RAW264.7 세포에서 interferon-r 및 LPS에 의해 유도되는 NO생성에 미치는 TALT-35의 영향

박종일,박경석,김종석,박지훈,윤은진,송경섭,서강식,김훈,윤완희,박승길,임규,황병두 충남대학교 생물공학연구소 2006 생물공학연구지 Vol.12 No.-

TALP-35 purified from human term placenta is known to increase microtubule polymerization and stabilize the polymerized microtubule. To examine the effect of TALP-35 on immune system this study was performed. MTT assay was performed to investigate the effect of TALP-35 on the proliferation of RAW264.7 cells. TALP-35 dose dependently suppress the proliferation of RAW264.7 cells at high concentration (above 1 μM) in unstimulated cells, in case of 10 μM TALP-35 treated cells the suppression was 25% but in stimulated cells it was only 15%. Cosedimentation assay was carried out to investigate whether TALP-35 can bind to tubulin of RAW264.7, monocyte/macrophage lineage of mouse, and polymerize it. TALP-35 polymerize the tubulin of RAW264.7 cells and sedimented in dose-dependent manner. To investigate the effect of TALP-35 on the expression of iNOS protein western blotting was performed. The expression level of iNOS was decreased dose dependently in high concentration of TALP-35 treatment. To examine the activity of iNOS, secreted NO was determined by method based on Griess reaction. Interferon-γ and LPS-stimulated production of NO from RAW264.7 cells was decreased dose dependently above 0.1 μM concentration of TALP-35 and 50% is decreased at 10μM of it. This study shows TALP-35 can control cytokine induced-iNOS expression therefore it might control inflammatory diseases.

슬관절 골관절염 증상이 환자에게 미치는 우울 효과

박영규,이윤영,이계우,김정아,윤도경,박승회,조경환,홍명호,박용규 대한노인병학회 2001 대한노인병학회지 Vol.5 No.4

The effect of neuropeptides on secretion of Interleukin-8(IL-8)

Kim, Kyung-Jun,Park, Sang-Hyuk,Choi, Kyoung-Kyu,Park, Sang-Jin 大韓齒科保存學會 2006 Restorative Dentistry & Endodontics Vol.31 No.3

본 연구는 치수조직, 치은, 치주인대로부터 배양된 조직을 SP (Substance P)로 4시간, SP, CGRP (Calcitonin Gene-related Peptide), Tumor necrosis factor-α (TNF-α)로 8시간 자극 후 RNase Protection Assay를 시행하고, IL-8의 분비량을 측정해 다음 결과를 얻었다. 1. IL-8 mRNA는 모든세포에서 발현됐다. 2. IL-8 mRNA 발현은 SP (10^(-5)M)와 SP (10^(-8)M)로 4시간 자극 시 증가되지 않았다. 3. IL-8 mRNA 발현은 SP (10^(-4)M)와 CGRP (10^(-6)M)로 8시간 자극 시 증가되지 않았다. 4. TNF-α (2 ng/㎖) 자극 시, IL-8 mRNA 발현이 증가됐다. 5. 치은 세포를 CGRP (10^(-6)M)로 8시간 자극 시, IL-8 분비량이 증가했다 (p < 0.05). 6. 치주인대 세포를 SP (10^(-4)M)로 8시간 자극 시 IL-8 분비량이 증가했다 (p < 0.05). We investigated the secretion of Interleukin-8 (IL-8) from ginviva and periodontal ligament stimulated with Substance P (SP) and Calcitonin Gene-related Peptide (CGRP). Gingiva (GF), periodontal ligament (PDLF) and pulp (PF) tissues were collected from extracted instact 3rd molars. Cultured cells were stimulated with different concentrations of SP for 4 hrs, and stimulated with SP, CGRP and Tumor Necrosis Factor-α (TNF-α) for 8 hrs. Then RNase Protection Assay was carried out. ELISA was performed using supernatants of stimulated cells for quantitative analysis of IL-8. Results were assessed using supernatants of stimulated cells for quantitative analysis of IL-8. Results were assessed using student t-test with significance of P < 0.05. According to this study, the results were as follows: 1. IL-8 mRNA was detected in all type of cells studied (PF, GF and PDLF). 2. IL-8 mRNA expression was not increased after stimulating 4 hrs with SP (10^(-5)M) and SP (10^(-8)M) compared with Mock stimulation in all type of cells studied. 3. IL-8 mRNA expression was not increased after stimulating 8 hrs with SP (10^(-4)M) and CGRP (10^(-6)M) compared with Mock stimulation in all type of cells studied. 4. TNF-α (2 ng/㎖) increased the expression of IL-8 mRNA in all kind of cells studied. 5. The secretion of IL-8 from GF was increased 8 hrs after the stimulation with CGRP (10^(-6)M) (p < 0.05). 6. The secretion of IL-8 from PDLF was increased 8 hrs after the stimulation with SP (10^(-4)M) (p < 0.05). Calcitonin Gene-related Peptide (CGRP) increased Interleukin-8 (IL-8) which plays an important role in chemotaxis of neutrophil in Calcitonin Gene-related Peptide (CGRP) gingival tissue, whereas Substance P increased the secretion of IL-8 from periodontal ligament.

축구선수의 Detraining과 Retraining이 혈청지질 및 호르몬농도에 미치는 영향

정정화,박재현,채종훈,성혜련,황지인,윤미숙,노금선,윤종관,윤영학,노순덕,정경숙,박일규,김은희,박현태,박상갑 대한스포츠의학회 1999 대한스포츠의학회지 Vol.17 No.1

The purpose of this study was to investigate the effects of detraining and retraining on serum lipid and hormones in soccer players. Subjects were seven male high-school soccer players. V˙O_2max was determined for each subjects by administering a treadmill test(initial speed: 90m/min, grade: 5%, increasing speed per 3 min: 30m/min). Serum lipid(T-C, TG, HDL-C LDL-C) and hormones(epinephrine, norepinephrine, growth hormones, cortisol) were assayed pre and post detraining in 10, 20, 30 days after retraining. The repeated ANOVA was used to determine significant differences. The 0.05 level of significance was as critical level for the study. The results of the study were as follows: 1. V˙O_2max(ml/min) were 3576.3±204.2ml/min pre detraining, 3234.1±198.9 ml/min post detraining. There are significant(p<.05) difference between pre and post detraining. In 10, 20, 30 days after retraining, V˙O_2max(ml/min) were 3601.4±170.9 ml/min. There were significantly(p<.05) increased in retraining periods. 2. V˙O_2max(ml/kg/min) were significantly(p<.05) decreased from 62.3±2.9 ml/kg/min to 55.9±4.7 ml/kg/min in detraining. In 10, 20, 30 days after retraining, V˙O_2max(ml/kg/min) were 62.4±3.4ml/kg/min, 62.7±2.3ml.kg/min, 67.3±7.2ml/kg/min respectively. There were significantly(p<.05) increased in retraining periods. 3. T-C were significantly (p<.05) increased from 166.6±8.5mg/dl to 175.3±10.3 mg/dl in detraining. In 10, 20, 30 days after retraining, T-C were 160.1± 3.2mg/dl, 156.7±3.7mg/dl, 140.3±9.0mg/dl. There were significantly(p<.05) decreased in retraining periods. 4. HDL-C were 61.4±6.6mg/di pre detraining, 5.3±6.6mg/dl post detraining. There are significant(p<.05) difference between pre and post detraining. In 10, 20, 30 days after retraining, HDL-C were 56.9±7.1mg/dl, 56.4±9.2mg/dl, 57.7±9.1mg/dl respectively. There were no significant difference in retraining periods. 5. The hormones(epinephrine. norepinephrine, growth hormone, cortisol) were changed as same patterns. Epinephrine were 26.0±7.0[g/ml pre detraining, 24.6±3.2pg/ml post detraining. In 10, 20, 30 days after retraining, epinephrine were 26.9±5.6pg/ml, 30.6±6.2pg/ml, 29.4±5.6pg/ml respectively. There were no significant difference in retraining periods. In conclusion, HDL-C, epinephrine, norepinephrine, growth hormone and cortisol were decreased, T-C, LDL-C and TG were increased in detraining. But HDL-C, epinephrine, norepinephrine, growth hormone and cortisol were increased, T-C, LDL-C and TG were decreased in retraining.

슬관절 골관절염 환자에서 근육내 자극요법의 치료 효과

박영규,우종웅,안승렬,김정아,윤도경,박승회,조경환,홍명호 대한노인병학회 2001 대한노인병학회지 Vol.5 No.4

科學1의 學習指導와 評價의 標準化에 關한 硏究

朴載昊,鄭敬圭,鄭世泳 부산대학교 사범대학 과학교육연구소 1984 科學敎育硏究報 Vol.11 No.-

The model of evaluation of the sciencs lesson in the science section of junior high school was studied for the establishment of teaching method by the analysis of logical structure of the science lesson and learning practice. In teaching several detailed conditions were established by the judgement of learners exact readiness. It is shown in the survey of learning practice that although science teachers understand the essential of science they have some difficulties in the practical use of scientific knowledge. Several detailed conditions of evaluation of the science lesson were proposed and some items of evaluation of the science lesson were exemplified.

사회적 스트레스, 범죄 및 사고발생간의 인과적 모형에 관한 연구

박경구,임영규 계명대학교 사회과학연구소 2000 한국사회과학연구 Vol.19 No.2

This thesis aimed at identifying how social structure produces social stress, and how social stress leads to crime and accidents. More specifically, this study tested the hypothesis that the incidence of crime and accidents was influenced by the index of social structure and the degree of social stress. The data were collected from government statistical databases dated of December 31, 1995. The methods of LISREL were applied to analyze the data. The results were as follows: The index of social structure influenced over the incidence of crime and accidents directly and indirectly by way of provoking social stress. The estimated parameter of the effects of the social structure on crime is negative, but its indirect effects were estimated positive. The total effects were positive, implying that the social structure led the incidence of crime. The similar pattern of parameter estimation was found in the case of accidents. But the negative direct effects were larger than the positive indirect effects. It means that the social structure tended to reduced the incidence of accidents. To explain this result is not easy, but one possible alternative is that the large cities have better facilities for safety. The social stress itself exerted its impact on the incidence of crime and accidents. As the city experienced more stress, crime and accidents took place more frequently. Asl a conclusion, it could be said that the social structure induced the social stress, resulting crime and accidents.

화상성 액와부 구축반혼 교정술에 대한 연구

박�규,민경원,이윤호 大韓成形外科學會 1985 Archives of Plastic Surgery Vol.12 No.1

A retrograde study was performed in order to analyse and evaluate the operation results of 45 axillary burn scar contracture patients, who were treated in our hospital over last 5 years, in various view points. Some new operation techniques were tried out, such as fasciocutaneous bipedicled flap and latissimus dorsi myocutaneous flap, for the purpose of correcting the axillary contracture and the results revealed many advantages, especially in the severe cases which have not been able to be corrected effectively. Comparison between the results obtained by new tecniques and those by previous techniques led up to make up the scientific and schematic operation protocol for correction of axillary contracture. This paper presents not only the various advantages of fasciocutaneous bipedicled flap and latissimus dorsi myocutaneous flap, but also the new schematic operation protocol according to 5 graded classification of axillary burn scar contracture.

장기간 운동이 비만여성의 특성불안 및 관련 생리변인에 미치는 영향

박기용,박영명,박병규,김성진,최경훈 한국스포츠리서치 2004 한국 스포츠 리서치 Vol.15 No.3

The purpose of this study was to examine the changes of trait anxiety and its related physiological variables in obese women through chronic exercise. A total of twenty three college female students having over 30% of body at were equally assigned to an experimental group or a control group. The experimental group lasted aerobic exercise for 40 minutes a day, 3 times per week for 10 weeks, Their exercise intensity was 50% to 70% of VO₂ max, An independent t-test using each group's gain scores showed that changes of the trait anxiety(p<.05), body fat(p<.01), and VO₂ max(p<.05) were significantly different from those of the control group. There were, however, no significant differences between the two groups in the changes or the blood pressure and the recovery heart rates, It was concluded that chronic exercise can lower trait anxiety in obese women, However, the decreased trait anxiety seems to be independently associated with blood pressure changes.

대전에서 연중 태양광선의 UVA와 UVB양에 대한 조사 (예비보고)

박장규,김경훈,이우재,서기범 충남대학교 의과대학 지역사회의학연구소 1993 충남의대잡지 Vol.20 No.2

Ultraviolet A(355 nm) and ultraviolet B(290 nm) doses were measured at Taejon from March to September, 1993, on four different weather conditions(clear, relatively clear, relatively cloudy and cloudy). And the results were compared with the doses of Kongju area measured in 1985. The followings were the summary of the findings. 1. UVA(1.98± 0.35 × 10 exp(-3) w/㎠) and UVB(17.62+ 5.72 X 10 exp(-6) w/㎠ doses were peak at noon on clear weather. 2. Average doses of UVA(2.36±0.70 × exp(-3) w/㎠) and UVB(24.28±8.83 × exp(-3) w/㎠) were peak at noon in August on clear weather. 3. The doses of UVA and UVB were influenced by yellow sand, fog and other pollutants. 4. The doses of UVA on clear weather in Daejoen were lower than Kongju about 40%, in contrast those of UVB were not different in statistical values.