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신장 상피세포주 A498을 이용한 대황(Rheum undulatum L.) 추출물의 세포독성
나명석,진종언,조남철 한국식품영양학회 2000 韓國食品營養學會誌 Vol.13 No.5
본 연구에서는 전통적으로 담석증, 신장치료 등의 한약제로 많이 사용하는 대왕을 여러 용매를 사용하여 얻은 대왕추출물 분액에 대한 세포독성을 여부를 MTT 정량법, NR 정량법, SRB 정량법을 이용하여 조사하였다. 1. 추출 용매 methylene chloride, ethyl acetate, butanol, water로부터 얻은 대왕추출물 모두 처리농도에 따라 세포에 미치는 영향이 증가하였다. 2. Butanol을 용매로 사용하여 얻은 대왕추출물 분액이 다른 3가지 용매로부터 얻은 대왕추출물보다 세포에 미치는 영향이 크게 나타났고 water를 용매로 사용하여 얻은 추출물이 A498세포주에 미치는 영향이 가장 낮게 나타났다. 3. Butanol을 추출 용매로 하여 얻은 대왕추출물이 A498세포주에 미치는 영향이 가장 컸는데 그 추출물에 대한 MTT_50, NR_50, SRB_50값은 각각 0.63㎎/m1, 0.65㎎/m1, 0.68㎎/m1이었고, 가장 영향이 적은 water의 경우 MTT_50, NR_50, SRB_50값은 각각 0.84㎎/m1, 0.82㎎/m1, 0.80㎎/ml이었다. 4. 정량방법 간의 대왕추출물에 대한 반응은 MTT 정량법이 가장 민감하게 나타났다. We have evaluated cytotoxic effects of four crude extracts of methylene chloride, ethyl acetate, butanol, water layer isolated Rheum undulatum in A498 cell line, human kidney epithelial cells. The cytotoxic evalutation was measured by colorimetric assay using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT), neutral red (NR) and sulforhodamine protein B (SRB) . These results obtained are as follows : MTT, NR and SRB quantities were significantly decreased in cultured A498 cells treated four crude extracts by increased concentrations. The cell cytotoxic effect of crude extracts of butanol layer was more stronger than others layer. The values of MTT_50, NR_50, SRB_50 of crude extract of butanol layer and were measured both 0.63 ㎎/ml, 0.65 ㎎/ml, and 0.68 ㎎/ml, respectively and the values of water layer were 0.84 ㎎/ml, 0.82 ㎎/ml, and 0.80 ㎎/ml, respectively in cultured A498 cell line.
급냉 응고된 Al-Li-Cu합금의 열처리, 미세조직 및 기계적 성질에 관한 연구(I)
許明日,南勝義,李範圭,羅亨用,金錫胤 弘益大學校 1988 弘大論叢 Vol.20 No.2
Effect of aging condition on the microstructure and hardness properties of Al-3Li, Al-3Li-2Cu, Al-3Li-2Cu-0.3Zr have been studied. The alloy ribbons have been prepared by means of Single Roll Melt Spinning by rapid solidification technique. In rapidly solidified Al-3Li-2Cu, Al-3Li-2Cu-0.3Zr alloy ribbons show the icosahedral intermetallic compound of five-fold symmetry which is placed in cell-bound-ary and matrix. In the matrix precipitated fine δ'(Al₃Li) phase. Heat-treated at 250℃ 1hr. In this condition, δ'(Al₃Li) phase started decompose. The icosahedral intermetallic compound has been grown. When heat-treated at 300℃ 1 hr, the δ' phase has been decomposed all. In the matrix, icosahedral intermetallic compound has been grown greatly. The micro-hardness has been obtained more high Al-Li-Cu-(Zr) alloy than Al-Li alloy. Strength-hardended phenomena showed to solution hardning by the addition of Cu. When the heat-treated at 250℃ 1 hr, in the alloy of Zr addition, delayed decomposed δ' phase. In the case, micro-hardness gradually decreased. When the heat-treatment have done at 300℃ 1 hr, the micro-hardness has been decreased because δ' phase decomposed all.
양파(Allium cepa L.) 추출물이 Tyrosinase 유전자 발현에 미치는 효과
조남철,윤연희,이혜진,손현정,김양경,최근희,나명석,조영권,이황희,진종언 한국식품영양학회 2001 韓國食品營養學會誌 Vol.14 No.3
Melanin 생성에 관여하는 tyrosinase 유전자의 발현을 억제하는 물질을 탐색하고자 tyrosinase promoter를 지닌 Bl6 mouse melanoma cell해 앙파 methanol 추출물을 처리한 바 양파 methanol 추풀물은 10.0㎍/ml, 100.0 ㎍/ml, 1.0 ㎎/ml의 농도에서 대조군에 비해서 약 15%, 23% 57%의 억제효과를 나타냈으며 세포생존율은 1.0 ㎍/ml, 10.0 ㎍/ml, I00.0 ㎍/ml, 1.0㎎/ml의 농도에서 약 126 %, 92%. 85%, 64%로서 세포독성이 낮게 나타났다. Ethyl acetate, butyl alcohol, 그리고 물 용매 분획물은 tyrosinase 유전자의 발현물 억제하는 효과가 없었지만 methylene chloride 용해 분획물은 10.0 ㎍/ml과 100.0 ㎍/ml의 농도에서 약 37%와 35%의 발현율을 나타냄으로서 대조군에 비해 크게 억제하였다. Onion(Allium cepa L,) extract by methanol repressed the expression of tyrosinase gene of B16 mouse melanoma cell containing tyrosinase promoter. 10.0 ㎍/ml, 100.0 ㎍/ml, 1.0 ㎎/ml of the extract repressed expression of tyrosinase gene about 15%, 23%, and 57%, respectively, compared with control. In the MTT assay, the same extract exhibited low cytotoxicity at 1.0 ㎍/ml, 10.0 ㎍/ml, 100.0 ㎍/ml, and 1.0 ㎎/ml, respectively. The fractions of ethyl acetate, butyl alcohol, and water did not showed the repressive effect on the expression of tyrosinase gene, but the fraction of methylene chloride repressed highly at 10.0 ㎍/ml and 100.0 ㎍/ml.
아므르불가사리 (Asterias amurensis)의 배우자를 이용한 남해해역 연안해수의 생물학적 수질평가
유춘만,박지인,조기안,주현수,박종천,나명석 한국환경과학회 1999 한국환경과학회지 Vol.8 No.1
This study was carried out to evaluate off-shore water quality and to draw marine pollution map to Southern sea in Korea by bioassay using gametes and early development system of Asterias amurensis. From the bioassay, it was determined that the water qualites of Southern sea maintained the grade Ⅱ, because development of A. amurensis were moderately inhibited. However, Sunchon, Kwangyang, and Jinhae Bay belonged to grade Ⅲ, because development of A. amurensis were strongly inhibited. This could be due to the inflows of several river systems and poor water circulation. The water quality at Yosu island was the grade I, because A. amurensis were not inhibited in its early development.
생육 시기별 탱자 에탄올 추출물의 항산화 및 항균 효과
전라합 ( Ra Hap Jeon ),최희진 ( Hee Jin Choi ),문승재 ( Seung Jae Moon ),나명석 ( Myung Suk Na ) 한국미용학회 2010 한국미용학회지 Vol.16 No.4
This study was to examine the effect of antioxidant and antimicrobial activity with 99% ethanol extracts of Poncirus trifoliata during growth. Antioxidant activity of Poncirus trifoliata onto free radical generation was determined by measuring DPPH and ABTS radical scavenging activities in vitro. Antimicrobial activity was tested against Escherichia coli Streptococcus aureus, Propionibaterium acnes, Malassezia furfur. Antioxidant activities of ethanol extracts of Poncirus trifoliata was 87.16% in the DPPH assay and 28.93% in ABTS assay at 2.0 mg/g. Antimicrobial activity was that Propionibaterium acnes was higher than others microorganism. These results demonstrate that unripe blue Poncirus trifoliata may be useful in the materials of cosmetics.