Slide Session : OS-IFD-07 ; Infectious Disease : In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus

( Myung Jin Lee ),( Kye Hyung Kim ),( Jong Youn Yi ),( Su Jin Choi ),( Chung Jong Kim ),( Nak Hyun Kim ),( Kyoung Ho Song ),( Pyoeng Gyun Choi ),( Ji Hwan Bang ),( Wan Beom Park ),( Eu Suk Kim ),( San 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus Myung Jin LEE1, Kye-Hyung KIM1, Jongyoun YI2, SuJin CHOI1, Chung-Jong KIM1, Nak- Hyun KIM1, Kyoung-Ho SONG1, Pyoeng Gyun CHOI1, Ji-Hwan BANG1, Wan Beom PARK1, Eu Suk KIM1, Sang-Won PARK1, Hong Bin KIM1, Nam Joong KIM1, Myoung- Don OH1 Seoul National University College of Medicine, Korea1, Pusan National University School of Medicine, Korea2 Background: Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease caused by a novel Bunyavirus, severe fever with thrombocytopenia syndrome virus (SFTSV). No effective antiviral therapy is proven yet, but clinical use of ribavirin (RBV) has been tried. We investigated the antiviral effect of RBV against SFTSV in vitro. Methods: To test for cytotoxicity of RBV, Vero cells were treated with different concentrations of RBV (3.90 to 500 μg/mL, two-fold dilution) and analyzed by cell viability MTS assay 48h post-infection. To determine antiviral activity of RBV against SFTSV, Vero cells were infected with SFTSV strain Gangwon/Korea/2012 at 100 TCID50 (50% tissue culture infective dose) per well in a 96-well plate, and RBV was added at the concentrations showing no or minimal cytotoxicity. Viral RNAs were extracted from the culture supernatants and quantifi ed using one-step real-time reverse transcription- PCR to amplify the partial large segment of SFTSV. Statistical analysis was done by one-way ANOVA with Tukey`s post hoc test. Results: Cytotoxicity due to RBV was not observed at RBV concentration =31.3 μg/ mL. Viral RNAs at 24h post-RBV treatment were reduced with increasing RBV concentrations (1-32 μg/mL), compared with those of mock-treated cells (P <0.01, Figure). Half maximal inhibitory concentration (IC50) of RBV was 3.69 μg/mL at 24h post-RBV treatment. Conclusions: Our study shows that RBV has antiviral effect against SFTSV in a dose-dependent manner. Further studies are required to evaluate the effi cacy of RBV in SFTS.

ERK1/2 activation in quercetin-treated BEAS-2B cell plays a role in Nrf2-driven HO-1 expression

이윤진,Jun-Hwan Song,Myung-Ho Oh,Yong-Jin Lee,김용배,Jae-Hyuk Im,이상한 대한독성 유전단백체 학회 2011 Molecular & cellular toxicology Vol.7 No.4

Quercetin, a member of the flavonoids,has been heralded as chemopreventive agent, generally because of its free radical-scavenging and antiproliferative activities. In this process, NF-E2-related factor-2 (Nrf2) plays a role in protecting cells from oxidative damage; however, the exact mechanisms in which bronchial epithelial cells respond to quercetin by activating Nrf2 are not fully understood. Here, we report the role of extracellular signal-regulated kinase (ERK) activation in response to quercetin. Quercetin treatment decreased the viability of human bronchial epithelial BEAS-2B cells in a dose- and time-dependent manner. ERK1/2 was activated within 10 min of quercetin addition and then remained increased above control level throughout 48 h, whereas AKT phosphorylation was suppressed from the first 1 h of quercetin treatment. Inhibition of Erk1/2 phosphorylation attenuated quercetin-induced loss of cell viability. Nrf2levels in both nuclear and whole cell lysates increased after quercetin treatment and was accompanied by the increase of heme oxygenase-1 (HO-1) protein level,indicating that increased HO-1 expression is Nrf2-mediated. Activation of the Nrf2/HO-1 system after quercetin treatment was suppressed by the pretreatment with MEK inhibitor, PD98059. Also, silencing Nrf2 with siRNA decreased cell viability and augmented cytotoxic effect of anticancer drug LBH589. Overall, our results indicate that quercetin induces the up-regulation of Nrf2-mediated HO-1 expression, at least in part, via MEK/ERK1/2 signaling, as an adaptive mechanism which may provide advantages for cell proliferation and enhance survival despite endogenous and/or exogenous oxidative insults.

배관망 해석 방법을 이용한 스프링클러 시스템의 수리계산 프로그램 개발

송철강,이명호,강계명 한국화재소방학회 2002 한국화재소방학회논문지 Vol.16 No.1

본 연구는 배관에서의 정확한 압력손실과 유량을 측정하기 위하여 스프링클러 시스템중 격자배관방식에 대한 수리계산 프로그램을 개발하는데 그 목적이 있다. 본 프로그램 개발은 배관망 해석방법 등 여러 가지 자료로 근거하며, 격자배관방식이 국내에서 현행 시행되고 있는 규약배관방식의 단점을 보안하여 스프링클러 시스템을 성능기준화재안전설계로 이끌어 내기 위함이다. 소화설비의 전산화로 인한 작업의 편리성과 정확한 계산을 측정함으로서 전진적인 화재안전설계를 이룩하게 될 것이다. 이러한 프로그램의 개발은 미국 등에서 먼저 이루어져 오고 있으며 국내에서의 소방에 대한 발전이 한 단계 발전되는 계기를 마련하게 될 것이다. The purpose of this study is developing the computer program for hydraulic design sprinkler systems have performed the means for the general use of network analysis method. The computer program is based on the theoretical concept of the related Hazen-Williams equations, a modified Bernoulli equations, and the Hardy Cross method of pipe network analysis. Looped piping calculations are solved by using either the Hardy Cross method or the other iteration methods. While the other methods are solved using simultaneous equations, the Hardy Cross method is concerned with one loop at a time using reiterative process. Due to its simplicity the Hardy Cross method will be the primary method described in this thesis. The purpose of this study is to develope hydraulic calculation program by using algorithm for network analysis method. The development of computer program for the hydraulic design of sprinkler systems will perform the means in the performance-based sprinkler system design.

야생너구리(Nyctereutes procyonoides)의 혈액 및 혈액화학치

송근호,유명조,김덕환 충남대학교 수의과대학 동물의과학연구소 2002 動物醫科學硏究誌 Vol.10 No.-

This study was performed to determine the blood and blood chemical values on captured 16 raccoon dogs (Nyctereutes procyonoides). Hematological examination revealed that RBC, Hb, PCV, MCV, MCH and MCHC values were 7.9±0.8x10^(6)/㎕, 14.0±2 g/dl, 44.1±5.5 %, 56.9±3.8 fl, 18.0±1.4 pg and 31.9±2.9 g/dl. respectively, and also revealed that WBC, neutrophil, lymphocyte. eosinophil and monocyte values were 13.6±2.4x10^(3)/㎕, 8.9±2.1x10^(3)/㎕, 2.7±0.8x10^(3)/㎕, 1.1±0.5x10^(3)/㎕ and 1.0±0.4x10^(3)/㎕, respectively. Blood chemical examination showed that total protein, albumin, globulin and A/G ratio values were 7.9±0.9 g/dl, 3.0±0.3 g/dl, 4.8±0.7 g/dl and 0.6±0.1, respectively, and also showed that ALT, AST, BUN. Creatinine and glucose values were 95.8±57.8 1U/ℓ, 64.4±30.1 1U/ℓ, 20.8±5.4 ㎎/dl, 0.7±0.1 ㎎/dl and 58.5±19.1 ㎎/dl, respectively.

개에서 교통사고에 의한 폐좌성 1례

송우리,이시예,신에스더,박희서,정성목,송근호,김명철 충남대학교 수의과대학 동물의과학연구소 2004 動物醫科學硏究誌 Vol.12 No.-

A 4 years old, mixed male dog with a history of traffic accident was presented to the Veterinary Medical Teaching Hospital of Chungnam National University with tachypnea and respiratory distress. Physical examination showed that the dog was auscultated crackles sounds, dysynchronous abdominal breathing pattern, hemoptysis, hematuria, urodynia and right and left scleral congestion. Blood and blood chemical examination revealed that WBC, AST and ALT was highly elevated. Parenchymal pulmonary fluid and closed pneumothorax were observed by thoracic radiography. This case was diagnosed as pulmonary contusion. Intravenous fluid and oxygen was administered and also dexamethasone(1mg/kg, Ⅳ), furosemide(2mg/kg, Ⅳ), (ampicillin, 20mg/kg, Ⅳ) and meloxicam(0.2mg/kg, PO) were intravenously injected for 5 days. Abnormal clinical signs were disappeared after treatment.

전압제어 GIC를 이용한 대역 및 고역통과 필터의 특성 비교

송명석,노경호,김왕식,박종연 강원대학교 정보통신연구소 2004 정보통신논문지 Vol.8 No.-

In this paper, we have presented comparative characteristics of band-pass filter and high-pass filter using VCGIC(Voltage Controlled Genenalized Impedance Converter), which are the non-linearized VCR(Voltage Controlled Resistance) and the linearized VCR. We concluded that the linearized VCGIC circuit has the wide dynamic range and the improved linearity.

전류측정에 의한 AC서보모터의 언밸런스 고장검출

송명현,조병호,김부열 순천대학교 2000 순천대학교 자연과학논문집 Vol.19 No.1

Using current measurement, the analytic method for the unbalance fault detection of 3Φ induction servomotor was proposed. The fault detection of the load unbalance has been studied by means of the measure of vibration. In the conventional method, the additional vibration sensors are set up to measure the vibration properly, but the method introduced in this paper, a current sensor for motor control is used for measuring the load current, so there is no need for additional sensors. After measuring the current caused by unbalance load, the FFT spectrum of the measared current were analyzed and compared with the spectrum pattern of the balanced load. Varying the motor speed by 500, 1000, 1500 and 2000 rpm, some characteristic frequency for detecting the unbalance fault were found. These experimental results show that the proposed current measuring method is more simple, easy, and effective than the conventional vibration measuring method.

중국의 한국기업 투자환경분석 : 동북3성을 중심으로

김명호,송영훈 江陵大學校統一問題硏究所 1997 統一問題硏究 Vol.13 No.-

하치조신경 및 설신경 손상 평가를 위한 한국인 정상 성인의 하순-이부 및 혀의 감각 조사

이종호,이세영,송승일,이은진,안강민,김성민,명훈,황순정,서병무,최진영,정필훈,김명진 大韓顎顔面成形再建外科學會 2003 Maxillofacial Plastic Reconstructive Surgery Vol.25 No.2

In the head and neck area, there are so many sensory nerves, which are sometimes injuried iatrogenically or inadvertently so that involved patients complained of the loss of sensations. In such cases, it is important to judge the degree of injuries and regeneration of nerve for better diagnosis and treatment. Seddon and Sunderland's classification, which is commomly used, is focused on histological change and nerve conduction. As times goes by, it is difficult to access patient's sensory disturbance by this method. Until now, so many methods such as contract threshold, direction, two-point discrimination, pin prick, thermal discrimination and current perception threshold have been introduced for sensory evaluation. However, there hasn't been enough information regarding each methodology nor integrated standard methodology for the measurement. the purpose of this study is to get Korean adult normative sensory values of lower lip,chin and tongue using modified methods of contact thershold, ditection, two point discrimination, pin prick, thermal discrimination and assess degree of regeneration of sensory nerve damage.

후천성 면역 결핍증 환자의 혀에 발생한 거대세포바이러스(CMV : cytomegalovirus) 감염

송노헌,이종호,정필훈,김명진,김수경,남일우,김종원,백세미,홍삼표 大韓顎顔面成形再建外科學會 1998 Maxillofacial Plastic Reconstructive Surgery Vol.20 No.1