버섯의 갈변병 유발세균 P. tolaasii의 길항세균인 P. fluorescens의 분리 및 배지최적화

조남철,박범식,전억한 경희대학교 유전공학연구소 1991 遺傳工學論文集 Vol.3 No.-

버섯 갈변병 유발세균인 P. tolaasii에 길항성을 가진 P. fluorescens의 대량배양을 위하여 배지조성 및 배양의 최적조건을 확립하였다. 세포성장에 있어서 carbon 및 energy source인 glucose의 경우 30g/l일 때 세포농도가 가장 높았으며, yeast extract의 최적농도는 10g/l였다. 또한 균체의 구조합성에 있어 중요한 nitrogen source인 NH_(4)Cl과 (NH_(4))_(2)SO_(4)는 각각 1.0g/l와 0.1g/l일 때 세포성장이 가장 좋은 것으로 나타났고, sulfur source인 MgSO_(4)·7H_(2)O의 최적농도는 1.0g/l였다. 그리고 KH_(2)PO_(2)와 CaCl는 각각 1.0g/l와 0.1g/l일때 세포농도가 가장 좋았고, 온도 30℃, pH 6.0 그리고 초기 D.O가 68일 때 세포성장이 가장 높았으며, 균체 비증식속도(μ)와 생산성(P)도 높았다. P. fluorescens was studied in both batch and continuous culture in order to find out optimum conditions for cultivation. p. fluorescens is an aerobic bacteria and antagonistic to p. tolaasii which causes blotch disease on the mushroom cap. Cells of P. fluorescens were grown well on medium containing 30 g/ℓof glucose, whereas the growth was inhibited with the glucose concentration at higher than 30g/ℓ. The highest values of specific growth rate and productivities were obtained when using 10 g/ℓ of yease extract. Optimum concentrations of NH_(4)Cl and (NH_(4))_(2)SO_(4) for culture were found to be l.0g/ℓ and 0.1 g/ℓrespectively. The culture of MgS0_(4)·7H_(2)O was mostly about l.0g/ℓ as a sulfur source. It was also found that the cell concentrations were at the maximum level when grown on the medium containing l.0g/ℓ of KH_(2)PO_(4) and 0.lg/ℓ of CaCl_(2). Also, the optimum culture conditions were 30℃ and pH 6.0. Cultivation of P. fluorescens at high initial D.O value led to a decrease of bacterial productivity in batch culture. Maximum productivity was achieved at 68 for the initial D.O. value.

기능상실한 이식신의 절제

조원현,남창우,김천일,주일,박성배,김현철 大韓移植學會 1994 Korean Journal of Transplantation Vol.8 No.1

IPA-KOH 혼합액에 의한 습식 이방성식각 연구

천인호,조남인,김창교 호서대학교 반도체제조장비국산화연구센터 2000 학술대회 자료집 Vol.2000 No.1

이방성 습식 식각을 이용하여 멤브레인을 제작하기 위하여 KOH-IPA의 식각액을 사용하여 단결정 실리콘 기판을 이방성으로 식각을 하고, 각 용액에 대한 식각 특성을 관찰하였다. 식각률은 식각액의 온도와 농도에 의존하며, 패턴 형성 방향과 식각액의 농도에 따라 식각 형태가 다르게 나타났다. 패턴은 Primary Flat에 45˚로 기울여 형성되었으며 20wt·% KOH 80℃ 이상에서는 U-groove, 그 이하의 온도와 농도에서는 V-groove 식각 형태를 관찰할 수 있었다. 각 면에 식각률 차이에 의해서 생기는 Hillock은 온도와 농도가 높아짐에 따라 줄어들었고, 재식각을 통하여 현저하게 줄어듦을 알 수 있었다.

서남해안 지방에서 발생한 Vibrio vulnificus 감염의 임상적 고찰

조남수,김춘호,김성중,김용배,조수형,안태훈 大韓應急醫學會 1999 대한응급의학회지 Vol.10 No.2

Background: V. vulnificus infection in found between May and October when the man who has hepatic disorder or immunity disorder eats raw shellfish and it causes the systemic symptoms such as sudden fever, chilling, shock and stupor and local skin lesions like bleb and necrosis. This disease has 46∼61% of fatality rate in spite of intensive treatment. This study conducts the examination of history, epidemic study, ingesting raw fish and bacteriologic investigation in the patients with similar clinical symptoms to the above and examines the diagnostic dorrelations. Method: It is conducted with 31 cases who admitted at emergency medical center of Chosun University Hospital from May of 1995 to September of 1998 and are suspected to have V.vulnificus infection. It analyze bacteriologic examination, the survey of residence, eating raw shellfish, hepatic disorder, alcohol drinking habits and skin lesion. Results: 1) The rate of male and female is 15.5:1 and in the age, 18 cases are in fifties, seven are in forties and six are over sixties. 2) Clinically, V.vulnificus is detected in 20 of 31 cases which is suspected to have V.vulnificus infection and it is not detected in 5 cases. Other bacteria are detected in 6 cases and they include staphylococcus aureus, pseudomonas species, β-hemolytic streptococcus and E-coil. 3) In local distribution, Kohung has 10 cases, Shinan and Mokpo have six, Haenam and Kangjin have five, Yongkwang and Muan have five, Naju and Yongam have five and Kwangju has one. 4) Eating fishes and raw shellfishes is found in 31 cases. In the kind of shellfishes, eating raw clam is 10 cases, raw thin-shelled surf calm is 8 cases, raw oyster is 5 cases and sliced raw fish is 4 cases and pickled sea foods are 4 cases. 5) The cases with hepatic disorder are 23 and 15 cases with heavy alcohol drinking Carrier are included. The cases with Diabetic Mellitus are four and those with pulmonary tuberculosis are two. Two cases have not basal diseases. 6) On the opinion of skin lesion, 20 cases show vesicular necrosis, 7 cases have dendriform erythema, 3 cases have papule and one case doesn't show clear skin lesion. Conclusion: When V.vulnificus infection is detected, it is fatal and those who have hepatic disorder or heavy alcohol drinking habit must avoid eating fishes and shellfishes raw in summer. It is important to have emergency treatment for the patient whose V.vulnificus infection is suspected. It is considered that the administration of initial antibiotics is effective by generalizing the clinical symptom, epidemic opinion and patient history even before the results of bacteriologic confirmed.

메스암페타민 사용 환자의 정신과적 증상에 영향을 미치는 요인

이춘우,권성민,조성남,권도훈,임효덕,이성국 대한생물치료정신의학회 2004 생물치료정신의학 Vol.10 No.1

목 적 : 본 연구는 메스암페타민 남용 또는 의존 환자의 사회인구학적 특성과 이에 따른 동반된 정신장애를 알아보고, 메스암페타민 사용이 정신과적 증상에 미치는 영향을 알아보기 위함이다. 방 법 : 2002년 7월부터 2003년 7월까지 국립부곡병원 약물중독진료소에 입원한 환자 중 DSM-Ⅳ 진단 기준에 의하여 메스암페타민 남용 또는 의존으로 진단된 44명의 남자 환자를 대상으로 사회인구학적 변인과 물질 의존의 병력, SCL-90-R, BPRS, HRSD, YMRS, Impulsive scale 등을 측정하였다. 결 과 : 메스암페타민 사용 환자들의 93.2%가 우울하였으며, 59.1%는 치료가 필요한 정도의 우울증을 가지고 있었다. 20대 연령층에서는 대인예민성, 적대감 및 공포증에서 유의하게 높았고, 중졸 이하의 교육 수준에서는 대인예민성, 충동성에서 유의하게 높았으며, 무직인 경우는 강박증, 불만, 적대감 및 조중 척도에서 유의하게 높았다. 처음 사용한 연령은 사용 횟수, 유병 기간과 유의한 상관 관계를 보였으나, 수감 횟수와는 낮은 상관 관계를 보였다. 사용 횟수와 적대감, 공포증, 편집증은 서로 유의한 정상관을 보였다. 우울증 척도와 정신병 척도 및 충동성 척도는 서로 유의한 정상관을 보였다. 100회 미만보다 500회 이상 사용했을 경우에 불안, 적대감, 공포증 및 편집증에서 유의하게 높았다. 결 론 : 메스암페타민 남용 또는 의존 환자에서 우울증 유병률이 매우 높게 나타났고, 20대 연령층과 중졸 이하의 교육 수준 그리고 무직인 경우 정신과적 증상이 많이 동반되었으며, 사용 횟수에 따라 불안, 적대감, 공포증, 편집증에서 유의한 차이를 보였다. 그러므로 모든 메스암페타민 사용자에 대해 적절한 정신과적 평가가 필요할 것으로 생각한다. Objectives : The purpose of this study was to examine the sociodemographic characteristics and the comorbid psychiatric disorders and the effect of methamphetamine use on psychiatric symptoms in methamphetamine abuse or dependent patients. Methods : The study subjects were 44 hospitalized male patients who met the DSM-Ⅳ criteria for methamphetamine abuse or dependence. The study included items assessing the sociodemographic profiles, history of substance use, and psychiatric symptomatology. Psychiatric assessments were based on SCL-90-R, BPRS, HRSD, YMRS and Impulsive scale. Results : In methamphetamine use patients, 93.2% were depressed and 59% had moderate to severe depression There were significant differences in interpersonal- sensitivity, hostility and phobia in the 3rd decade age group, interpersonal-sensitivity and impulsive scale in .under the middle school education group, and obsession-compulsion, anxiety, hostility and. YMRS in no-job group, respectively compared to the other groups. Age of the first use was correlated with the frequency of use, and also with the duration of illness, but not with the number of incarceration. There were positive correlations among HRSD, BPRS and impulsive scale. In the above 500 times used group there were significant differences in anxiety, hostility, phobia and paranoia compared to the below 100 times used group. Conclusion : In methamphetamine abuse or dependent patients, the incidence rate of comorbid depression was 93.2%. In the 3rd decade age group, under the middle school education group and no-job group showed higher diverse psychiatric symptoms compared to the other groups. Frequency of use was positively correlated with the symptoms such as anxiety, hostility, phobia and paranoia. We suggest that relevant psychiatric evaluations are needed for all methamphetamine users.

Web 인지도를 반영한 전자상거래 마케팅 채널 경쟁에 관한 연구

차춘남,조형래 한국경영과학회 2000 經營 科學 Vol.17 No.3

The proliferation of electronic commerce(EC) has led manufacturers to consider internet based marketing as a salient candidate for strategic diversification of marketing channel. In this case, each manufacturer can build its own Web store or rent an existing special EC store. Such decision making of 'build' or 'rent' can be analyzed by a game model which derives the Nash solutions for price and profit considering degree of competition with other competitive manufacturers. In this paper, to overcome the drawbacks of the traditional linear demand function, we first propose a new linear demand function which incorporates not only the price difference between competitive products but the awareness of Web stores perceived by the consumers, then design the game models to analyze the characteristics of three typical types of marketing channel in duopoly market. Based on the Nash solutions of the game models, we analyzed the effect of the degree of competition and Web awareness in selecting the optimal marketing channel.

급성 임파성 백혈병의 말초혈액 검사소견

김성집,안경남,조무자,김춘식,심상인,김선무 대한임상병리사협회 2001 대한임상병리사회지 Vol.21 No.1

익수환아의 예후에 영향을 미치는 인자에 관한 연구

김용배,정대봉,조수형,조남수,박영봉,박상기,김춘호,양은석,문경래 大韓應急醫學會 1998 대한응급의학회지 Vol.9 No.3

Drowning is the second most common cause of accidental death of children in Korea. But the study of near-drowning is very rare in Korea. The purpose of our study is to determine the factors that may influence survival on the basis of submersion time, consciousness state upon admission to hospital, the kinds of water, laboratory findings and neurological outcomes. The survey was performed by a retrospective cohort study on 28 near-drowning victims of less than 15 years of age who were admitted to the emergency room in the Chosun University Hospital between May 1988 & May 1997. The results were as follows :1)Sex distribution was 22 males and 6 females. 2) Submersion time was <5 minutes in 9 cases, 5-9 minutes in 6 cases, 10-14 minutes in 5cases, 15-19 minutes in 3 cases, ≥20 minutes in 5cases. 3) Comatose patients upon arrival were 6/7 cases in the death group(86%) and 2/21 cases in the improved group(10%). They had unfavorable outcomes(P<0.05). 4) The first PH value was mean 7.02±0.12 in the death group and mean 7.31±0.13 in the improved group. There was a statistically significant difference between the death and the improved group(P<0.05) 5) The patients who gad increased blood glucose concentration were all 7 cases in the death group and 12/22 cases in the improved group(54%). The mean blood glucose concentration was mean 424.7235.6㎎/㎗ in the death group and mean 140.182.7㎎/㎗ in the improved group. There was a statistically significant difference between the death and the improved group(P<0.05). 6) The patients who had pulmonary edema upon arrival were all 7 cases in the death group and 1/21 cases in the improved group(46%). They had unfavorable outcomes(P<0.05). 7) The patients who had been submerged more than 15 minutes were all 7 cases in the death group and 6/21 cases in the improved group(5%). They had unfavorable outcomes(P<0.05). We conclude that pediatric victims of near-drowning can be assigned to high or low likelihoods of unfavorable our outcomes with the use of five variables ; comatose mentation upon arrival decreased initial blood pH, increased initial blood glucose concentration, pulmonary edema, and maximum submersion time estimated longer than fiften minutes. This prediction rule may be useful if it can be validated in another cohort.

카리스마적 리더십에 관한 연구

박종태,최영곤,한남익,박철용,표내숙,조춘호 釜山大學校 附設 體育科學硏究所 1998 體育科學硏究所 論文集 Vol.14 No.-

In the early decades of this century, Charisma which originally means endowment with the gift of grace, has been introduced to social sciences by German sociologist, Max Weber. It quickly became interested in the field of sociology and politics. But, into the 1970s, charismatic leadership still had not became a subject for empirical or experimental research related to organizational theory. Recently, much attention has been paid to charismatic leadership as the prototype of leadership that people have in mind when they describe their ideal leader and is more likely to provide a role model with which subordinates want to identify. As a result, in will perform further study related to Charisma leadership which developing various kind of measurement and instrument. Finally, it should identify lost of possibility and phenomenon through the study of charisma leadership.

경부 관통상으로 인한 사지 마비 1례

박 진,이경운,김춘호,김성중,조남수,안태훈 大韓應急醫學會 1998 대한응급의학회지 Vol.9 No.3

A case of quadriplegia resulted from cervical penetrating wound was admitted to chosun university hospital intensive care unit, and undergone steroid therapy and antibiotics therapy. On the 6th hospital day, the septic complication was developed and it proved to be bacterial meningitis by CSF examination. Broad spectrum antibiotics was administered immediately after and the diagnosis and the patient was monitored carefully. The bacterial meningitis was improved and no other serious complication was developed. The patient was discharged with permanent neurologic deficit, quadriplegia.