Epinephrine-Selective Electrode Based on Lipophilic 1,3-Bisbridged Cofacial-calix[6]crown-5-ether

Yeo, Hee-Kyoung,Lee, Hyo-Kyoung,Nam, Kye-Chun,Jeon, Seung-Won Korean Chemical Society 2004 Bulletin of the Korean Chemical Society Vol.25 No.3

The potentiometric response of electrode no. 4 based on 1,3-bisbridged cofacial-calix[6]crown-5-ether (IV) gave a sub-Nernstian (45.0 mV/decade) response and the best detection limit (-log $a_{ep}$ = 4.73) towards epinephrine. The responses are decreasing in the order of epinephrine > $K^+$, dopamine > $NH_4^+$ > norepinephrine > $Na^+$. It is remarkable that the proposed electrode shows the reasonable selectivity to epinephrine against other catecholamine neurotransmitters (dopamine and norepinephrine) as well as alkali metal ions.

Selective inhibition of V600E-mutant BRAF gene induces apoptosis in thyroid carcinoma cell lines

Kyoung Sik Park,Madhuri Saindane,Eun Yeol Yang,TongYi Jin,Harikrishna Reddy Rallabandi,Alexander Heil,Sang Eun Nam,Young Bum Yoo,Jung-Hyun Yang,Jong Bin Kim,Seo-Young Park,Won Seo Park,Yeo-Kyu Youn 대한외과학회 2021 Annals of Surgical Treatment and Research(ASRT) Vol.100 No.3

Purpose: Papillary thyroid cancer (PTC) has a high incidence of BRAF<SUP>V600E</SUP> mutation. The purpose of this study was to evaluate the potential relationship between thyroiditis and BRAF<SUP>V600E</SUP> mutation status in patients with PTC. We investigated how a selective inhibitor of BRAF<SUP>V600E</SUP> PLX4032 affects the proliferation and inflammatory cytokine levels of thyroid cancer. Methods: Two thyroid cancer cell lines TPC1 and 8505C were treated with PLX4032, an analysis was done on cell growth, cell cycle, the degree of apoptosis, and levels of inflammatory cytokines. To identify the functional links of BRAF, we used the STRING database. Results: Docking results illustrated PLX4032 blocked the kinase activity by exclusively binding on the serine/threonine kinase domain. STRING results indicated BRAF is functionally linked to mitogen-activated protein kinase. Both cell lines showed a dose-dependent reduction in growth rate but had a different half maximal inhibitory concentration value for PLX4032. The reaction to PLX4032 was more sensitive in the 8505C cells than in the TPC1 cells. PLX4032 induced a G2/M phase arrest in the TPC1 cells and G0/G1 in the 8505C cells. PLX4032 induced apoptosis only in the 8505C cells. With PLX4032, the TPC1 cells showed decreased levels of vascular endothelial growth factor, granulocyte-macrophage colony-stimulating factor, chemokine (C-C motif) ligand 2/monocyte chemoattractant protein 1, whereas the 8505C cells showed significantly decreased levels of IL-8, serpin E1/plasminogen activator inhibitor-1, and matrix metalloproteinase (MMP)-3. Conclusion: PLX4032 was cytotoxic in both TPC1 and 8505C cells and induced apoptosis. In the 8505C cells, inflammatory cytokines such as IL-8 and MMP-3 were down-regulated. These findings suggest the possibility that the BRAF<SUP>V600E</SUP> mutation needs to target inflammatory signaling pathways in the treatment of thyroid cancer.

Medium Optimization for Pediocin SA131 Production by Pediococcus pentosaceus SA131 against Bovine Mastitis Using Response Surface Methodology

Yeo Lang Park,Na Kyoung Lee,Keun Kyu Park,Yong Ho Park,Jong Man Kim,Hyang Mi Nam,Suk Chan Jung,Hyun Dong Paik 한국축산식품학회 2010 한국축산식품학회지 Vol.30 No.1

Pediococcus pentosaceus SA131 was isolated from jeotgal, is the bacteriocin producer against bovine mastitis pathogens, Streptococcus uberis E290, Enterococcus gallinarum E362, and Staphylococcus epidermidis ATCC 12228. The medium composition for pediocin SA131 production by P. pentosaceus SA131 was optimized using response surface methodology. Component of medium was studied as carbon source (glucose, fructose, lactose, glycerol, sucrose, maltose, and mannitol), nitrogen source (beef extract, yeast extract, peptone, malt extract, and tryptone), mineral and surfactant (MgSO4, KH2PO4, (NH4)2SO4, MnSO4, NaCl, sodium acetate, and Tween 80). Through one factor-at-a-time experiment, glucose, fructose, yeast extract, malt extract, NaCl, MgSO4, and Tween 80 were determined as the good ingredient. The effects of major factors for pediocin SA131 production were investigated by two-level fractional factorial designs (FFD). By a 2(4) FFD, fructose, yeast extract, and MnSO4 were found to be the important factors for the bacteriocin production. Subsequently, a 2(3) central composite design (CCD) was adopted to derive a statistical model for optimizing the composition of the fermentation medium. The estimated optimum composition for the production of pediocin SA131 by P. pentosaceus SA131 was as follows; 0.13% fructose, 1% glucose, 1.8% yeast extract, 2.58% MnSO4, 0.2% NaCl, and 0.2% Tween 80. The pediocin production under optimized medium was increased to 1,000 AU/mL, compared to the 400 AU/mL in MRS medium.

Risk of Tuberculosis Development in Patients with Rheumatoid Arthritis Receiving Targeted Therapy: a Prospective Single Center Cohort Study

Yeo-Jin Song,Soo-Kyung Cho,Hyoungyoung Kim,Hye Won Kim,Eunwoo Nam,Sang-Cheol Bae,Dae Hyun Yoo,Yoon-Kyoung Sung 대한의학회 2021 Journal of Korean medical science Vol.36 No.10

Background: Patients with rheumatoid arthritis (RA) undergoing targeted therapy have a higher risk of developing tuberculosis (TB). This requires diagnosis and treatment of latent tuberculosis infection (LTBI). We aimed to evaluate whether diagnosis and treatment of LTBI in RA are effective in Korea, and to estimate the risk of TB development by calculating the incidence rate of active TB among RA patients receiving targeted therapy. Methods: We analyzed data from two prospective cohort studies of RA patients who received biologic disease-modifying antirheumatic drugs (bDMARDs) or Janus kinase (JAK) inhibitor. We selected new starters of targeted therapy and classified them into three groups receiving tumor necrosis factor (TNF) inhibitor, non-TNF inhibitor, and JAK inhibitor, respectively. We then compared LTBI prevalence, treatments, and active TB incidence during first-line therapy in each group. Results: A total of 765 RA patients (574 TNF inhibitor users, 132 non-TNF inhibitor users, and 59 JAK inhibitor users) were included in this study. Observation periods were 1,255.2 person-years (PYs), 264.7 PYs, and 53.3 PYs, respectively. All 765 patients underwent LTBI screening, and the positivity rate was 26.5% (n = 203). Of the 203 LTBI-positive patients, 189 (93.1%) received treatment. Only one patient, who was in the TNF inhibitor group, and was negative for the interferon gamma release assay (IGRA), did not receive LTBI treatment and developed active TB during follow-up. Conclusion: Although the prevalence of LTBI in RA patients who started targeted therapy was slightly elevated, the Korean guidelines specifying LTBI screening and treatment were effective in preventing latent TB from becoming active.

신이식 환자에서 혈청 호모시스틴 농도의 변화

김여경(Yeo Kyeoung Kim),이연경(Youn Kyoung Lee),이균상(Kyun Sang Lee),조민석(Min Seok Cho),정택균(Taek Kyun Jeong),박병석(Byoung Seok Park),정균호(Gyun Ho Jeong),마성권(Seong Kwon Ma),김수완(Soo Wan Kim),김남호(Nam Ho Kim),최기철(Ki 대한내과학회 2002 대한내과학회지 Vol.63 No.3

배경 : 신이식 후에 발생하는 심혈관계 질환은 이식신의 손실과 함께 환자 사망의 주요한 원인으로 알려져 있다. 알려진 심혈관계 질환의 위험인자들로는 고지혈증, 고혈압, 당뇨병, 고령 및 흡연 등이 있으며, 또한 급성 거부반응의 유무, 좌심실 비대, C-reactive protein 등 염증 반응과 함께 고호모시스틴 혈증 등이 보고되고 있다. 호모시스틴은 신장 사구체를 통하여 배설되는 아미노산으로 말기 신질환 및 신이식 환자에서의 심혈관계 질환의 위험인자로 생각되고 있으나, 신이식 후 호모시스틴 농도의 변화 및 엽산과 비타민 보충 요법 등의 치료 효과는 아직 확립되어 있지 않다. 본 연구에서는 말기 신질환 환자들에서 신이식을 시행한 후 고호모시스틴 혈증의 발생과 이에 영향을 미치는 인자들에 대해 조사하였다. 대상 및 방법 : 정상 대조군 21명, 만성 신부전으로 최소 2개월 이상 치료 중인 환자 중 만성 신부전 보존 치료군 37명과 신이식 환자 48명을 대상으로 혈청 호모시스틴 농도와 혈청 호모시스틴 농도에 영향을 미치는 인자 등을 조사하였다. 결과 : 고호모시스틴혈증(정상대조군의 95백분위수 14.54 μmol/L 이상)의 유병율은 정상 대조군, 만성 신부전 보존 치료군과 신이식 환자군에서 각각 4.8%, 83.8%, 45.8%였다. 신이식 환자군 중 고호모시스틴 혈증은 정상 신기능군(혈청 크레아티닌 농도 남: 1.2 mg/dL, 여: 1.1 mg/dL 이하)과 비정상 신기능군에서는 각각 18.8%, 59.4%였다. 신이식 환자군(16.38±6.48 μmol/L)에서의 혈청 호모시스틴 농도는 정상 대조군(8.80±2.07 μmol/L)과 비교하여 유의하게 높았으나(p<0.01), 만성신부전 보존 치료군(24.68±9.01 μmol/L)과 비교하여 유의하게 낮았다(p<0.01). 또한 신이식 환자들 중 혈청 크레아티닌 정상군(12.02±3.68 μmol/L)에서 비정상군(18.57±6.51 μmol/L)에서의 혈청 호모시스틴 농도과 비교하여 유의하게 낮았다(p<0.01). 다중 회귀 분석상 신이식 환자들에서 혈청 호모시스틴 농도에 영향을 주는 독립적 인자는 혈청 크레아티닌 농도이었으며, 전혈 사이클로스포린 농도나 비타민 및 엽산 보충 요법의 유무와는 무관하였다. 결론 : 본 연구에서 신이식 환자의 혈청 호모시스틴 농도는 정상인에 비하여 유의하게 높았으나 보존 치료 중인 말기 신부전 환자와 비교시 유의하게 낮았다. 혈청 호모시스틴 농도에 영향을 주는 인자는 혈청 크레아티닌 농도였다. 또한 이러한 혈청 호모시스틴 농도의 감소가 신이식 환자에서의 심혈관 질환 발생의 감소에 기여할 것인지는 추후 연구가 필요할 것으로 사료된다. Background : Cardiovascular disease (CVD) after kidney transplantation is a major cause of both graft loss and patient death in kidney transplant recipeints. There are several well known risk factors of CVD, such as hyperlipidemia, hypertension, diabetes melitus, old age and smoking. Non-classic risk factors are acute rejection episode, LVH, C-reactive protein and hyperhomocysteinemia. Homocysteine is an amino acid filtered through the glomerulus and hyperhomocysteinemia is considered as a risk factor of CVD in end-stage renal disease (ESRD) and kidney transplant patients. So homocysteine lowering trials, such as folic acid and vitamine supplement therapy, are being made. We evaluated the prevelance and determinants of hyperhomocysteinemia in kidney transplant recipients. Methods : We measured serum total homocysteine concentration (tHcy) and its determinants in 21 normal persons, 37 chronic renal failure (CRF) patients with conservative treatment (predialysis) and 48 kidney transplant patients. Results : The prevalence of hyperhomocysteinemia was 4.8%, 83.8% and 45.8% among normal persons, predialysis and kidney tranplant patients, respectively. Among the kidney transplant recipients the prevelence of hyperhomocysteinemia was 18.8% in normal renal function (serum creatitine concentration male: below 1.2 mg/dL, female: below 1.1 mg/dL) group and 59.4% in abnormal renal function group. The tHcy values in kidney transplant patients are significantly lower than those in predialysis patients (16.38±6.48 μmol/L vs. 24.68±9.01 μmol/L, p<0.01), but higher than those in normal persons (16.38±6.48 μmol/L vs. 8.80±2.07 μmol/L, p<0.01). Among the kidney transplant recipients the tHcy values in normal creatinine group are significantly lower than those in abnormal creatinine group (12.02±3.68 μmol/L vs. 18.57±6.51 μmol/L, p<0.01). Using muliple regression analysis, this study showed increased serum creatinine concentration is a major determinant of tHcy concentrations in kidney transplant recipients and hyperhomocysteinemia is not correlated with whole blood trough level of cyclosporin (mean 126.26±62.19 ng/mL, range: 26∼322 ng/mL) or vitamines supplement therapy. Conclusion : In this study the serum homocysteine values in kidney transplant recipients were higher than in normal control group but significantly lower than in CRF patients with conservative treatment. The major determinant for serum homocysteine concentration is a serum creatinine concentration.(Korean J Med 63:306-313, 2002)

Polyunsaturated Fatty Acids(PUFAs) Content and Oxidation State of Marine Omega-3 Supplements in Chungcheongnam-do

Doe-Kyoung Kwon,Seoung-Soon Yeo,Nam-Woo Kim,Heun-Kag Kang 한국식품영양과학회 2022 한국식품영양과학회 학술대회발표집 Vol.2022 No.10

Medium Optimization for Pediocin SA131 Production by Pediococcus pentosaceus SA131 against Bovine Mastitis Using Response Surface Methodology

Park, Yeo-Lang,Lee, Na-Kyoung,Park, Keun-Kyu,Park, Yong-Ho,Kim, Jong-Man,Nam, Hyang-Mi,Jung, Suk-Chan,Paik, Hyun-Dong Korean Society for Food Science of Animal Resource 2010 한국축산식품학회지 Vol.30 No.1

Pediococcus pentosaceus SA131 was isolated from jeotgal, is the bacteriocin producer against bovine mastitis pathogens, Streptococcus uberis E290, Enterococcus gallinarum E362, and Staphylococcus epidermidis ATCC 12228. The medium composition for pediocin SA131 production by P. pentosaceus SA131 was optimized using response surface methodology. Component of medium was studied as carbon source (glucose, fructose, lactose, glycerol, sucrose, maltose, and mannitol), nitrogen source (beef extract, yeast extract, peptone, malt extract, and tryptone), mineral and surfactant ($MgSO_4$, $KH_2PO_4$, $(NH_4)_2SO_4$, $MnSO_4$, NaCl, sodium acetate, and Tween 80). Through one factor-at-a-time experiment, glucose, fructose, yeast extract, malt extract, NaCl, $MgSO_4$, and Tween 80 were determined as the good ingredient. The effects of major factors for pediocin SA131 production were investigated by two-level fractional factorial designs (FFD). By a $2^4$ FFD, fructose, yeast extract, and $MnSO_4$ were found to be the important factors for the bacteriocin production. Subsequently, a $2^3$ central composite design (CCD) was adopted to derive a statistical model for optimizing the composition of the fermentation medium. The estimated optimum composition for the production of pediocin SA131 by P. pentosaceus SA131 was as follows; 0.13% fructose, 1% glucose, 1.8% yeast extract, 2.58% $MnSO_4$, 0.2% NaCl, and 0.2% Tween 80. The pediocin production under optimized medium was increased to 1,000 AU/mL, compared to the 400 AU/mL in MRS medium.

온수온돌 난방시스템의 실-관망 통합시뮬레이션을 위한 유량분배 해석에 관한 연구

양경운(Yang Kyoung-Wn),이규남(Rhee Kyu-Nam),류성룡(Ryu Seong-Ryong),여명석(Yeo Myoung-Souk),김광우(Kim Kwang-Woo) 한국건축친환경설비학회 2007 한국건축친환경설비학회 학술발표대회 논문집 Vol.- No.-

In order to analyze the thermal performance of a hydronic radiant floor heating system, a hydronic analysis as well as a thermal analysis should be conducted for more accurate results. Room air temperature (determined by thermal simulation) and is influenced by water flow rate (calculated by hydronic simulation), and vice versa. Neverthless, thermal and hydronic analysis have been conducted separately because of the difference in analysis method. In general, a thermal simulation used to assumed constant flow rate to each room. In this study, a hydronic simulation code was developed using Matlab/Simulink. And a thermal simulation for a multi-zone esidential building was complemented by using the developed hydronic simulation code.

실별유량해석을 고려한 바닥복사난방 공간의 열성능 시뮬레이션에 관한 연구

양경운(Yang Kyoung-Wn),이규남(Rhee Kyu-Nam),류성룡(Ryu Seong-Ryong),여명석(Yeo Myoung-Souk),김광우(Kim Kwang-Woo) 대한건축학회 2008 대한건축학회논문집 Vol.24 No.12

In order to analyze the thermal performance of a hydronic radiant floor heating system, a hydronic analysis as well as a thermal analysis should be conducted for more accurate results. Room air temperature (determined by thermal simulation) and is influenced by water flow rate (calculated by hydronic simulation), and vice versa. Nevertheless, thermal and hydronic analysis have been conducted separately because of the difference in analysis method. In general, a thermal simulation used to assumed constant flow rate to each room. In this study, the conditions for coupling thermal and hydronic simulation together were investigated. Based on these conditions, a coupled model was developed using Matlab/Simulink. And a thermal simulation for a multi-zone residential building was complemented by using the developed a coupled model.

자궁경부암에서 MAGE 3 유전자 산물의 발현

강태경(Tae Kyoung Kang),서남원(Nam Won Seo),김도형(Do Hyung Kim),안은모(Un Mo Ahn),여태홍(Tae Hong Yeo),김준홍(Jun Hong Kim),안선의(Sune Ie Ahn),김동휘(Dong Hwi Kim),박은동(Un Dong Park) 대한산부인과학회 2001 Obstetrics & Gynecology Science Vol.44 No.3

N/A Objective: The human MAGE 3 gene encodes tumor specific antigens that are recognized by autologue cytotoxic T lymphocytes (CTL). The MAGE 3 gene is expressed not only in melanoma but in the other malignant tumors as well. There is, however, little information on the expression of the gene in uterine cervical carcinomas. The author thus studied the expression of the MAGE 3 gene products in uterine cervical carcinoma and discuss the possibility of specific immunologic diagnosis using MAGE 3 gene products. Methods: The expression of MAGE 3 gene product in 17 normal tissues of the cervix, 32 cervical intraepithelial neoplasia (8 CINⅠ, 10 CINⅡ, 14 CIS), and 43 invasive cervical carcinomas was studied by immunohistochemistry using anti-MAGE 3 mAb 57B in paraffin sections. Results: No expression of MAGE 3 gene product was detected in normal cervical tissues and in cervical intraepithelial neoplasias. The expression of MAGE 3 gene product was detected in 30.2% (13/43) of invasive cervical carcinomas. The MAGE 3 gene product was stained as a cytoplasmic protein in cancer cells. No statistically significant differences were observed between MAGE 3 gene product expression status and clinicopathologic parameters. Conclusions: The MAGE 3 gene products was expressed in invasive cervical carcinoma tissues.