CYP2W1, CYP4F11 and CYP8A1 Polymorphisms and Interaction of CYP2W1 Genotypes with Risk Factors in Mexican Women with Breast Cancer

Cardenas-Rodriguez, N.,Lara-Padilla, E.,Bandala, C.,Lopez-Cruz, J.,Uscanga-Carmona, C.,Lucio-Monter, P.F.,Floriano-Sanchez, E. Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.3

Breast cancer (BCa) is the leading type of cancer in Mexican women. Genetic factors, such as single nucleotide polymorphisms (SNP) of P450 system, have been reported in BCa. In this report, and for the first time in the literature, we analyzed the rs3735684 (7021 G>A), rs11553651 (15016 G>T) and rs56195291 (60020 C>G) polymorphisms in the CYP2W1, 4F11 and 8A1 genes in patients with BCa and in healthy Mexican women to identify a potential association between these polymorphisms and BCa risk. Patients and controls were used for polymorphism analysis using an allelic discrimination assay with TaqMan probes and confirmed by DNA sequencing. Links with clinic-pathological characteristics were also analyzed. Statistical analysis was performed using the standard ${\chi}^2$ or Fisher exact test statistic. No significant differences were observed in the distributions of CYP2W1 (OR 8.6, 95%CI 0.43-172.5 P>0.05; OR 2.0, 95%CI 0.76-5.4, P>0.05) and CYP4F11 (OR 0.3, 95%CI 0.01-8.4 P>0.05) genotypes between the patients and controls. Only the CYP8A1 CC genotype was detected in patients with BCa and the controls. All polymorphism frequencies were in Hardy-Weinberg Equilibrium (HWE) in the controls (P>0.05). We found a significant association between BCa risk and smoking, use of oral contraceptives or hormonal replacement therapy (HRT), obesity, hyperglycemia, chronic diseases, family history of cancer and menopausal status in the population studied (P<0.05). Tobacco, oral contraceptive or HRT, chronic diseases and obesity or overweight were strongly associated with almost eight, thirty-five, nine and five-fold increased risk for BCa. Tobaco, obesity and hyperglycemia significantly increased the risk of BCa in the patients carrying variant genotypes of CYP2W1 (P<0.05). These results indicate that the CYP2W1 rs3735684, CYP4F11 rs11553651 and CYP8A1 rs56195291 SNPs are not a key risk factor for BCa in Mexican women. This study did not detect an association between the CYP2W1, 4F11 and 8A1 genes polymorphisms and BCa risk in a Mexican population. However, some clinico-pathological risk factors interact with CYP2W1 genotypes and modifies susceptibility to BCa.

Reproductive Response of Ewes Fed with Taiwan Grass Hay (Pennisetum purpureum Schum.) Supplemented with Duckweed (Lemna sp. and Spirodela sp.)

Zetina-Cordoba, P.,Ortega-Cerrilla, M.E.,Torres-Esqueda, M.T. Sanchez,Herrera-Haro, J.G.,Ortega-Jimenez, E.,Reta-Mendiola, J.L.,Vilaboa-Arroniz, J. Asian Australasian Association of Animal Productio 2012 Animal Bioscience Vol.25 No.8

The effect of duckweed (DW) supplementation was evaluated on dry matter intake (DMI), presence and duration of estrus, percentage of ewes repeating estrus and pregnancy rate, as well as the concentration of progesterone ($P_4$) in multiparous crossbred ewes from Pelibuey, Dorper, and Katahdin breeds, fed with Taiwan grass hay (TWH). Eighteen ewes with $39.7{\pm}4kg$ mean body weight, kept in individual pens, were randomly assigned to one of the following treatments: $T_1$: TWH, $T_2$: TWH plus 200 g DW, $T_3$: TWH plus 300 g DW. The ewes were synchronized with 40 mg fluorogestone acetate (FGA) and 400 UI equine chorionic gonadotropin (eCG). Data were analyzed as a completely randomized design using the GLM procedure. DW supplementation had no effect on dry matter intake (p>0.05); however, a slight decrease of TWH intake was observed as DW supplementation increased. No differences (p>0.05) were found in the beginning of estrus, percentage of ewes presenting it, its duration, or pregnancy rate. There were no differences (p>0.05) on $P_4$ concentration among treatments, or $treatment{\times}period$ interaction (p>0.05). However the period was significant (p<0.01), since the $P_4$ levels increased as time increased after the removal of the FGA device and eCG application.

A Call for a Rational Polypharmacy Policy: International Insights From Psychiatrists

Yukako Nakagami,Kohei Hayakawa,Toru Horinouchi,Victor Pereira-Sanchez,Marcus P,J,Tan,Seon-Cheol Park,Yong Chon Park,Seok Woo Moon,Tae Young Choi,Ajit Avasthi,Sandeep Grover,Roy Abraham Kallivayalil,Yu 대한신경정신의학회 2021 PSYCHIATRY INVESTIGATION Vol.18 No.11

Objective Recently, rational polypharmacy approaches have been proposed, regardless of the lower risk and cost of monotherapy. Considering monotherapy as first-line treatment and polypharmacy as rational treatment, a balanced attitude toward polypharmacy is recommended. However, the high prevalence of polypharmacy led the Japanese government to establish a polypharmacy reduction policy. Based on this, the association between the policy and psychiatrists’ attitude toward polypharmacy has been under debate. Methods We developed an original questionnaire about Psychiatrists’ attitudes toward polypharmacy (PAP). We compared the PAP scores with the treatment decision-making in clinical case vignettes. Multiple regression analyses were performed to quantify associations of explanatory variables including policy factors and PAP scores. The anonymous questionnaires were administered to psychiatrists worldwide. Results The study included 347 psychiatrists from 34 countries. Decision-making toward polypharmacy was associated with high PAP scores. Multiple regression analysis revealed that low PAP scores were associated with the policy factor (β=-0.20, p=0.004). The culture in Korea was associated with high PAP scores (β=0.34, p<0.001), whereas the culture in India and Nepal were associated with low scores (β=-0.15, p=0.01, and β=-0.17, p=0.006, respectively). Conclusion Policy on polypharmacy may influence psychiatrists’ decision-making. Thus, policies considering rational polypharmacy should be established.

Magnetic Resonance Myocardial Feature Tracking in Transfusion-Dependent Myelodysplastic Syndrome

Marta Alonso-Fernandez-Gatta,Ana Martin-Garcia,Maria Diez-Campelo,Agustin C. Martin-Garcia,Manuel Barreiro-Pérez,Félix Lopez-Cadenas,Elena Diaz-Pelaez,Pedro L. Sanchez 한국심초음파학회 2021 Journal of Cardiovascular Imaging (J Cardiovasc Im Vol.29 No.4

BACKGROUND: Myocardial deformation with echocardiography allows early detection of systolic dysfunction and is related to myocardial iron overload (MIO) determined by T2* in hereditary anemias under transfusion support. Our aim was to analyze the diagnostic and prognostic usefulness of magnetic resonance feature tracking (MR-FT) myocardial strain in low-risk myelodysplastic syndromes (LR-MDS) patients. METHODS: Prospective study in transfusion-dependent LR-MDS patients and healthy controls who underwent a cardiac MR-FT. We analyzed the relationships between strain MR-FT and iron overload parameters and its prognostic impact in cardiovascular events and/or death. RESULTS: Thirty-one patients and thirteen controls were included. MIO (T2* < 20 ms) was detected in 9.7% of patients. Left ventricular global longitudinal strain (LV-GLS) by MR-FT was pathological (> −19.3%) in 32.3% of patients. Less negative strain values correlated with lower T2* (R = −0.37, p = 0.033) and native myocardial T1 (R = −0.39, p = 0.031) times. LV-GLS by MR-FT was significantly associated with higher incidence of the combined cardiovascular events and/or all-cause death (p = 0.047), with a cut-off value of −17.7% for predicting them (63% sensitivity and 81% specificity, area under the curve = 0.69). After adjusting analysis including demographic, biomarkers and imaging variables, a higher LV-GLS value by MR-FT remained as predictor of combined event in transfusion-dependent LR-MDS patients (hazard ratio, 0.4; confidence interval, 0.15–0.98; p = 0.045). CONCLUSIONS: Longitudinal myocardial strain by MR-FT in LR-MDS patients is associated to MIO and correlates with adverse events in the follow-up, what could serve as a prognostic tool.

Design and experimental characterization of a novel passive magnetic levitating platform

E. Diez-Jimenez,R. Alcover-Sanchez,J.M. Soria,J. Pérez-Aracil,E. Pereira 국제구조공학회 2022 Smart Structures and Systems, An International Jou Vol.29 No.3

This work proposes a novel contactless vibration damping and thermal isolation tripod platform based on Superconducting Magnetic Levitation (SML). This prototype is suitable for cryogenic environments, where classical passive, semi active and active vibration isolation techniques may present tribological problems due to the low temperatures and/or cannot guarantee an enough thermal isolation. The levitating platform consists of a Superconducting Magnetic Levitation (SML) with inherent passive static stabilization. In addition, the use of Operational Modal Analysis (OMA) technique is proposed to characterize the transmissibility function from the baseplate to the platform. The OMA is based on the Stochastic Subspace Identification (SSI) by using the Expectation Maximization (EM) algorithm. This paper contributes to the use of SSI-EM for SML applications by proposing a step-by-step experimental methodology to process the measured data, which are obtained with different unknown excitations: ambient excitation and impulse excitation. Thus, the performance of SSI-EM for SML applications can be improved, providing a good estimation of the natural frequency and damping ratio without any controlled excitation, which is the main obstacle to use an experimental modal analysis in cryogenic environments. The dynamic response of the 510 g levitating platform has been characterized by means of OMA in a cryogenic, 77 K, and high vacuum, 1E-5 mbar, environment. The measured vertical and radial stiffness are 9872.4 N/m and 21329 N/m, respectively, whilst the measured vertical and radial damping values are 0.5278 Nm/s and 0.8938 Nm/s. The first natural frequency in vertical direction has been identified to be 27.39 Hz, whilst a value of 40.26 Hz was identified for the radial direction. The determined damping values for both modes are 0.46% and 0.53%, respectively.

Proton conducting sulfonated polysulfone and polyphenylsulfone multiblock copolymers with improved performances for fuel cell applications

Sydonne Swaby,Nieves Ureña,María Teresa Pérez-Prior,Carmen del Río,Alejandro Várez,Jean-Yves Sanchez,Cristina Iojoiu,Belén Levenfeld 한국공업화학회 2023 Journal of Industrial and Engineering Chemistry Vol.122 No.-

A series of proton exchange membranes based on sulfonated multiblock copolymers with three polysulfone(PSU) and polyphenylsulfone (PPSU) ratios (50/50, 60/40 and 75/25) are prepared following a synthesisstrategy that aims to achieve a microphase separation. A selective sulfonation of PSU blocks wasobserved in copolymers with a high proportion of PSU. The water uptake is higher in these materials(31% and 57% for SPES 50/50 and SPES 75/25, respectively at 60 C) while the tensile strength was lower(56.0 MPa and 40.6 MPa for SPES 50/50 and SPES 75/25 in the H+ form, respectively). Ionic conductivity ofSPES 75/25 membranes measured both ex situ and in situ at 80 C is 25 and 31 mScm1, respectively. Fuelcell tests reveal that SPES 75/25 shows the highest value for the maximum power density (670 mWcm2at 70 C and 100% of RH) which is higher than that achieved for SPES 50/50 (400 mWcm2). In addition,the high current density obtained for SPES 75/25 (1000 mAcm2 at 0.6 V and 70 C) compared withSPES50/50 (600 mAcm2) and Nafion 112 (450 mAcm2 at 75 C) shows its promising properties as solidelectrolyte in polymeric fuel cells.

Non-destructive estimation of leaf area and leaf weight of Cinchona officinalis L. (Rubiaceae) based on linear models

Huaccha-Castillo Annick Estefany,Fernandez-Zarate Franklin Hitler,Pérez-Delgado Luis Jhoseph,Tantalean-Osores Karla Saith,Vaca-Marquina Segundo Primitivo,Sanchez-Santillan Tito,Morales-Rojas Eli,Semin 한국산림과학회 2023 Forest Science And Technology Vol.19 No.1

Non-destructive methods that accurately estimate leaf area (LA) and leaf weight (LW) are simple and inexpensive, and represent powerful tools in the development of physiological and agronomic research. The objective of this research is to generate mathematical models for estimating the LA and LW of Cinchona officinalis leaves. A total of 220 leaves were collected from C. officinalis plants 10months after transplantation. Each leaf was measured for length, width, weight, and leaf area. Data for 80% of leaves were used to form the training set, and data for the remaining 20% were used as the validation set. The training set was used for model fit and choice, whereas the validation set al.lowed assessment of the of the model’s predictive ability. The LA and LW were modeled using seven linear regression models based on the length (L) and width (Wi) of leaves. In addition, the models were assessed based on calculation of the following statistics: goodness of fit (R2), root mean squared error (RMSE), Akaike’s information criterion (AIC), and the deviation between the regression line of the observed versus expected values and the reference line, determined by the area between these lines (ABL). For LA estimation, the model LA¼11.521(Wi) 21.422 (R2¼0.96, RMSE¼28.16, AIC¼3.48, and ABL¼140.34) was chosen, while for LW determination, LW¼0.2419(Wi) 0.4936 (R2¼0.93, RMSE¼0.56, AIC¼37.36, and ABL¼0.03) was selected. Finally, the LA and LW of C. officinalis could be estimated through linear regression involving leaf width, proving to be a simple and accurate tool.

A novel methodology for modal parameters identification of large smart structures using MUSIC, empirical wavelet transform, and Hilbert transform

Amezquita-Sanchez, Juan P.,Park, Hyo Seon,Adeli, Hojjat Elsevier 2017 ENGINEERING STRUCTURES Vol.147 No.-

<P><B>Abstract</B></P> <P>A key issue in health monitoring of smart structures is the estimation of modal parameters such as natural frequencies and damping ratios from acquired dynamic signals. In this article, a new methodology is presented for calculating the natural frequencies (NF) and damping ratios (DR) of large civil infrastructure from acquired dynamic signals using a multiple signal classification (MUSIC) algorithm, the empirical wavelet transform (EWT), and the Hilbert transform. The effectiveness of the proposed method is validated by means of three examples: a benchmark 3D 4-story steel frame structure, a benchmark problem, subjected to dynamic loading, an 8-story steel frame subjected to white noise input on a shaking table, and a 123-story highrise building structure, Lotte World Tower (LWT), under construction in Seoul, South Korea. The results demonstrate that the new methodology is accurate for estimating the NF and DR of a superhighrise building structure using low-amplitude ambient vibrations data, a complex and challenging task since the measured vibrations signals are noisy and present non-stationary characteristics. The new methodology can deal with noisy signals without degrading its ability to estimate the NF and DR of different one-of-a kind civil structures thus is particularly suitable for health monitoring of large smart structures under dynamic loading.</P> <P><B>Highlights</B></P> <P> <UL> <LI> A new methodology for modal parameter identification of large civil structures. </LI> <LI> It uses MUSIC-EWT algorithm and Hilbert transform. </LI> <LI> It is applied to a 123-story highrise building structure, Lotte World Tower. </LI> <LI> It is effective for modal parameter identification of superhighrise structures. </LI> <LI> It can deal with noisy signals. </LI> </UL> </P>

Final report on key comparison CCQM-K73: Amount content of H⁺ in hydrochloric acid (0.1 mol kg⁻¹)

Pratt, Kenneth W,Ortiz-Aparicio, Jose Luis,Matehuala-Sanchez, Francisco Javier,Pawlina, Monika,Kozlowski, Wladyslaw,Borges, Paulo P,da Silva Junior, Wiler B,Borinsky, Mó,nica B,Hernandez-Mabel P Springer-Verlag 2013 Metrologia Vol.50 No.-

<P>This key comparison (KC), CCQM-K73, was performed to demonstrate the capability of the participating National Metrology Institutes (NMIs) to measure the amount content of H<SUP>+</SUP>, ν<SUB>H<SUP>+</SUP></SUB>, in an HCl solution with a nominal ν<SUB>H<SUP>+</SUP></SUB> of 0.1 mol kg<SUP>−1</SUP>. The comparison was a joint activity of the Electrochemical Working Group (EAWG) and Inorganic Analysis Working Group (IAWG) of the CCQM and was coordinated by NIST (USA) and CENAM (Mexico).</P><P>The agreement of the results was not commensurate with the claimed uncertainties of the subset of participants that claimed small uncertainties for this determination. A workshop on technical issues relating to the CCQM-K73 measurements was conducted at the joint IAWG-EAWG meeting at the Bureau International des Poids et Mesures (BIPM), Paris (Sèvres) in April 2010. Several possible sources of bias were investigated, but none could explain the observed dispersion among the participants' results.</P><P>In the absence of a specific cause for the dispersion, the IAWG and EAWG decided to assign a Key Comparison Reference Value, KCRV, and standard uncertainty of the KCRV, u<SUB>KCRV</SUB>, based on the DerSimonian–Laird statistical estimator. The u<SUB>KCRV</SUB> is dominated by the between-laboratory scatter of results in CCQM-K73. The uncertainty estimates from the participants with the lowest reported uncertainties remain unsupported by this KC.</P><P>Main text.To reach the main text of this paper, click on Final Report. Note that this text is that which appears in Appendix B of the BIPM key comparison database kcdb.bipm.org/.</P><P>The final report has been peer-reviewed and approved for publication by the CCQM, according to the provisions of the CIPM Mutual Recognition Arrangement (CIPM MRA).</P>

Accumulation of coronary artery disease risk factors over three years: Data from an international inception cohort

Urowitz, M. B.,Gladman, D.,Ibañ,ez, D.,Fortin, P.,Sanchez-Guerrero, J.,Bae, S.,Clarke, A.,Bernatsky, S.,Gordon, C.,Hanly, J.,Wallace, D.,Isenberg, D.,Ginzler, E.,Merrill, J.,Alarcó,n, G. S Wiley Subscription Services, Inc., A Wiley Company 2008 Vol.59 No.2

<B>Objective</B><P>To examine the accumulation of risk factors over 3 years in a multicenter, international inception cohort of patients with systemic lupus erythematosus (SLE).</P><B>Methods</B><P>The Systemic Lupus International Collaborating Clinics registry for atherosclerosis comprises 27 centers from 11 countries. An inception cohort of 935 patients with SLE was assembled, according to a standardized protocol, from 2000 to 2006 to study risk factors for atherosclerosis. Both classic and other coronary artery disease (CAD) risk factors were collected at entry and through 3 years of followup. Therapy was documented over the 3 years. The Framingham 10-year risk factor profile was calculated for each patient at year 1 and year 3.</P><B>Results</B><P>A total of 278 patients from the inception cohort were followed for 3 years and constituted the population for this study. At enrollment a substantial number of patients already demonstrated several risk factors for CAD, both classic and other. All risk factors increased from enrollment over the 3 years of followup. Treatment of hypertension and hypercholesterolemia also increased over 3 years, but less so for hypercholesterolemia. The Framingham 10-year CAD risk profile was higher in men than in women both at entry and at 3 years, and remained unchanged over the 3 years. Corticosteroid use increased only slightly over 3 years, but use of antimalarials and immunosuppressive agents increased to a greater extent.</P><B>Conclusion</B><P>Patients with SLE should be monitored for CAD risk factors from the time of diagnosis and appropriate treatment should be instituted early.</P>