significant differences (p<0.05) in the proximate composition of protein,fat, carbohydrate, total energy, and glycogen. Wagyu×Qinchuan cattle liver showed higher (p<0.05) sodium, iron, zinc, andselenium concentrations than Qinchuan cattle liver. The amino acid composition of Wagyu×Qinchuan cattle liver was richer(p<0.05) in 13 types of amino acids, with the exception of Asp (10.06%), Val (5.86%), and Met (1.72%). Total essentialamino acids accounted for almost half the composition (39.69%) in Wagyu×Qinchuan cattle liver. Wagyu×Qinchuan cattleliver had lower (p<0.05) levels of monounsaturated fatty acids (18.2%), but higher (p<0.05) levels of polyunsaturated fattyacids (35.11%), compared with Qinchuan cattle liver (23.29% and 28.11%, respectively). The thrombogenic index washigher in Qinchuan cattle liver (0.86) than in Wagyu×Qinchuan cattle liver (0.70), and the glutathione (38.0 mg/100g) andL-carnitine (2.12 µM/g) content was higher (p<0.05) in Wagyu×Qinchuan cattle liver than in Qinchuan cattle liver(29.8 mg/100g and 1.41 µM/g, respectively). According to the results obtained, the liver of Wagyu×Qinchuan cattle, whichis insufficiently used, should be increasingly utilized to improve its commercial value.

Nutritional Characteristics and Active Components in Liver from Wagyu×Qinchuan Cattle

Li, Ru-Ren,Yu, Qun-Li,Han, Ling,Cao, Hui Korean Society for Food Science of Animal Resource 2014 한국축산식품학회지 Vol.34 No.2

We investigated nutritional characteristics and active components in the liver of Wagyu${\times}$Qinchuan cattle and Qinchuan cattle produced in Shaanxi (China). We observed significant differences (p<0.05) in the proximate composition of protein, fat, carbohydrate, total energy, and glycogen. Wagyu${\times}$Qinchuan cattle liver showed higher (p<0.05) sodium, iron, zinc, and selenium concentrations than Qinchuan cattle liver. The amino acid composition of Wagyu${\times}$Qinchuan cattle liver was richer (p<0.05) in 13 types of amino acids, with the exception of Asp (10.06%), Val (5.86%), and Met (1.72%). Total essential amino acids accounted for almost half the composition (39.69%) in Wagyu${\times}$Qinchuan cattle liver. Wagyu${\times}$Qinchuan cattle liver had lower (p<0.05) levels of monounsaturated fatty acids (18.2%), but higher (p<0.05) levels of polyunsaturated fatty acids (35.11%), compared with Qinchuan cattle liver (23.29% and 28.11%, respectively). The thrombogenic index was higher in Qinchuan cattle liver (0.86) than in Wagyu${\times}$Qinchuan cattle liver (0.70), and the glutathione (38.0 mg/100g) and L-carnitine (2.12 ${\mu}M/g$) content was higher (p<0.05) in Wagyu${\times}$Qinchuan cattle liver than in Qinchuan cattle liver (29.8 mg/100g and 1.41 ${\mu}M/g$, respectively). According to the results obtained, the liver of Wagyu${\times}$Qinchuan cattle, which is insufficiently used, should be increasingly utilized to improve its commercial value.

Market Reactions to the Split-share Structure Reform and the Determinants of Compensation: Evidence from Chinese Listed Firms

Li Cheng,Jeng-Ren Chiou,Yenn-Ru Chen,LEEBONGSOO 한국증권학회 2012 Asia-Pacific Journal of Financial Studies Vol.41 No.2

The split-share structure is a unique characteristic of corporate ownership in China, and is often linked to poor firm performance and inefficient corporate governance. In this paper, we investigate market reactions around several important event days during the process of the split-share structure reform (share reform) in Chinese listed firms. The market reacts differently to different events during the process. First, the market reacts positively to firms’ intention of implementing the share reform when the reform prospectus is disclosed and when the compensation is actually paid. Second, after the reform is actually implemented, the market reacts negatively when the expiration date of the lockup agreement is announced, because the restricted trading shares can be freely traded and thus share supply increases after the expiration date. In addition, the compensation paid to tradable shareholders by non-tradable shareholders is determined by the stock liquidity before the reform, the proportion of restricted trading shares, and the type of ultimate shareholders. We further find that the payment of compensation, the proportion of restricted shares after the share reform, and a time gap between suspension and resumption days are the main factors affecting the market reaction.

The Antibiosis Action and Rice-Induced Resistance, Mediated by a Lipopeptide from Bacillus amyloliquefaciens B014, in Controlling Rice Disease Caused by Xanthomonas oryzae pv. oryzae

( Shu Bin Li ),( Shi Ru Xu ),( Rui Ning Zhang ),( Yuan Liu ),( Ren Chao Zhou ) 한국미생물 · 생명공학회 2016 Journal of microbiology and biotechnology Vol.26 No.4

In the present study, a lipopeptide (named AXLP14) antagonistic to Xanthomonas oryzae pv. oryzae (Xoo) was obtained from the culture supernatant of Bacillus amyloliquefaciens B014. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analysis demonstrated that AXLP14 consisted of surfactin homologs. The minimum inhibition concentration and minimum bactericidal concentration of AXLP14 against Xoo were determined to be 1.25 and 2.50 mg/ml, respectively. At a concentration of 0.613 mg/ml, AXLP14 strongly inhibited the formation of Xoo biofilm. AXLP14 also inhibited the motility of Xoo in a concentration-dependent manner. Applying AXLP14 to rice seedlings significantly reduced the incidence and severity of disease caused by Xoo. In Xoo-infected rice seedlings, AXLP14 strongly and continuously up-regulated the expression of both OsNPR1 and OsWRKY45. In addition, AXLP14 effectively inhibited the Xoo-induced up-regulation of the expression of the abscisic acid biosynthesis gene OsNECD3 and the abscisic acid signalingresponsive gene OsLip9, indicating that AXLP14 may protect rice against Xoo-induced disease by enhancing salicylic acid defense and interfering with the abscisic acid response to virulence.

Phase separation phenomena and thermodynamics of octylphenol polyoxyethylene ether in micellar solutions: Effect of high concentration of inorganic salts and linear correlation

Dan Ni Li,Jing Huang,Zhao Hua Ren,Rui Sheng,Zheng Bo Qian,Bei Bei Li,Xiao Feng Quan,Ye Xi Zhang,Jun Ru Wang,Huan Tian 한국공업화학회 2021 Journal of Industrial and Engineering Chemistry Vol.99 No.-

The phase separationphenomena or cloud points (CP) behaviorof nonionic octylphenol polyoxyethylene etherwith oxyethylene glycol ether unit of 15 (OP-15) were investigated in aqueous micellar solutions containinghigh concentrations of inorganic salts including NaCl, KCl, MgCl2, CaCl2, BaCl2, AlCl3, FeCl3, Na2SO4and Na2SiO3. The change in CP induced by salts and its mechanism are discussed theoretically. In aqueous solutionscontaining high concentration of salt, for thefirst time the linear relationship between CP and saltconcentrationiswellestablishedtocharacterizethedepressionofCP.Basedonpseudophaseseparationmodel,thermodynamic parameters are estimated and adopted to further describe the phase separation process andthe interactions between OP-15 molecules or the OP-15/water interactions. Thermodynamic parametersincluding standard Gibbs energy change (DG0CP), enthalpy change (DH0CP) and entropy change (DS0CP)indicate that the additions of different salts result in the change in entropy-driven nonspontaneousprocess of phase separation. These data, which are from the compensation temperatures and the changeof molar heat capacity, also prove that different ions induce the change in the interactions betweensurfactant molecules or the surfactant–water interaction and thus affect the depression of CP. Thesefindings help with understanding the effect of inorganic salts, especially the high concentration of salt, onCP of nonionic surfactant and then broadening the application of nonionic surfactant based on CP such asthe extraction of biomaterials in biological samples derived from animals or plants, etc.

Research Articles : Off-Road Machinery System Engineering ; Road-friendliness of Fuzzy Hybrid Control Strategy Based on Hardware-in-the-Loop Simulations

( Tian Yi Yan ),( Qiang Li ),( Kun Ru Ren ),( Yu Lin Wang ),( Lu Zou Zhang ) 한국농업기계학회 2012 바이오시스템공학 Vol.37 No.3

Purpose: In order to improve road-friendliness of heavy vehicles, a fuzzy hybrid control strategy consisting of a hybrid control strategy and a fuzzy logic control module is proposed. The performance of the proposed strategy should be effectively evaluated using a hardware-in-the-loop (HIL) simulation model of a semi-active suspension system based on the fuzzy hybrid control strategy prior to real vehicle implementations. Methods: A hardware-in-the-loop (HIL) simulation system was synthesized by utilizing a self-developed electronic control unit (ECU), a PCI-1711 multi-functional data acquisition board as well as the previously developed quarter-car simulation model. Road-friendliness of a semi-active suspension system controlled by the proposed control strategy was simulated via the HIL system using Dynamic Load Coefficient (DLC) and Dynamic Load Stress Factor (DLSF) criteria. Results: Compared to a passive suspension, a semi-active suspension system based on the fuzzy hybrid control strategy reduced the DLC and DLSF values. Conclusions: The proposed control strategy of semi-active suspension systems can be employed to improve road-friendliness of road vehicles.

Road-friendliness of Fuzzy Hybrid Control Strategy Based on Hardware-in-the-Loop Simulations

Yan, Tian Yi,Li, Qiang,Ren, Kun Ru,Wang, Yu Lin,Zhang, Lu Zou Korean Society for Agricultural Machinery 2012 바이오시스템공학 Vol.37 No.3

Purpose: In order to improve road-friendliness of heavy vehicles, a fuzzy hybrid control strategy consisting of a hybrid control strategy and a fuzzy logic control module is proposed. The performance of the proposed strategy should be effectively evaluated using a hardware-in-the-loop (HIL) simulation model of a semi-active suspension system based on the fuzzy hybrid control strategy prior to real vehicle implementations. Methods: A hardware-in-the-loop (HIL) simulation system was synthesized by utilizing a self-developed electronic control unit (ECU), a PCI-1711 multi-functional data acquisition board as well as the previously developed quarter-car simulation model. Road-friendliness of a semi-active suspension system controlled by the proposed control strategy was simulated via the HIL system using Dynamic Load Coefficient (DLC) and Dynamic Load Stress Factor (DLSF) criteria. Results: Compared to a passive suspension, a semi-active suspension system based on the fuzzy hybrid control strategy reduced the DLC and DLSF values. Conclusions: The proposed control strategy of semi-active suspension systems can be employed to improve road-friendliness of road vehicles.

Degradation of Lignocelluloses in Rice Straw by BMC-9, a Composite Microbial System

( Hongyan Zhao ),( Hai Ru Yu ),( Xu Feng Yuan ),( Ren Zhe Piao ),( Hu Lin Li ),( Xiao Fen Wang ),( Zong Jun Cui ) 한국미생물 · 생명공학회 2014 Journal of microbiology and biotechnology Vol.24 No.5

To evaluate the potential utility of pretreatment of raw biomass with a complex microbial system, we investigated the degradation of rice straw by BMC-9, a lignocellulose decomposition strain obtained from a biogas slurry compost environment. The degradation characteristics and corresponding changes in the bacterial community were assessed. The results showed that rapid degradation occurred from day 0 to day 9, with a peak total biomass bacterium concentration of 3.3 × 10(8) copies/ml on day 1. The pH of the fermentation broth declined initially and then increased, and the mass of rice straw decreased steadily. The highest concentrations of volatile fatty acid contents (0.291 mg/l lactic acid, 0.31 mg/l formic acid, 1.93 mg/l acetic acid, and 0.73 mg/l propionic acid) as well as the highest xylanse activity (1.79 U/ml) and carboxymethyl cellulase activity (0.37 U/ml) occurred on day 9. The greatest diversity among the microbial community also occurred on day 9, with the presence of bacteria belonging to Clostridium sp., Bacillus sp., and Geobacillus sp. Together, our results indicate that BMC-9 has a strong ability to rapidly degrade the lignocelluloses of rice straw under relatively inexpensive conditions, and the optimum fermentation time is 9 days.

Analysis of Rock Mass Parameters and Plastic Zone of a Tunnel in Southwest Mountainous Area

Mengni Wang,Zhilong Zhang,Li Ren,Ru Zhang,Liangjuan Zheng,Lanbin Zhang,Zetian Zhang,Jing Xie,Xiaoling Liu 대한토목학회 2023 KSCE Journal of Civil Engineering Vol.27 No.12

The deformation damage and support difficulties caused by the formation and expansion of plastic zone of surrounding rock have always been urgent engineering problems to be solved, especially in areas with prominent non-uniform stress fields. Choosing a reasonable and practical method to determine the plastic zone is of great significance for ensuring the long-term stability and safety of tunnels. Based on a tunnel project in the southwest mountainous area, this paper conducted mechanical tests to obtain the mechanical parameters of granite. The analytical method optimized based on the principle of stress balance was applied, and the numerical simulation was carried out according to the field displacement monitoring data and experimental parameters, the plastic zone of the medium quality granite cross-section and fault fracture zone granite cross-section was analyzed comprehensively. The results shown that the analytical and numerical solutions are in good agreement with engineering practice. It proved that the theory and method in this paper are scientific and practical in engineering. The research results can provide support for the design optimization and construction safety of the tunnel, and the research ideas and methods can also provide reference for similar projects.